Culural parameters effect of biosynthesis of proteolytic enzyme complexes from Yarrowia lipolytica

  • A. M. Goncharov Oles’ Gonchar Dnipropetrovsk National University
  • V. G. Gavriljuk Oles’ Gonchar Dnipropetrovsk National University
  • E. V. Matseljukh Oles’ Gonchar Dnipropetrovsk National University
  • A. I. Vinnikov Oles’ Gonchar Dnipropetrovsk National University

Abstract

It was established, that production of proteolytic enzymes from yeasts is effected by sources of Carbon, Nitrogen and energy. The impact of different organic substances to proteo-, fibrino-, gelatine- and haemoglobinolytic enzyme activity from Yarrowia lipoytica 2061 is established. Positive effect of addition galactose and gelatin with glycine in cultural liquid is revealed. Optimal temperature, pH and other physical parameters for getting proteolityc enzyme complexes is defined.

References

Влияние координационных соединений германия на активность ряда гликозидаз / Л. Д. Варбанец, О. Н. Рзаева, Е. В. Мишак и др. // Мікробіол. журн. – 2007. – Т. 69, № 3. – С. 11–18.

Долгих М. С. Протеолитическая активность дрожжеподобных грибов рода Candida – продуцентов белка / М. С. Долгих, Э. Г. Кравцов, А. В. Ермолаев // Микология и фитопатология. – 1990. – Т. 24, № 3. – С. 229–235.

Петрова И. С. Определение протеолитической активности ферментных препаратов микробного происхождения / И. С. Петрова, М. Н. Винцюнайте // Прикл. биохимия и микробиология. – 1966. – Т. 2, № 1. – С. 322–327.

Полыгалина Г. В. Определение активности ферментов / Г. В. Полыгалина, В. С. Чередниченко, Л. В. Римарева. – М. : ДеЛипринт, 2003. – 250 с.

Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration // Biochem. J. – 1964. – Vol. 91, N 2. – P. 222–232. >>doi:10.1042/bj0910222

Balaraman K. Production and purification of fibrinolytic enzyme (trombinase) from Yarrowia lipolytica / K. Balaraman, G. Prabokaran // Indian J. Med. Res. – 2007. – Vol. 126, N 5. – P. 459–464.

Barett A. J. Proteolytic enzymes: aspartic and metalloproteases // Meth. Enzymol. – 1995. – Vol. 248. – P. 183–197.

Genetic control of extracellular protease synthesis in the yeast Yarrowia lipolytica / C. I. Gonzalez-Lopez, R. Szabo, S. Blanchin-Rolanda, C. Gaillardina // Genetics. – 2002. – Vol. 160. – P. 417–427.

Growth and proteolytic activity of hairy roots from Yarrowia lipolytica: effect of nitrogen and sucrose / P. M. L. Lourenço, S. de Castro, T. M. Martins et al. // Enzyme and Microbial Technology. – 2002. – Vol. 31, N 3. – P. 242–249.

pH-regulated expression of the acid and alkaline extracellular proteases of Yarrowia lipolytica / D. J. Glover, R. K. McEven, C. R. Thomas, T. W. Young // Microbiology. – 1997. – Vol. 143. – P. 3045–3054. >>doi:10.1099/00221287-143-9-3045

The intracellular proteolytic system of Yarrowia lipolytica and characterization of an aminopeptidase / Z. Hernández-Montañez, J. Araujo-Osorio, Y. Noriega-Reyes et al. // FEMS Microbiol. Lett. – 2007. – Vol. 268, N 2. – Р. 178–186.

Protein measurement with the folin phenol reagent / O. H. Lowry, N. J. Rosebrough, A. L. Farr, R. J. Randall // J. Biol. Chem. – 1951. – Vol. 193, N 1. – P. 265–275.

Protein expression and secretion in the yeast Yarrowia lipolytica / J. M. Nicaud, C. Madzak, P. van den Broek et al. // FEMS Yeast Res. – 2002. – Vol. 2, N 3. – P. 371–379.

Published
2010-06-05
How to Cite
Goncharov, A. M., Gavriljuk, V. G., Matseljukh, E. V., & Vinnikov, A. I. (2010). Culural parameters effect of biosynthesis of proteolytic enzyme complexes from Yarrowia lipolytica. Regulatory Mechanisms in Biosystems, 1(1), 50-54. https://doi.org/10.15421/021008