Age-related characteristics of spectral bioelectric activity of the trophotropic zone of the hypothalamus in female rats
AbstractThe purpose of the study was to determine the functional state of the trophotropic zone of the hypothalamus in female rats of different age groups according to bioelectric activity indices. Experiments were carried out on non-linear white outbred female rats. Animals were divided into IV groups: I group (2.5 months) the juvenile puberty period, II group (eight months) the young age of the reproductive period, III group (fourteen months) the mature age of the reproductive period, IV group (21 months) rats of presenile age of the pronounced senile changes period. Rats of the studied groups underwent Electric Hypothalamus Test (EGtG) of the trophotropic zone. The prevalence of low-frequency components of EGtG in female rats of the juvenile age and the domination of bioelectric activity in the delta range of the investigated hypothalamic region is determined. The young age in females was marked by an increase in all values of normalized spectral power, except the theta-like activity, which in turn was characterized by a possible decrease in the indices. In female rats, from young to presenile age, a gradual increase in the share of absolute power of high-frequency components of EGtG was observed, which was manifested in the functional activation of desynchronizing effect on bioelectric activity of the investigated hypothalamic region. There was an increase in theta-like and beta-like activity while there was a reduction in the percentage of alpha and especially delta wave power. In the female rats of the presenile age, the delta-like activity indices slightly recovered and exceeded those of mature female rats, theta and alpha-like activities underwent a significant decline in values and were represented by the lowest values, while beta-like activity was observed at the highest rates. However, it was precisely the mature female rats that showed a significant predominance of beta-like activity, which is evidence of the powerful desynchronizing mechanisms functioning. Electrophysiological indices indicate synaptic plasticity growth deficiency in female rats of the youngest age and factors of its degradation in those of presenile age, respectively. The power and the desynchronization rhythms representation increase is characteristic of the most productive age period with the most developed neurosynapticplasticity inherent to it.
Amenta, F., Zaccheo, D., & Collier, W. L. (1991). Neurotransmitters, neuroreceptors and aging. Mechanisms of Ageing and Development, 61(3), 249–273.
Arivazhagan, P., & Panneerselvam, C. (2002). Neurochemical changes related to ageing in the rat brain and the effect of DL-alpha-lipoic acid. Experimental Gerontology, 37(12), 1489–1494.
Brockhaus, K., Böhm, R. R., Melkonyan, H., & Thanos, S. (2018). Age-related beta-synuclein alters the p53/Mdm2 pathway and induces the apoptosis of brain microvascular endothelial cells in vitro. Cell Transplantation, 27(5), 796–813.
Chaus, T. G., Ljashenko, V. P., & Tkachenko, J. O. (2015). Zagal’na kharakterystyka elektrychnoji aktyvnostі gіpotalamusu shhurіv za umov stresu ta prygnіchennja kateholergіchnoji nejroperedachі rezerpіnom [General characteristic of electric activity of hypothalamus of rats in the conditions of stress and inhibition of neurotransmission using reserpine]. Prirodnichij Almanah, 41, 167–181 (in Ukrainian).
Cole, D. C., Chung, Y., Gagnidze, K., Hajdarovic, K. H., Rayon-Estrada, V., Harjanto, D., Bigio, B., Gal-Toth, J., Milner, T. A., McEwen, B. S., Papavasiliou, F. N., & Bulloch, K. (2017). Loss of apobeC1 RNA-editing function in microglia exacerbates age-related CNS pathophysiology. Proceedings of the National Academy of Sciences, 114(50), 13272–13277.
Falconi-Sobrinho, L. L., Anjos-Garcia, T. D., de Oliveira, R., & Coimbra, N. C. (2017). Decrease in NMDA receptor-signalling activity in the anterior cingulate cortex diminishes defensive behaviour and unconditioned fear-induced antinociception elicited by GABAergic tonic inhibition impairment in the posterior hypothalamus. European Neuropsychopharmacology, 27(11), 1120–1131.
Felsted, J. A., Chien, C. H., Wang, D., Panessiti, M., Ameroso, D., Greenberg, A., Feng, G., Kong, D., & Rios, M. (2017). Alpha2delta-1 in SF1+ neurons of the ventromedial hypothalamus is an essential regulator of glucose and lipid homeostasis. Cell Reports, 21(10), 2737–2747.
Gomes de Andrade, G., Reck Cechinel, L., Bertoldi, K., Galvão, F., Valdeci Worm, P., & Rodrigues Siqueira, I. (2018). The aging process alters IL-1β and CD63 levels differently in extracellular vesicles obtained from the plasma and cerebrospinal fluid. Neuroimmunomodulation, 25(1), 18–22.
Hernandez, A. R., Reasor, J. E., Truckenbrod, L. M., Campos, K. T., Federico, Q. P., Fertal, K. E., Lubke, K. N., Johnson, S. A., Clark, B. J., Maurer, A. P., & Burke, S. N. (2018). Dissociable effects of advanced age on prefrontal cortical and medial temporal lobe ensemble activity. Neurobiology of Aging, 30(70), 217–232.
Kazakov, V. N., & Natrus, L. V. (2005). Modulation of neuronal impulse activity of the anterior hypothalamus as a functional basis of the mechanisms underlying hypothalamic control. Neurophysiology, 37, 463–474.
Kinawy, A. A., Ezzat, A. R., & Al-Suwaigh, B. R. (2014). Inhalation of air polluted with gasoline vapours alters the levels of amino acid neurotransmitters in the cerebral cortex, hippocampus, and hypothalamus of the rat. Experimental and Toxicologic Pathology, 66(5–6), 219–224.
Lopez, T. P., Giles, K., Dugger, B. N., Oehler, A., Condello, C., Krejciova, Z., Castaneda, J. A., Carlson, G. A., & Prusiner, S. B. (2017). A novel vector for transgenesis in the rat CNS. Acta Neuropathologica Communications, 5(1), 84.
Masliukov, P. M., Emanuilov, A. I., & Nozdrachev, A. D. (2016). Developmental changes of neurotransmitter properties in sympathetic neurons. Advances in Gerontology, 29(3), 442–453.
Musi, N., & Hornsby, P. (2015). Handbook of the biology of aging. 8th edition. Academic Press, New York.
Nabbi-Schroeter, D., Elmenhorst, D., Oskamp, A., Laskowski, S., Bauer, A., & Kroll, T. (2018). Effects of long-term caffeine consumption on the adenosine a1 receptor in the rat brain: An in vivo PET study with [18F] CPFPX. Molecular Imaging and Biology, 20(2), 284–291.
Nakamura, S., Yamao, S., Iijima, S., Kaji, R., Kameyama, M., Miyata, S., Itoh, J., & Mimori, Y. (1984). Age-related changes in nervous system. Nihon Ronen Igakkai Zasshi, 21(3), 209–213.
Paxinos, G., & Watson, C. (2005). The rat brain in stereotaxic coordinates. 5-th edition. Academic Press, New York.
Piechota, M., & Sunderland, P. (2014). Neuronal aging. Postepy Biochemii, 60(2), 177–186.
Ray, S., Corenblum, M. J., Anandhan, A., Reed, A., Ortiz, F. O., Zhang, D. D., Barnes, C. A., & Madhavan, L. (2018). A role for Nrf2 expression in defining the aging of hippocampal neural stem cells. Cell Transplantation, 27(4), 589–606.
Sharma, R. K., Choudhary, R. C., Reddy, M. K., Ray, A., & Ravi, K. (2015). Role of posterior hypothalamus in hypobaric hypoxia induced pulmonary edema. Respiratory Physiology Neurobiology, 205, 66–76.
Straathof, M., Sinke, M. R., van der Toorn, A., Weerheim, P. L., Otte, W. M., & Dijkhuizen, R. M. (2018). Differences in structural and functional networks between young adult and aged rat brains before and after stroke lesion simulations. Neurobiology of Disease, 18, 30393.
Vorobeva, T. M., & Koljadko, S. P. (2007). Jelektricheskaja aktivnost’ mozga (priroda, mehanizmy, funkcionalnoe znachenie) [The electrical activity of the brain (the nature, mechanisms, functional significance)]. Jeksperimentalnaja i Klinicheskaja Medicina, 2, 4–11.
Won, S. H., Jang, H. S., Lee, H. W., Jang, I. S., & Lee, M. G. (2012). Evaluation of brain functional states based on projections of electroencephalographic spectral parameters on 2-dimensional canonical space. Neuroscience Methods, 211(1), 40–48.
Yadegari, M., Khazaei, M., Anvari, M., & Eskandari, M. (2016). Prenatal caffeine exposure impairs pregnancy in rats. International Journal of Fertility Sterility, 9(4), 558–562.
Zapadnjuk, I. P., Zapadnjuk, E. A., & Zaharija, E. A. (1983). Laboratornye zhivotnye: Razvedenie, soderzhanie, ispolzovanie v eksperimente [Laboratory animals: Breeding, maintenance, and use in experiments]. Vishha Shkola, Kyiv (in Ukrainian).
Zaec, N. S., Ljashenko, V. P., Burceva, D. O., Lukashov, S. M., & Melnіkova, O. Z. (2014). Adaptacіjnі reakcіji nejrosinaptichnoji aktyvnostі ergotropnoji zony gіpotalamusa shhurіv za umov luzhnogo racіonu [Adaptational reactions of neurosynaptic activity of ergotropic zone of hypothalamus of rats in the conditions of alkaline ration]. Vchenі Zapiski Tavrіjskogo Nacіonalnogo Unіversitetu іm. V. І. Vernadskogo, Bіologіja, Hіmіja, 27, 46–55 (in Ukrainian).
Zhang, B., Gong, J., Zhang, W., Xiao, R., Liu, J., & Xu, X. Z. S. (2018). Brain-gut communications via distinct neuroendocrine signals bidirectionally regulate longevity in C. elegans. Genes Development, 32(3–4), 258–270.
Zhou, Y. F., Li, P. C., Wu, J. H., Haslam, J. A., Mao, L., Xia, Y. P., He, Q. W., Wang, X. X., Lei, H., Lan, X. L., Miao, Q. R., Yue, Z. Y., Li, Y. N., & Hu, B. (2018). Sema3E/PlexinD1 inhibition is a therapeutic strategy for improving cerebral perfusion and restoring functional loss after stroke in aged rats. Neurobiology Aging, 70, 102–116.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons «Attribution» 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.