Effect of immunological castration of male pigs on morphological and functional con-dition of the testicles

  • V. V. Samoilіuk Dnipro State Agrarian and Economic University
  • M. S. Koziy Petro Mohyla Black Sea National University
  • D. D. Bilyi Dnipro State Agrarian and Economic University
  • S. M. Maslikov Dnipro State Agrarian and Economic University
  • Т. L. Spitsina Dnipro State Agrarian and Economic University
  • L. I. Galuzina Dnipro State Agrarian and Economic University
Keywords: Improvak; testosterone level; Leydig cells; spermatogonia; spermatids; seminiferous tubules.

Abstract

Changes in the priorities of the treatment of animals in the conditions of intense technology of production of livestock products are based on the necessity of wellbeing of animals. Therefore, there is a need of search for and broad introduction of generally accepted alternatives to surgery, which would use modern means of castration, particularly immunocastration. The study presents morpho-functional substantiation of practicability of using immunological castration in the conditions of industrial production of pork. At the same time, we studied immunological castration using Improvak on the morphological and physiological condition of the testicles of male pigs. The testosterone level was determined using radioimmunologic method after 2, 4, 6, 8 weeks of immunological and surgical castrations, and also in intact boars of the control group. During the slaughter, we selected biopates of the testicles in immunological castrates and pigs of the control group for histological examination. The testosterone level 2 weeks after castration was the lowest in the animals castrated using Improvak. This indicator gradually increased, and after 8 weeks was higher than in the surgically castrated pigs. In the latter, the level of testosterone gradually decreased for 8 weeks, and did not significantly change in non-castrated pigs. The last stages of spermatogenesis in immunocastrates were inhibited after the second vaccination. As a result of immunological castration, the interstitial tissue of the testicle underwent changes. Between the tubules, a spreading of the loose connective tissue was observed. Leydig cells lost hyper chromaticity of the cytoplasm and typical polygonal profile, and their functional potential decreased. This fact was confirmed by the changes in the Hertwig’s ratio. In particular, we observed decrease in the value of the nuclear-cytoplasmic ratio. There were also a time shift of mitotic cycle, low degree of differentiation of spermatogonia and rupture of the course of the subsequent stages of spermatogenesis. However, there occurred multiplication of primary spermatogonia, single cellular divisions, and in the ductus deferentes, there could be found single spermatids. Some of them formed specific cellular groups of rounded and elliptic shapes in the seminiferous tubules. These structures were absent in the testicles of the control animals. Microstructural changes in the swine after injecting Improvak were characterized by deficiency of Leydig cells, indicating absence of the normal hormonal background, as confirmed by the results of the study of testosterone level. The epithelium-spermatogenic layer was underdeveloped, and the lumens of the tubules were in some places filled with generations of spermatocytes. In some places, meiosis was observed, which also indicates insignificant functioning of the testicles. Vaccination with Improvak caused atrophy of the testicles in swine and decrease in their functional condition, allowing it to be recommended it for broader application as an alternative to surgical castration.

References

Aluwé, M., Tuyttens, F. A. M., & Millet, S. (2015). Field experience with surgical castration with anaesthesia, analgesia, immunocastration and production of en tiremale pigs: Performance, carcass traits and boartaint prevalence. Animal, 9(3), 500–508.

Andersson, K., C. Brunius, С., Zamaratskaia, G., & Lundström, K. (2012). Early vaccination with Improvac®: Effects on performance and behaviour of male pigs. Аnimal Getaccess, 6(1), 87–95.

Bauer, А., Lacorn, M., & Claus, R. (2009). Effects of two levels of feed al locationon IGF‐I concentrations and metabolic parameters in GnRH‐immunized boars. Journal of Animal Physiology and Animal Nutrition, 93(6), 744–753.

Baumgartner, J., Laistera, S., Koller, M., Pfützner, A., Grodzycki, M., Andrews, S., & Schmoll, F. (2010). The behaviour of male fattening pigs followingei ther surgical castration or vaccination with a GnRF vaccine. Applied Animal Behaviour Science, 124, 28–34.

Bonneau, M., & Weiler, U. (2019). Pros and cons of alternatives to piglet castration: Welfare, boar taint, and other meat quality traits. Animals, 9(11), 884.

Bradford, J. R., & Mellencamp, M. A. (2013). Immunological control of boar taint and aggressive behavior in male swine. Animal Frontiers, 3(4), 12–19.

Brunius, C., Zamaratskaia, G., Andersson, K., Chen, G., Norrby, M., Madej, A., & Lundströma, K. (2011). Early immunocastration of male pigs with Improvac® – Effect on boar taint, hormones and reproductive organs. Vaccine, 29(51), 9514–9520.

Chen, M., Wang, J., Liu, N., Cui, W., Dong, W., Xing, B., & Pan, C. (2019). Pig SOX9: Expression profiles of Sertoli cell (SCs) and a functional 18 bpindel affecting testis weight. Theriogenology, 138(15), 94–101.

Claus, R., Lacorn, M., Danowski, K., Pearce, M. C., & Bauer, A., (2007). Short-term endocrine and metabolic reactions before and after second immunization against GnRH in boars. Vaccine, 25(24), 4689–4696.

Dunshea, F. R., Allison, J. R. D., Bertram, M., Boler, D. D., Brossard, L., Campbell, R., Crane, J. P, Hennessy, D. P., Huber, L., de Lange, C., Ferguson, N., Matzat, P., McKeith, F., Moreas, P. J. U., Mullan, B. P., Noblet, J., Quiniou, N., & Tokach, M. (2013). The effect of immunization against GnRF on nutrient re-quirements of male pigs: A review. Animal, 11(4), 643–649.

Einarsson, S., Andersson, K., Wallgrena, М., Lundström, К., & Rodriguez-Martinez, Н. (2009). Short- and long-term effects of immunization against gonadotropin-releasing hormone, using Improvac™, on sexual maturity, reproductive organs and sperm morphology in male pigs. Theriogenology, 71, 302–310.

Einarsson, S., Brunius, C., Wallgrena, M., Lundström, K., Andersson, K., Zamaratskaia, G., & Rodriguez-Martinez, H. (2011). Effects of early vaccination with Improvac® on the development and function of reproductive organs of male pigs. Animal Reproduction Science, 127, 50–55.

Fang, F., Li, H., Liu, Y., Zhang, Y., Tao, Y., Li, Y., Cao, H.,Wang, S., Wang, L., & Zhang, X. (2010). Active immunization with recombinant GnRH fusion protein in boars reduces both testicular development and mRNA expression levels of GnRH receptor in pituitary. Animal Reproduction Science, 119, 275–281.

Gogić, M., Radović, Č., Čandek-Potokar, M., Petrović, M., Radojković, D., Parunović, N., & Savić, R. (2019). Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs. Revista Brasileira de Zootecnia, 2019, 48.

Han, X., Cheng, W., Chen, Z., Du, X., Cao, X., & Zeng, X. (2014). Active immunisation against pregnenolone reduces testicular steroidogenesis and GnRH synthesis in rabbits. Animal Reproduction Science, 145, 161–169.

Han, X., Zhou, Y., Zeng, Y., Sui, F., Liu, Y., Tan, Y., Cao, X., Du, X., Meng, F., & Zeng, X. (2017). Effects of active immunization against GnRH versus surgical castration on hypothalamic-pituitary function in boars. Theriogenology, 97(15), 89–97.

Hilbe, M., Jaros, P., Ehrensperger, F., Zlinszky, K., Janett, F., Hässig, M., & Thun, R. (2006). Histomorphological and immunohistochemical findings in testes, bulbourethral glands and brain of immunologically castrated male piglets. Schweizer Archiv für Tierheilkunde, 148, 599–608.

Kress, К., Millet, S., Labussière, É., Weiler, U., & Stefanski, V. (2019). Sustainability of pork production with immunocastration in Europe. Sustainability, 11, 12.

Lugar, D. W., Rhoads, M. L., Clark-Deener, S. G., & Callahan, S. R. (2017). Immunological castration temporarily reduces testis size and function without long-term effects on libido and sperm quality in boars. Аnimal, 11(4), 643–649.

Mitjana, O., Bonastre, C., Tejedor, T., Garza, L., Latorred, Á., Morenob, B., & Falcetoa, V. (2020). Immuno-castration of female and male pigs with anti-gonadotrophin releasing hormone vaccine: Morphometric, histopathological and functional studies of the reproductive system. Animal Reproduction Science, 221, е106599.

Pasquale, J., Nannoni, E., Sardi, L., Rubini, G., Salvatore, R., Bartoli, L., Adinolfi, F., & Martelli, G. (2019). Towards the abandonment of surgical castrationin pigs: How is immunocastration perceived by Italian consumers? Animals, 9(5), 198.

Škrlep, M., Batorek, N., Bonneau, M., Prevolnik, M., Kubale, V., & Čandek-Potokar, M. (2012). Effect of immunocastration in group-housed commercial fattening pigs on reproductive organs, malodorous compounds, carcass and meat quality. Czech Journal of Animal Science, 57, 290–299.

Sladek, Z., Prudikova, M., Knoll, А., Kulich, P., Steinhauserova, I., & Borilova, G. (2018). Effect of early immunocastration on testicular histology. Veterinarnа Medicina, 63, 18–27.

Turkstra, J. A., Staay, F. J., Stockhofe-Zurwieden, N., Woelders, H., Meloen, R. H., & Schuurman, T. (2011). Pharmacological and toxicological assessment of a potential GnRH vaccine in young-adult male pigs. Vaccine, 29(21), 3791–3801.

Tuyttensa, F. A. M., Vanhonacker, F., Verhille, B., Brabander, D., & Verbeke, W. (2012). Pig producer at titude towards surgical castration of piglets without anaesthesia versus alternative strategies. Researchin Veterinary Science, 92(3), 524–530.

Wicks, N., Crouch, S., & Pearl, C. A. (2013). Effects of Improvac and Bopriva on the testicular function of boars ten weeks after immunization. Animal Reproduction Science, 142(3–4), 149–159.

Yu, S., Zhang, P., Dong, W., Zeng, W., & Pan, C. (2017). Identification of stem leydig cells derived from pig testicular interstitium. Stem Cells International, 2017, е2740272.

Yuna, J., Ollila, A., Valros, A., Larenzа-Menzies, Р., Heinonen, M., Oliviero, C., & Peltoniemi, O. (2019). Behaviour al alterations in piglets after surgical castration: Effects of analgesia and naesthesia. Researchin Veterinary Science, 125, 36–42.

Zamaratskaia, G., & Rasmussen, M. K. (2015). Immunocastration of Male Pigs – Situation Today. Procedia Foodcience, 5, 324–327.

Zamaratskaia, G., Rydhmer, L., Andersson, K., Chen, G., Lowagie, S., Andersson, K., & Lundström., K. (2007). Long-term effect of vaccination against gonado-tropin-releasing hormone, using Improvac™, on hormonal profile and behaviour of male pigs. Animal Reproduction Science, 108, 37–48.

Published
2021-02-18
How to Cite
SamoilіukV. V., Koziy, M. S., Bilyi, D. D., Maslikov, S. M., SpitsinaТ. L., & Galuzina, L. I. (2021). Effect of immunological castration of male pigs on morphological and functional con-dition of the testicles . Regulatory Mechanisms in Biosystems, 12(1), 20-26. https://doi.org/10.15421/022104