Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis

  • A. R. Shcherbatyy Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • L. G. Slivinska Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • V. L. Fedorovych Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • B. O. Lukashchuk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
Keywords: lipid hydroperoxides; malonic dialdehyde; immunity; bactericidal activity; blood serum; circulating immune complexes.

Abstract

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.

References

Bajpai, V. K., Sharma, A., Kang, S. C., & Baek, K. H. (2014). Antioxidant, lipid peroxidation inhibition and free radical scavenging efficacy of a diterpenoid compound sugiol isolated from Metasequoia glyptostroboides. Asian Pacific Journal of Tropical Medicine, 7(1), 9–15.

Bomko, V., Kropyvka, Y., Bomko, L., Chernyuk, S., Kropyvka, S., & Gutyj, B. (2018). Effect of mixed ligand complexes of zinc, manganese, and cobalt on the manganese balance in high-yielding cows during first 100-days lactation. Ukrainian Journal of Ecology, 8(1), 420–425.

Clarke, A. F., Madelin, T. M., & Allpress, R. G. (1987). The relationship of air hygiene in stables to lower airway disease and pharyngeal lymphoid hyper plasia in two groups of thoroughbred horses. Equine Veterinary Journal, 19(6), 524–530.

Droge, W. (2002). Free radical in the physiological control of cell function. Phy siological Reviews, 82(1), 47–95.

Esterbauer, H., Gebicki, J., Puhl, H., & Jürgens, G. (1992). The role of lipid per oxidation and antioxidants in oxidative modification of LDL. Free Radical Biology and Medicine, 13(4), 341–390.

Fielding, C. L., Magdesian, K. G., Rhodes, D. M., Meier, C. A., & Higgins, J. C. (2009). Clinical and biochemical abnormalities in endurance horses eliminated from competition for medical complications and requiring emergency medi cal treatment: 30 cases (2005–2006). Journal of Veterinary Emergency and Critical Care, 19(5), 473–478.

Gerber, V., Tessier, C., & Marti, E. (2014). Genetics of upper and lower airway diseases in the horse. Equine Veterinary Journal, 47(4), 390–397.

Golovach, M. J., Ganynec, P. P., & Golovach, M. M. (2005). Vykorystannja konej gucul's'koi' porody ta i'h pomisej dlja riznyh form gipoterapii' [Use of hutsul breed horses and their breeding grounds for various forms of hippotherapy]. Silskyj Gospodar, 7, 16–18 (in Ukrainian).

Gutyj, B., Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., Fedorovych, O., Rusyn, V., & Kolomiiets, I. (2017). The influence of Metisevit on bioche mical and morphological indicators of blood of piglets under nitrate loading. Regulatory Mechanisms in Biosystems, 8(3), 427–432.

Gutyj, B., Stybel, V., Darmohray, L., Lavryshyn, Y., Turko, I., Hachak, Y., Shcher batyy, A., Bushueva, I., Parchenko, V., Kaplaushenko, A., & Krushelnytska, O. (2017). Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load. Ukrainian Journal of Ecology, 7(4), 589–596.

Holovakha, V. I., Piddubnуak, О. V., Bakhur, T. I., Vovkotrub, N. V., Antipov, A. A., Anfiorova, M. V., Gutyj, B. V., Slivinska, L. G., Kurdeko, O. P., & Macyno vich, A. O. (2018). Changes in erythrocytopoesis indices in dogs with babe siosis. Regulatory Mechanisms in Biosystems, 9(3), 379–383.

Hutyi, B. V., Murska, S. D., Hufrii, D. F., Khariv, I. I., Levkivska, N. D., Nazaruk, N. V., Haidiuk, M. B., Pryima, O. B., Bilyk, O. I., & Huta, Z. A. (2016). Vplyv kadmiievoho navantazhennia na systemu antyoksydantnoho zakhystu orhanizmu buhaitsiv [Influence of cadmium loading on the state of the antioxidant system in the organism of bulls]. Visnyk Dnipropetrovsk University, Biology, Ecology, 24(1), 96–102 (in Ukrainian).

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541.

Korabeinykova, S. N. (1989). Modyfykatsyia opredelenyia POL v reaktsyy s TBK [Modification of the definition of POL in the reaction with TBA]. Laborator noe Delo, 7, 8–9 (in Russian).

Kuljaba, O. V., Stybel, V. V., & Gutyj, B. V. (2016). The influence of clozaverm A and catozal on antioxidant status of cows’ organism for the experimental fasciolosis, sensitized atypical mycobacteria. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, 66, 96–99.

Lavryshyn, Y. Y., Varkholyak, I. S., Martyschuk, T. V., Guta, Z. А., Ivankiv, L. B., Paladischuk, О. R., Murska, S. D., Gutyj, B. V., & Gufriy, D. F. (2016). The biological significance of the antioxidant defense system of animals’ body. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, 66, 100–111.

Maksymovych, I. A. (2017). Exchange of electrolytes in sports horse for exercise. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, 77, 100–104.

Maksymovych, I. А., & Slivinskа, L. G. (2018). Metabolic responses in endurance horses at exhausted syndrome. Ukrainian Journal of Veterinary and Agricul tural Sciences, 1(1), 17–22.

Mironchik, V. V. (1984). Sposob opredeleniya gidroperekisey lipidov v biologi cheskikh tkanyakh [Method for determination of lipid hydroperoxides in biological tissues]. Avtorskoye svidetel'stvo No 1084681 SSSR, MKI G No 33/48 (SSSR). No 3468369/2813; zayavl. 08.07.82; opubl. 07.04.84. Byul. No 13 (in Russian).

Niki, E. (2009). Lipid peroxidation: Physiological levels and dual biological ef fects. Free Radical Biology and Medicine, 47(5), 469–484.

Padalino, B., Raidal, S., Carter, N., Celi, P., Muscatello, G., Jeffcott, L., & de Silva, K. (2017). Immunological, clinical, haematological and oxidative responses to long distance transportation in horses. Research in Veterinary Science, 115, 78–87.

Shcherbatyy, A. G., Slivinska, L. G., Gutyj, B. V., Golovakha, V. I., Piddubnyak, A. V., & Fedorovuch, V. L. (2017). The influence of a mineral-vitamin premix on the metabolism of pregnant horses with microelemetosis. Regulatory Mechanisms in Biosystems, 8(2), 293–398.

Shherbatyj, A. R., Slivins’ka, L. G. (2013). Diagnostyka mikroelementoziv kobyl u zahidnij biogeohimichnij zoni Ukrajiny [Diagnosis of microelementosis of mares in the western biogeochemical zone of Ukraine]. Veterynarna Medycyna Ukrajiny, 206, 25‒28 (in Ukrainian).

Slivinska, L., Shcherbatyy, A., Gutyj, B., Lychuk, M., Fedorovych, V., Maksy movych, I., Rusyn, V., & Chernushkin, B. (2018). Parameters of erythrocyto poiesis, acid resistance and population composition of erythrocytes of cows with chronic hematuria. Ukrainian Journal of Ecology, 8(1), 379–385.

Sobolev, O., Gutyj, B., Petryshak, R., Pivtorak, J., Kovalskyi, Y., Naumyuk, A., Petryshak, O., Semchuk, I., Mateusz, V., Shcherbatyy, A., & Semeniv, B. (2018). Biological role of selenium in the organism of animals and humans. Ukrainian Journal of Ecology, 8(1), 654–665.

Stalnaia, Y. D. (1977). Sovremennye metody v biokhimii [Modern methods in biochemistry]. Meditsina, Moscow (in Russian).

Vishchur, O. I., Hutyi, B. V., Hufrii, D. F., & Khariv, I. I. (2015). Imunnyi status, sposoby otsinky i metody korektsii u teliat rannoho viku [Immune status, assessment methods and methods of correction in young calves]. Spolom, Lviv (in Ukrainian).

Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory methods up to bio logy, animal husbandry and veterinary medicine]. Spolom, Lviv (in Ukrainian).

Wingfield, N. J. (1984). Horse ailments and health care: A practical horse guide Colin Vogel. Equine Veterinary Journal, 16(1), 52.

Published
2019-02-26
How to Cite
Shcherbatyy, A. R., Slivinska, L. G., Gutyj, B. V., Fedorovych, V. L., & Lukashchuk, B. O. (2019). Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis . Regulatory Mechanisms in Biosystems, 10(1), 87-91. https://doi.org/10.15421/021914

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