Hematological and biochemical parameters and resistance of the organism of mother rabbits receiving sulfur compounds
AbstractNanotechnologies are developing continuously, and their use is becoming more diverse and concrete, with high potential to improve the products of animal husbandry and animals in general. Yet, there have been few studies of nanotechnologies in those spheres. However, to confirm the efficiency and, mainly, safety of nanotechnologies, to avoid any harm to the environment or especially humans and animals, substantiated studies are still needed. A promising, but barely studied, direction of research is dosage of nanocompounds of biogenic elements in diets of animals, both modern supplements and alternatives for correcting mineral nutrition. Therefore, the objectives of the study presented here were changes in hematologic, biochemical and immune-biological parameters of the organism of mother rabbits under the influence of sulfur citrate and sodium sulfate for 14 days until insemination and until day 20 of lactation. The research was conducted on mother rabbits of the Hyla breed which were giving birth for the second time in LLC Horlytsia in Dobriany village of Horodok district of Lviv Oblast. Mother rabbits of the control group were fed ad libitum with complete granulated mixed feed with free access to water. Animals of experimental group I were fed feeds of the diet of the control group and during the day were watered sulfate citrate in dosage of 8 µg of S/kg of body weight. Females of experimental group II were fed the diet feeds of the control group and given sodium sulfate (Na2SO4) in the amount of 40 mg of S/kg of body weight. The mother rabbits received the supplements for 14 days both until insemination and for up to the 20th day of lactation. In the preparation period, on day 10 after the beginning of the study and on day 20 of lactation in the experimental period (65th day of watering the supplements), blood samples were taken from the marginal ear vein of the mother rabbits for hematological and biochemical studies. The study revealed that watering sulfate citrate in the dosage of 8 µg of S/kg of the body weight in the diet of mother rabbits for 14 days until insemination and up to day 20 of lactation increased the amount of erythrocytes by 19.5%, leukocytes by 37.5%, granulocytes by 38.3%, concentration of hemoglobin by 21.0%, mean corpuscular hemoglobin by 15.6%, and red cell distribution width by 14.7%, and activated protein metabolism, which manifested in 8.5% higher content of protein and activity of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase respectively by 12.9%, 29.6%, 19.8% and 51.8% lower content of triacylglycerols in the blood on day 20 of lactation compared with the control group. Intake of sulfur citrate heightened the parameters of immunobiological reactivity of the organisms of mother rabbits on day 65 of the experiment with significantly higher level of phagocytic number, phagocytic activity, lysozymic and bactericidal activities of blood serum, protein-associated hexoses, sialic acids and ceruloplasmin and immune globulins, compared with the control. Giving mother rabbits sodium sulfate in the amount of 40 mg/kg of body weight for 65 days led to less notable changes in the blood, expressed in 20.0% increase in mean corpuscular hemoglobin, higher phagocytic value, phagocytic activity, lysozymic and bactericidal activities of blood serum, protein-associated hexoses and ceruloplasmin. The results of the studies indicate possibility of additional use of supplement sulfur citrate in the amount of 8 µg of S/kg in diet of mother rabbits to improve metabolism, reproductive ability and immunological resistance in the period of increased physiological load.
Abouelezz, K. F. M., Abou-Hadied, M., Yuan, J., Elokil, A. A., Wang, G., Wang, S., Wang, J., & Bian, G. (2019). Nutritional impacts of dietary oregano and Enviva essential oils on the performance, gut microbiota and blood biochemicals of growing ducks. Animal, 13(10), 2216–2222.
Alagón, G., Arce, O. N., Martínez-Paredes, E. M., Ródenas, L., Moya, V. J., Blas, E., Cervera, C., & Pascual, J. J. (2016). Nutritive value of distillers dried grains with solubles from barley, corn and wheat for growing rabbits. Animal Feed Science and Technology, 222, 217–226.
Bashchenko, M. I., Boiko, O. V., Honchar, O. F., Sotnichenko, Y. M., Tkach, Y. F., Gavrysh, O. M., Nebylytsja, M. S., Lesyk, Y. V., & Gutyj, B. V. (2021). The cow’s calving in the selection of bull-breeder in monbeliard, norwegian red and holstine breed. Ukrainian Journal of Ecology, 11(2), 236–240.
Bojko, O. V., Darmohray, L. M., Luchyn, I. S., Honchar, O. F., & Gutyj, B. V. (2020). Specific activity of Sr-90 and Cs-137 in rabbits of various genotypes. Ukrainian Journal of Ecology, 10(2), 165–169.
Borshch, O. O., Ruban, S. Y., Gutyj, B. V., Borshch, O. V., Sobolev, O. I., Kosior, L. T., Fedorchenko, M. M., Kirii, A. A., Pivtorak, Y. I., Salamakha, I. Y., Hordiichuk, N. M., Hordiichuk, L. M., Kamratska, O. I., & Denkovich, B. S. (2020). Comfort and cow behavior during periods of intense precipitation. Ukrainian Journal of Ecology, 10(6), 98–102.
Brezvyn, O. M., Guta, Z. A., Gutyj, B. V., Fijalovych, L. M., Karpovskyi, V. I., Shnaider, V. L., Farionik, T. V., Dankovych, R. S., Lisovska, T. O., Bushuieva, I. V., Parchenko, V. V., Magrelo, N. V., Slobodjuk, N. M., Demus, N. V., & Leskiv, K. Y. (2021). The influence of HamekoTox on the morphological and biochemical indices of the blood of laying hens in spontaneous fumonisin toxicosis. Ukrainian Journal of Ecology, 11(2), 249–253.
Castellini, C., Ruggeri, S., Mattioli, S., Bernardini, G., Macchioni, L., Moretti, E., & Collodel, G. (2014). Long-term effects of silver nanoparticles on reproductive activity of rabbit buck. Systems Biology in Reproductive Medicine, 60(3), 143–150.
de Blas, J. C., & Gonzalez-Mateos, G. (2010). Feed formulation. In: de Blas, C., & Wiseman, J. (Eds.). Nutrition of the rabbit. Second ed. CABI International. Wallingford. Pp. 222–232.
Delgado, R., Nicodemus, N., Abad-Guamán, R., Menoyo, D., García, R., & Carabaño, R. (2018). Effect of arginine and glutamine supplementation on performance, health and nitrogen and energy balance in growing rabbits. Animal Feed Science and Technology, 247, 63–73.
Elahi, U., Wang, J., Ma, Y., Wu, S., Qi, G., & Zhang, H. (2020). The response of broiler chickens to dietary soybean meal reduction with glycine and cysteine inclusion at marginal sulfur amino acids (SAA) deficiency. Animals, 10(9), 1686.
Ema, M., Okuda, H., Gamo, M., & Honda, K. (2017). A review of reproductive and developmental toxicity of silver nanoparticles in laboratory animals. Reproductive Toxicology, 67, 149–164.
Fathi, M., Abdelsalam, M., Al-Homidan, I., Ebeid, T., El-Zarei, M., & Abou-Emera, O. (2017). Efect of probiotic supplementation and genotype on growth performance, carcass traits, hematological parameters and immunity of growing rabbits under hot environmental conditions. Animal Science Journal, 88(10), 1644–1650.
Gary, T., Pichler, M., Belaj, K., Hafner, F., Gerger, A., Froehlich, H., Eller P., Rief P., Hackl, G., & Pilger, E. (2013). Platelet-to-lymphocyte ratio: A novel marker for critical limb ischemia in peripheral arterial occlusive disease patients. PLoS One, 8(7), 676–688.
Gidenne, T., Garreau, H., Drouilhet, L., Aubert, C., & Maertens, L. (2017). Improving feed efficiency in rabbit production, a review on nutritional, technico-economical, genetic and environmental aspects. Animal Feed Science and Technology, 225, 109–122.
Harkness, J. E., Turner, P. V., Vande Woude, S., & Wheler, C. L. (2013). Haematology, clinical chemistry, and urinalysis. In: Biology and medicine of rabbits and rodents. 5th ed. Wiley, Ames. Pp. 116–131.
Khalak, V., Gutyj, B., Stadnytska, O., Shuvar, I., Balkovskyi, V., Korpita, H., Shuvar, A., & Bordun, О. (2021). Breeding value and productivity of sows of the Large White breed. Ukrainian Journal of Ecology, 11(1), 319–324.
Lesyk, Y., Ivanytska, A., Kovalchuk, I., Monastyrska, S., Hoivanovych, N., Gutyj, B., Zhelavskyi, M., Hulai, O., Midyk, S., Yakubchak, O., & Poltavchenko, T. (2020). Hematological parameters and content of lipids in tissues of the organism of rabbits according to the silicon connection. Ukrainian Journal of Ecology, 10(1), 30–36.
Marín-García, P. J., Ródenas, L., Martínez-Paredes, E. M., Cambra-López, M., Blas, E., & Pascual, J. J. (2020c). A moderate protein diet does not cover the requirements of growing rabbits with high growth rate. Animal Feed Science and Technology, 264, 114495.
Marín-García, P. J., Ródenas, L., Martínez-Paredes, E., Blas, E., & Pascual, J. J. (2020b). Plasmatic urea nitrogen in growing rabbits with different combinations of dietary levels of lysine, sulphur amino acids and threonine. Animals, 10(6), 946.
Martyshuk, T. V., Gutyj, B. V., Zhelavskyi, M. M., Midyk. S. V., Fedorchenko, A. M., Todoriuk, V. B., Nahirniak, T. B., Kisera, Y. V., Sus, H. V., Chemerys, V. A., Levkivska, N. D., & Iglitskej, I. I. (2020). Effect of Butaselmevit-Plus on the immune system of piglets during and after weaning. Ukrainian Journal of Ecology, 10(2), 347–352.
Meshreky, S. Z., Allam, S. M., ElManilawi, M., & Amin, H. (2015). Effect of dietary supplemental zinc source and level on growth performance, digestibility coefficients and immune response of New Zealand white rabbits. Egyptian Journal of Nutrition and Feeds, 18(2), 383–390.
Mínguez, C., Sánchez, J. P., Nagar, A. G., Ragab, M., & Baselga, M. (2016). Growth traits of four maternal lines of rabbits founded on different criteria: Comparisons at foundation and at last periods after selection. Journal of Animal Breeding and Genetics, 133(4), 303–315.
Mylostyvyi, R., Sejian, V., Izhboldina, O., Kalinichenko, O., Karlova, L., Lesnovskay, O., Begma, N., Marenkov, O., Lykhach, V., Midyk, S., Cherniy, N., Gutyj, B., & Hoffmann, G. (2021). Changes in the spectrum of free fatty acids in blood serum of dairy cows during a prolonged summer heat wave. Animals, 11(12), 3391.
Ognik, K., Cholewińska, E., Czech, A., Kozłowski, K., Wlazło, L., Nowakowicz-Dębek, B., Szlązak, R., & Tutaj, K. (2016). Effect of silver nanoparticles on the immune, redox, and lipid status of chicken blood. Czech Journal of Animal Science, 61, 450–461.
Oloruntola, O. D., Ayodele, S. O., Jimoh, O. A., & Agbede, J. O. (2019). Dietary cassava peel meal, methionine, and multi-enzyme supplementation in rabbits’ nutrition: Effect on growth, digestibility, and carcass traits. The Journal of Basic and Applied Zoology, 80, 46.
Raheem, H. Q. (2019). Study efect of silver nanoparticles on some blood parameters in rabbits. Biochemical and Cellular Archives, 18, 267–269.
Reda, F. M., El-Saadony, M. T., Elnesr, S. S., Alagawany, M., & Tufarelli, V. (2020). Effect of dietary supplementation of biological curcumin nanoparticles on growth and carcass traits, antioxidant status, immunity and caecal microbiota of Japanese quails. Animals, 10(5), 754.
Saleh, A. A., & El-Magd, M. A. (2018). Beneficial effects of dietary silver nanoparticles and silver nitrate on broiler nutrition. Environmental Science and Pollution Research, 25(27), 27031–27038.
Samak, D. H., El-Sayed, Y. S., Shaheen, H. M., El-Far, A. H., Abd El-Hack, M. E., Noreldin, A. E., El-Naggar, K., Abdelnour, S. A., Saied, E. M., El-Seedi, H. R., Aleya, L., & Abdel-Daim, M. M. (2020). Development toxicity of carbon nanoparticles during embryogenesis in chicken. Environmental Science and Pollution Research, 27(16), 19058–19072.
Sawyer, J. E., Lang, B. J., & Barker, D. W. (2011). Sulfur fertilization response in Iowa corn production. Better Crops, 95(2), 8–11.
Swelum, A. A., Elbestawy, A. R., El-Saadony, M. T., Hussein, E. O. S., Alhotan, R., Suliman, G. M., Taha, A. E., Ba-Awadh, H., El-Tarabily, K. A., & Abd El-Hack, M. E. (2021). Ways to minimize bacterial infections, with special reference to Escherichia coli, to cope with the first-week mortality in chicks: An updated overview. Poultry Science, 100(5), 101–139.
Syrvatka, V., Rozgoni, I., Slyvchuk, Y., Milovanova, G., Hevkan, I., & Matyukha, I. (2014). Efects of silver nanoparticles in solution and liposomal form on some blood parameters in female rabbits during fertilization and early embryonic development. Journal of Microbiology, Biotechnology and Food Sciences, 3, 274–278.
Trocino, A., García, J., Carabaño, R., & Xiccato, G. (2013). A meta-analysis on the role of soluble fibre in diets for growing rabbits. World Rabbit Science, 21(1), 1–15.
Vadalasetty, K. P., Lauridsen, C., Engberg, R. M., Vadalasetty, R., Kutwin, M., Chwalibog, A., & Sawosz, E. (2018). Influence of silver nanoparticles on growth and health of broiler chickens after infection with Campylobacter jejuni. BMC Veterinary Research, 14, 1.
Van Milgen, J., & Dourmad, J. Y. (2015). Concept and application of ideal protein for pigs. Journal of Animal Science and Biotechnology, 6(1), 15.
Vasylyev, D., Priimenko, B., Aleksandrova, K., Mykhalchenko, Y., Gutyj, B., Mazur, I., Magrelo, N., Sus, H., Dashkovskyy, O., Vus, U., & Kamratska, O. (2021). Investigation of the acute toxicity of new xanthine xenobiotics with noticeable antioxidant activity. Ukrainian Journal of Ecology, 11(1), 315–318.
Villamide, M. J., García, A. I., Llorente, A., & Carabaño, R. (2013). Ileal vs. faecal amino acid digestibility in concentrates and fibrous sources for rabbit feed formulation. Animal Feed Science and Technology, 182, 100–110.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons «Attribution» 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.