Morphology of spinal ganglia of different segmentary levels in the domestic dog
AbstractThe spinal ganglia, which perform the function of the first link on the afferent impulses’ way from the receptors to the central nervous system, recognize internal and external irritations, and are the first to transform them into a nervous impulse. As the representatives of the peripheral nervous system, they are some of the main objects of the studies in contemporary neuromorphology. Based on the results of anatomic, neurohistological, histochemical, morphometric and statistical methods of the studies, we conducted a complex survey, revealing the morphology of spinal ganglia of different segmental levels in the domestic dog. In particular, we determined the differences in the microscopic structure and morphometric parameters of cervical, thoracic, lumbar and sacral spinal ganglia and the ganglia of the cervical and lumbar enlargements in mature domestic dogs. The study showed that the spinal ganglia of domestic dogs can have different skeletotopy, different shape and sizes due to their species peculiarity. Also, the surveyed animals, according to the results of our studies, had the cervical and thoracic spinal ganglia of oval, while the lumbar and sacral – spindle-like shapes. According to the results of morphometry, the area of the spinal ganglia in lengthwise section differed: the smallest area belonged to the thoracic, the largest to the sacral spinal ganglia. The density of neuronal arrangement per 0.1 mm2 of the area of the spinal ganglia correlated with their sizes: the highest parameter was identified for the thoracic spinal ganglia, the lowest – for the sacral. The conducted studies revealed that histo- and cyto-structure of the spinal ganglia is characteristic of notable differentiation of the nervous cells of small sizes. Therefore, we differentiated neurons of the spinal ganglia into large, medium and small. The highest quantity of large neurons was found in the sacral ganglia, and largest amount of medium-sized neurons – in the ganglia of the lumbar enlargement. In other ganglia, small neurons dominated. Correspondingly, different nuclear-cytoplasmic ratio in these neurons was determined, indicating different extent of morphofunctional condition of nervous cells. We determined content of localization and separation of nucleic acids in histostructure of the spinal cord at the tissue and cellular levels.
Avtandilov, G. G. (1990). Medicinskaja morfometrija [Medical morphometry]. Medicina, Moscow (in Russian).
Bahar, S., Eken, E., & Sur, E. (2006). The morphology of the hypoglossal dorsal root and its ganglia in Holstein cattle. Journal of Veterinary Medical Science, 68(6), 533–536.
Bailey, F. R. (1901). Studies on the morphopogy of ganglion cells in the rabbit: I. The normal nerve Cells. II. Changes in the nerve cells in rabbies. The Journal of Experimental Medicine, 5(6), 549.
Citkowitz, E., & Holtzman, E. (1973). Peroxisomes in dorsal root ganglia. Journal of Histochemistry and Cytochemistry, 21(1), 34–41.
Goralskiy, L. P., Guralska, S. V., Kolesnik, N. L., & Sokulskiy, I. M. (2018). The features of the structural organization of spinal ganglia in a subphylum of vertebtates. Vestnik Zoologii, 52(6), 501–508.
Hamzianpour, N., Eley, T. S., Kenny, P. J., Sanchez, R. F., Volk, H. A., & De Decker, S. (2015). Magnetic resonance imaging findings in a dog with sensory neuronopathy. Journal of Veterinary Internal Medicine, 29(5), 1381.
Hanani, M. (2005). Satellite glial cells in sensory ganglia: From form to function. Brain Research Reviews, 48(3), 457–476.
Hirose, G., & Jacobson, M. (1979). Clonal organization of the central nervous system of the frog: I. Clones stemming from individual blastomeres of the 16-cell and earlier stages. Developmental Biology, 71(2), 191–202.
Horalskyi, L. P., Khomych, T. V., & Kononskyi, O. I. (2015). Osnovy histolohichnoji tekhniky i morfofunktsionalni metody doslidzhennia u normi ta pry patolohiji [Basics of histological technique and morphofunctional methods of research in normal and pathology]. Polissia, Zhytomyr [in Ukrainian].
Kikuchi, S., Sato, K., Konno, S., & Hasue, M. (1994). Anatomic and radiographic study of dorsal root ganglia. Spine, 19(1), 6–11.
Kobayashi, S., Mwaka, E. S., Baba, H., Kokubo, Y., Yayama, T., Kubota, M., Nakajama, H., & Meir, A. (2010). Microvascular system of the lumbar dorsal root ganglia in rats. Part II: neurogenic control of intraganglionic blood flow. Journal of Neurosurgery: Spine, 12(2), 203–209.
Larnicol, N., Rose, D., & Duron, B. (1988). Morphometrical study of the cat thoracic dorsal root ganglion cells in relation to muscular and cutaneous afferent innervation. Neuroscience Research, 6(2), 149–161.
Lazriev, I. L., Kostenko, N. A., & Lordkipanidze, T. G. (2001). Distribution of neuro-and macrogliocytes in layers in different parts of the auditory cortex of the cat brain (quantitative studies). Neuroscience and Behavioral Physiology, 31(6), 613–616.
Lobko, P. I., Kovaleva, D. V., Koval’chuk, I. E., Pivchenko, P. G., Rudenok, V. V., & Davydova, L. A. (2000). Information analysis of spinal ganglia. Morfologija, 118(4), 36–40.
Pannese, E., Bianchi, R., Calligaris, B., Ventura, R., & Weibel, E. R. (1972). Quantitative relationships between nerve and satellite cells in spinal ganglia. An electron microscopical study. I. Mammals. Brain Research, 46, 215–234.
Polak, M. (1965). Morphological and functional characteristics of the central and peripheral neuroglia (light microscopical observations). Progress in Brain Research, 15, 12–34.
Rubinow, M. J., & Juraska, J. M. (2009). Neuron and glia numbers in the basolateral nucleus of the amygdala from preweaning through old age in male and female rats: A stereological study. Journal of Comparative Neurology, 512(6), 717–725.
Russo, D., Clavenzani, P., Mazzoni, M., Chiocchetti, R., Di Guardo, G., & Lalatta‐Costerbosa, G. (2010). Immunohistochemical characterization of TH13‐L2 spinal ganglia neurons in sheep (Ovis aries). Microscopy Research and Technique, 73(2), 128–139.
Safonova, G. D., & Kovalenko, A. P. (2006). Morphofunctional characteristics of neurons in the spinal ganglia of the dog in the post-distraction period. Neuroscience and Behavioral Physiology, 36(5), 491–494.
Schiønning, J. D., & Larsen, J. O. (1997). A stereological study of dorsal root ganglion cells and nerve root fibers from rats treated with inorganic mercury. Acta Neuropathologica, 94(3), 280–286.
Schröder, H., Moser, N., & Huggenberger, S. (2020). Basic neurohistology. In: Neuroanatomy of the mouse. Springer, Cham. Pp. 7–25.
Sharpey-Schafer, E. A. (1881). VI. Note on the occurrence of ganglion cells in the anterior roots of the cat’s spinal nerves. Proceedings of the Royal Society of London, 31(206–211), 348.
Stepanchuk, A. P. (2020). Morphology and function of the autonomous nervous system. Aktualni Problemy Suchasnoi Medytsyny, 20(1), 212–217.
Tandrup, T. (1993). A method for unbiased and efficient estimation of number and mean volume of specified neuron subtypes in rat dorsal root ganglion. Journal of Comparative Neurology, 329(2), 269–276.
Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgärtner, W., & Gerhauser, I. (2017). Canine dorsal root ganglia satellite glial cells represent an exceptional cell population with astrocytic and oligodendrocytic properties. Scientific reports, 7(1), 1–15.
Topp, K. S., Tanner, K. D., & Levine, J. D. (2000). Damage to the cytoskeleton of large diameter sensory neurons and myelinated axons in vincristine‐induced painful peripheral neuropathy in the rat. Journal of Comparative Neurology, 424(4), 563–576.
Yun, S., Kim, W., Kang, M. S., Kim, T. H., Kim, Y., Ahn, J. O., & Chung, J. Y. (2020). Neuropathological changes in dorsal root ganglia induced by pyridoxine in dogs. BMC Neuroscience, 21(1), 1–9.
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