X-ray densitometric indices of proximal phalanx, medial phalanx and ungular pelvic limb bones as criteria for age diagnosis of cattle in forensic veterinary expertise

  • I. V. Yatsenko Kharkiv State Animal Veterinary Academy
  • S. A. Tkachuk National University of Life and Environmental Sciences of Ukraine
  • L. V. Busol Kharkiv State Animal Veterinary Academy
  • M. M. Bondarevsky Kharkiv State Animal Veterinary Academy
  • I. V. Zabarna Podolsky State Agrarian University of Technology
  • I. A. Biben Dnipro State Agrarian and Economic University
Keywords: mathematical model; metatarsus; fingers; relative optical density; osteology.

Abstract

Morphological parameters of biological material are extremely informative in diagnostic studies, in particular, to determine the species, sex, time of death, the term of burial. The most informative object for these tasks is the skeleton, because changes in the bones are stored for a long time, while soft tissue is subjected to rotting. Bone tissue is the most durable, but at the same time, it is very labile and reacts to all metabolic processes in the body. The object of the study was proximal phalanx, medial phalanx and ungular bone of the pelvic limb of cattle ranging in age from newborn to 12 years old. Radiography of the proximal phalanx, medial phalanx and ungular bones of the pelvic limb was performed on the Arman apparatus. The bones were subjected to X-ray in the lateromedial projection. The inner and outer sections of the tubular bone were determined. The mathematical modeling of the interaction of X-rays and the cortical layer of bones of fingers (proximal phalanx, medial phalanx and ungular) of cattle was carried out in this work. It is established that this process is described by Bouger's law. The physico-mathematical model of proximal phalanx, medial phalanx and ungular bones has been calculated, on the basis of which it was possible to calculate the X-ray densitometric indices of these bones of cattle. The age features of dynamics of X-ray densitometric indices of the proximal phalanx, medial phalanx and ungular bones were established and a method of determining the age of cattle according to this criterion was proposed. A mathematical model for the proximal phalanx, medial phalanx and ungular bones of the pelvic limbs of cattle that can be applied in X-ray densitometry uses: for the average third proximal phalanx – section of heterogeneous tubular structure modeled by a semicircle; for a medial phalanx bone – a section of a triangular shape; for the ungular bone – a heterogeneous structure, the plantar surface is inscribed in a rectangle. The process of interaction of X-rays with the bone structure of the examined pelvic limb bones can be described by Bouguer's law. The developed mathematical modeling of this interaction and the algorithm for its analysis is the basis for determining the age of cattle for X-ray densitometric indices of the proximal phalanx, medial phalanx and ungular bones of pelvic limbs. By X-ray densitometry of the proximal phalanx and medial phalanx bones of the pelvic limbs extremities one can diagnose the age of bovine animals from birth to 5 years, but according to ungular bones – from birth to 10 years. X-ray densitometry of medial phalanx and ungular bones of pelvic limbs can be used for diagnosing bovine cattle in a complex with other morphological, chemical and physical methods of investigation.

References

Abuznaid, K. R. S. (2016). Zastosuvannia KT-densytometriji v doslidzhenni morfolohichnykh parametriv velykoji rohatoji khudoby [Application of KT-densitometry in the study of morphological parameters of cattle]. Radioelektronika ta modeli XXI stolittia: Materialy XX yuvileinoho mizhnarodnoho molodizhnoho forumu. Vol. 1, 88–90 (in Ukrainian).

Abuznaid, K. R. S. (2016). Znachennia morfolohichnykh parametriv kistok skeleta dlia vyrishennia diahnostychnykh zavdan suduvo-veterynarnoji ekspertyzy [The value of the morphological parameters of skeleton bone for solving diagnostic problems in the forensic-veterinary inspection]. Problemy Zootekhniji ta Veterynarnoji Medytsyny, 29, 228–249 (in Ukrainian).

Amadasi, A., Borgonovo, S., Brandone, A., Mauro, A., Di Giancamillo, M., & Cattaneo, C. (2014). A comparison between digital radiography, computed tomography and magnetic resonance in the detection of gunshot residues in burnt tissues and bone. Journal of Forensic Sciences, 59(3), 712–717.

Bondarevskyi, M. M., Yatsenko, I. V., & Averianova, L. O. (2012). Renthenodensytometrychni pokaznyky kistok plesna, yak kryteriji diahnostyky viku velykoji rohatoji khudoby u sudovij veterynarnij medytsyni [X-ray densitometric indices of metatarsal bones as a criterion for diagnosing cattle's age in forensic-veterinary inspection]. Visnyk Zhytomyrskoho Derzhavnoho Ahroekolohichnoho Universytetu, 32, 227–231 (in Ukrainian).

Chung, C., Chen, Y.-P., Leu, T.-H., & Sun, C.-W. (2018). Near-infrared bone densitometry: A feasibility study on distal radius measurement. Journal of Biophotonics, 11(7), e201700342.

Coates, D. B., Dixon, R. M., Murray, R. M., Mayer, R. J., & Miller, C. P. (2016). Bone mineral density in the tail-bones of cattle: Effect of dietary phosphorus status, liveweight, age and physiological status. Animal Production Science, 58(5), 801–810.

Davies, H. M. S. (2010). Dorsal metacarpal cortex ultrasound speed and bone size and shape. Equine Veterinary Journal, 34, 337–339.

Dicken, A. J., Evans, J. P. O., Rogers, K. D., Stone, N., Greenwood, С., Godber, S. X., Prokopiou, D., Clement, J. G., Lyburn, I. D., Martin, R. M., & Zioupos, P. (2015). X-ray diffraction from bone employing annular and semi-annular beams. Physiology Medicine Biology, 60, 5803–5812.

Girdis, C. M., Baldock, P. A., & Downes, M. (2015). Vitamin D, muscle and bone: Integraring effects in development, aging and injury. Molecular and Endocrinolody, 410, 3–10.

Jimenez-Mendoza, D., Espinosa-Arbelaez, D. G., Giraldo-Betancur, A. L., Hernandez-Urbiola, M. I., Vargas-Vazquez, D., & Rodriguez-Garcia, M. E. (2011). Single X-ray transmission system for bone mineral density determination. Review of Scientific Instruments, 82(12), 105–125.

Kamianskyi, V. V. (2010). Makroosteoskopichnyi analiz deiakykh kistok paltsiv dlia vyznachennia viku velykoi rohatoi khudoby u sudovo-veterynarnij ekspertyzi [Macroosteoscopic analysis of some bones of the finger for determining the age of cattle in the forensic-veterinary inspection]. Visnyk Poltavskoi Derzhavnoi Ahrarnoji Akademiji, 1, 214–218 (in Ukrainian).

Keene, B. E., Knowlton, K. F., McGilliard, M. L., Lawrence, L. A., Nickols-Richardson, S. M., Wilson, J. H., Rutledge, A. M., McDowell, L. R., & Van Amburgh, M. E. (2004). Measures of bone mineral content in mature dairy cows. Journal of Dairy Science, 87(1), 3816–3825.

McClure, S. R., Glickman, L. T., Glickman, N. W., & Weaver, C. M. (2001). Evaluation of dual energy X-ray absorptiometry for in situ measurement of bone mineral density of equine metacarpi. American Journal of Veterinary Research, 62(5), 752–756.

Monfared, A. L. (2013). Gross anatomical measurements of the head region of the iranian native cattle (Bos taurus) and their clinical value for regional anesthesia. Global Veterinaria, 10(2), 219–222.

Mostafa, E. М., El-Elemi, А. Н., El-Beblawy, М. А., & Dawood, A. E.-W. A. (2012). Adult sex identification using digital radiographs of the proximal epiphysis of the femur. Suez Canal University Hospital in Ismailia, Egyptian Journal Forensic Science, 2(3), 81–88.

Shanbhogue, V. V., Hansen, S., Folkestad, L., Brixen, K., &Beck-Nielsen, S. S. (2015). Bone geometry, volumetric density, microarchitecture and estimated bone strength assessed by HR-pQCT in adult patients with hypophosphatemic rickets. Journal of Bone and Mineral Research, 30(1), 176–183.

Sidoroff, V. H., Ylinen, M. K., Kröger, L. M., Kröger, H. P. J., &Korppi, M. O. (2013). Inhaled corticosteroids and bone mineral density at school age: A follow-up study after early childhood wheezing. Pediatric Pulmonology, 50(1), 1–7.

Stynder, D. D. (2008). The impact of medium-sized canids on a seal bone assemblage from Dunefield Midden, West Coast, South Africa. The South African Archaeological Bulletin, 63, 159–163.

Suster, D., Leury, B. J., Ostrowska, E., Butler, K. L., Kerton, D. J., Wark, J. D., & Dunshea, F. R. (2013). Accuracy of dual energy X-ray absorptiometry (DXA), weight and P2 back fat to predict whole body and carcass composition in pigs within and across experiment. Journal of Dairy Science, 84(3), 231–242.

Tkachuk, S. A., & Stoliarchuk, L. V. (2011). Vyznachennia vydovoji nalezhnosti miasa velykoji rohatoji khudoby vymiriuvanniam mikrotverdosti trubchastykh kistok [Determination of the species belonging to cattle meat by measuring the microhardness of tubular bones]. Naukovi Dopovidi of National University of Life and Environmental Sciences of Ukraine, 29, 1–7.

Turner, C. H. (2006.) Bone strength: Current concepts. Skeletal Development and Remodeling in Health, Disease and Aging, 1068, 429–446.

Yatsenko, I. V., Bondarevskyi, M. M., & Kamianskyi, V. V. (2013). Vikova renthenostrukturna kharakterystyka kistok plesna velykoji rohatoji khudoby v aspekti sudovoji veterynarnoji ekspertyzy [X-ray structural characteristic of cattle bones in the aspect of forensic-veterinary inspection]. Problemy Zooinzhenerii ta Veterynarnoi Medytsyny, 26(2), 274–280 (in Ukrainian).

Zotti, A., Gianesela, M., Ceccato, C., & Morgante, M. (2009). Physiological values and factors affecting the metacarpal bone density of healthy feedlot beef cattle as measured by dualenergy X-ray absorptiometry. Journal of Animal Physiology and Animal Nutrition, 94(5), 615–622.

Zotti, A., Gianesela, M., Ceccato, C., & Morgante, M. (2009). Physiological values and factors affecting the metacarpal bone density of healthy feedlot beef cattle as measured by dualenergy X-ray absorptiometry. Journal of Animal Physiology and Animal Nutrition, 94(5), 615–622.

Zotti, A., Poggi, R., & Cozzi, B. (2010). Exceptional bone density DXA values of the rostrum of a deep-diving marine mammal: A new technical insight in the adaptation of bone to aquatic life. Skeletal Radiology, 38, 1123–1125.

Zotti, A., Poggi, R., & Cozzi, B. (2010). Exceptional bone density DXA values of the rostrum of a deep-diving marine mammal: A new technical insight in the adaptation of bone to aquatic life. Skeletal Radiology, 38, 1123–1125.

Published
2019-05-09
How to Cite
Yatsenko, I. V., Tkachuk, S. A., Busol, L. V., Bondarevsky, M. M., Zabarna, I. V., & Biben, I. A. (2019). X-ray densitometric indices of proximal phalanx, medial phalanx and ungular pelvic limb bones as criteria for age diagnosis of cattle in forensic veterinary expertise . Regulatory Mechanisms in Biosystems, 10(2), 197-202. https://doi.org/10.15421/021929

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