Effect of consumption of I, Se, S and nanoaquacitrates on hematological and biochemical parameters of the organism of rabbits

  • О. V. Boiko Сherkassy Experimental Station of Bioresources National Academy of Agricultural Sciences of Ukraine
  • О. F. Honchar Сherkassy Experimental Station of Bioresources National Academy of Agricultural Sciences of Ukraine
  • Y. V. Lesyk Institute of Animal Biology of NAAS
  • І. І. Kovalchuk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • A. Z. Dychok-Niedzielska Institute of Animal Biology of NAAS
Keywords: nanotechnologies; mineral substances; formed elements of blood; enzymes; protein


Using nanotechnologies to obtain compounds of mineral substances is promising because of the importance of the amount of mineral substances, but the limiting factor is particularly their bioavailability in the digestive tract. The conducted studies determined the stimulating effect of nanoaquachelates of separate biogene microelements on the course of biochemical processes of the organisms of animals, their productivity and quality of the obtained products. However, the mechanisms of impact of carboxylates of separate elements and their combinations on functioning of physiological systems of various species of animals, level of transformation of these elements into the products and their biological value remain unstudied. Therefore, the objectives of the study were changes in hematological biochemical and productive parameters of the organisms of rabbits consuming I, Se, S, the impacts of these elements on their organism from the 53 to 85th days of life. Studies were performed on young Termonde rabbits. Animals of experimental groups I, II, III, and IV were fed with feeds of the diet of the control group for 24 h with water containing solution of І, Se, S calculated respectively as 2.5, 5.0, 10.0 and 20.0 µg of І/L of water. The experiment lasted 45 days, including a 12 day preparation period and 33 day experimental. On the 53rd day of life of animals in the preparation period, and 68th and 85th days of life in the experimental periods (15th and 33rd days of watering with supplements), we collected samples of blood from the marginal ear vein of 6 animals (3 males and 3 females) of the group for hematological and biochemical studies. We determined that watering with the solution of microelements І, Se, S led to changes in the overall amount of white blood cells, making it 17.1% lower in the blood of animals of the experimental group II, 26.2% higher in group ІІІ on the 15th day, and 12.0% higher in group IV on the 31st day of the experiment compared with the control. Absolute amounts of lymphocytes, monocytes and granulocytes by the periods of the study were observed to have no significant changes throughout the study, though their parameters were within the physiological values compared with the control. The amount of red blood cells in the blood of rabbits of experimental groups І, ІІ and ІІІ was higher respectively by 8.1%, 5.7% and 12.0% at the first stage of the study and 18.1%, 13.3%, 24.5% and 24.9% higher in animals of experimental groups І, ІІ; ІІІ and ІV at the final stage of the study compared with the control group. Hemoglobin concentration in blood of rabbits of experimental groups II and III during the study was significantly higher. Hematocrit value in blood of rabbits of experimental groups I–III was significantly higher on the 33rd day of the experiment compared with the control. Consumption of solution of microelements in the amount of 10.0 µg of I/L by the animals of experimental group III led to 15.1% higher content of total protein in the blood on the 33rd day of the study compared with the control group of animals. The level of creatinine was no higher than the physiological parameters and was 9.2%, 15.0% and 15.4% higher in the blood of rabbits of experimental groups II, III, IV on the 33rd day of the experiment compared with the control group. No significant changes in the content of triacylglycerols in the blood of rabbits of the experimental groups were seen throughout the study. However, the content of cholesterol in the blood of rabbits of experimental groups III and IV was significantly higher at the first stage compared with the control group. Further, it is practical to study physiologically substantiated amounts of solution of microelements І, Se, S in the diet of mother rabbits to determine their effect on their ability to become pregnant, reproductive function, milk production and survival of offspring.


Abd El-Hack, M. E., Abdelnour, S. A., Taha, A. E., Khafaga, A. F., Arif, M., Ayasan, T., Swelum, A. A., Abukhalil, M. H., Alkahtani, S., & Aleya, L. (2020). Herbs as thermoregulatory agents in poultry: An overview. Science of the Total Environment, 703, 134–399.

Abd-Allah, S., & Hashem, K. S. (2015). Selenium nanoparticles increase the testicular antioxidant activity and spermatogenesis in male rats as compared to ordinary selenium. Journal of Analytical Science and Technology, 3(1), 792–802.

Abdel-Wareth, A. A. A., & Metwally, A. E. (2020). Productive and physiological response of male rabbits to dietary supplementation with thyme essential oil. Animals, 10(10), 1844.

Abdel-Wareth, A. A. A., Taha, E. M., Südekum, K.-H., & Lohakare, J. (2018). Thyme oil inclusion levels in a rabbit ration: Evaluation of productive performance, carcass criteria and meat quality under hot environmental conditions. Animal Nutrition, 4(4), 410–416.

Abouelezz, K. F. M., Abou-Hadied, M., Yuan, J., Elokil, A. A., Wang, G., Wang, S., Wang, J., & Bian, G. (2019). Nutritional impacts of dietary oregano and Enviva essential oils on the performance, gut microbiota and blood biochemicals of growing ducks. Animal, 13, 2216–2222.

Boostani, A., Sadeghi, A., Mousavi, S., Chamani, M., & Kashan, N. (2015). Effects of organic, inorganic, and nano-Se on growth performance, antioxidant capacity, cellular and humoral immune responses in broiler chickens exposed to oxidative stress. Livestock Science, 178, 330–336.

Darmohray, L. M., Luchyn, I. S., Gutyj, B. V., Golovach, P. I., Zhelavskyi, M. M., Paskevych, G. A., & Vishchur, V. Y. (2019). Trace elements transformation in young rabbit muscles. Ukrainian Journal of Ecology, 9(4), 616–621.

Dwyer, J. T., Wiemer, K. L., Dary, O., Keen, C. L., King, J. C., Miller, K. B., Philbert, M. A., Tarasuk, V., Taylor, C. L., & Gaine, P. C. (2015). Fortification and health: Challenges and opportunities. Advances in Nutrition, 6(1), 124–131.

Fawzy, M. M., El-sadawi, H. A., & El-dien, M. H. (2016). Hematological and biochemical performance of poultry following zinc oxide and sodium selenite supplementation as food additives. Annals of Clinical Pathology, 4(4), 1076.

Glazer, E. S., Zhu, C., Hamir, A. N., Borne, A., Thompson, C. S., & Curley, S. A. (2011). Biodistribution and acute toxicity of naked gold nanoparticles in a rabbit hepatic tumor model. Nanotoxicology, 5(4), 459–468.

Kerry, R. G., Patra, J. K., Gouda, S., Park, Y., Shin, H. S., & Das, G. (2018). Benefaction of probiotics for human health: A review. Journal of Food and Drug Analysis, 26(3), 927–939.

Kisera, Y. V., Storchak, Y. G., Gutyj, B. V., Bozhyk, L. Y., Magrelo, N., Sus, Y., Dashkovskyy, O., Pryimych, V. I., Vus, U., Kit, L., & Sachuk, R. (2019). Structural and functional features of the vermiform appendix at the tissue and cellular levels in rabbits after the introduction of immunobiological drugs. Ukrainian Journal of Ecology, 9(2), 217–226.

Kozik, V., Bak, A., Pentak, D., Hachula, B., Pytlakowska, K., Rojkiewicz, M., Jampilek, J., Sieron, K., Jazowiecka-Rakus, J., & Sochanik, A. (2019). Derivatives of graphene oxide as potential drug carriers. Journal of Nanoscience and Nanotechnology, 19(5), 2489–2492.

Lesyk, Y., Ivanytska, A., Kovalchuk, I., Monastyrska, S., Hoivanovych, N., Gutyj, B., Zhelavskyi, M., Hulai, O., Midyk, S., Yakubchak, O., & Poltavchenko, T. (2020). Hematological parameters and content of lipids in tissues of the organism of rabbit saccording to the silic on connection. Ukrainian Journal of Ecology, 10(1), 15–22.

Reda, F. M., El-Saadony, M. T., Elnesr, S. S., Alagawany, M., & Tufarelli, V. (2020). Effect of dietary supplementation of biological curcumin nanoparticles on growth and carcass traits, antioxidant status, immunity and caecal microbiota of Japanese quails. Animals, 10(5), 754.

Samak, D. H., El-Sayed, Y. S., Shaheen, H., El-Far, A., Swelum, A. A., Noreldin, A., El-Naggar, K., Abdelnour, S., Saied, E. M., El-Seedi, H. R. (2018). Developmental toxicity of carbon nanoparticles during embryogenesis in chicken. Environmental Science and Pollution Research, 27, 19058–19072.

Sheiha, A. M., Abdelnour, S. A., El-Hack, M. E. A., Khafaga, A. F., Metwally, K. A., Ajarem, J. S., Maodaa, S. N., Allam, A. A., & El-Saadony, M. T. (2020). Effects of dietary biological or chemical-synthesized nano-selenium supplementation on growing rabbits exposed to thermal stress. Animal, 10(3), 430.

Shi, L. G., Xun, W. J., Yue, W. B., Zhang, C. X., Ren, Y. S., Liu, Q., Wang, Q., & Shi, L. (2011). Effect of sodium selenite, Se-yeast and nano-elemental selenium on growth performance, Se concentration and antioxidant status in growing male goats. Small Ruminant Research, 96(1), 49–52.

Shulman, M. V., Pakhomov, O. Y., & Brygadyrenko, V. V. (2017). Effect of lead and cadmium ions upon the pupariation and morphological changes in Calliphora vicina (Diptera, Calliphoridae). Folia Oecologica, 44(1), 28–37.

Sobolev, O. I., Gutyj, B. V., Sobolievа, S. V., Borshch, О. O., Kushnir, I. M., Petryshak, R. A., Naumyuk, O. S., Kushnir, V. I., Petryshak, O. Y., Zhelavskyi, M. M., Todoriuk, V. B., Sus, H. V., Levkivska, N. D., Vysotskij, A. O., & Magrelo, N. V. (2020). Review of germanium environmental distribution, migrateon and accumulation. Ukrainian Journal of Ecology, 10(2), 200–208.

Sobolev, O. I., Gutyj, B. V., Sobolievа, S. V., Borshch, О. O., Nedashkivsky, V. M., Kachan, L. M., Karkach, P. M., Nedashkivska, N. V., Poroshinska, O. А., Stovbetska, L. S., Emelyanenko, A. A., Shmayun, S. S., & Guta, Z. A. (2020). Selenium in natural environment and food chains. A review. Ukrainian Journal of Ecology, 10(4), 148–158.

Sobolev, O. I., Lisohurska, D. V., Pyvovar, P. V., Topolnytskyi, P. Р., Gutyj, B. V., Sobolievа, S. V., Borshch, О. O., Liskovich, V. А., Verkholiuk, M. M., Petryszak, O. Y., Kuliaba, O. V., Golodiuk, I. P., Naumjuk, O. S., Petryszak, R. A., & Dutka, H. I. (2021). Modeling the effect of different dose of selenium additives in compound feed on the efficiency of broiler chicken growth. Ukrainian Journal of Ecology, 11(2), 292–299.

Swelum, A. A., Elbestawy, A. R., El-Saadony, M. T., Hussein, E. O. S., Alhotan, R., Suliman, G. M., Taha, A. E., Ba-Awadh, H., El-Tarabily, K. A., & Abd El-Hack, M. E. (2021). Ways to minimize bacterial infections, with special reference to Escherichia coli, to cope with the first-week mortality in chicks: An updated overview. Poultry Science, 100(5), 101–139.

Vlislo, V. V. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnij medytsyni [Laboratory methods of research in biology, animal husbandry and veterinary medicine]. Spolom, Lviv (in Ukrainian).

Zazharska, N., Boyko, O., & Brygadyrenko, V. (2018). Influence of diet on the productivity and characteristics of goat milk. Indian Journal of Animal Research, 52(5), 711–717.

Zheng, S., Zhao, J., Xing, H., & Xu, S. (2019). Oxidative stress, inflammation, and glycometabolism disorder-induced erythrocyte hemolysis in selenium‐deficient exudative diathesis broilers. Journal of Cellular Physiology, 234(9), 16328–16337.

How to Cite
BoikoО. V., HoncharО. F., Lesyk, Y. V., KovalchukІ. І., Gutyj, B. V., & Dychok-Niedzielska, A. Z. (2021). Effect of consumption of I, Se, S and nanoaquacitrates on hematological and biochemical parameters of the organism of rabbits . Regulatory Mechanisms in Biosystems, 12(2), 335-340. https://doi.org/10.15421/022145

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