Influence of temperature on sporulation of Eimeria arloingi and Eimeria perforans oocysts

  • O. O. Boyko Dnipro State Agrarian and Economic University
  • L. I. Shendryk Dnipro State Agrarian and Economic University
  • V. S. Rudyk Dnipro State Agrarian and Economic University
  • I. A. Volovyk Dnipro State Agrarian and Economic University
  • I. A. Biben Dnipro State Agrarian and Economic University
  • V. V. Brygadyrenko Oles Honchar Dnipro National University
Keywords: sporulation; oocysts; eimeriosis; oocysts; sporozoites; sporocysts; sheep and goats; rabbits


Eimeriosis of farm animals is one of the most widespread parasitic diseases in the world. In the conditions of the steppe zone of Ukraine Eimeria perforans is more common in rabbits and E. arloingi in sheep and goats. Study of factors which influence the development of these protists on the soil surface is one of the major challenges for veterinarians working for large livestock companies and fighting against eimeriosis. Environmental temperature is able to change the speed of sporulation of oocysts Eimeria. Five values of temperature (15, 20, 25, 30, and 35 °C) were used in a laboratory experiment in vitro. At 15 °C, the process of sporulation of E. arloingi and E. perforans in 100% of cases ended on the eighth day. With increase in temperature to 35 °C, the duration of sporulation decreased to three days. When the temperature was 30 °C the completion of sporulation was observed in more than 50% of the oocysts of E. arloingi on the second day, and for E. perforans on the third day. High-speed sporulation at elevated temperatures under conditions of global warming can result in the increasing spread of eimeriosis among wild and domestic animals.


Andrushko, E. S., & Egorov, S. V. (2015). Epizootological monitoring of eimeriosis in young cattle in agricultural farms of Ivanovo region and contiguous areas. Russian Journal of Parasitology, 2, 27–31.

Arfan Zaman, M., Iqbal, Z., Abbas, R. Z., & Ehtisham-ul-Haque, S. (2015). In vitro efficacy of herbal extracts against Eimeria tenella. International Journal of Agriculture and Biology, 17(4), 848–850.

Bogach, M. V., Bogach, T. V., Bezdetko, L. J., & Bondarenko, L. V. (2015). Ocinka efektyvnosti Toldu i Bajkoksu ta jih vplyv na biohimichni pokaznyky syrovatky krovi ovec za ejmerioznoji invaziji [Evaluation of the effectiveness of Tolcoccid and Baikoks and their effect on the biochemical parameters of sheep serum in Eimeria invasion]. Bulletin of Sumy National Agrarian University, Series of Veterinary Medicine, 7, 143–146 (in Ukrainian).

Boyko, O., Shendryk, L., Shaban, O., & Brygadyrenko, V. (2021). Influence of essential oils on sporulation of Eimeria magna oocysts. Annals of Parasitology, 67(1), 11–17.

Byrka, V. I., Mazannyj, O. V., & Nikiforova, O. V. (2017). Ejmeriozno-tryhurozna invazija ovec (poshyrennja, projav ta likuvannja) [Eimeriosis-trichurosis invasion of sheep (spread, onset and treatment)]. Problems of Zooengineering and Veterinary Medicine, 34(2), 282–287 (in Ukrainian).

Dovgij, J. J., Kushnirova, G. A., & Dovgij, M. J. (2013). Ejmeriozy hutrovyh zviriv i domashnih ptahiv [Eimeriosis of fur animals and poultry]. Veterynarna Medycyna, 97, 378–379 (in Ukrainian).

Fatemi, A., Razavi, S. M., Asasi, K., & Torabi Goudarzi, M. (2015). Effects of Artemisia annua extracts on sporulation of Eimeria oocysts. Parasitology Research, 114(3), 1207–1211.

Fatoba, A. J., & Adeleke, M. A. (2018). Diagnosis and control of chicken coccidiosis: A recent update. Journal of Parasitic Diseases, 42(4), 483–493.

Franchuk, L. O. (2015). Ejmerioz kroliv (poshyrennja, patogenez, likuvannja) [Eimeriosis of rabbits (spread, pathogenesis, treatment)]. Nacionalnyj Universytet Bioresursiv i Pryrodokorystuvannja Ukrajiny, Kyiv (in Ukrainian).

Gadelhaq, S. M., Arafa, W. M., & Abolhadid, S. M. (2018). In vitro activity of natural and chemical products on sporulation of Eimeria species oocysts of chickens. Veterinary Parasitology, 251, 12–16.

Girkovyj, A. J. (2012). Invazovanist’ kurej zbudnykamy ejmeriozu v gospodarstvah L’vivs’koji oblasti [Invasion of chickens by eimeriosis pathogens in farms of L’viv region]. Naukovo-Tehnichnyj Bjuleten Instytutu Biologiji Tvaryn ta Derzhavnogo Naukovo-Doslidnogo Kontrol’nogo Instytutu Vetpreparativ ta Kormovyh Dobavok, 13, 198–200 (in Ukrainian).

Graat, E. A. M., Henken, A. M., Ploeger, H. W., Noordhuizen, J. P. T. M., & Vertommen, M. H. (1994). Rate and course of sporulation of oocyst of Eimeria acervulina under different environmental conditions. Parasitology, 108, 497–502.

Gutyj, B., Stybel, V., Hariv, I., Maksymovych, I., Buczek, K., Staniec, M., Milczak, A., Bushueva, I., Kulish, S., Shcherbyna, R., & Samura, T. (2019). Influence of amprolinsile and brovitacoccid on the protein synthesizing function of the liver and enzyme activity in turkey blood serum during Eimeria invasion. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 10(2), 723–729.

Kachanova, E. O., & Pavlova, E. V. (2020). Efficacy of the coadministration of coccidiocide and prebiotic to broiler chickens infected with Eimeria tenella oocysts. IOP Conference Series: Earth and Environmental Science, 548, 042009.

Ketema, E., & Fasil, N. (2019). Study on prevalence and associated risk factors of poultry coccidiosis in and around alage at vet college, Southwestern Ethiopia. Journal of Dairy and Veterinary Sciences, 11(1), 555805.

Klymenko, O. S. (2015). Poshyrennja parazytoziv kroliv u pryvatnyh gospodarstvah Poltavs’koji oblasti [Distribution of rabbit parasitosis in private farms of Poltava region]. Visnyk Poltavs’koji Derzhavnoji Agrarnoji Akademiji, 1–2, 109–112 (in Ukrainian).

Korjachkov, V. A. (2015). Ejmerioz kroliv ta nutrij (poshyrennja, diagnostyka ta zahody borot’by) [Eimeriosis of rabbits and coypu (distribution, diagnosis and control measures)]. Lvivs’kyj Nacional’nyj Universytet Veterynarnoji Medycyny ta Biotehnologij imeni S. Z. Gzhyckogo, Lviv (in Ukrainian).

Makaida, M. V., Pakhomov, O. Y., & Brygadyrenko, V. V. (2021). Effect of increased ambient temperature on seasonal generation number in Lucilia sericata (Diptera, Calliphoridae). Folia Oecologica, 48(2), 191–198.

Marquardt, W. C. (1960). Effect of high temperature on sporulation of Eimeria zurnii. Experimental Parasitology, 10(1), 58–65.

Marquardt, W. C., Senger, C. M., & Seghetti, L. (1960). The effect of physical and chemical agents on the oocyst of Eimeria zurnii (Protozoa, Coccidia). The Journal of Protozoology, 7(2), 186–189.

Müller, J., & Hemphill, A. (2013). In vitro culture systems for the study of apicomplexan parasites in farm animals. International Journal for Parasitology, 43(2), 115–124.

Prus, M. P., Duda, J. V., & Korejba, L. V. (2021). Invazovanist’ ejmeriozom kroliv u domogospodarstvah Ukrajiny zalezhno vid pryrodno-klimatychnoji zony [Invasion with eimeriosis of rabbits in Ukrainian households depending on the natural and climatic zone]. In: Klimatychni Zminy ta Sil’s’ke Gospodarstvo, Vyklyky dlia Agrarnoji Nauky ta Osvity. VFPO, Kyiv. Pp. 26–30 (in Ukrainian).

Pyziel, A. M., & Demiaszkiewicz, A. W. (2015). Observations on sporulation of Eimeria bovis (Apicomplexa: Eimeriidae) from the European bison Bison bonasus: Effect of temperature and potassium dichromate solution. Folia Parasitologica, 62(1), 20.

Ruiz, A., González, J. F., Rodríguez, E., Martín, S., Hernández, Y. I., Almeida, R., & Molina, J. M. (2006). Influence of climatic and management factors on Eimeria infections in goats from semi-arid zones. Journal of Veterinary Medicine Series B, 53(8), 399–402.

Sazmand, A., Hamidinejat, H., Hekmatimoghaddam, S., Asadollahi, Z., & Mirabdollahi, S. (2012). Eimeria infection in camels (Camelus dromedarius) in Yazd province, Central Iran. Tropical Biomedicine, 29(1), 77–80.

Schneiders, G. H., Foutz, J. C., Fuller, A. L., Nelson, J., Rekaya, R., & Aggrey, S. E. (2020). The effect of increased temperatures on viability, morphology, infectivity, and development of Eimeria tenella. Journal of Parasitology, 106(3), 428.

Skalchuk, V. V. (2021). Ejmeriozno-kryptosporydiozna invazija teljat (poshyrennja, patogenez, likuvannja) [Eimeriosis-cryptosporidiosis of calves (spread, pathogenesis, treatment)]. Nacional’nyj Universytet Bioresursiv i Pryrodokorystuvannja Ukrajiny, Kyiv (in Ukrainian).

Soliman, E. S., Sallam, N. H., & Abouelhassan, E. M. (2018). Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation. Veterinary World, 11(8), 1064–1073.

Tadesse, C., & Feyissa, B. D. (2016). Poultry coccidiosis: Prevalence and associated risk factors in extensive and intensive farming systems in Jimma Town, Jimma, Ethiopia. Journal of Veterinary Medicine and Animal Health, 8(12), 223–227.

Taylor, M. A., Catchpole, J., Marshall, J., Marshall, R. N., & Hoeben, D. (2003). Histopathological observations on the activity of diclazuril (Vecoxan®) against the endogenous stages of Eimeria crandallis in sheep. Veterinary Parasitology, 116(4), 305–314.

Trofimov, M. M., Onishhenko, N. G., & Pasunkina, M. O. (2013). Epizootologichni osoblyvosti ejmeriozu kroliv u gospodarstvah AR Krym [Epizootological features of rabbit eimeriosis in farms of the Autonomous Republic of Crimea]. Veterynarna Medycyna, 97, 407–409 (in Ukrainian).

Waldenstedt, L., Elwinger, K., Lundén, A., Thebo, P., & Uggla, A. (2001). Sporulation of Eimeria maxima oocysts in litter with different moisture contents. Poultry Science, 80(10), 1412–1415.

Wondimu, A., Mesfin, E., & Bayu, Y. (2019). Prevalence of poultry coccidiosis and associated risk factors in intensive farming system of Gondar Town, Ethiopia. Veterinary Medicine International, 2019(9), 1–6.

Yakovleva, I. N., Yakovleva, E. G., Dronov, V. V., & Topuria, L. Y. (2021). Efficiency evaluation of anticoccidials against the background of chickens infection with field coccidia isolate. IOP Conference Series: Earth and Environmental Science, 677(5), 052071.

Yevstafieva, V. O., Kruchynenko, O. V., Mel’nychuk, V. V., Myhajljutenko, S. M., & Korchan, L. M. (2020). Osoblyvosti poshyrennja parazytoziv ovec u osinn’o-pasovyshhnyj period [Features of the spread of sheep parasites in the autumn-grazing period]. Visnyk Poltavs’koji Derzhavnoji Agrarnoji Akademiji, 4, 163–169 (in Ukrainian).

How to Cite
Boyko, O. O., Shendryk, L. I., Rudyk, V. S., Volovyk, I. A., Biben, I. A., & Brygadyrenko, V. V. (2021). Influence of temperature on sporulation of Eimeria arloingi and Eimeria perforans oocysts . Regulatory Mechanisms in Biosystems, 12(2), 369-373.

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