Antibacterial and anthelmintic activities of Xanthium strumarium (Asteraceae) extracts
Keywords:
fruits; leaves; stem; root; growth inhibition zone; bacterial colonies; multi-resistant strain; nematodes
Abstract
Plants of the Asteraceae family are broadly used against microorganisms pathogenic to people and animals, although their potential has not been studied completely so far. In an in vitro experiment, we tested ethanolic, ethyl-ether, and dimethyl-sulfoxide extracts from the fruits, leaves, shoots, and roots of Xanthium strumarium L. for their effects on 13 species of bacteria and larvae of 3 species of nematodes. Over-8-mm-wide growth-inhibition zones of the colonies around disks saturated with ethanolic extracts from the fruits were observed for 11 bacteria: Enterobacter cloacae, Klebsiella pneumonia ssp. rhinoscleromatis, K. pneumonia ssp. ozaenae, K. aerogenes, Salmonella enterica, Escherichia coli, Morganella morganii, Enterococcus faecalis, E. faecium, Staphylococcus aureus, and Bacillus subtilis. Ethanol extract from the leaves inhibited growth of the colonies of 10 bacteria: E. cloacae, K. pneumonia ssp. rhinoscleromatis, K. pneumonia ssp. ozaenae, S. enterica, E. coli, Proteus vulgaris, E. faecalis, E. faecium, S. aureus, and B. subtilis. Ethanol extract from the stem inhibited growth of the colonies of only 6 bacteria: K. aerogenes, E. cloacae, P. vulgaris, E. faecium, S. aureus, and B. subtilis. Ethanol extract from the root inhibited growth of 10 bacteria: E. cloacae, K. pneumonia ssp. rhinoscleromatis, K. pneumonia ssp. ozaenae, K. aerogenes, S. enterica, E. coli, Pseudomonas aeruginosa, E. faecalis, E. faecium, and S. aureus. We analyzed the effects of extracts based on ethyl ether and dimethyl sulfoxide, finding no antiparasitic activity or expressed anthelmintic properties of various extracts from the fruits, leaves, stem, and roots of X. strumarium against larvae of the nematodes Strongyloides papillosus, Haemonchus contortus, and Muellerius capillaris.References
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Azimova, S. S., & Glushenkova, A. I. (2012). Xanthium strumarium L. In: Azimova, S. S., & Glushenkova, A. I. (Eds.). Lipids, lipophilic components and essential oils from plant sources. Springer Nature. P. 143.
Bengtsson-Palme, J., Angelin, M., Huss, M., Kjellqvist, S., Kristiansson, E., Palmgren, H., Larsson, D. G. J., & Johansson, A. (2015). The human gut mi-crobiome as a transporter of antibiotic resistance genes between continents. Antimicrobial Agents and Chemotherapy, 59(10), 6551–6560.
Boyko, A. A., & Brygadyrenko, V. V. (2016). Influence of water infusion of medi-cinal plants on larvae of Strongyloides papillosus (Nematoda, Strongyloididae). Visnyk of Dnipropetrovsk University, Biology, Ecology, 24(2), 519–525.
Boyko, O. O., & Brygadyrenko, V. V. (2019). Nematocidial activity of aqueous solutions of plants of the families Cupressaceae, Rosaceae, Asteraceae, Faba-ceae, Cannabaceae and Apiaceae. Biosystems Diversity, 27(3), 227–232.
Boyko, O. O., Kabar, A. M., & Brygadyrenko, V. V. (2020). Nematicidal activity of aqueous tinctures of medicinal plants against larvae of the nematodes Strongy-loides papillosus and Haemonchus contortus. Biosystems Diversity, 28(1), 119–123.
Boyko, O., Shendryk, L., Shaban, O., & Brygadyrenko, V. (2021). Influence of essential oils on sporulation of Eimeria magna oocysts. Annals of Parasitology, 67(1), 11–17.
Cesur, C., Coşge Şenkal, B., & Uskutoğlu, T. (2022). Fatty acid composition of seed oil from cocklebur (Xantium strumarium subsp. strumarium) grown in Turkey. Cumhuriyet Science Journal, 43(3), 379–383.
Chavan, S. T., & Kulkarni, A. U. (2021). Morphological and phytochemical studies on Xanthium strumarium L. Plantae Scientia, 4(6), 287–290.
Devkota, A., & Das, R. K. (2018). Antibacterial activities of Xanthium strumarium L. Journal of Natural History Museum, 29, 70–77.
Doğan, G., & Kıran, Y. (2017). Karyotype analysis of common cocklebur (Xan-thium strumarium L.). Natural Science and Discovery, 3(3), 39–43.
Eren, A. (2018). Phytoremediation of copper contaminated soil using Xanthium strumarium L. plant. Türkiye Tarımsal Araştırmalar Dergisi, 5(2), 152–157.
Glatthaar-Saalmüller, B., Rauchhaus, U., Rode, S., Haunschild, J., & Saalmüller, A. (2011). Antiviral activity in vitro of two preparations of the herbal medicinal product Sinupret® against viruses causing respiratory infections. Phytomedicine, 19(1), 1–7.
Huband, M. D., Pfaller, M. A., Shortridge, D., & Flamm, R. K. (2019). Surveillance of omadacycline activity tested against clinical isolates from the United States and Europe: Results from the SENTRY Antimicrobial Surveillance Pro-gramme, 2017. Journal of Global Antimicrobial Resistance, 19, 56–63.
Kamaraj, C., Ragavendran, C., Kumar, R. C. S., Ali, A., Khan, S. U., Mashwani, Z. U. R., Luna-Arias, J. P., & Pedroza, J. P. R. (2022). Antiparasitic potential of as-teraceae plants: A comprehensive review on therapeutic and mechanistic as-pects for biocompatible drug discovery. Phytomedicine Plus, 2(4), 100377.
Kamboj, A., & Saluja, A. (2010). Phytopharmacological review of Xanthium stru-marium L. (Cocklebur). International Journal of Green Pharmacy, 4(3), 129.
Keya, S. N., Shahed-Al-Mahmud, M., & Rahman, M. (2018). Antidepressant-like effects of methanolic extract of Xanthium strumarium (Asteraceae) in mice. Current Trends in Biotechnology and Pharmacy, 12(1), 33–37.
Kore, P. S., Kalaskar, S., Jachak, D., Jadhav, S., Patil, D., Patil, S., & Kuchekar, M. (2023). Anticataleptic effect of Xanthium strumarium hydroalcoholic extract in laboratory animals. International Journal of Biology, Pharmacy and Allied Sciences, 12(8), 3617–3627.
Kozuharova, E., Ionkova, I., & Spadaro, V. (2019). Xanthium strumarium – a potential cheap resource of plant substances for medicinal use. Flora Mediterranea, 29, 93–102.
Krstić, J., Malidža, G., Rajković, M., & Krstić, M. (2021). Influence of different methods for breaking seed dormancy of Ambrosia artemisiifolia, Abutilon theophrasti and Xanthium strumarium on emergence in field conditions. Acta Herbologica, 30(1), 29–37.
März, R. W., Ismail, C., & Popp, M. A. (1999). Profile and effectiveness of a phyto-genic combination preparation for treatment of sinusitis. Wiener Medizinische Wochenschrift, 149(8–10), 202–208.
Nasir, F., & Khan, M. S. (2012). Validation of some of the ethnopharmacological uses of Xanthium strumarium and Duchesnea indica. Pakistan Journal of Bota-ny, 44(4), 1199–1201.
Nibret, E., Youns, M., Krauth-Siegel, R. L., & Wink, M. (2011). Biological activities of xanthatin from Xanthium strumarium leaves. Phytotherapy Research, 25(12), 1883–1890.
Ozturk, Y., Keskin, Z., Tanyildizi, S., Gul Baykalir, B., Korkak, F. A., Dagoglu Hark, B., & Dagoglu, G. (2021). Atractyloside levels in Xanthium strumarium and atractyloside concentrations in the serum of rats given Xanthium strumarium. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27(5), 589–594.
Palchykov, V. A., Zazharskyi, V. V., Brygadyrenko, V. V., Davydenko, P. O., Kulishenko, O. M., & Borovik, I. V. (2020). Chemical composition and anti-bacterial effect of ethanolic extract of Buxus sempervirens on cryogenic strains of microorganisms in vitro. Chemical Data Collections, 25, 100323.
Pfaller, M. A., Huband, M. D., Shortridge, D., & Flamm, R. K. (2021). Surveillance of omadacycline activity tested against clinical isolates from the USA: Report from the SENTRY Antimicrobial Surveillance Program, 2019. Journal of Global Antimicrobial Resistance, 27, 337–351.
Plumlee, K. H. (2004). Clinical veterinary toxicology. Mosby, Saint Luis.
Sahoo, R. K., Tamuli, K. J., Lhouvum, N., Dutta, D., Bordoloi, M., Sharma, H. K., Gogoi, K., & Bhattacharyya, D. R. (2020). Phytochemical constituents from Xanthium strumarium L. and evaluation of their in vitro antimalarial activities. South African Journal of Botany, 135, 35–40.
Sahu, R., Gahlot, K., Patra, A., & Jososhi, R. (2010). Pharmacognostical standardization of leaves of Xanthium strumarium Linn. Pharmacognosy Journal, 2(12), 492–497.
Salisbury, F. B. (2019). Xanthium strumarium. In: Halevy, A. H. (Ed.). CRC hand-book of flowering. CRC Press, Boca Raton. Vol. 4. Pp. 473–522.
Shahed-Al-Mahmud, M., & Lina, S. M. M. (2017). Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice. Clinical Phytoscience, 3(1), 20.
Shravani, V. P., Sundari, S. K., Jeyachandran, S., & Nagesh, S. (2023). Green syn-thesis and characterization of Xanthium strumarium-mediated titanium dioxide nanoparticles. Cureus, 15(12), e51012.
Simonsen, G. S. (2018). Antimicrobial resistance surveillance in Europe and beyond. Eurosurveillance, 23, 42.
Tendencia, E. A. (2004). Disk diffusion method. In: Laboratory manual of standar-dized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center. Pp. 13–29.
Van Wyk, J. A., & Mayhew, E. (2013). Morphological identifcation of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide. Onderstepoort Journal of Veterinary Research, 80(1), a539.
Van Wyk, J., Cabaret, J., & Michael, L. (2004). Morphological identification of nematode larvae of small ruminants and cattle simplified. Veterinary Parasitology, 119(4), 277–306.
Zajac, A. M., & Conboy, G. A. (2011). Veterinary clinical parasitology. 8th ed. Willey-Blackwell, New York.
Zarifikhosroshahi, M., & Ergun, Z. (2022). The effect of maturation status on fatty acid profile of Xanthium strumarium L. fruits oil. Osmaniye Korkut Ata Üni-versitesi Fen Bilimleri Enstitüsü Dergisi, 5(2), 998–1007.
Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2019). Antimicrobial activity of 50 plant extracts. Biosystems Diversity, 27(2), 163–169.
Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2020a). Antibacterial and fungicidal activities of ethanol extracts from Cotinus coggygria, Rhus typhina, R. trilobata, Toxicodendron orientale, Hedera helix, Aralia elata, Leptopus chinensis and Mahonia aquifolium. Regulatory Mechanisms in Biosystems, 11(2), 305–309.
Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., Kabar, A. M., & Brygadyrenko, V. V. (2020b). Antibacterial and fungicidal effect of ethanol extracts from Juniperus sabina, Chamaecyparis lawsoniana, Pseudot-suga menziesii and Cephalotaxus harringtonia. Regulatory Mechanisms in Biosystems, 11(1), 105–109.
Zheng, L., Chen, F., Li, Y., & Li, C. (2022). Identification of a unique germacrene a oxidase from Xanthium strumarium. Phyton, 91(5), 989–998.
Azimova, S. S., & Glushenkova, A. I. (2012). Xanthium strumarium L. In: Azimova, S. S., & Glushenkova, A. I. (Eds.). Lipids, lipophilic components and essential oils from plant sources. Springer Nature. P. 143.
Bengtsson-Palme, J., Angelin, M., Huss, M., Kjellqvist, S., Kristiansson, E., Palmgren, H., Larsson, D. G. J., & Johansson, A. (2015). The human gut mi-crobiome as a transporter of antibiotic resistance genes between continents. Antimicrobial Agents and Chemotherapy, 59(10), 6551–6560.
Boyko, A. A., & Brygadyrenko, V. V. (2016). Influence of water infusion of medi-cinal plants on larvae of Strongyloides papillosus (Nematoda, Strongyloididae). Visnyk of Dnipropetrovsk University, Biology, Ecology, 24(2), 519–525.
Boyko, O. O., & Brygadyrenko, V. V. (2019). Nematocidial activity of aqueous solutions of plants of the families Cupressaceae, Rosaceae, Asteraceae, Faba-ceae, Cannabaceae and Apiaceae. Biosystems Diversity, 27(3), 227–232.
Boyko, O. O., Kabar, A. M., & Brygadyrenko, V. V. (2020). Nematicidal activity of aqueous tinctures of medicinal plants against larvae of the nematodes Strongy-loides papillosus and Haemonchus contortus. Biosystems Diversity, 28(1), 119–123.
Boyko, O., Shendryk, L., Shaban, O., & Brygadyrenko, V. (2021). Influence of essential oils on sporulation of Eimeria magna oocysts. Annals of Parasitology, 67(1), 11–17.
Cesur, C., Coşge Şenkal, B., & Uskutoğlu, T. (2022). Fatty acid composition of seed oil from cocklebur (Xantium strumarium subsp. strumarium) grown in Turkey. Cumhuriyet Science Journal, 43(3), 379–383.
Chavan, S. T., & Kulkarni, A. U. (2021). Morphological and phytochemical studies on Xanthium strumarium L. Plantae Scientia, 4(6), 287–290.
Devkota, A., & Das, R. K. (2018). Antibacterial activities of Xanthium strumarium L. Journal of Natural History Museum, 29, 70–77.
Doğan, G., & Kıran, Y. (2017). Karyotype analysis of common cocklebur (Xan-thium strumarium L.). Natural Science and Discovery, 3(3), 39–43.
Eren, A. (2018). Phytoremediation of copper contaminated soil using Xanthium strumarium L. plant. Türkiye Tarımsal Araştırmalar Dergisi, 5(2), 152–157.
Glatthaar-Saalmüller, B., Rauchhaus, U., Rode, S., Haunschild, J., & Saalmüller, A. (2011). Antiviral activity in vitro of two preparations of the herbal medicinal product Sinupret® against viruses causing respiratory infections. Phytomedicine, 19(1), 1–7.
Huband, M. D., Pfaller, M. A., Shortridge, D., & Flamm, R. K. (2019). Surveillance of omadacycline activity tested against clinical isolates from the United States and Europe: Results from the SENTRY Antimicrobial Surveillance Pro-gramme, 2017. Journal of Global Antimicrobial Resistance, 19, 56–63.
Kamaraj, C., Ragavendran, C., Kumar, R. C. S., Ali, A., Khan, S. U., Mashwani, Z. U. R., Luna-Arias, J. P., & Pedroza, J. P. R. (2022). Antiparasitic potential of as-teraceae plants: A comprehensive review on therapeutic and mechanistic as-pects for biocompatible drug discovery. Phytomedicine Plus, 2(4), 100377.
Kamboj, A., & Saluja, A. (2010). Phytopharmacological review of Xanthium stru-marium L. (Cocklebur). International Journal of Green Pharmacy, 4(3), 129.
Keya, S. N., Shahed-Al-Mahmud, M., & Rahman, M. (2018). Antidepressant-like effects of methanolic extract of Xanthium strumarium (Asteraceae) in mice. Current Trends in Biotechnology and Pharmacy, 12(1), 33–37.
Kore, P. S., Kalaskar, S., Jachak, D., Jadhav, S., Patil, D., Patil, S., & Kuchekar, M. (2023). Anticataleptic effect of Xanthium strumarium hydroalcoholic extract in laboratory animals. International Journal of Biology, Pharmacy and Allied Sciences, 12(8), 3617–3627.
Kozuharova, E., Ionkova, I., & Spadaro, V. (2019). Xanthium strumarium – a potential cheap resource of plant substances for medicinal use. Flora Mediterranea, 29, 93–102.
Krstić, J., Malidža, G., Rajković, M., & Krstić, M. (2021). Influence of different methods for breaking seed dormancy of Ambrosia artemisiifolia, Abutilon theophrasti and Xanthium strumarium on emergence in field conditions. Acta Herbologica, 30(1), 29–37.
März, R. W., Ismail, C., & Popp, M. A. (1999). Profile and effectiveness of a phyto-genic combination preparation for treatment of sinusitis. Wiener Medizinische Wochenschrift, 149(8–10), 202–208.
Nasir, F., & Khan, M. S. (2012). Validation of some of the ethnopharmacological uses of Xanthium strumarium and Duchesnea indica. Pakistan Journal of Bota-ny, 44(4), 1199–1201.
Nibret, E., Youns, M., Krauth-Siegel, R. L., & Wink, M. (2011). Biological activities of xanthatin from Xanthium strumarium leaves. Phytotherapy Research, 25(12), 1883–1890.
Ozturk, Y., Keskin, Z., Tanyildizi, S., Gul Baykalir, B., Korkak, F. A., Dagoglu Hark, B., & Dagoglu, G. (2021). Atractyloside levels in Xanthium strumarium and atractyloside concentrations in the serum of rats given Xanthium strumarium. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27(5), 589–594.
Palchykov, V. A., Zazharskyi, V. V., Brygadyrenko, V. V., Davydenko, P. O., Kulishenko, O. M., & Borovik, I. V. (2020). Chemical composition and anti-bacterial effect of ethanolic extract of Buxus sempervirens on cryogenic strains of microorganisms in vitro. Chemical Data Collections, 25, 100323.
Pfaller, M. A., Huband, M. D., Shortridge, D., & Flamm, R. K. (2021). Surveillance of omadacycline activity tested against clinical isolates from the USA: Report from the SENTRY Antimicrobial Surveillance Program, 2019. Journal of Global Antimicrobial Resistance, 27, 337–351.
Plumlee, K. H. (2004). Clinical veterinary toxicology. Mosby, Saint Luis.
Sahoo, R. K., Tamuli, K. J., Lhouvum, N., Dutta, D., Bordoloi, M., Sharma, H. K., Gogoi, K., & Bhattacharyya, D. R. (2020). Phytochemical constituents from Xanthium strumarium L. and evaluation of their in vitro antimalarial activities. South African Journal of Botany, 135, 35–40.
Sahu, R., Gahlot, K., Patra, A., & Jososhi, R. (2010). Pharmacognostical standardization of leaves of Xanthium strumarium Linn. Pharmacognosy Journal, 2(12), 492–497.
Salisbury, F. B. (2019). Xanthium strumarium. In: Halevy, A. H. (Ed.). CRC hand-book of flowering. CRC Press, Boca Raton. Vol. 4. Pp. 473–522.
Shahed-Al-Mahmud, M., & Lina, S. M. M. (2017). Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice. Clinical Phytoscience, 3(1), 20.
Shravani, V. P., Sundari, S. K., Jeyachandran, S., & Nagesh, S. (2023). Green syn-thesis and characterization of Xanthium strumarium-mediated titanium dioxide nanoparticles. Cureus, 15(12), e51012.
Simonsen, G. S. (2018). Antimicrobial resistance surveillance in Europe and beyond. Eurosurveillance, 23, 42.
Tendencia, E. A. (2004). Disk diffusion method. In: Laboratory manual of standar-dized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center. Pp. 13–29.
Van Wyk, J. A., & Mayhew, E. (2013). Morphological identifcation of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide. Onderstepoort Journal of Veterinary Research, 80(1), a539.
Van Wyk, J., Cabaret, J., & Michael, L. (2004). Morphological identification of nematode larvae of small ruminants and cattle simplified. Veterinary Parasitology, 119(4), 277–306.
Zajac, A. M., & Conboy, G. A. (2011). Veterinary clinical parasitology. 8th ed. Willey-Blackwell, New York.
Zarifikhosroshahi, M., & Ergun, Z. (2022). The effect of maturation status on fatty acid profile of Xanthium strumarium L. fruits oil. Osmaniye Korkut Ata Üni-versitesi Fen Bilimleri Enstitüsü Dergisi, 5(2), 998–1007.
Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2019). Antimicrobial activity of 50 plant extracts. Biosystems Diversity, 27(2), 163–169.
Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2020a). Antibacterial and fungicidal activities of ethanol extracts from Cotinus coggygria, Rhus typhina, R. trilobata, Toxicodendron orientale, Hedera helix, Aralia elata, Leptopus chinensis and Mahonia aquifolium. Regulatory Mechanisms in Biosystems, 11(2), 305–309.
Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., Kabar, A. M., & Brygadyrenko, V. V. (2020b). Antibacterial and fungicidal effect of ethanol extracts from Juniperus sabina, Chamaecyparis lawsoniana, Pseudot-suga menziesii and Cephalotaxus harringtonia. Regulatory Mechanisms in Biosystems, 11(1), 105–109.
Zheng, L., Chen, F., Li, Y., & Li, C. (2022). Identification of a unique germacrene a oxidase from Xanthium strumarium. Phyton, 91(5), 989–998.
Published
2024-02-23
How to Cite
Zazharskyi, V. V., Brygadyrenko, V. V., Boyko, O. O., Bilan, M. V., & Zazharska, N. M. (2024). Antibacterial and anthelmintic activities of Xanthium strumarium (Asteraceae) extracts . Regulatory Mechanisms in Biosystems, 15(1), 129-133. https://doi.org/10.15421/022419
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