Phage type and sensitivity to antibiotics of Staphylococcus aureus film-forming strains isolated from airway mucosa
AbstractToday film-forming strains of bacteria play very important role in clinical pathology. Staphylococci are ones of most dangerous of them. This bacteria can determine different pathological processes, for example, complication of airway mucosa. The ability to form a biofilm is one of the main properties of nosocomial strains. These strains should be monitored and their carriers are to be properly treated. To determine the origin of staphylococci strains we used bacteriophages from the International kit. The aim of research was to determine the phage type of staphylococci film-forming strains, that were isolated from naso-pharingial mucosa. Phage typing has been carried out for 16 film-forming strains of S. aureus. To solve this problem, we used the International phage kit by Fisher’s method. As a result, sensitivity to phages from the International kit showed 53.8% of studied strains of S. aureus. 64.3% of sensitivity strains were lysed by one of the phage, 21.4% – were by two of the phages, 14.3% – by three of the phages. Isolates were sensitive to phages: 81 – 42.9%, 75 – 35.7%, 28.6% were sensitive to phages 47 and 53. All cases of detection of sensitivity to phage 47 coincided with the ability to form biofilm. Among non-film-forming strains there was no sensitive strains for this phage. Film-forming strains resist to erythromycin (62.5%), ciprofloxacin (43.8%), gentamicin (56.3%), tetracycline (87.5%), amoxicillin (93.8%), and cefuroxime (37.5%). All cases of sensitivity to phage 47 coincided with resistance to erythromycin, amoxicillin and tetracycline. For two of these strains, we also defined resistance to gentamicin and for one of them – to ciprofloxacin. Results of research allowed to relate the bacterial cultures for determining the type. This may have implications for studying of film-forming ability, because surface structures of bacterial cell take place in this process. Belonging of an isolate to specific phage type may indicate possible differences in the structure of the cell wall, composition of the cytoplasmic membrane, receptors, etc. So, phage typing may be used as one of tests to study film-forming processes. Besides, phage typing can be helpful in finding of the source and modes of transmission of nosocomial strains of bacteria. Given the increased level of antibiotic resistance among film-forming strains of staphylococci, the importance of this monitoring is indisputable, because spreading of these strains may cause the failure of therapy.
Arunava, K., Selvaraj, S., Umadevi, S., Shailesh, K., Noyal, M.., Sreenivasan, S., Joshy, M.E., 2013. Bacteriophage types of methicillin-resistant Staphylococcus aureus in a tertiary care hospital. Australas. Med. J. 6(10), 496–503.
Blair, J.E., Williams, R.E.O., 1961. Phage typing of staphylococci. Bull WHO 24, 771–778.
Bondarenko, V.М., 2011. Rol uslovno-patogennih bakterii pri chronicheskih vospalitelnih processah razlichnoi lokalizacii [The role of opportunistic bacteria in chronic inflammatory processes of different localization]. Triada, Twer (in Russian).
Donlan, R.M., Costerton, J.W., 2002. Biofilms: Survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 15(2), 167–193. >>doi: 10.1128/CMR.15.2.167-193.2002
Dzahurov, S.H., Rezepov, F.F. (Eds.), 1995. Spravochnik po primrneniiu bakteriinih i virusnih preparatov [Handbook of bacterial and viral preparations application]. Medicine, Moscow (in Russian).
Ekmuzheva, D.Z., Khadzegova, S.B., 2003. Kharakteristika normalnoy mikroflory vlagalischa u zdorovykh zhenschin reproduktivnigo vozrasta [Characteristic of the normal vaginal microflora in healthy women of reproductive age]. Uspekhi Sovremennogo Estestvoznaniya 6, 50–51.
Falova, O.E., Potaturkina-Nesterova, N.I., Ilina, E.N., 2013. Vzaimosviaz vnutrividovogo raznoobraziia i geneticheskih determinant patogennosti stafilokokkov kozhi [Interrelation of intraspecific variety and genetic determinants of skin’s staphylococcus pathogenicity]. Fundamental Research 12, 131–134 (in Russian).
Fujisawa, H., Ishihara, K., 1968. Penicillin resistance correlated with phage types in Staphylococcus aureus. Japan. J. Microbiol. 12(1), 1–6. >>doi: 10.1111/j.1348-0421.1968.tb00363.x
Holt, J.G., Кrieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T. (Eds.), 1994. Bergey’s manual of determinative bacteriology. Williams & Wilkins, Baltimore.
Ilyina, Т.S., Romanova, Y.M, Hinzburh, А.L., 2004. Bioplenki kak sposob sushestvovaniia bakterii v okruzhaiushei srede i orhanisme hoziaina: Fenomen, heneticheskii control i sistemi rehuliacii ih razvitiia [Biofilms as a way of existence of bacteria in the environment and the host organism: The phenomenon, the genetic control system and the regulation of their development]. Genetic. 40, 1–12 (in Russian).
Kaimal, S., D'Souza, M., Sistla, S., Parija, S.C., 2012. Phage typing in dermatitis cruris pustulosa et atrophicans: Does staphylococcal carrier status have a role? Int. J. Dermatol. 51(11), 1335–1339. >>doi: 10.1111/j.1365-4632.2011.05438.x
Kali, A., Stephen, S., Sivaraman, U., Kumar, S., Joseph, N.M., Srirangaraj, S., Easow, J.M., 2013. Bacteriophage types of methicillin-resistant Staphylococcus aureus in a tertiary care hospital. Australas. Med. J. 6(10), 496–503. >>doi: 10.4066/AMJ.2013.1742
Labinskaia, А.S., Volina, Y.H. (Eds.), 2008. Rukovodstvo po medicinskoi mikrobiolohii. Obshaia i sanitarnaia mikrobiolohiia [Manual of medical microbiology. General and sanitary microbiology]. Binom, Мoscow (in Russian).
Mehndiratta, P.L., Gur, R., Saini, S., Bhalla, P., 2010. Staphylococcus aureus phage types and their correlation to antibiotic resistance. Indian J. Pathol. Microbiol. 53(4), 738–741. >>doi: 10.4103/0377-4929.72065
Murchan, S., Carter, M., 2000. Strain identities of phage non-typable MRSA in the UK. J. Hosp. Infect. 46, 157–158. >>doi: 10.1053/jhin.2000.0826
Ob unifikacii mikrobiolohicheskih (bakteriolohicheskih) metodov issledovaniia, primeniaemih v kliniko-diahnosticheskih laboratoriiah lechebno-profilacticheskih ucherezhdenii, 1985 [About the unification of microbiological (bacteriological) research methods used in clinical diagnostic laboratories of medical institutions: the order № 535 (1985)]. MOZ USSR, Moscow (in Russian).
Olson, M.E., Ceri, H., Morck, D.W., Buret, A.G., Read, R.R., 2002. Biofilm bacteria: Formation and compararive susceptibility of antibiotics. Can. J. Vet. Res. 66(2), 86–92.
Pantucek, R., Doskar, J., Ruzickova, V., Kasparek, P., Oracova, E., Kvardova, V., Rosypal, S., 2004. Identification of bacteriophage types and their carriage in Staphylococcus aureus. Arch. Virol. 149, 1689–1703. >>doi: 10.1007/s00705-004-0335-6
Paul-Satyaseela, M., Belkum, A., Shivannavar, C.T., Gaddad, S.M., 2011. Comparison of capsular typing of Staphylococcus aureus with bacteriophage typing: A study in Gulbarga, India. Indian J. Med. Microbiol. 51(3), 359–362. >>doi: 10.1007/s12088-011-0108-3
Pro zatverdzhennia metodichnih vkazivok schodo viznachennya chutlivostі mіkroorganіzmіv do antibakterіalnih preparatіv, 2007 [Approval of guidelines for determining the sensitivity of microorganisms to antibiotics. The order № 167 (2007)]. Ministry of Health of Ukraine, Kyiv (in Ukrainian).
Savchuk, Т.D., 2003. Stafilokokkovaia infekciia [Staphylococcal infections]. Medicine, Moscow (in Russian).
Volkov, I.I., 1999. Sovershenstvovanie mikrobiolohicheskoi diahnostiki stafilokokkovih infekcii i ekolohicheskie aspekti ih vozbuditelei [Improving the microbiological diagnosis of staphylococcal infections and environmental aspects of their pathogens] Autoref. of candidate dissertation. Available from http://nature.web.ru/db/msg.html?mid=1163020.
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