Current epizootological and epidemiological aspects of brucellosis in Ukraine

  • L. Y. Korniienko State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
  • V. V. Ukhovskyi State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
  • O. A. Moroz State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
  • O. M. Chechet State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
  • G. B. Aliekseieva State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
  • T. M. Tsarenko Bila Tserkva National Agrarian University
  • M. S. Karpulenko State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
  • N. P. Nenych Chernihiv Regional State Laboratory of the State Service of Ukraine on Food Safety and Consumer Protection
  • M. L. Radzykhovskyi National University of Life and Environmental Sciences of Ukraine
Keywords: Brucella; сattle; pigs; small ruminants; horses; wild boars; roe deer; hares; humans; serological monitoring


The analysis of monitoring studies for brucellosis among farm and wild animals for the period 2004–2021 in Ukraine was performed. The total number of tests on brucellosis during this period among cattle amounted to 62,917,946 animals, small ruminants – 10,898,075, pigs – 4,146,751, horses – 116,668, wild pigs – 22,306, roe deer – 11,548, hares (only the last two years were studied) – 430 animals. During the same period, camels, deer, dogs, cats, rabbits, poultry, zoo animals, wild animals including moose, foxes and badgers were examined in case of suspicion or request. During this period, seropositive animals were found among cattle – 607, small ruminants – 84, pigs – 219, horses – 4, dogs – 2, wild boars – 52, hares – 7. There were years when no positive animals were detected (2012, 2015), 1–3 animals were detected in 2008, 2010, 2013, 2014, 2016, 2017. The largest number of animals positive for brucellosis was detected in 2004 and 2006, respectively 107 and 328 animals. Some regions stand out among others with a significant number of positive results. Thus, in Sumy region, positive animals were isolated in 2004, 2005, 2007, 2009, 2010, 2011, 2013, 2019, 2020, 2021 (a total of 45 animals), in Dnipropetrovsk region in 2004, 2016, 2017, 2018 (a total of 11 animals). The results of the analysis showed that human brucellosis in Ukraine is registered sporadically (single cases). During the analyzed period, 32 cases of human brucellosis were detected, during the period 1994–2021, the diagnosis of brucellosis was confirmed in 45 people. Approximately 50% of human brucellosis cases in Ukraine are introduced from abroad. The vector role of wild animals-reservoirs in the spread of the brucellosis pathogen in the direction of farm animals and from them to humans is confirmed. Among the regions, the most disadvantaged were Chernihiv (cases in 2009, 2011, 2014, 2015), Lviv (2005, 2011, 2021) and Kyiv (2008, 2014, 2021). During the period 1994–2021, the disease was not registered in people at all in the Autonomous Republic of Crimea, Volyn, Vinnytsia, Transcarpathian, Zaporizhzhia, Kirovohrad, Rivne, Ternopil, Cherkasy regions.


Assenga, J. A., Matemba, L. E., Muller, S. K., Malakalinga, J. J., & Kazwala, R. R. (2015). Epidemiology of Brucella infection in the human, livestock and wildlife interface in the Katavi-Rukwa ecosystem, Tanzania. BMC Veterinary Research, 11, 189.

Bakri, F. G., AlQadiri, H. M., & Adwan, M. H. (2018). The highest cited papers in brucellosis: Identification using two databases and review of the papers’ major findings. BioMed Research International, 2018, 9291326.

Bamaiyi, P. H. (2016). Prevalence and risk factors of brucellosis in man and domestic animals: A review. International Journal of One Health, 2, 29–34.

Bolotin, V. I., Pikun, O. Y., Marchenko, N. V., Kozhevnik, I. Y., Rudova, N. G., Solodiankin, O. S., Stegniy, B. T., & Gerilovych, A. P. (2020). First report of Canine brucellosis in Ukraine: Pathogen isolation and characterization. Journal for Veterinary Medicine Biotechnology and Biosafety, 6(4), 5–8.

Bouza, E., Sánchez-Carrillo, C., Hernangómez, S., & González, M. J. (2005). Laboratory-acquired brucellosis: A Spanish national survey. The Journal of Hospital Infection, 61(1), 80–83.

Busol, V. A., Babkin, A. F., & Zhovanik, P. N. (1991). Brucellez sel’skohozjajstvennyh zhivotnyh [Brucellosis in farm animals]. Urozhaj, Kyiv (in Russian).

Chemych, M. D., Ilina, N. I., Ilina, V. V., Malysh, N. H., Bolotin, V. I., Obukhovska, O. V., Fotina, T. I., & Fotina, H. A. (2017). Brutseloz u suchasnykh umovakh [Brucellosis in modern conditions]. Infektsiini Khvoroby, 90, 55–61 (in Ukrainian).

Christopher, S., Umapathy, B. L., & Ravikumar, K. L. (2010). Brucellosis: Review on the recent trends in pathogenicity and laboratory diagnosis. Journal of Laboratory Physicians, 2(2), 55–60.

Covert, J., Mathison, A. J., Eskra, L., Banai, M., & Splitter, G. (2009). Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses. Experimental Biology and Medicine, 234(12), 1450–1467.

Dean, A. S., Crump, L., Greter, H., Schelling, E., & Zinsstag, J. (2012). Global burden of human brucellosis: A systematic review of disease frequency. PLoS Neglected Tropical Diseases, 6(10), e1865.

Franc, K. A., Krecek, R. C., Häsler, B. N., & Arenas-Gamboa, A. M. (2018). Brucellosis remains a neglected disease in the developing world: A call for interdisciplinary action. BMC Public Health, 18(1), 125.

Głowacka, P., Żakowska, D., Naylor, K., Niemcewicz, M., & Bielawska-Drózd, A. (2018). Brucella–virulence factors, pathogenesis and treatment. Polish Journal of Microbiology, 67(2), 151–161.

Godfroid, J., Cloeckaert, A., Liautard, J. P., Kohler, S., Fretin, D., Walravens, K., Garin-Bastuji, B., & Letesson, J. J. (2005). From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Veterinary Research, 36(3), 313–326.

Hull, N. C., & Schumaker, B. A. (2018). Comparisons of brucellosis between human and veterinary medicine. Infection Ecology and Epidemiology, 8(1), 1500846.

Johansen, M. V., Welburn, S. C., Dorny, P., & Brattig, N. W. (2017). Control of neglected zoonotic diseases. Acta Tropica, 165, 1–2.

Khan, M. Z., & Zahoor, M. (2018). An overview of brucellosis in cattle and humans, and its serological and molecular diagnosis in control strategies. Tropical Medicine and Infectious Disease, 3(2), 65.

Khurana, S. K., Sehrawat, A., Tiwari, R., Prasad, M., Gulati, B., Shabbir, M. Z., Chhabra, R., Karthik, K., Patel, S. K., Pathak, M., Iqbal Yatoo, M., Gupta, V. K., Dhama, K., Sah, R., & Chaicumpa, W. (2021). Bovine brucellosis – a comprehensive review. The Veterinary Quarterly, 41(1), 61–88.

Kneipp, C., Malik, R., Mor, S. M., & Wiethoelter, A. K. (2019). Commentary: Retrospective and prospective perspectives on zoonotic brucellosis. Frontiers in Microbiology, 10, 1859.

Ko, J., & Splitter, G. A. (2003). Molecular host-pathogen interaction in brucellosis: Current understanding and future approaches to vaccine development for mice and humans. Clinical Microbiology Reviews, 16(1), 65–78.

Korniienko, L. I., Busol, V. O., Nedosiekov, V. V., Kucheriavenko, O. O., Kucheriavenko, O. O., & Korniienko, L. M. (2009). Khronichni infektsiini khvoroby tvaryn [Chronic infectious diseases of animals]. BTSAU Publishing, Bila Tserkva (in Ukrainian).

Köse, Ş., Serin Senger, S., Akkoçlu, G., Kuzucu, L., Ulu, Y., Ersan, G., & Oğuz, F. (2014). Clinical manifestations, complications, and treatment of brucellosis: Evaluation of 72 cases. Turkish Journal of Medical Sciences, 44(2), 220–223.

Lai, S., Chen, Q., & Li, Z. (2021). Human brucellosis: An ongoing global health challenge. China CDC Weekly, 3(6), 120–123.

Lai, S., Zhou, H., Xiong, W., Gilbert, M., Huang, Z., Yu, J., Yin, W., Wang, L., Chen, Q., Li, Y., Mu, D., Zeng, L., Ren, X., Geng, M., Zhang, Z., Cui, B., Li, T., Wang, D., Li, Z., Wardrop, N. A., & Yu, H. (2017). Changing epidemiology of human brucellosis, China, 1955–2014. Emerging Infectious Diseases, 23(2), 184–194.

Li, T., Tong, Z., Huang, M., Tang, L., Zhang, H., & Chen, C. (2017). Brucella melitensis M5-90Δbp26 as a potential live vaccine that allows for the distinction between natural infection and immunization. Canadian Journal of Microbiology, 63(8), 719–729.

Lucero, N. E., Ayala, S. M., Escobar, G. I., & Jacob, N. R. (2008). Brucella isolated in humans and animals in Latin America from 1968 to 2006. Epidemiology and Infection, 136(4), 496–503.

Madkour, M. M. (2001). Madkour’s brucellosis. Springer Verlag, New York.

Markovych, I. H. (2020). Intehralnyi analiz ruinivnykh syl epidemichnoho protsesu ta vdoskonalennia systemy kontroliu nad nym [An integral analysis of the destructive forces of the epidemic process and the improvement of the system of its control]. Instytut epidemiolohii ta Infektsiinykh Khvorob im. L. V. Hromashevskoho Natsionalnoi Akademii Medychnykh Nauk Ukrainy, Kyiv (in Ukrainian).

McDermott, J., Grace, D., & Zinsstag, J. (2013). Economics of brucellosis impact and control in low-income countries. Revue Scientifique et Technique, 32(1), 249–261.

Moreno, E. (2014). Retrospective and prospective perspectives on zoonotic brucellosis. Frontiers in Microbiology, 5, 213.

Musallam, I. I., Abo-Shehada, M. N., Hegazy, Y. M., Holt, H. R., & Guitian, F. J. (2016). Systematic review of brucellosis in the Middle East: Disease frequency in ruminants and humans and risk factors for human infection. Epidemiology and Infection, 144(4), 671–685.

Nevolko, O. (2017). Monitoring of brucellosis in agricultural animals in Ukraine during 2013–2015. Online Journal Public Health Inform, 9(1), e156.

Novokhatnii, Y., Vydaiko, N., Nebohatkin, I., Bilonik, O., & Svita, V. (2017). Monitorynh za infektsiiamy spilnymy dlia liudei i tvaryn (leptospiroz, brutseloz, ku-lykhomanka, skaz, sybirka) – odna zi skladovykh zabezpechennia biobezpeky naselennia Ukrainy [Monitoring of infectious diseases of humans and animals (leptospirosis, brucellosis, q-fever, rabies, anthrax) is one of the complex issues of biosecurity of the population of Ukraine]. Veterynarna Medytsyna, 103, 58–60 (in Ukrainian).

Olsen, S. C., & Palmer, M. V. (2014). Advancement of knowledge of Brucella over the past 50 years. Veterinary Pathology, 51(6), 1076–1089.

Olsen, S. C., & Tatum, F. M. (2016). Swine brucellosis: Current perspectives. Veterinary Medicine, 8, 1–12.

Pappas, G., Panagopoulou, P., Christou, L., & Akritidis, N. (2006). Brucella as a biological weapon. Cellular and Molecular Life Sciences, 63(19–20), 2229–2236.

Pappas, G., Papadimitriou, P., Akritidis, N., Christou, L., & Tsianos, E. V. (2006). The new global map of human brucellosis. The Lancet. Infectious Diseases, 6(2), 91–99.

Pedersen, K., Quance, C. R., Robbe-Austerman, S., Piaggio, A. J., Bevins, S. N., Goldstein, S. M., Gaston, W. D., & DeLiberto, T. J. (2014). Identification of Brucella suis from feral swine in selected states in the USA. Journal of Wildlife Diseases, 50(2), 171–179.

Pyskun, A., Polishchuk, O., Piankivska, I., Pyskun, O., Moroz, O., Pishchanskyi, O., & Aliekseieva, H. (2019). Presence of antibodies against Brucella spp. in serum samples from wild boars in Ukraine. Porcine Research, 9(1), 26–33.

Roop, R. M., Barton, I. S., Hopersberger, D., & Martin, D. W. (2021). Uncovering the hidden credentials of Brucella virulence. Microbiology and Molecular Biology Reviews, 85(1), e00021–19.

Rossetti, C. A., Arenas-Gamboa, A. M., & Maurizio, E. (2017). Caprine brucellosis: A historically neglected disease with significant impact on public health. Neglected Tropical Diseases, 11(8), e0005692.

Rossetti, C. A., Maurizio, E., & Rossi, U. A. (2022). Comparative review of brucellosis in small domestic ruminants. Frontiers in Veterinary Science, 9, 887671.

Roy, S., McElwain, T. F., & Wan, Y. (2011). A network control theory approach to modeling and optimal control of zoonoses: Case study of brucellosis transmission in sub-Saharan Africa. Neglected Tropical Diseases, 5(10), e1259.

Sabra, A., el Masry, B., & Shaib, H. (2021). A review of brucellosis: A recent major outbreak in Lebanon. Journal of Environmental Science and Public Health, 5(1), 56–76.

Schumaker, B. (2013). Risks of Brucella abortus spillover in the Greater Yellowstone area. Revue Scientifique et Technique, 32(1), 71–77.

Sohn, A. H., Probert, W. S., Glaser, C. A., Gupta, N., Bollen, A. W., Wong, J. D., Grace, E. M., & McDonald, W. C. (2003). Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp. Emerging Infectious Diseases, 9(4), 485–488.

Wang, X. H., & Jiang, H. (2020). Global prevalence of human brucellosis. Zhonghua Liuxingbingxue Zazhi, 41(10), 1717–1722.

Zhang, N., Huang, D., Wu, W., Liu, J., Liang, F., Zhou, B., & Guan, P. (2018). Animal brucellosis control or eradication programs worldwide: A systematic review of experiences and lessons learned. Preventive Veterinary Medicine, 160, 105–115.

Zinsstag, J., Schelling, E., Waltner-Toews, D., & Tanner, M. (2011). From "one medicine" to "one health" and systemic approaches to health and well-being. Preventive Veterinary Medicine, 101(3–4), 148–156.

How to Cite
Korniienko, L. Y., Ukhovskyi, V. V., Moroz, O. A., Chechet, O. M., Aliekseieva, G. B., Tsarenko, T. M., Karpulenko, M. S., Nenych, N. P., & Radzykhovskyi, M. L. (2023). Current epizootological and epidemiological aspects of brucellosis in Ukraine . Regulatory Mechanisms in Biosystems, 14(1), 77-85.

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