Physiological relationship between content of certain microelements in the tissues of different anatomic sections of the organism of honey bees exposed to citrates of argentum and cuprum

  • I. Kovalchuk Institute of Anomal Biology, National Academy of Agrarian Sciences of Ukraine
  • I. Dvylyuk Institute of Anomal Biology, National Academy of Agrarian Sciences of Ukraine
  • Y. Lecyk Institute of Anomal Biology, National Academy of Agrarian Sciences of Ukraine
  • I. Dvylyuk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • B. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
Keywords: zinc; lead; cadmium; ferrum; the tissues of head; abdominal and thorax section

Abstract

The study was conducted on honey bees of the Carpathian breed on the basis of the apiary of S. Z. Gzhytskyi Lviv National University of Veterinary Medicine and Biotechnologies. The bees were kept in 8-frame beehives with size of the frame of 435 × 300 mm. The study was undertaken in the summer-autumn period. A total of 5 groups of bee families were formed according to the analogue principle, with three families of bees in each group. Control (I) group received sugar syrup in 1 : 1 concentration (1000 mL/group/week). In the experimental (II) group, Ag was added to the syrup in amount of 0.5 mL/g of the sugar syrup; in III experimental group – in similar conditions Ag was added in 1 mg/L amount; IV group received sugar syrup with Cu in 0.5 mg/L amount; V experimental group – Cu in amount of 1 mg/L of sugar syrup. Microelements were added to sugar syrup in the form of citrates obtained using method of nanotechnology, developed by the company Nanomaterials and Nanotechnologies in Kyiv. The period of monitoring lasted 28 days with 7 days interval of preparation. Inter-group and anatomic differences were found in content of certain mineral elements in the tissues of the head, thorax and abdominal sections of the honey bees in conditions of feeding with citrates of argentum and cuprum in sugar syrups. Under the influence of citrate of argentum in amount of 0.5 and 1.0 mg/L of sugar syrup, content of ferrum increased in the tissues of the head and abdominal sections, though it decreased in the thorax, content of cuprum decreased in the tissues of head, thorax and abdomen, content of zinc increased in the tissues of head and thorax, though it decreased in the abdomen, content of lead and cadmium reduced in the tissues of all anatomic sections of the organism of honey bees. Under the influence of cuprum in amount of 0.5 and 1.0 mg/L of sugar syrup, content of ferrum reduced in the tissues of the head, though it increased in the tissues of thorax and abdomen, content of cuprum increased, and the content of zinc, lead and cadmium reduced in the tissues of all anatomic sections of honey bees. According to the results of the study, the determining effect of feeding of honey bees on the optimization of the parameters of mineral metabolism in different anatomic sections of the organism of bees is discussed.

References

Anderson, R. (1987). Chromium. In: Mertz, M. (Ed.). Trace elements in human and animal nutrition. Academic Press, San Diego. Pp. 225–244.

Anton, A., Mathe, P., Radimzky, L., Füleky, G., & Biczók, G. (1994). Effects of environmental factors and Mn, Zn, Cu trace elements on the soil phosphormonoes terase and amidase activity. Acta Biologica Hungarica, 45(1), 39–50.

Baker, A., Harvey, L., Majask-Newman, G., Fairweather-Tait, S., Flynn, A., & Cashman, K. (1999). Effect of dietary copper intakes on biochemical marcers of bone metabolism in healthy adult males. European Journal of Clinical Nutrition, 53(5), 408–412.

Bogdanov, S., Haldimann, M., Luginbuhl, W., & Gallmann, P. (2007). Minerals in honey: Environmental, geographical and botanical aspects. Jornal of Apicultural Research and Bee World, 46(4), 269–275.

Borysevuch, V. B., Kaplynenko, V. G., & Kosinov, N. V. (2012). Nanomatarialy i nanotechnologii v veterynarnoy practike [Nanomaterials and nanotechnologies in veterinary practice]. VD Avitsena, Kyiv (in Russian).

Brodschneider, R., & Crailsheim, K. (2010). Nutrition and health in honey bees. Apidologie, 41(3), 278–294.

Chekman, I. S. (2011) Nanofarmakolohiya [Nanopharmacology]. Zadruha, Kyiv (in Ukrainian).

Conti, M. E., & Botre, F. (2001). Honey bees and their products as potential bioindicators of heavy metals contamination. Environmental Monitoring and Assessment, 69(3), 267–282.

Dolaychuk, О. P., Fedoruk, R. S., Kovalchuk, I. I., & Kropyvka, S. I. (2015). Physiological and biochemical processes in the organisms of rats when feeding them with different amounts of germanium citrate. The Animal Biology, 17(2), 50–56.

Finch, C. A., & Huebers, H. (1982). Perspectives in iron metabolism. The New England Journal of Medicine, 306, 1520–1528.

Harris, E. D. (2000). Cellular copper transport and metabolism. Annual Review of Nutrition, 20, 291–310.

Iskra, R. Y., Vlizlo, V. V., Fedoruk, R. S., & Antonyak, G. L. (2014). Crom u zhyvlenni tvaryn [Chrome in animal nutrition]. Ahrarna Nauka, Kyiv (in Ukrainian).

Kabata-Pendias, A. (2011). Trace elements in soils and plants. 4th edition. CRC Press, Boca Raton.

Kovalchuk, I. I., & Fedoruk, R. S. (2008). Melliferous bees and honey are bioindicator contamination of environment by heavy metals. The Animal Biology, 10(1–2), 24–32.

Kovalskyi, Y., Gucol, A., Gutyj, B., Sobolev, O., Kovalska, L., & Mironovych, A. (2018). Features of histolism and hystogenesis in the vital temperature range in the organism of honey bee (Apis mellifera L.) in the postembrional period. Ukrainian Journal of Ecology, 8(2), 301–307.

Lebedev, V., & Bilash, N. (1991). Biologija medonosnoj pchely [Honey bee biology]. Agopromizdat, Moscow (in Russian).

Mazo, V. K., & Shyrina, L. I. (2005). Medyi v pitanii cheloveka: Vsacyvanie i biodostypnost [Copper in human nutrition: Absorption and bioavailability]. Voprocy Pitaniya, 74(2), 52–59 (in Russian).

McDowell, L. R. (2003). Minerals in animal and human nutrition. Elsevier Health Sciences.

Nasolodin, V. V., Dvorkin, V. A., & Kurkova, S. D. (1994). Biodostypnost microelementov i vzaemodeystvie ih v prozesse obmena v organizme [The bioavailability of trace elements and their interaction in the process of exchange in the body]. Gigiena i Sanitariya, 9, 12–14 (in Russian).

Terzic, L., Terzic, V., Krunic, M., & Brajkovic, M. (1984). Honey bee poisoning caused by arsenic from copper smelter smoke. Acta Veterinaria, 34(1), 54–62.

Tyshhenko, V. (1986). Fiziologija nasekomyh [Physiology of insects]. Vysshaja Shkola, Moscow (in Russian).

Vincent, J. B. (2007). The nutritional biochemistry of chromium (III). Department of Chemistry the University of Alabama Tuscaloosa, USA.

Vishchur, V. Y., Saranchuk, I. I., & Gutyj, B. V. (2016). Fatty acid content of honeycombs depending on the level of technogenic loading on the environment. Visnyk of Dnipropetrovsk University, Biology, Ecology, 24(1), 182–187.

Published
2019-05-27
How to Cite
Kovalchuk, I., Dvylyuk, I., Lecyk, Y., Dvylyuk, I., & Gutyj, B. (2019). Physiological relationship between content of certain microelements in the tissues of different anatomic sections of the organism of honey bees exposed to citrates of argentum and cuprum . Regulatory Mechanisms in Biosystems, 10(2), 177-181. https://doi.org/10.15421/021926