Regional anesthesiological support for the implementation of surgical interventions in the abdomen of dogs

  • D. V. Sliusarenko Kharkiv State Zooveterinary Academy
  • М. G. Ilnitskiy Bila Tserkva National Agrarian University
  • D. D. Bilyi Dnipro State Agrarian and Economic University
  • O. M. Bobrytska Kharkiv State Zooveterinary Academy
  • L. O. Kovalova Polissia National University
Keywords: epidural blockade; local anaesthetics; bupivacaine; wound healing; stress markers; cytokines.


Widespread use of surgical interventions in the abdomen of dogs determines the relevance of the study. The research tested the influence of therapeutic epidural blockade with 0.2% bupivacaine after surgical interventions on the background of potentiated local anaesthesia on the body of clinically healthy dogs undergoing ovariohysterectomy. In the control group postoperative analgesia was performed with the nonsteroidal anti-inflammatory drug rimadyl on the background of potentiated thiopental anaesthesia. Wound healing rates were determined in 10 animals of both control and experimental groups; blood counts and the level of inflammatory response were indicated in 5 animals of each group. The dynamics of reparative processes and complete wound healing in dogs of the experimental group occurred earlier than in animals of the control group, which confirmed the fact that the use of bupivacaine had a beneficial effect on tissue regeneration. The results of morphological and biochemical parameters of blood in animals of the experimental group manifested only an increase of leukocytes by 1.58 times, fibrinogen by 2.02 times, while in dogs of the control group the level of erythrocytes, haemoglobin and hematocrit decreased, leukocytes increased by 1.74 times, platelets decreased by 2.87 times and fibrinogen increased by 1.72 times. Among the stress response markers in the control group there was a 1.94 times increase in glucose level after surgery. In the experimental group there was a decrease in the intensity of the inflammatory reaction in dogs, accompanied by an increase of anti-inflammatory interleukin 4 (IL-4) only on the seventh day – 1.86 times, while in the control group the level of anti-inflammatory interleukin 1 receptor antagonist (IL-1RA) after three days increased by 2.30 times, after seven days – by 2.48 times, and the level of proinflammatory interleukin 6 (IL-6) after surgery increased by 2.57 times. It will be promising to conduct further research on the influence of therapeutic blockades with bupivacaine in animals in cases of various surgical interventions and pathological conditions, which will lead to faster recovery of animals and alleviate pathological processes.


Aggarwal, N. (2018). Local anesthetics systemic toxicity association with exparel (bupivacaine liposome) ‒ a pharmacovigilance evaluation. Expert Opinion in Drug Safety, 17(6), 581–587.

Bahrenberg, A. І., Dzikiti, B. T., Fosgate, G. T., Stegmann, F. G., Tacke, S. P., & Rioja, E. (2015). Antinociceptive effects of epidural magnesium sulphate alone and in combination with morphine in dogs. Veterinary Anaesthesia and Analgesia, 42(3), 319‒328.

Bomberg, H., Kubulus, C., Herberger, S., Wagenpfeil, S., Kessler, P., Steinfeldt, T., Standl, T., Gottschalk, A., Stork, J., Meissner, W., Birnbaum, J., Koch, T., Sessler, D. I., Volk, T., & Raddatz, A. (2016). Tunnelling of thoracic epidural catheters is associated with fewer catheter-related infections: A retrospective registry analysis. British Journal of Anaesthesia, 116(4), 546‒553.

Burbridge, M., & Jaffe, R. A. (2015). Exparel®: A new local anesthetic with special safety concerns. Anesthesia and Analgesia, 121(4), 1113–1114.

Butterworth, J. F. (2010). Models and mechanisms of local anesthetic cardiac toxicity: A review. Regional Anesthesia and Pain Medicine, 35(2), 167–176.

Campoy, L., & Read, M. R. (2013). Small animal regional anaesthesia and analgesia. Wiley-Blackwell, Ames, Chichester, Oxford.

Carregaro, A. B., Freitas, G. C., Lopes, C., Lukarsewskic, R., Tamiozzoc, F. S., & Santosc, R. R. (2016). Evaluation of analgesic and physiologic effects of epidural morphine administered at a thoracic or lumbar level in dogs undergoing thoracotomy. Veterinary Anaesthesia and Analgesia, 41(2), 205–211.

Chahar, Р., & Cummings, K. C. (2012). Liposomal bupivacaine: A review of a new bupivacaine formulation. Journal of Pain Research, 5, 257–264.

Discepola, P., Bouhara, M., Kwon, M., Siddiqui, B. A., Whitwell, T. A., Sanghvi, S. Y., Cook, K. D., Moore, R. E., Korban, A., & Eloy, J. D. (2020). Exparel® (Long-acting liposomal bupivacaine) use for popliteal nerve block in postoperative pain control after ankle fracture fixation. Pain Research and Management, 2020, 5982567.

Docenko, V. V. (2011). Vlijanie razlichnykh metodik anestezii na vyrazhennost’ vospalitel’nogo otveta pri operacijah v plasticheskoj hirurgii [Influence of various anesthesia techniques on the severity of the inflammatory response during plastic surgery operations]. Ukrajins’kyj Zhurnal Ekstremal’noji Medycyny imtys G. O. Mozhaеva, 12(3), 55–59 (in Russian).

Epstein, M., Rodan, I., Griffenhagen, G., Kadrlik, J., Petty, M., Robertson, S., & Simpson, W. (2015). AAHA/AAFP pain management guidelines for dogs and cats. Journal of the American Animal Hospital Association, 51(2), 67–84.

Fesenko, V. S. (2002) Blokady nerviv [Nerve blockades]. Ekskliuzyv, Kharkiv (in Ukrainian).

Fesenko, V. S. (2010). Topografo-anatomichne ta klinichne obgruntuvannia pidvyshhennja efektyvnosti ta bezpechnosti regionarnogo zneboljuvannja [Topographic-anatomical and clinical substantiation of increase of efficiency and safety of regional anesthesia]. Navchalno-Naukovyi Vydavnycho-Polihrafichnyi Tsentr KhNURE, Dnipropetrovsk (in Ukrainian).

Fesenko, V. S., Donchak, J. D., Perepelica, E. E., & Rudachenko, O. J. (2007). Intoksikacija bupivakainom: Opisanie sluchaja i literaturnyj obzor [Bupivacaine intoxication: Case description and literature review]. Medicina Neotlozhnyh Sostojanij, 6(13), 90–94 (in Russian).

Filipenko, V. A., Leont’jeva, F. S., Morozenko, D. V., & Korzh, I. V. (2012). Vmist cytokiniv u syrovatci krovi hvoryh na osteoartroz [The content of cytokines in the serum of patients with osteoarthritis]. Ortopedyja, Travmatologyja y Protezyrovanye, 3, 52–55 (in Ukrainian).

Ghadirian, S., Vesal, N., Maghsoudi, B., & Akhlagh, S. H. (2016). Comparison of lidocaine, lidocaine-morphine, lidocaine-tramadol or bupivacaine for neural blockade of the brachial plexus in fat-tailed lambs. Veterinary Anaesthesia аnd Analgesia, 43(1), 109–116.

Grubb, T., & Lobprise, H. (2020). Local and regional anaesthesia in dogs and cats: Overview of concepts and drugs (Part 1). Veterinary Medicine and Science, 6, 209–217.

Gutierrez-Blanco, E., Victoria-Mora, J. M., Ibancovichi-Camarillo, J. A., Sauri‐Arceo, C. H., Bolio‐González, M. E., Acevedo‐Arcique, C. M., Cano, G. M., & Steagall, P. V. M. (2015). Postoperative analgesic effects of either a constant rate infusion of fentanyl, lidocaine, ketamine, dexmedetomidine, or the combination lidocaine-ketamine-dexmedetomidine after ovariohysterectomy in dogs. Veterinary Anaesthesia and Analgesia, 42(3), 309–318.

Hansen, B., Lascelles, B. D., Thomson, A., & De Puy, V. (2013). Variability of performance of wound infusion catheters. Veterinary Anaesthesia and Analgesia, 40(3), 308–315.

Hu, D. D., Onel, Е., & Singla, N. (2013). Pharmacokinetic profile of liposome bupivacaine injection following a single administration at the surgical site. Clinical Drug Investigation, 33, 109–115.

Johns, J., Harrison, T. K., Steffel, L., Howard, S. K., Kim, T. E., Kou, A., & Mariano, E. R. (2014). A pilot in vitro evaluation of the “air test” for perineural catheter tip localization by a novice regional anesthesiologist. Journal Ultrasound Medicine, 33, 2197–2200.

Kalchofner Guerrero, K. S., Reichler, I. M., Schwarz, А., Jud, R. S., Hässig, M., & Bettschart‐Wolfensberger, R. (2014). Alfaxalone or ketamine-medetomidine in cats undergoing ovariohysterectomy: A comparison of intra-operative parameters and post-operative pain. Veterinary Anaesthesia and Analgesia, 41(6), 644–653.

Kerroll, G. L. (2009). Anesteziologija i anal’gezija melkih domashnih zhivotnyh [Small animal anesthesiology and analgesia]. Akvarium-Print, Moscow (in Russian).

Kibkalo, D. V. (2017). Analiz cytokinovogo statusu syrovatky krovi koriv za subklinichnoi' formy ketozu [Analysis of cytokins status in blood serum of cows with subclinical form of of ketosis]. Biologija Tvaryn, 19(3), 51–54 (in Ukrainian).

Koch, T., Sessler, D. I., & Volk, T. (2016). Tunnelling of thoracic epidural catheters is associated with fewer catheter-related infections: A retrospective registry analysis. British Journal of Anaesthesia, 116(4), 546‒553.

Lamont, L. A., & Lemke, K. A. (2008). The effects of medetomidine on radial nerve blockade with mepivacaine in dogs. Veterinary Anaesthesia and Analgesia, 35(1), 62–68.

Markova, L., Umek, N., Horvat, S., Hadžić, A., Kuroda, M., Pintarič, T. S., Mrak, V., & Cvetko, E. (2020). Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice. BMC Veterinary Research, 16(1), 247.

Marzok, M. A., & Elkhodery, S. A. (2015). Comparative analgesic and sedative effects of tramadol, tramadol-lidocaine and lidocaine for caudal epidural analgesia in donkeys (Equus asinus). Veterinary Anaesthesia and Analgesia, 42(2), 215–219.

Mastrocinque, S. І., & Fantoni, D. T. (2003). A comparison of preoperative tramadol and morphine for the control of early postoperative pain in canine ovariohysterectomy. Veterinary Anaesthesia and Analgesia, 30(4), 220–228.

Morgan Jr., G. E., & Maged, S. M. (2000). Klinicheskaja anesteziologija [Clinical Anesthesiology]. Binom Nevskij Dialekt, Moscow, Sankt-Peterburg. Vol. 1 (in Russian).

Orlov, G. S. (2011). Lechebnye medikamentoznye blokady v travmatologii i ortopedii [Therapeutic drug blockade in traumatology and orthopedics]. Medicina Neotlozhnyh Sostojanij, 32–33, 30–33 (in Russian).

Otero, P. E., Verdier, N., Ceballos, M. R., Tarragona, L., Flores, M., & Portela, D. A. (2014). The use of electrical stimulation to guide epidural and intrathecal needle advancement at the L5–L6 intervertebral space in dogs. Veterinary Anaesthesia and Analgesia, 41(5), 543–547.

Otero, P. E., Zaccagnini, A. S., Fuensalida, S. E., Verdier, N., Sclocco, M., & Portela, D. A. (2014). Use of electrical nerve stimulation to monitor lumbosacral epidural needle placement in cats. Veterinary Anaesthesia Analgesia, 41(3), 325–329.

Panko, I. S., Vlasenko, V. M., & Levchenko, V. I. (1994). Patogenetychna terapija pry zapal’nyh procesah u tvaryn [Pathogenetic therapy in inflammatory processes in animals]. Kyiv (in Ukrainian).

Rioja, G. E. (2015). Local anesthetics. In: Grimm, K. A., Lamont, L. A., Tranquilli, W. J., Greene, S. A., & Robertson, S. A. (Eds.). Veterinary anesthesia and analgesia: The fifth edition of lumb and jones. John Wiley & Sons, Ames. Pp. 332–354.

Romano, М., Portela, D. A., Breghi, G., & Otero, Р. Е. (2016). Stress-related biomarkers in dogs administered regional anaesthesia or fentanyl for analgesia during stifle surgery. Veterinary Anaesthesia and Analgesia, 43(1), 44–54.

Rublenko, M. V. Andrijec, V. G., & Berezovs’kyj, A. V. (2014). Kompleksnyj metod likuvannja nekrobakterioznyh urazhen’ kincivok u velykoji rogatoji hudoby [A comprehensive method of treatment of necrobacterial lesions of the extremities in cattle]. Naukovyj Visnyk Veterynarnoji Medycyny, 108, 194–197 (in Ukrainian).

Rublenko, M. V., & Vlasenko, S. A. (2001). Kompleksnyj metod likuvannja gnijno-nekrotychnyh urazhen’ diljanky pal’cia u koriv [A comprehensive method of treatment of purulent-necrotic lesions of the finger in cows]. Naukovyj Visnyk Nacional’nogo Agrarnogo Universytetu, 38, 54–57 (in Ukrainian).

Sarotti, D., Rabozzi, R., & Franci, P. (2015). Comparison of epidural versus intrathecal anaesthesia in dogs undergoing pelvic limb orthopaedic surgery. Veterinary Anaesthesia and Analgesia, 42(4), 405–413.

Senera, A., Erkinb, Y., & Senerc, A. (2015). In vitro comparison of epidural bacteria filters permeability and screening scanning electron microscopy. Brazilian Journal of Anesthesiology, 65(6), 491–496.

Shhekyna, K. G., Shtrygol’, S. J., & Drogovoz, S. M. (2013). Dosiagnennia ta perspektyvy cytokinovoji ta antycytokinovoji terapiji [Achievements and prospects of cytokine and anticytokine therapy]. Naukovyj Zhurnal MOZ Ukrajiny, 1(2), 121–129 (in Ukrainian).

Sljusarenko, D. V., Il’nic’kyj, M. G., & Segodin, O. B. (2016). Porivnjal’na harakterystyka zbudlyvosti tkanyn u sobak za epidural’noji anesteziji 0.2% bupivakajinom, sedaciji ksylazynom ta tiopenatovogo narkozu [Comparative characteristics of tissue excitability in dogs under epidural anesthesia with 0.2% bupivacaine, sedation with xylazine and thiopenate anesthesia]. Naukovo-Tehnichnyj Biuleten’ Naukovo-Doslidnogo Centru Biobezpeky ta Ekologichnogo Kontroliu Resursiv APK Dnipropetrovs’kogo Derzhavnogo Agrarno-Ekonomichnogo Universytetu, 4(3), 25–28 (in Ukrainian).

Snyder, L. B., & Snyder, C. J. (2016). Effects of buprenorphine added to bupivacaine infraorbital nerve blocks on isoflurane minimum alveolar concentration using a model for acute dental/oral surgical pain in dogs. Journal of Veterinary Dentistry, 33(2), 90–96.

Son, W. G., Jang, M., Jo, S. M., Yoon, J., & Leea, I. (2015). The volume effect of lidocaine on thoracic epidural anesthesia in conscious Beagle dogs. Veterinary Anaesthesia and Analgesia, 42(4), 414–424.

Son, W. G., Jang, M., Yoon, J., Lee, L. Y., & Leea, I. (2014). The effect of epidural injection speed on epidural pressure an distribution of solution in anesthetized dogs. Veterinary Anaesthesia and Analgesia, 41(5), 526–533.

Subbotin, V. M., Subbotina, S. G., & Aleksandrov, I. D. (2000). Sovremennye lekarstvennye sredstva v veterinarii [Modern medicines in veterinary medicine]. Feniks, Rostov-na-Donu (in Russian).

Suslov, V. V., Hizhnjak, A. A., & Tarabrin, O. A. (2011). Epidural’naja anestezija i analgezija [Epidural anesthesia and analgesia]. Sim, Kharkov (in Russian).

Trabelsi, B., Charfi, R., Bennasr, L., Ben Marzouk, S., Eljebari, H., Jebabli, N., Ben Sassi, M., Trabelsi, S., & Maghrebi, H. (2017). Pharmacokinetics of bupivacaine after bilateral ultrasound-guided transversus abdominis plane block following cesarean delivery under spinal anesthesia. International Journal of Obstetric Anesthesia, 32, 17–20.

Viscasillas, J., Sanchis, S., & Sneddon, С. (2014). Ultrasound guided epidural catheter placement in a dog. Veterinary Anaesthesia And Аnalgesia, 41(3), 330–331.

Vlasenko, V. M., & Tyhonjuk, L. A. (2000). Veterynarna anesteziologija [Veterinary anesthesiology]. Bilotserkivskyi Derzhavnyi Ahrarnyi Universytet, Bila Cerkva (in Ukrainian).

Wagatsuma, J. T., Deschk, M., Floriano, B. P., Ferreira, J. Z., Fioravanti, H., Gasparello, I. F., & Oliva, V. N. L. S. (2014). Comparison of anesthetic efficacy and adverse effects associated with peribulbar injection of ropivacaine performed with and without ultrasound guidance in dogs. American Journal of Veterinary Research, 75(12), 1040–1048.

Wakoff, T. I., Mencalha, R., Souza, N. S., Santos Sousa, C. A., Inácio Sousa, M. D., & Scherer, P. O. (2014). Bupivacaine 0.25% versus ropivacaine 0.25% in brachial plexus block in dogs of beagle breed. Schearer Ciências Agrárias, Londrina, 34(3), 1259–1272.

Wendt-Hornickle, E., & Snyder, L. B. (2016). Comparison of anesthesia with a morphine-lidocaine-ketamine infusion or a morphine-lidocaine epidural on time to extubation in dogs. Veterinary Anаesthesia and Analgesia, 43, 86–90.

How to Cite
Sliusarenko, D. V., IlnitskiyМ. G., Bilyi, D. D., Bobrytska, O. M., & Kovalova, L. O. (2021). Regional anesthesiological support for the implementation of surgical interventions in the abdomen of dogs . Regulatory Mechanisms in Biosystems, 12(1), 50-57.