Frequency of urogenital mycoplasma detection in women of Dnipropetrovsk

  • K. V. Bubalo Oles Honchar Dnipropetrovsk National University Diagnostic Center of Dnipropetrovsk Medical Academy
  • L. P. Golodok Oles Honchar Dnipropetrovsk National University
  • A. I. Vinnikov Oles Honchar Dnipropetrovsk National University
Keywords: DUO test-system, PCR, Ureaplasma urealyticum, Мycoplasma hominis, urogenital tract


The frequency of urogenital mycoplasmas detection in women of different ages was studied in culture with the help of DUO test-system in order to determine their etiological significance in the development of inflammatory processes of women urogenital tract. We identified the researched cultures Mycoplasma hominis, Ureaplasma urealyticum in the diagnostic titer >104 TEM/ml indicating severe contamination by microorganisms, and in the titer <103 TEM/ml, the carrier state of the identified microorganisms. Of 120 studied isolates of women urogenital tract there have been identified 113 strains of genital mycoplasmas, among which 63%  – U. urealyticum, 32% – M. hominis, 3% – microbial association of U. urealyticum – M. hominis. According to the study of frequency of detection of urogenital mycoplasma using DUO test-system culture method, it was found that the most frequently observed ones were U. urealyticum in 75 women (63%) of all individuals, M. hominis in 38 women (32%) in different diagnostic titers (>104 TEM/ml, <103 TEM/ml) in 4 women (3%) U. urealyticum – M. hominis was observed in microbial associations and mycoplasma were not found in 3 women (2%) of all surveyed patients. U. urealyticum and M. hominis in the diagnostic titer of >104 TEM/ml was observed in 55 women (46%) and 20 women (17%), respectively, and the titer of <103 CFU/ml U. urealyticum was observed in 20 women (17%), and M. hominis in 18 women (15%). Analysis of genital mycoplasmas distribution among women of different ages has shown that there was the certain correlation between the patient age and frequency of genital mycoplasmas detection: the highest detection rate was observed in women age of 24–29. The dominant pathogen of urogenital tract inflammatory processes in women in 24–29 age group is U. urealyticum. The comparison of DUO test-system and PCR data has shown that DUO test-system in culture allowed more sensitive quantitave characterization of mycoplasmas, however, for the more effective laboratory diagnostics it was necessary to use complex methods to increase the probability of pathogen detection. Incidence of mycoplasmas in women with the presence of inflammation was higher than in women having the inflammation in the genital tract. In this case, potential symptom-free carriers exist for the development of inflammation of urogenital tract of women. Scientists have proved that mycoplasma could cause vulvovaginitis, urethritis, paraurethritis, bartholinitis, adnexitis, salpingitis, endometritis, and ovaritis. 


Aujard, Y., Maury, L., Doit, C., 2005. Ureaplasma urealyticum and Mycoplasma hominis infections in newborns: Personal data and review of the literature. Arch. Pediatr. 12(1), 12–18.

Bashmakova, M.A., 2006. Laboratory diagnosis of genital infections. Reproduct. Рroblem. 5(1), 25–28 (in Russian).

Boesen, Т., Emmersen, J., Baczynska, А., 2004. The vaa locus of Mycoplasma hominis contains a divergent genetic islet encoding a putative membrane protein. BMC Microbiol. 4(1), 37–38.

Deguchi, T., Yoshida, T., Miyazawa, T., 2004. Association of Ureaplasma urealyticum (biovar 2) with nongonococcal urethritis. Sex. Transm. Dis. 31(3), 192–195.

Jensen, A., Kleveland, C., Moghaddam, A., 2013. Chlamydia trachomatis, Mycoplasma genitalium and Ureaplasma urealyticum among students in northern Norway. J. Eur. Acad. Dermatol. Venereol. 27(1), 146–147.

Karabay, O., Topcuoglu, A., Kocoglu, E., 2006. Prevalence and antibiotic susceptibility of genital Mycoplasma hominis and Ureaplasma urealyticum in a university hospital in Turkey. Clin. Exp. Obstet. Gynecol. 33(1), 36–38.

LeRoy, C., LeHen, I., Clerc M., Arfel, K., 2012. The first performance report for the Bio–Rad Dx CT/NG/MG assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urogenital samples. J. Microbiol. Methods. 89(3), 193–197.

Lypova, E.V., Batakaev, E.V., 2009. Urogenital systems of associated infection with Mycoplasmas. Clin. Dermatol. Venereol. 26, 455–460.

Pereyre, S., Sirand-Pugnet, P., Beven, L., 2009. Life on arginine for Mycoplasma hominis: Clues from its minimal genome and comparison with other human urogenital mycoplasmas. Plos. Genet. 10(5), 367–368.

Petrikkos, G.L., Hadjisoteriou, M., Daikos, G.L., 2007. PCR versus culture in the detection of vaginal Ureaplasma urealyticum and Mycoplasma hominis. Int. J. Gynaecol. Obstet. 97(3), 202–203.

Rivera, J.A., Centeno, M., Santellan, O.M., Rodríguez, N.M., 2004. Prevalencia de Ureaplasma urealyticum en mujeres. Rev. Mex. Patol. Clin. 51(1), 33–36 (in Spanish).

Rivera, J.A., Cedillo, M.L., Vega, M., 2001. Micoplasmasy su importancia médica. Rev. Biomed. 12(4), 262–271 (in Spanish).

Robertson, J.A., Stemke, G.W., Davis, J.W., 2002. Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum. Int. J. Syst. Evol. Microbiol. 52(2), 587–597.

Ross, J.D., 2005. Is Mycoplasma genitalium a cause of pelvic inflammatory disease? Infect. Dis. Clin. North. Am. 19(2), 407–408.

Taylor-Robinson, D., 2007. The role of mycoplasmam in pregnancy outcome. Best. Pract. Res. Clin. Obstet. Gynaecol. 21(3), 425-438.

Taylor-Robinson, D., Jensen, J.S., Svenstrup, H., Stacey, C.M., 2012. Difficulties experienced in defining the microbial cause of pelvic inflammatory disease. Int. J. STD AIDS 23(1), 18–24.

Zdrodowska-Stefanow, B., Kosowska, W.M., OstaszewskaPuchalska, I., 2006. Ureaplasma urealyticum and Mycoplasma hominis infection in women with urogenital diseases. Adv. Med. Scien. 51(1), 250–253.

Zuo, C.X, Huang, J.H., Chen, J., Lu, J.Y., Xiang, Y.P., 2006. [Female urogenital mycoplasma infection and drug sensitivity status in Changsha]. Nan. Fang. Yi. Ke. Da. Xue. Xue. Bao. 26(6), 831–836 (in Chinese).

How to Cite
Bubalo, K. V., Golodok, L. P., & Vinnikov, A. I. (2014). Frequency of urogenital mycoplasma detection in women of Dnipropetrovsk. Regulatory Mechanisms in Biosystems, 5(1), 45-48.