Neutrophils stimulation index in people under consumption of broiler chickens meat at pre-slaughter stress correction
AbstractThe data about changes in neutrophils stimulation index in men blood after consumption of broiler chicken meat with the natural origin immunomodulators, introduced in feed before slaughter, is presented in this paper. Spleen extract biologically active substances were used as immunomodulators and anti-stressors during pre-slaughter period. Biologically active substances influence on putrescin, spermine and spermidine content in broiler chicken blood before slaughter and on some non-specific resistance indices in people was determined after consumption of broiler chicken meat. Two groups of broiler chickens at one month age were formed for the study. The spleen extract obtained with ultrasound application (I research group) served as biologically active substances was added to the feed of broiler chickens in pre-slaughter period (five days before slaughter). Blood polyamines such as putrescin, spermine and spermidine were determined by the method of High-performance liquid chromatography (HPLC) on the liquid chromatograph Agilent 1200 (USA). The second experiment was conducted on 10 people. We recruited 10 healthy male medical students (20 years old, on average) after the National Medical license examination. Spleen extract polyamines as immunomodulators and anti-stressors have the most effective influence on total quantity of polyamines in broiler chicken blood. As a result of research, it is found that aerosol introduction of spleen extract into broiler chicken feed reliably increases total quantity of polyamines by 39% and, in particular, spermidine concentration by 34%, and spermine by 40% compared with broiler chickens of the control group. Some non-specific body resistance indices in men blood upon consumption of broiler chicken meat varied within the physiological norm. The neutrophils stimulation index increased in men blood (+0,82) after consumption of meat of broiler chickens to which spleen extract as immunomodulator and anti-stressor was introduced before slaughter (experimental group). The neutrophils stimulation index decreased in men blood (–2,21) after consumption of broiler chickens meat at pre-slaughter stress (control group). The neutrophils stimulation index in men blood of experimental and control groups differed by 34,8%, but did not go beyond the physiological norm. At the final stage of poultry feeding it is necessary to consider pre-slaughter stress and to apply biologically active substances of natural origin, such as spleen extract. The results obtained in the experiment on broiler chickens can be used in studies of non-specific resistance indices of the farm animals for increasing the organism resistance, correction and avoiding of pre-slaughter stress and improvement of product quality
Bespalov, V.G., 2008. Pitanie i rak. Dieticheskaja profilaktika onkologicheskih zabolevanij [Nutrition and cancer. Dietary prevention of cancer]. JuSMA, Moscow (іn Russian).
Brodal, B.P., Eliassen, K.A., Ronning, H., Osmundsen, H., 1999. Effects of dietary polyamines and clofibrate on metabolism of polyamines in the rat. J. Nutr. Biochem. 10, 700–708. >> doi: 10.1016/S0955-2863(99)00058-3
Catros-Quemener, V., Chamaillard, L., Bouet, F.M., 1999. Les polyamines: Role diagnostique et cible therapeutique en cancerologie. S-Medecine / Sciences 15, 1078–1085 (in French). >> doi: 10.4267/10608/1221
De Benedette, M., De Olson, J.W., Snow, E.C., 1993. Expression of polyamine transporter activity during B-lymphocyte cell cycle progression. The Journal of Immunology 150(10), 4218–4224.
Doll, R., Pito, R., 1984. Prichiny raka: Kolichestvennaja ocenka ustranimyh faktorov riska onkologicheskih zabolevanij v SShA [Causes of cancer: Quantitative estimation of avoidable risks of cancer in the USA]. Naukova Dumka, Kiev (іn Russian).
Frimel, G., 1987. Imunololgicheskie metody [Immunological methods]. Medicine, Moscow (іn Russian).
Galitsopoulou, A., Michaelidou, A.M., Menexes, G., Alichanidis, E., 2015. Polyamine profile in ovine and caprine colostrum and milk. Food Chem. 173, 80–85. >> doi: 10.1016/j.foodchem.2014.09.168
Gerbaut, L., 1991. Determination of erythrocytic polyamines by reversed-phase liquid chromatography. Clin. Chem. 37(12), 2117–2120.
Grabovskyi, S.S., 2013. Jekstragirovanie biologicheski aktivnyh veshhestv selezenki s ispolzovaniem ultrazvuka [Extracting of biologically active substances of spleen with the application an ultrasound]. Sbornik Nauchnyh Trudov SWorld. 49, 3–6 (in Russian).
Grabovskyi, S.S., 2013. Vlijanie biologicheski aktivnyh veshhestv raznogo proishozhdenija na laboratornyh zhivotnyh v stressovom sostojanii [Different origin biologically active substances influence on laboratory animals at stress state]. Sbornik Nauchnyh Trudov SWorld 44, 13–15 (іn Russian).
Grabovskyi, S.S., 2014. Vplyv imunomodulyatoriv pryrodnoho pokhodzhennya na pokaznyky klitynnoho imunitetu i riven kortyzolu v krovi shchuriv za umov stresu [Effect of natural immunomodulators influence on cellular immunity indices and cortisol level in rat’s blood at pre-slaughter stress]. Biolohichni Studiyi – Studia Biologica 8(1), 93–102 (іn Ukrainian).
Grabovskyi, S.S., 2014. Vplyv imunomodulyatoriv pryrodnoho pokhodzhennya na vmist insulinu u krovi kurchat-broyleriv za umov stresu [Natural immunomodulators influence on insulin content in broiler chickens blood at pre-slaughter stress]. ScienceRise 1(1), 67–69 (іn Ukrainian).
Hashemi, S.M., Loh, T.C., Foo, H.L., Zulkifli, I., Hair-Bejo, M., 2014. Dietary putrescine effects on performance parameters, nutrient digestibility, intestinal morphology and tissue polyamine content of broilers fed low protein diet. Iran. J. Vet. Res. 15(4), 385–391.
Kalač, P., 2014. Health effects and occurrence of dietary polyamines: A review for the period 2005 – mid 2013. Food Chemistry 161, 27–39.
Kalac, P., Krausová, P., 2005. A review of dietary polyamines: Formation, implications for growth and health and occurrence in foods. Food Chemistry 90, 219–230. >> doi: 10.1016/j.foodchem.2004.03.044
Lapovets, L.Y., Lutsyk, B.D., 2002. Posibnyk z laboratornoyi imunolohiyi [Handbook of laboratory immunology]. Lviv (іn Ukrainian).
Maslyanko, R.P., Grabovskyi, S.S., Grabovska, O.S., 2013. Suchasni uyavlennya pro fahotsytoz [Modern notion of phagocytosis]. Biolohiya Tvaryn 15(3), 63–69 (іn Ukrainian).
Minois, N., Carmona-Gutierrez, D., Madeo, F., 2011. Polyamines in aging and disease. Aging (Albany NY) 3(8), 716–732. >> doi: 10.18632/aging.100361
Nitta, T., Igarashi, K., Yamashita, A., Yamamoto, M., Yamamoto, N., 2001. Involvement of polyamines in B cell receptor-mediated apoptosis: Spermine functions as a negative modulator. Exp. Cell Res. 265(1), 174–183. >> doi: 10.1006/excr.2001.5177
Pohlenz, C., Buentello, A.J., Helland, S., Gatlin, D.M., 2014. Effects of dietary arginine supplementation on growth, protein optimization and innate immune response of channel catfish Ictalurus punctatus (Rafinesque 1818). Aquacult. Res. 45(3), 491–500.
Reyes-Becerril, M., Ascencio-Valle, F., Tovar-Ramírez, D., Meseguer, J., Esteban, M.A., 2011. Effects of polyamines on cellular innate immune response and the expression of immune-relevant genes in gilthead seabream leucocytes. Fish Shellfish Immunol. 30, 248–254. >> doi: 10.1016/j.fsi.2010.10.011
Soda, K., Kano, Y., Sakuragi, M., Takao, K., Lefor, A., Konishi, F., 2009. Long-term oral polyamine intake increases blood polyamine concentrations. J. Nutr. Sci. Vitaminol. 55, 361–366. >> doi: 10.3177/jnsv.55.361
Vargas, A.J., Ashbeck, E.L., Thomson, C.A., Gerner, E.W., Thompson, P., 2014. A dietary polyamine intake and polyamines measured in urine. Nutr. Cancer 66(7), 1144–1153. >> doi: 10.1080/01635581.2014.949801
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons «Attribution» 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.