Analysis of the transmembrane potential of embryos exposed to action of nickel, cobalt, tin and zinc
AbstractGerm cells of aquatic organisms are complex systems whose growth and development depends on many factors, one of which is the composition of the aquatic environment. We used parameters in our analysis from aggregate data available from published literature. They are data of the transmembrane potential of the germinal cells of Misgurnus fossilis (Linnaeus, 1758) at the development stage from 180th to 360th minutes. Embryos were incubated in an environment with nickel, cobalt, tin, and zinc ions and without them. Plotted lines of the transmembrane potential were digitized and calibrated at intervals of 10 minutes. Rows of numerical values of the transmembrane potentials were obtained. These rows were used for calculation of autocorrelation and cross-cross-correlation functions. It was established that the transmembrane potential describes nonperiodic and quasi-periodic oscillations. The higher statistically significant values of the autocorrelation coefficients were observed in the first lags. Autocorrelation analysis indicates that the periods of oscillations of the transmembrane potential increase with the action of nickel, cobalt, tin and zinc on the germ. The phenomena and processes that occur in the germ cell are well reflected at the initial stages of the auto-correction and are lost when the magnitude of the lag increases. The degree of similarity of transmembrane potentials with the help of cross-correlation analysis is quantitatively characterized. The distribution of fluctuations of cross-correlation functions with complex dynamics, which arise with time shifts both in the forward and reverse directions, were established. It is established that for large values of time shifts, the cross-correlation coefficient is a low-informative indicator, since information about the influence of the factor on the living system is lost. A graph for a given time shift was constructed. The connection between the nodes is the magnitude of the cross-correlation coefficients between the vapor of the transmembrane potentials, which indicate the degree of similarity of the bioelectric processes. Graphs will be used for qualitative and quantitative study of system dynamics. The obtained results confirm the existence of a close relationship between environmental nickel, cobalt, tin, and zinc and the oscillation of transmembrane potential during early embryogenesis.
Behra, R. (1993). In vitro effects of cadmium, zinc and lead on calmodulin-depen dent actions in Oncorhynchus mykiss, Mytilus sp., and Chlamydomonas reinhardtii. Archives of Environmental Contamination and Toxicology, 24(1), 21–27.
Blewett, T. A., Smith, D. S., Wood, C. M., & Glover, C. N. (2016). Mechanisms of nickel toxicity in the highly sensitive embryos of the sea urchin Evechinus chloroticus, and the modifying effects of natural organic matter. Environ mental Science and Technology, 50(3), 1595–1603.
Boiko, N., & Sanagursky, D. (2000). Dynamika transmembrannoho potentsialu za rodkiv v’yuna v umovakh vplyvu ioniv vazhkykh metaliv [Dynamics of trans membrane potential of germ breeding in conditions of influence of heavy metal ions]. Visnyk of Lviv University. Series Biological, 25, 3–7 (in Ukrainian).
Cai, G., Zhu, J., Shen, C., Cui, Y., Du, J., & Chen, X. (2012). The effects of cobalt on the development, oxidative stress, and apoptosis in zebrafish embryos. Biological Trace Element Research, 150(1), 200–207.
Dineley, K. E., Votyakova, T. V., & Reynolds, I. J. (2003). Zinc inhibition of cellular energy production: Implications for mitochondria and neurodegene ration. Journal of Neurochemistry, 85, 563–570.
Fabricio Neta, A. B., do Nascimento, C. W. A., Biondi, C. M., van Straaten, P., & Bittar, S. M. B. (2018). Natural concentrations and reference values for trace elements in soils of a tropical volcanic archipelago. Environmental Geoche mistry and Health, 40(1), 163–173.
Galyk, G. V. (2017). Vplyv ioniv vazhkykh metaliv na metabolizm zarodkovykh klityn [The impact of heavy metals on the metabolism of germ cells]. Experi mental and Clinical Physiology and Biochemistry, 1, 58–65 (in Ukrainian).
Gevorgyan, G. A., Mamyan, A. S., Hambaryan, L. R., Khudaverdyan, S. K., & Vaseashta, A. (2016). Environmental risk assessment of heavy metal pollution in Armenian river ecosystems: Case study of Lake Sevan and Debed River catchment basins. Polish Journal of Environmental Studies, 25(6), 2387–2399.
Goyda, О. А. (1993). Biofizicheskiye aspekty rannego ontogeneza zhivotnykh [Biophysical aspects of early ontogenesis of animals]. Naukova Dumka, Kyiv (in Russian).
Hwang, U. K., Park, J. S., Kwon, J. N., Heo, S., Oshima, Y., & Kang, H. S. (2012). Effect of nickel on embryo development and expression of metallothionein gene in the sea urchin (Hemicentrotus pulcherrimus). Journal Faculty of Agriculture Kyushu University, 57(1), 145–149.
Ivashkiv, L., Hraduk, M., & Sanagursky, D. (2001). Analiz kros-korelyatsiy u chasovykh zminakh fizyko-khimichnykh pokaznykiv rozvytku zarodkiv v'yuna [Analysis of cross-correlations in temporary changes of physical and chemi cal parameters of loach germs' development]. Visnyk of Lviv University. Series Biological, 27, 3–11.
Marzan, L. W., Hossain, M., Mina, S. A., Akter, Y., & Masudul Azad Chowdhury, A. M. (2017). Isolation and biochemical characterization of heavy-metal resis tant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioreme diation viewpoint. The Egyptian Journal of Aquatic Research, 43(1), 65–74.
Sanagursky, D. I., & Goyda, E. A. (1980). Opisaniye biologicheskikh struktur s pozitsiy ikh organizatsii [Description of biological structures from the perspective of their organization]. Bionics Issues, 24, 100–105 (in Russian).
Tellis, M. S., Lauer, M. M., Nadella, S., Bianchini, A., & Wood, C. M. (2014). The effects of copper and nickel on the embryonic life stages of the purple sea urchin (Strongylocentrotus purpuratus). Archives of Environmental Conta mination and Toxicology, 67(3), 453–464.
Tualla, I. P. B., & Bitacura, G. (2016). Effects of cadmium and zinc on the gamete viability, fertilization, and embryonic development of Tripneustes gratilla (Linnaeus). Scientifica (Cairo), 8175213.
Zimmermann, I., Marabelli, A., Bertozzi, C., Sivilotti, L. G., & Dutzler, R. (2012). Inhibition of the prokaryotic pentameric ligand-gated ion channel ELIC by divalent cations. PLoS Biology, 10(11), e1001429.
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