Impact of ions of zinc and cadmium on body weight, fertility and condition of the tissues and organs of Procambarus virginalis (Decapoda, Cambaridae)

  • O. M. Marenkov Oles Honchar Dnipro National University
  • K. K. Holoborodko Oles Honchar Dnipro National University
  • U. S. Voronkova Oles Honchar Dnipro National University
  • O. S. Nesterenko Oles Honchar Dnipro National University
Keywords: Marmorkrebs, crustaceans, heavy metals, hepatopancreas, adipocytes


Heavy metals cause functional disorders in the tissues and organs of hydrobionts, affecting their linear-weight indicators, reproductive system, digestive and eliminative organs. Using the method of histological estimation of the conditions of the tissues and organs of Procambarus virginalis Lyko, 2017 allowed us to determine the peculiarities of adaptation of crustaceans to the effect of different concentrations of heavy metal ions in the conditions of a modeling experiment. We studied the impact ions of zinc (0.1 mg/l) and cadmium (0.01 mg/l) on the organism of P. virginalis. The heavy metal ions had a negative effect on the weight indicators, survivability and reproduction of P. virginalis. During the experiments with zinc, we observed death of 18.2% of the tested сrustaceans; the highest death rate was observed in the experiment with cadmium – 27.3%. Experimentally modeled concentrations of heavy metals negatively affected the reproductive system of P. virginalis. In the experiment with cadmium ions, we observed no spawning; the highest percentage of the females with deposed eggs was observed in the control – 29.1%. The conducted studies allowed us to observe the reaction of the eliminatory system of P. virginalis to the impact of heavy metal ions. The impact of ions of zinc and cadmium decreased the area of secretory cells of the green gland by 10.4–14.8%. We observed grinding of the nuclear cell apparatus. Therefore, the nucleii of granulocytes were 23.6% smaller under the impact of zinc, and 17.5% smaller under the influence of cadmium. The sizes of cells and nucleus apparatus decreased mutually and proportionally; this was proven by stable indicators of nucleus-cytoplasm ratio, its value equaled 0.29–0.31 units. The given concentrations of heavy metals did not affect the histological condition of the adipose tissue, the sizes of adipocytes fluctuated within the range of 215–2178 μm2, the average equaled 872–994 μm2. We determined the negative effect of heavy metals on the histological structure of the hepatopancreas and the value of gland lumens. In the control, the area of previous section equaled 164.5 μm2 with 39 μm2 lumen. During the experiment with zinc ions the structures of hepatopancreas increased by 19.9%, and in the experiment with cadmium, we observed the highest increase in the structural elements of the tissues – by 50.2%. The worst histological condition of the cells of the tested tissues and organs of P. virginalis was observed among individuals impacted by the cadmium ions; this is explained by the high toxic effect of this heavy metal. 


Alwes, F., & Scholtz, G. (2006). Stages and other aspects of the embryology of the parthenogenetic Marmorkrebs (Decapoda, Reptantia, Astacida). Development Genes and Evolution, 216(4), 169–184.

Benli, A. C. K. (2015). The influence of etofenprox on narrow clawed crayfish (Astacus leptodactylus Eschscholtz, 1823): Acute toxicity and sublethal effects on histology, hemolymph parameters, and total hemocyte counts. Environmental Toxicology, 30(8), 887–894.

Bohman, P., Edsman, L., Martin, P., & Scholtz, G. (2013). The first Marmorkrebs (Decapoda: Astacida: Cambaridae) in Scandinavia. BioInvasions Records, 2(3), 227–232.

Chucholl, C., & Pfeiffer, M. (2010). First evidence for an established Marmorkrebs (Decapoda, Astacida, Cambaridae) population in Southwestern Germany, in syntopic occurrence with Orconectes limosus (Rafinesque, 1817). Aquatic Invasions, 5(4), 405–412.

Faulkes, Z. (2010). The spread of the parthenogenetic marbled crayfish, Marmorkrebs (Procambarus sp.), in the North American pet trade. Aquatic Invasions, 5(4), 447–450.

Faulkes, Z. (2015). Marmorkrebs (Procambarus fallax f. virginalis) are the most popular crayfish in the North American pet trade. Knowledge and Management of Aquatic Ecosystems, 416, 20–35.

Fedonenko, O. V., Esipova, N. B., Sharamok, T. S., Ananieva, T. V., Yakovenko, V. A., & Zhezhera, V. A. (2012). Suchasni problemy hidroekolohiyi: Zaporizke vodoskhovyshche [Modern problems in hydroecology: Zaporizhzhya Reservoir], Lira, Dnipropetrovsk (in Ukrainian).

Fedonenko, O. V., Sharamok, T. S., & Yesipova, N. B. (2010). Raspredelenie svinca i kadmiya v ehkosisteme samarskogo rybovodnogo pruda [Distribution of lead and cadmium in the ecosystem of the Samara fish pond]. Visnyk of Kharkiv National University, 6, 210–216 (in Russian).

Hobbs, H. H. (1942). The crayfishes of Florida. Biological Science Series, 3(2), 1–179.

Holoborodko, K. K., Marenkov, O. M., Gorban ,V. A., & Voronkova, Y. S. (2016). The problem of assessing the viability of invasive species in the conditions of the steppe zone of Ukraine. Visnyk of Dnipropetrovsk University. Biology, Ecology, 24(2), 466–472.

Jimenez, A. S., & Faulkes, Z. (2010). Establishment and care of a laboratory colony of parthenogenetic marbled crayfish, Marmorkrebs. Invertebrate Rearing, 1, 10–18.

Kawai, T., & Takahata, M. (2010). The biology of freshwater crayfish. Hokkaido University Press, Sapporo.

Lipták, B., Mrugała, A., Pekárik, L., Mutkovič, A., Gruľa, D., Petrusek, A., & Kouba, A. (2016). Expansion of the marbled crayfish in Slovakia: Beginning of an invasion in the Danube catchment? Journal of Limnology, 75(2), 305–312.

Martin, P., Dorn, N. J., Kawai, T., van der Heiden, C., & Scholtz, G. (2010a). The enigmatic Marmorkrebs (marbled crayfish) is the parthenogenetic form of Procambarus fallax (Hagen, 1870). Contributions to Zoology, 79, 107–118.

Martin, P., Shen, H., Füllner, G., & Scholtz, G. (2010b). The first record of the parthenogenetic Marmorkrebs (Decapoda, Astacida, Cambaridae) in the wild in Saxony (Germany) raises the question of its actual threat to European freshwater ecosystems. Aquatic Invasions, 5, 397–403.

Martin, P., Thonagel, S., & Scholtz, G. (2016). The parthenogenetic Marmorkrebs (Malacostraca: Decapoda: Cambaridae) is a triploid organism. Journal of Zoological Systematics and Evolutionary Research, 54(1), 13–21.

Marzano, F. N., Scalici, M., Chiesa, S., Gherardi, F., Piccinini, A., & Gibertini, G. (2009). The first record of the marbled crayfish adds further threats to fresh waters in Italy. Aquatic Invasions, 4(2), 401–404.

Mirenda, R. J. (1986). Toxicity and accumulation of cadmium in the crayfish, Orconectes virilis (Hagen). Archives of Environmental Contamination and Toxicology, 15(4), 401–407.

Mumford, S., Heidel, J., Smith, C., Morrison, J., Macconnell, B., & Blazer, V. (2007). Fish histology and histopathology. US Fish and Wildlife Service, Washington.

Novitsky, R. A., & Son, M. O. (2016). The first records of Marmorkrebs [Procambarus fallax (Hagen, 1870) f. virginalis] (Crustacea, Decapoda, Cambaridae) in Ukraine. Ecologia Montenegrina, 5, 44–46.

Opp, C., Hahn, J., Zitzer, N., & Laufenberg, G. (2015). Heavy metal concentrations in pores and surface waters during the emptying of a small reservoir. Journal of Geoscience and Environment Protection, 3, 66–72.

Patoka, J., Kalous, L., & Kopecký, O. (2015). Imports of ornamental crayfish: The first decade from the Czech Republic’s perspective. Knowledge and Management of Aquatic Ecosystems, 416, 4–13.

Peay, S., Holdich, D. M., & Brickland, J. (2010). Risk assessments of non-indigenous crayfish in Great Britain. Freshwater Crayfish, 17, 109–122.

Scholtz, G., Braband, A., Tolley, L., Reimann, A., Mittmann, B., Lukhaup, C., Steuerwald, F., & Vogt, G. (2003). Parthenogenesis in an outsider crayfish. Nature, 421(6925), 769–873.

Shields, J. D., & Boyd, R. (2014). Atlas of lobster anatomy and histology. Virginia Institute of Marine Science, Gloucester Point.

Taylor, C. A., Warren, M. L., Fitzpatrick, J. F., Hobbs, H. H., Jezerinac, R. F., Pflieger, W. L., & Robison, H. W. (1996). Conservation status of crayfishes of the United States and Canada. Fisheries, 21(4), 25–38.

Vogt, G. (2010). Suitability of the clonal marbled crayfish for biogerontological research: A review and perspective, with remarks on some further crustaceans. Biogerontology, 11(6), 643–669.

Vogt, G., Falckenhayn, C., Schrimpf, A., Schmid, K., Hanna, K., Panteleit, J., Helm, M., Schulz, R., & Lyko, F. (2015). The marbled crayfish as a paradigm for saltational speciation by autopolyploidy and parthenogenesis in animals. Biology Open, 4(11), 1583–1594.

Wigginton, A. J., & Birge, W. J. (2007). Toxicity of cadmium to six species in two genera of crayfish and the effect of cadmium on molting success. Environmental Toxicology and Chemistry, 26(3), 548–554.

How to Cite
Marenkov, O. M., Holoborodko, K. K., Voronkova, U. S., & Nesterenko, O. S. (2017). Impact of ions of zinc and cadmium on body weight, fertility and condition of the tissues and organs of Procambarus virginalis (Decapoda, Cambaridae). Regulatory Mechanisms in Biosystems, 8(4), 628–632.