Impact of new triazole-based growth regulators on winter ontogenesis of new winter wheat varieties
Abstract
Triazole-derived compounds are increasingly regarded as promising tools for improving the adaptability and productivity of winter wheat under unstable environmental conditions. Their physiological activity is associated with regulation of growth pr o cesses, enhancement of stress tolerance , and support of metabolic functions linked with overwintering. In t he present study , we evaluated the effects of three new triazole -type compound s, CA-64, CA-79 and CA-67, on winter period performance of five winter wheat varieties Khvylia Dnipra, MIP Roksolana, Vezha, LG Magirus , and LG Litopys. Seeds (1,000 per treatment) were soaked for 24 h in aqueous solutions of the tested compounds at concentrations of 0.01%, 0.02%, and 0.04%; water-treated seeds served as the control. The analysis focused on seed germination, plant survival before and after winter , and soluble sugar content in the tillering node on selected winter dates . The results demonstrated a clear concentration-dependent response. Among the tested agents, CA-79 showed the strongest and most stable stimulating effect, especially at 0.02%, which provided the highest values of germination, survival of plants during winter , and carbohydrate accumulation in the tillering node across most gen o types. CA-64 also produced a positive effect, particularly at 0.02%, while 0.01% was beneficial in several cases and may be regarded as a safer conservative option. By contrast, CA-67 did not provide reliable stimulation, 0.01% was mostly neutral, whereas 0.02% and especially 0.04% reduced sugar reserv es and worsened the survival-related traits. All three compounds were uniformly depressive at a concentration of 0.04%, causing declines in germination, pre-winter plant survival , post-winter survival, and winter carbohydrate status, thus indicating a toxicity threshold. The response was genotype-specific. The U krainian varieties Khvylia Dnipra, MIP Roksolana , and Vezha generally exhibited higher baseline winter-resistance parameters and responded more positively to CA-79 and CA-64, whereas the F rench varieties LG Magirus and LG Litopys had lower baseline sugar levels and greater sensitivity to excessive concentration. In practical terms, CA-79 at 0.02% should be considered the most promising option for enhancing winter tolerance, while CA-64 at 0.02% may serve as an effective alternative. CA-67 cannot be reco m mended as a reliable stimulator under the studied conditions. Further studies should integrate these results with grain yield and grain quality traits to develop variety-specific protocols for the use of novel triazole-based growth regulators in winter wheat.References
Ali, S., & Baloch, A. M. (2020). Overview of sustainable plant growth and differentiation and the role of hormones in controlling growth and development of plants under various stresses. Recent Patents on Food, Nutrition and Agriculture, 11(2), 105–114.
Brygadyrenko, V. V., & Reshetniak, D. Y. (2014). Trophic preferences of Harpalus rufipes (Coleoptera, Carabidae) with regard to seeds of agricultural crops in conditions of laboratory experiment. Baltic Journal of Coleopterology, 14(2), 179–190.
Cohen, I., Zandalinas, S. I., Huck, C., Fritschi, F. B., & Mittler, R. (2021). Meta-analysis of drought and heat stress combination impact on crop yield and yield components. Physiologia Plantarum, 171(1), 66–76.
Du, X., Du, Y., Feng, N., Zheng, D., Zhou, H., & Huo, J. (2024). Exogenous Uniconazole promotes physiological metabolism and grain yield of rice under salt stress. Frontiers in Plant Science, 15, 1459121.
Ejaz, I., Pu, X., Naseer, M. A., Bohoussou, Y. N. D., Liu, Y., Farooq, M., Zhang, J., Zhang, Y., Wang, Z., & Sun, Z. (2023). Cold and drought stresses in wheat: A global meta-analysis of 21st century. Journal of Plant Growth Regulation, 42(9), 5379–5395.
Gao, C., Zhao, H., Zhao, F., Kong, W., Ju, F., Li, Z., Shi, D., & Liu, P. (2025). Effect of growth regulators on the stem characteristics and yield of summer maize in maize-soybean strip intercropping. Scientia Agricultura Sinica, 58(23), 4920–4935.
Hu, F., Lu, J., Zhai, L., Qiu, X., Du, B., & Xu, J. (2025). Effects of foliar application of Paclobutrazol on grain yield, aroma, and canopy radiation use efficiency of aromatic rice. Biology, 14(11), 1562.
Janda, T., Szalai, G., & Pál, M. (2020). Salicylic acid signaling in plants. International Journal of Molecular Sciences, 21(7), 2655.
Jha, U. C., Bohra, A., & Jha, R. (2017). Breeding approaches and genomics technologies to increase crop yield under low-temperature stress. Plant Cell Reports, 36, 1–35.
Korsukova, A. V., Lyubushkina, I. V., Zabanova, N. S., Berezhnaya, E. V., Polyakova, E. A., Pobezhimova, T. P., Kirichenko, K. A., Dorofeev, N. V., Dudareva, L. V., & Grabelnych, O. I. (2025). Mechanisms of increase of winter wheat frost resistance under Tebuconazole treatment at early stage of growth: Role of hormone- and reactive oxygen species-mediated signaling pathways. Plants, 14(3), 314.
Kosová, K., Klíma, M., Prášil, I. T., & Vítámvás, P. (2021). COR/LEA proteins as indicators of frost tolerance in triticeae: A comparison of controlled versus field conditions. Plants, 10(4), 789.
Lamlom, S. F., Abdelghany, A. M., Farouk, A. S., Alwakel, E. S., Makled, K. M., Bukhari, N. A., Hatamleh, A. A., Ren, H., El-Sorady, G. A., & Shehab, A. A. (2025). Biochemical and yield response of spring wheat to drought stress through gibberellic and abscisic acids. BMC Plant Biology, 25, 5.
Li, H., Li, T., Weng, W., Cui, G., Zhang, H., Xing, Z., Fu, L., Liu, B., Wei, H., Zhang, H., & Li, G. (2025). Chlormequat chloride and Uniconazole regulate lodging resistance and yield formation of wheat through different strategies. Agronomy, 15(11), 2475.
Mohsin, S. M., Hasanuzzaman, M., Parvin, K., Shahadat, H., & Parvin, K. (2021). Protective role of Tebuconazole and Trifloxystrobin in wheat (Triticum aestivum L.) under cadmium stress via enhancement of antioxidant defense and glyoxalase systems. Physiology and Molecular Biology of Plants, 27, 1043–1057.
Nazarenko, M., & Khoroshun, I. (2025). Estimation of effectivity of new growth regulators on winter wheat productivity and quality. Regulatory Mechanisms in Biosystems, 16(4), e25190.
Nazarenko, M., Izhboldin, O., & Izhboldina, O. (2022). Study of variability of winter wheat varieties and lines in terms of winter hardness and drought resistance. AgroLife Scientific Journal, 11(2), 116–123.
Nazarenko, M., Okselenko, O., & Pozniak, V. (2023). Ecology- and geography-related features of winter wheat varieties for the areas of insufficient humidification. Agriculture and Forestry, 69(3), 159–177.
Parizi, S. S., Ilkaee, M. N., Paknejad, F., AghaYari, F., & Sadeghi Shoa, M. (2025). Efficiency of paclobutrazol and gibberellic acid in wheat (Triticum aestivum L.) under different doses of nitrogen fertilization. Journal of Plant Nutrition, 48(4), 690–702.
Rani, S., Chaudhary, A., & Rani, K. (2018). Management strategies forabiotic stresses in barley. Wheat and Barley Research, 10(3), 151–165.
Rowshani, R., Soleymani, A., Mahlooji, M., & Naderi, M. R. (2024). Plant growth regulators affecting the physiological and yield responses of field barley genotypes to the drought and salinity stresses. Theoretical and Applied Climatology, 155, 9517–9528.
Shaki, F., Rezayian, M., Ebrahimzadeh, H., & Niknam, V. (2022). Role of triazolic compounds in underlyingmechanisms of plant stress tolerance: A review. Iranian Journal of Plant Physiology, 12(1), 3943–3954.
Wang, Y., Zhao, L.-M., Feng, N., Zheng, D., Shen, X. F., Zhou, H., Jiang, W., Du, Y., Zhao, H., Lu, X., & Deng, P. (2024). Plant growth regulators mitigate oxidative damage to rice seedling roots by NaCl stress. PeerJ, 12, e17068.
Xu, T., Wang, D., Si, Y., Kong, Y., Shao, X., Geng, Y., Lv, Y., & Wang, Y. (2024). Plant growth regulators enhance maize (Zea mays L.) yield under high density by optimizing canopy structure and delaying leaf senescence. Agronomy, 14(6), 1262.
Zhang, W., Duan, X., Li, M., Du, B., Liang, Z., Wang, Y., Tang, Y., Li, J., & Yao, X. (2025). Foliar uniconazole application increases rice lodging resistance by altering stem morphological and anatomical traits. Crop and Environment, 4(1), 1–13.
Zhang, Y., He, Z., Xing, P., Luo, H., Yan, Z., & Tang, X. (2024). Effects of paclobutrazol seed priming on seedling quality, photosynthesis, and physiological characteristics of fragrant rice. BMC Plant Biology, 24, 53.
Zhao, Q., Xiong, R., Tang, J., Yang, R., Cao, L., & Bai, J. (2025). Effects of different spraying periods and concentrations of Paclobutrazol on the lodging resistance and yield of high-quality japonica rice. Frontiers in Agronomy, 7, 1603904.
Zhong, X., Wu, Q., Yang, L., Lin, Z., Ren, D., Li, Z., Zhou, Y., & Duan, L. (2026). Brazide: A breakthrough plant growth regulator that delays leaf senescence under heat stress to promote grain yield in winter wheat (Triticum aestivum L.). Field Crops Research, 337, 110265.
Zhou, H., Liu, L., Zhou, J., He, A., & Wu, Z. (2025). Biological agents and plant growth regulator promote rice growth and regenerative capacity. BMC Plant Biology, 25, 1540.
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