Three-dimensional structure of the lingual papillae of healthy rats and rats with experimental diabetes mellitus (in the context of mechanism of development of diabetic glossitis)
Abstract
We studied the three-dimensional structure and patterns of distribution of the lingual papillae of healthy rats (the norm) and their changes in the process of development of diabetes mellitus І type. The research was conducted on 65 laboratory rats of the Weestar line. The research investigated the mucus shell and the microcirculatory network of the tongue. The distribution and three-dimensional structure of the papillae of the tongue were studied using a scanning electron microscope. It was found that there are 5 morphological subspecies of filiform papillae on the dorsal surface of body of the tongue: true filifom, flattened, thin and giant conical and brush-like. Isolated fungiform papillaе are unevenly distributed between filiform papillaе. The dorso-lateral edge of the dorsal lingual surface is covered by foliate papillae. The unique oval papilla vallate is located in the back-end of the middle line of the root of the tongue. The far back of the root of the tongue lacks papillae, is flattened and covered by squamous formations. The distribution and types of lingual papillae is similar in rats to other rodents. In the process of development of diabetic glossitis a reduction in the height of different types of papillae of the tongue was observed, and an increase in the amount of keratinized mass, which plays a role in the fixation of microflora on the surface of the mucus shell, which as a result may lead to development of inflammatory process in the tongues of rats with experimental diabetes mellitus. The stages of morphological and morphometric changes in the mucus shell and microcirculatory network of the tongues of rats with diabetes mellitus were investigated, the characteristic signs of these changes were marked. On the basis of morpho-functional changes of the tongues of rats with experimental streptozotocin induced diabetes mellitus, two stages of development of pathomorphological changes were distinguished: 1) reactive changes (2–4th week) and 2) destructive processes (6–8th week). At the end of the first stage there was a reduction in height of the filiform papillae and width of mushroom-like papillae in the mucus shell of the tongue, an increase in its keratinization, a considerable reduction in the number of cells in the deeper layers of the epithelium of the tongue and the adsorption capacity of superficial epіtheliocites diminished, a significant reduction in the diameter of path clearance of all departments of the microcirculatory network is traced here. At the end of the secondary stage, there was a reduction in the sizes of all papillae of the back of the tongue, in all links of the microcirculatory network there was a development of diabetic microangiopathy which is characterized: by narrowing of the arterial and exchange links on a background expansion of capacity link. The question of influencing the pathological process in the vessels of the microcirculatory network on the state of the mucus shell of the tongue in animals with experimental streptozotocin induced diabetes mellitus is discussed.References
Abdel-Reheim, E. S., Abd-Elmoneim A. A., & Hosni, A. A. (2014). Fatty-sucrosed diet/minimal dose of streptozotocin-treated rat: A novel model of gestational diabetes mellitus, metabolic and inflammatory insight. Journal of Diabetes and Metabolism, 5(9), 430–435. >> doi.org/10.4172/2155-6156.1000430
Abumandour, M. M. A. (2014). Morphological comparison of the filiform papillae of New Zealand white rabbits (Oryctolagus cuniculus) as domestic mammals and Egyptian fruit bat (Rousettus aegyptiacus) as wild mammals using scanning electron microscopic specimens. International Journal of Morphology, 32, 1407–1417. >> doi.org/10.4067/s0717-95022014000400045
Benetti, E. J., Pícoli, L. C., Guimarães, J. P., Motoyama, A. A., Miglino, M. A., & Watanabe, L.-S. (2009) Characteristics of filiform, fungiform and vallate papillae and surface of interface epithelium-connective tissue of the maned sloth tongue mucosa (Bradypus torquatus, Iliger, 1811): Light and scanning electron microscopy study. Anatomia, Histologia, Embryologia, 38, 42–48. >> doi.org/10.1111/j.1439-0264.2008.00890.x
Can, M., Atalgin, H., Ates, S., & Takci, L. (2016). Scanning electron microscopic study on the structure of the lingual papillae of the Karacabey Merino sheep. Eurasian Journal of Veterinary Sciences, 32, 130–130. >> doi.org/10.15312/eurasianjvetsci.2016318389
Ding, Y., Yu, S., & Shao, B. (2016). Anatomical and histological characteristic of the tongue and tongue mucosa linguae in the cattle-yak (Bos taurus × Bos grunniens). Frontiers in Biology, 11(2), 141–148. >> doi.org/10.1007/s11515-016-1393-3
Doran, G. A. (1995). Review of the evolution and phylogeny of the mamma¬lian tongue. Acta Anatomica, 91, 118–129. >> doi.org/10.1159/000144377
El-Bakry, A. M. (2009). Study by transmission and scanning electron microscopy of the morphogenesis of three types of lingual papillae in the albino rat (Rattus rattus). Zoologica (Stockholm), 91, 267–278. >> doi.org/10.1111/j.1463-6395.2009.00406.x
Emura, S. A., Tamada, D., & Hayakawa, D. (1999). SEM study on the dor¬sal lingual surface of Microtus montebelli. Okajimas Folia Anatomica Japonica, 76, 171–177. >> doi.org/10.2535/ofaj1936.76.4_171
Emura, S., Hayakawa, D., Chen, H., & Shoumura, S. (2002). Morphology of the dorsal lingual papillae in the Japanese macaque and Savanna monkey. Anatomia, Histologia, Embryologia, 31, 313–316. >> doi.org/10.1046/j.1439-0264.2002.00378.x
Emura, S., Tamada, A., & Hayakawa, D. (2000). Morphology of the dorsal lingual papillae in the black rhinoceros (Diceros bicornis). Anatomia, Histologia, Embryologia, 29, 371–374. >> doi.org/10.1046/j.1439-0264.2000.00283.x
Emura, S., Tamada, A., & Hayakawa, D. (2001). SEM study on the dorsal lingual surface of the nutria Myocastor coypus. Acta Anatomica Nipponica, 76, 233–238. >> doi.org/10.2535/ofaj1936.77.5_137
Emura, S., Tamada, A., & Hayakawa, D. (2002). SEM Study on the dorsal lingual surface of the large flying fox, Pteropus vampyrus. Okajimas Folia Anatomica Japonica, 79(4), 113–119. >> doi.org/10.2535/ofaj.79.113
Goodarzi, N., & Hoseini, T. S. (2015). Fine structure of lingual papillae in the markhoz goat (Iranian angora): A scanning electron microscopic study. International Journal of Zoological Research, 11, 160–168. >> doi.org/10.3923/ijzr.2015.160.168
Gouri, A., Dekaken, A., Rouabhia, S., Bentorki, A. A., & Yakhlef, A. (2013). Transaminases profile in Algerian patients with type 2 diabetes mellitus. Immuno-Analyse and Biologie Spéciali, 28(1), 25–29. >> doi.org/10.1016/j.immbio.2012.07.021
Iwasaki, S. (2002). Evolution of the structure and function of the vertebrate tongue. Journal of Anatomy, 201, 1–13. >> doi.org/10.1046/j.1469-7580.2002.00073.x
Jackowiak, H., & Godynicki, S. (2005). Light and scanning electron microsco¬pic study of the tongue in the cow. Annals of Anatomy, 187, 251–259. >> doi.org/10.1016/j.aanat.2004.11.003
Jackowiak, H., & Godynicki, S. (2005). The distribution and structure of the lingual papillae on the tongue of the bank vole Clethrionomys glareolus. Folia Morphologica, 64(4), 326–333. >> doi.org/10.1016/s0940-9602(04)80037-2
Jackowiak, H., Skieresz-Szewczyk, K., Kwieciński, Z., Godynicki, S., Jackowiak, K., & Leszczyszyn, A. (2014). Light microscopy and scanning electron microscopy studies on the reduction of the tongue microstructures in the white stork (Ciconia ciconia, Aves). Acta Zoologica, 96, 436–441. >> doi.org/10.1111/azo.12087
Kilinc, M., Erdogan, S., Ketani, S., & Ketani, M. A. (2010). Morphological study by scanning electron microscopy of the lingual papillae in the Middle East blind mole rat. Anatomia, Histologia, Embryologia, 39, 509–515. >> doi.org/10.1111/j.1439-0264.2010.01022.x
Kulawik, M., Godynicki, S., & Frąckowiak, H. (2015). Light and scanning electron microscopic study of the filiform papillae of the tongue in adult rabbit (Oryctolagus cuniculus f. domestica, Linnaeus 1758). Nauka Przyroda Technologie, 9, 1–12. >> doi.org/10.17306/j.npt.2015.1.12
Kurtul, I., & Atalgın, S. H. (2008). Scanning electron microscopic study on the structure of the lingual papillae of the Saanen goat. Small Ruminant Research, 80, 52–56. >> doi.org/10.1016/j.smallrumres.2008.09.003
Meo, S. A. (2009). Diabetes mellitus: Health and wealth threat. International Journal of Diabetes Mellitus, 1(1), 42. >> doi.org/10.1016/j.ijdm.2009.03.007
Mistretta, C. M., & Liu, H.-X. (2006). Development of fungiform papillae: Patterned lingual gustatory organs. Archives of Histology and Cytology, 69(4), 199–208. >> doi.org/10.1679/aohc.69.199
Miyawaki, Y., Yoshimura, K., Shindo, J., & Kageyama, I., (2010). Light and scanning electron microscopic study on the tongue and lingual papillae of the common raccoon, Procyon lotor. Okajimas Folia Anatomica Japonica, 87, 65–73. >> doi.org/10.2535/ofaj.87.65
Nonaka, K., Zheng, J. H., & Kobayashi, K. (2008). Comparative morpholo¬gical study on the lingual papillae and their connective tissue cores in rabbits. Okajimas Folia Anatomica Japonica, 85, 57–66. >> doi.org/10.2535/ofaj.85.57
O’Reilly, D., & Long, R. G. (2008). Diabetes and the gastro-intestinal tract. Digestive Diseases, 5(1), 57–64. >> doi.org/10.1159/000171163
Ojima, K., Mitsuhashi, F., Nasu, M., & Suzuki, Y. (2000). Angioarchitectural form, functional distributive pattern and classification of thefiliform papil¬lae on the crossbred Japanese cat tongue anterodorsal surface in scanning electron microscopic specimens. Annals of Anatomy – Anatomischer Anzeiger, 182, 47–52. >> doi.org/10.1016/s0940-9602(00)80120-x
Okada, S., & Schraufnagel, D. E. (2005). Scanning electron microscopic structure of the lingual papillae of the common opossum (Didelphis marsupialis). Microscopy and Microanalysis, 11, 319–332. >> doi.org/10.1017/s1431927605050257
Reginato, G. de S., Bolina, C. de S., Watanabe, L., & Ciena, A. P. (2014). Three-dimensional aspects of the lingual papillae and their connective tissue cores in the tongue of rats: A scanning electron microscope study. The Scientific World Journal, 2014, 1–6. >> doi.org/10.1155/2014/841879
Rezki, A., Merioud, B., Delmas, D., Cyril, C., Scheiwiller, R., Leblé, R., & Valensi, P. (2016). Sequential compression/decompression by a pulsating suit increases cutaneous microcirculatory blood flow in patients with type 2 diabetes. Diabetes and Metabolism, 42(4), 298. >> doi.org/10.1016/j.diabet.2016.07.016
Rukavina, M. (2016). Typ-1-diabetes – verhältnis proinsulin / C-peptid mit pathogenese assoziiert. Diabetologie und Stoffwechsel, 11(6), 384–396. >> doi.org/10.1055/s-0042-121733
Scardina, G. A., & Messina, P. (2006). Microvascular characteristics of the human filiform papillae: A videocapillaroscopic study. Anatomy – Anatomischer Anzeiger, 188, 183–186. >> doi.org/10.1016/j.aanat.2005.08.016
Toprak, B., & Yilmaz, S. (2016). Light and scanning electron microscopic investigation of postnatal development of vallate papillae in the white laboratory mice. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 11, 131–137. >> doi.org/10.17094/avbd.04368
Uemura, M., Tamada, Y., & Suwa, F. (2009). Morphological study of the connective tissue papillae and the capillary loops on the lingual dorsum in the type 2 diabetes mellitus model rats. Okajimas Folia Anatomica Japonica, 85(4), 139–149. >> doi.org/10.2535/ofaj.85.139
Westfall, S., Lomis, N., Singh, S. P., Dai, S. Y., & Prakash, S. (2015). The gut microflora and its metabolites regulate the molecular crosstalk between diabetes and neurodegeneration. Journal of Diabetes and Metabolism, 6(8), 577–580. >> doi.org/10.4172/2155-6156.100057
Wołczuk, K. (2014). Dorsal surface of the tongue of the hazel dormouse muscardinus avellanarius: Scanning electron and light microscopic studies. Zoologica Poloniae, 59, 35–47. >> doi.org/10.2478/zoop-2014-0004
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