Specifics of the morphological identification of the pathogen of passaluariasis of rabbits

  • A. Khorolskyi Poltava State Agrarian University
  • V. Yevstafieva Poltava State Agrarian University
  • S. Kravchenko Poltava State Agrarian University
  • M. Pishchalenko Poltava State Agrarian University
  • Y. Vakulenko Poltava State Agrarian University
  • B. Gutyj Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj
Keywords: Passalurus ambiguus; parasitic nematodes; diagnostics; differential characters.


Passalurus ambiguus (Nematoda, Oxyuroidae) is one of the most common parasites of digestive tract of domestic and wild rabbits and rodents. Passalurus spp. are cosmopolitan, and the mature nematodes are localized in the appendages and large intestine of animals. The passaluariasis infections remain quite relevant in modern rabbit breeding, because the disease is highly contagious, with the possibility of unlimited spread inducing significant economic losses. The aim of the study was to determine the identifying morphological species characteristics of P. ambiguus nematodes, which were isolated from the colon of domestic rabbits (Oryctolagus cuniculus domesticus). The identifying characters of Passalurus include morphological features, namely the presence of a spherical bulb in the esophagus, and the presence of three teeth in the oral capsule. Males of P. ambiguus have a characteristic structure of the tail end, namely the presence of papillary protrusions and a massive narrowed part with a spicule handle, and five papillae around the cloaca. Twenty-eight metric indicators are proposed that characterize the size of the body, oral capsule, esophagus in different parts of it, the tail end, spicules, the location of the cloaca. Two different morphotypes of mature females of P. ambiguus were revealed – short-tailed and long-tailed, which were larger by 19 indicators (by 3.2–43.6%). The morphotypes of P. ambiguus females are characterized; the length of the tail, body length and width in different areas, depth of the oral capsule, esophageal size and its structural elements, the location of the vulva and nerve ring, the width of the caudal process. At the same time, the parameters of esophageal bulb length and caudal process in short-tailed females were higher (by 13.9% and 11.9%, respectively) than in the long-tailed females. A total of 25 morphometric parameters were proposed for the identification of P. ambiguus females. The obtained data indicate the feasibility of further research on the causes of occurrence of such morphotypes in P. ambiguus females and the need to take into account the existence of such forms of females for their differential diagnosis taking into account their proposed morphometric parameters.


Abdel-Gaber, R., Ataya, F., Fouad, D., Daoud, M., & Alzuhairy, S. (2019). Prevalence, morphological and molecular phylogenetic analyses of the rabbit pinworm, Passalurus ambiguus Rudolphi 1819, in the domestic rabbits Oryctolagus cuniculus. Acta Parasitologica, 64(2), 316–330.

Ashmawy, K. I., El-Sokkary, M. Y., Abu-Akkada, S. S., & Dewair, A. W. (2010). Incidence of Passalurus ambiguus in domestic rabbits in Behera Province. Austral Journal of Veterinary Sciences, 30, 115–120.

Bin, Z., & Chunsheng, B. (1987). Scanning electron microscopic observations of the integumental surface of adult Passalurus ambiguus. Acta Zoologica Sinica, 33(4), 383–384.

Boag, B., & Iason, G. (1986). The occurrence and abundance of helminth parasites of the mountain hare Lepus timidus (L.) and the wild rabbit Oryctolagus cuniculus (L.) in Aberdeenshire, Scotland. Journal of Helminthology, 60(2), 92–98.

Boyko, O. O., Gugosyan, Y. A., Shendrik, L. I., & Brygadyrenko, V. V. (2019). Intraspecific morphological variation in free-living stages of Strongyloides papillosus (Nematoda, Strongyloididae) parasitizing various mammal species. Vestnik Zoologii, 53(4), 313–324.

Cullere, M., & Dalle Zotte, A. (2018). Rabbit meat production and consumption: State of knowledge and future perspectives. Meat Sience, 143, 137–146.

Dalle Zotte, A. (2014). Rabbit farming for meat purposes. Animal Frontiers, 4, 62–67.

Danheim, B. L., & Ackert, J. E. (1929). On the anatomy of the nematode Passalurus ambiguus (Rudolphi). Transactions of the American Microscopical Society, 48, 80–85.

Ebino, K. Y., Shutoh, Y., & Takahashi, K. W. (1993). Coprophagy in rabbits: Autoingestion of hard feces. Jikken Dobutsu. Experimental Animals, 42(4), 611–613.

Eira, C., Torres, J., Miquel, J., & Vingada, J. (2007). The helminth parasites of the wild rabbit Oryctolagus cuniculus and their effect on host condition in Dunas de Mira, Portugal. Journal of Helminthology, 81(3), 239–246.

Eslami, A., Changizy, E., & Moghadam, M. (2000). Prevalence of helminth infections in the cape hare (Lepus capensis) in Iran. Veterinary Research Communications, 24(7), 455–458.

Fayek, S. A., El Bahy, N. M., & El Khair, A. (1995). Contributions on the Passalurus ambiguus life cycle and scanning electron microscopy studies. Veterinary Medical Journal, 43(4), 449–453.

Foronda, P., Valladares, B., Lorenzo-Morales, J., Ribas, A., Feliu, C., & Casanova, J. C. (2003). Helminths of the wild rabbit (Oryctolagus cuniculus) in Macaronesia. Journal of Parasitology, 89(5), 952–957.

Frank, R., Kuhn, T., Mehlhorn, H., Rueckert, S., Pham, D., & Klimpel, S. (2013). Parasites of wild rabbits (Oryctolagus cuniculus) from an urban area in Germany, in relation to worldwide results. Parasitology Research, 112(12), 4255–4266.

Georgieva, K., Yoneva, A., Mizinska-Boevska, Y., & Todev, I. (2005). Ultrastructure of the contact surfaces of Passalurus ambiguus (Rudolphi, 1819) (Nematoda). Acta Biologica Hungarica, 56, 297–303.

González-Acuña, D., Rebolledo, P., Skewes, O., Moreno, L., & Castro, D. (2005). Parásitos de la liebre (Lepus europaeus Pallas, 1778): Estudio en dos zonas geográficas de Chile. Parasitologia Latinoamericana, 60, 174–177.

Gugosyan, Y. A., Boyko, O. O., & Brygadyrenko, V. V. (2019). Morphological variation of four species of Strongyloides (Nematoda, Rhabditida) parasitising various mammal species. Biosystems Diversity, 27(1), 85–98.

Gvozdev, E. V., Kontrimavichus, V. L., Ryzhikov, K. M., & Shaldybin, L. S. (1970). Oprelitel’ gel’mintov zajceobraznyh SSSR [Key to the helminth parasites of Lagomorpha in the USSR]. Nauka, Moscow (in Russian).

Hajipour, N., & Zavarshani, M. (2020). Ectoparasites and endoparasites of New Zealand white rabbits from North West of Iran. Iranian Journal of Parasitology, 15(2), 266–271.

Hall, M. C. (1916). Nematode parasites of mammals of the orders Rodentia, Lagomorpha, and Hyracoidea. Proceedings of the United States National Museum, 50(2131), 1–258.

Hobbs, R. P., Twigg, L. E., Alliot, A. D., & Wheeler, A. G. (1999). Evaluation of the associatism of parasitism with mortality of wild rabbits Oryctolagus cuniculus (L.) in South Western Australia. Journal of Parasitology, 85(5), 803–808.

Hugot, J.-P., Bain, O., & Cassone, J. (1983). Sur le genre Passalurus (Oxyuridae: Nematoda) parasite de Leporidés. Systematic Parasitology, 5(4), 305–316.

Ilić, T., Stepanović, P., Nenadović, K., & Dimitrijević, S. (2018). Improving agricultural production of domestic rabbits in Serbia by follow-up study of their parasitic infections. Iranian Journal of Veterinary Research, 19(4), 290–297.

Ivashkin, V. M., Kontrimavichus, V. L., & Nazarova, N. S. (1971). Metody sbora i izuchenija gel’mintov nazemnyh pozvonochnyh [Method for collection and study of helminths of terrestrial vertebrates]. Nauka, Moscow (in Russian).

Kornaś, S., Kowal, J., Wierzbowska, I., Basiaga, M., Nosal, P., & Niedbała, P. (2015). The alice – “Follow the white rabbit” – parasites of farm rabbits based on coproscopy. Annals of Parasitology, 61(4), 257–261.

Li, S., Cui, P., Fang, S. F., Lin, R. Q., Zou, F. C., & Zhu, X. Q. (2014). Sequence variability in four mitochondrial genes among rabbit pinworm (Passalurus ambiguus) isolates from different localities in China. Mitochondrial DNA, 26, 501–504.

Li, S., Zeng, W., Li, R., Hoffman, L. C., He, Z., Sun, Q., & Li, H. (2018). Rabbit meat production and processing in China. Meat Sience, 145, 320–328.

Madsen, M. (1986). A review of various parasites of rabbits. Nordisk Veterinaermedicin, 38(6), 333–351.

Motamedi, G., Moharami, M., Paykari, H., Eslampanah, M., & Omraninava, A. (2014). A survey on the gastrointestinal parasites of rabbit and guinea pig in a laboratory animal house. Archives of Razi Institute, 69(1), 77–81.

Mykhailiutenko, S. M., Kruchynenko, O. V., Klymenko, O. S., Serdioucov, J. K., Dmytrenko, N. I., & Tkachenko, V. V. (2019). Pathomorphological changes in the large intestine of rabbits parasitised by Passalurus ambiguus (Nematoda, Oxyuridae). Regulatory Mechanisms in Biosystems, 10(1), 69–74.

Nosal, P., Kowal, J., Nowosad, B., Bieniek, J., & Kowalska, D. (2009). Dynamics of endoparasite infections in rabbits at different rearing regimes. Wiadomosci Parazytologiczne, 55(2), 173–177.

Pinto, R. M., Gomez, D. C., Menezes, R. C., Gomez, C. T., & Noronha, D. (2004). Helminths of rabbits (Lagomorpha, Leporidae) deposited in the helminthological collection of the Oswaldo Cruz Institute. Revista Brasileira de Zoologia, 21(3), 599–604.

Rinaldi, L., Russo, T., Schioppi, M., Pennacchio, S., & Cringoli, G. (2007). Passalurus ambiguus: New insights into copromicroscopic diagnosis and circadian rhythm of egg excretion. Parasitology Research, 101, 557–561.

Sheng, L., Cui, P., Fang, S., Lin, R., Zou, F., & Zhu, X. (2014). Sequence variability in four mitochondrial genes among rabbit pinworm (Passalurus ambiguus) isolates from different localities in China. Mitochondrial DNA, 26(4), 501–504.

Skjabin, K. I., Shikhobalova, N. P., & Lagodovskaya, E. A. (1967). Osnovy nematodologii. Oksiuraty [Essentials of nematodology. Oxyurates]. Nauka, Moscow (in Russian).

Skrjabin, K. I. (1928). Metod polnyh gel’mintologicheskih vskrytij pozvonochnyh, vkljuchaja cheloveka [The method of complete helminthological autopsy of vertebrates, including humans]. Moscow State University, Moscow (in Russian).

Sultan, K., Elhawary, N. M., Sourour, S. S. G., & Sharaf, H. (2015). Observations of the rabbit pinworm Passalurus ambiguus (Rudolphi, 1819) in domestic rabbits (Oryctolagus cuniculus) in Egypt using a scanning electron microscope. Tropical Biomedicine, 32(4), 745–752.

Taffs, L. F. (1976). Pinworm infections in laboratory rodents: A review. Laboratory Animals, 10(1), 1–13.

Vicente, J. J., Rodrigues, H. O., Gomes, D. C., & Pinto, R. M. (1997). Nematóides do Brasil. Parte V: Nematóides de mamíferos. Revista Brasileira de Zoologia, 14(1), 117–120.

Yagoob, G., & Hossein, H. (2011). Prevalence rate of endoparasites in wild rabbits of East-Azerbaijan Province, Iran. Annals of Biological Research, 2, 31–35.

Yevstafieva, V. O., Prykhodko, Y. О., Kruchynenko, O. V., Mykhailiutenko, S. M., & Kone, M. S. (2020). Biological specifics of exogenous development of Oxyuris equi nematodes (Nematoda, Oxyuridae). Biosystems Diversity, 28(2), 125–130.

How to Cite
Khorolskyi, A., Yevstafieva, V., Kravchenko, S., Pishchalenko, M., Vakulenko, Y., & Gutyj, B. (2021). Specifics of the morphological identification of the pathogen of passaluariasis of rabbits . Regulatory Mechanisms in Biosystems, 12(4), 702-709. https://doi.org/10.15421/022197