Comparative evaluation of bone marrow cells morpho-functional activity in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors of the first and second generation

I. O. Zhaleyko; D. I. Bilko; I. S. Dyagil; N. M. Bilko

doi:10.15421/021420

I. O. Zhaleyko Centre for Molecular and Cell Research of the National University of Kyiv-Mohyla Academy
D. I. Bilko Centre for Molecular and Cell Research of the National University of Kyiv-Mohyla Academy
I. S. Dyagil Scientific Centre of the Radiation Medicine NAMS of Ukraine
N. M. Bilko Centre for Molecular and Cell Research of the National University of Kyiv-Mohyla Academy

Keywords: chronic myeloid leukemia, cell culture in vitro, tyrosine kinase inhibitors

Abstract

The efficiency of using the culture techniques of research for monitoring the patient’s response to the treatment by tyrosine kinase inhibitors of the first and second generation is shown. Thus, the functional activity of bone marrow cells in patients having the optimal treatment response to inhibitors of tyrosine kinases was significantly lower compared with patients with the acquired resistance to the drug, and patients who had CML diagnosed for first time. Furthermore, for patients with the optimal response to the nilotinib therapy, numbers of colonies in semi-solid agar in vitro was lower, than in patients with the optimal response to imatinib. When the leukaemic cell clone becomes resistant to tyrosine kinase inhibitors, the prevalence of early cells of granulocyte-macrophage hematopoietic stem cells is observed in CFU culture which can be an important prognostic factor for choosing the appropriate treatment strategy.

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