Сontent of 1,2,4-triazole derivatives in cow's milk, meat samples and animal organs after administration

  • V. V. Parchenko Zaporizhzhia State Medical and Pharmaceutical University
  • O. I. Panasenko Zaporizhzhia State Medical and Pharmaceutical University
  • Y. V. Karpenko Zaporizhzhia State Medical and Pharmaceutical University
  • B. P. Kyrychko Poltava State Agrarian Academy
  • T. V. Zvenihorodska Poltava State Agrarian Academy
  • O. A. Bigdan Zaporizhzhia State Medical and Pharmaceutical University
  • T. O. Samura Zaporizhzhia State Medical and Pharmaceutical University
  • N. O. Nahorna Zaporizhzhia State Medical and Pharmaceutical University
  • Y. O. Mykhailiuk Zaporizhzhia State Medical and Pharmaceutical University
  • D. M. Romanina Zaporizhzhia State Medical and Pharmaceutical University
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • V. P. Martynyshyn Lviv University of Business and Law
DOI: https://doi.org/10.15421/0224119
Keywords: liquid chromatography-mass spectrometry; 1,2,4-triazole; Trifuzol-neo.

Abstract

Derivatives of 1,2,4-triazoles are widely used as active pharmaceutical ingredients (APIs) in medical preparations. The work deals with the drug “Trifuzol-neo”, the active substance of which belongs to water-soluble derivatives of 1,2,4-triazole. The aim of our work was to assess the applicability of the HPLC-MS method and determine residues of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio)acetate in cow milk, meat samples and animal organs after administration. The content of the medicinal substance in the cow’s milk sample was measured 24 hours after the injection of the 1% solution “Trifuzol-neo”. Active pharmaceutical ingredients concentration in the tested milk solution was 0.0695 µg/g. It was shown that 12 hours after the intramuscular injection, the active substance was not detected in the liver and spleen. 24 hours after the injection, the concentration was 0.1045 μg/g, 48 hours after the injection, the active substance was detected, but its concentration did not exceed 0.1 ppm. The developed HPLC-MS based technique is efficient and capable of being used in pharmaceutical, toxico-chemical, veterinary and food laboratories. The methodologies may be realized either in food control, or in food and veterinary investigation.

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Published
2024-10-10
How to Cite
Parchenko, V. V., Panasenko, O. I., Karpenko, Y. V., Kyrychko, B. P., Zvenihorodska, T. V., Bigdan, O. A., Samura, T. O., Nahorna, N. O., Mykhailiuk, Y. O., Romanina, D. M., Gutyj, B. V., & Martynyshyn, V. P. (2024). Сontent of 1,2,4-triazole derivatives in cow’s milk, meat samples and animal organs after administration . Regulatory Mechanisms in Biosystems, 15(4), 830-836. https://doi.org/10.15421/0224119
Issue
Vol 15 No 4 (2024): Regulatory Mechanisms in Biosystems
Section
Статті
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