Detection of Escherichia coli with acquired resistance to beta-lactams and carbapenems in animal and poultry feed in Ukraine

N. Kuriata; Y. Saliga; I. Rublenko; O. Gorbatiuk; O. Pishchanskyi; I. Musiіets; V. Ukhovskyi; M. Ruda; O. Chechet

doi:10.15421/0226007

N. Kuriata Institute of Animal Biology of the National Academy of Agrarian Sciences of Ukraine
Y. Saliga Institute of Animal Biology of the National Academy of Agrarian Sciences of Ukraine
I. Rublenko Bila Tserkva National Agrarian University
O. Gorbatiuk State Research Institute for Laboratory Diagnostics and Veterinary-Sanitary Expertise
O. Pishchanskyi State Research Institute for Laboratory Diagnostics and Veterinary-Sanitary Expertise
I. Musiіets State Research Institute for Laboratory Diagnostics and Veterinary-Sanitary Expertise
V. Ukhovskyi State Research Institute for Laboratory Diagnostics and Veterinary-Sanitary Expertise
M. Ruda State Research Institute for Laboratory Diagnostics and Veterinary-Sanitary Expertise
O. Chechet Odesa State Agrarian University

Keywords: mineral feed, organic feed, artificial feed, compound feed, meal, oilcake, feed grain, premixes, E. coli, acquired resistance, beta-lactamases, ESBL enzymes, AmpC enzymes, carbapenemases, OXA-48 and OXA-48-like enzymes.

Abstract

The materials of the article are devoted to one of the directions for maintaining the biological safety of raw materials and livestock products through ensuring the safety of animal and poultry feed in accordance with the provisions of the global “One Health” concept. Escherichia coli strains isolated from samples of animal and poultry feed, when tested for susceptibility to cep h alosporin antibiotics, revealed one of the hazardous factors – the production of acquired resistance enzymes, namely beta- lactamases and carbapenemases . During microbiological examination of 382 samples of mineral, organic, and artificial feed for animals and poultry, 21 (5.5% of the tested samples) E. coli cultures were isolated and identified. In 13 cases (61.9% of the is o lates), the highest level of contamination with E scherichia was detected in compound feed, bran, meal, and oilcake samples. In 6 cases (28.6%), contamination was detected in concentrated feed (feed grain), and in 2 cases (9.5%) in premixes. The article pr e sents the results of screening 21 experimental E. coli strains to identify those likely to produc e extended-spectrum beta- lactamases (ESBL) and class C beta- lactamases ( AmpC enzymes) using the disk diffusion method with indicator cephalosporins – cefotaxime (5 μg ) and ceftazidime (10 μg ). Experimental strains with inhibition zone diameters below the EUCAST established breakpoints (<20 and <22 mm, respectively) were selected. Such strains raised suspicion of possible production of acquired r e sistance enzymes. The production of extended-spectrum beta- lactamases (ESBL) was confirmed in five E. coli experimental strains (Ec18, Ec31, Ec53, Ec57, Ec64) using double-disk synergy and combination disk diffusion methods. Analysis of exper i mental results also confirmed the production of AmpC beta- lactamases in three E. coli strains (Ec31, Ec53, Ec69 ). These data were verified using the disk diffusion method with the indicator cephalosporin cefoxitin (30 μg ), based on comparison with the EUCAST breakpoint of <19 mm for this antibiotic. The article also presents data on the detection of carbapenemase production using the primary disk diffusion method with meropenem (10 μg ), an alternative method with ertapenem (10 μg ), and an add i tional method using meropenem (20 μg ) and temocillin (30 μg ). According to the analysis of test results, no carbapenemase -producing E. coli strains (including OXA-48 and OXA-48-like enzymes) were identified among the tested isolates. The obtained data provide experimental justification for including microbiological testing of feed industry products in Ukraine in the State Monitoring Program for determining antimicrobial susceptibility of escherichial isolates contaminating animal and poultry feed. This would enable detection and confirmation of beta- lactamase and carbapenemase production in accordance with the National Action Plan to Combat Antimicrobial Resistance and contribute to reducing the risks of dissemination of such microorganisms in animal husbandry for the protection of human and animal health.

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Detection of Escherichia coli with acquired resistance to beta-lactams and carbapenems in animal and poultry feed in Ukraine

Abstract

References

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