The patterns of utilization of sulfate and nitrate ions by bacteria Desulfomicrobium sp. CrR3 and Desulfovibrio desulfuricans Ya-11

  • L. S. Dorosh Ivan Franko National University of Lviv
  • T. B. Peretyatko Ivan Franko National University of Lviv
  • S. P. Gudz Ivan Franko National University of Lviv
Keywords: sulfate-reducing bacteria, sulfate reduction, nitrate reduction


The aim of this work was to study the patterns of utilization of sulfate and nitrate ions by bacteria Desulfomicrobium sp. CrR3 and Desulfovibrio desulfuricans Ya-11 under different cultivation conditions. Chromium-resistant sulfate-reducing bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 were used. Bacteria were grown in Posgate C medium at 30°C in 25 ml test tubes under anaerobic conditions. To test the ability of bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 to use various substances and ions as electron acceptors, they were incubated in potassium phosphate buffer (10 mM, pH 7) with sulfate, nitrate and nitrite ions in concentrations of 1, 5 and 10 mM. At various concentrations of sulfate ions (1, 5 and 10 mM), biomass of bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 increased with the increase of concentration of electron acceptor, the maximum biomass was equal to 3.65 and 3.05 g/l at 10 mM of sulfate ions, respectively. With the increase of concentration of nitrate ions to 5 mM the biomass increased by 70% compared to the biomass of bacteria grown in the medium with nitrate ions at the concentration 1 mM. The maximal biomass was determined in the presence of nitrate ions at a concentration of 10 mM – 3.78 and 3.15 g/l for bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11, respectively. It is found, as a result of incubation of bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11, that by introducing sulfate ions at a concentration of 5 mM bacteria Desulfomicrobium sp. CrR3 used 98%, while D. desulfuricans Ya-11 used only 86%, and under these conditions hydrogen sulfide has been detected in the incubation mixture at the concentration of 0.8–1.0 mM. In the presence of 10 mM of sulfate ions efficiency of electron acceptors utilization was equal to 85–95% for both strains. Bacteria Desulfomicrobium sp. CrR3 intensively used nitrate ions, the efficiency of electron acceptor utilization at 10 mM was equal to 92.8%, while for D. desulfuricans Ya-11 the usage percent amounted to 73% only, and nitrite ions were not observed after three days of incubation. It is established that bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 are capable to use sulfate and nitrate ions as electron acceptors in the process of the disimilatory sulfate and nitratre reduction. As a result of the study of patterns of nitrate utilization by Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 it is found that bacteria use nitrate as a nitrogen source for biosynthetic processes, and as electron acceptors. Under these conditions nitrates are reduced to nitrites, and then they are turned to ammonium.


Bailey, N.T.J., 1995. Statistical methods in biology. 3rd edition. Cambridge Univ. Press, Cambridge.

Bratcova, S., Groudev, S., Georgiev, P., 2002. The effect of some essential environmental factors on the microbial dissimilatory sulphate reduction. Mining and Mineral Processing 44(2), 123–127.

Dzhigireji, V.S., 2004. Ekologija ta ohorona navkolyshn’ogo seredovyshha [Ecology and environment]. Znannya, Kyiv (in Russian).

Granger, D.L., Taintor, R.R., Boockvar, K.S., 1996. Measurement of nitrate and nitrite in biological samples using nitrate reductase and Griess reaction. Method. Enzymol. 268, 142–151. >> doi: 10.1016/S0076-6879(96)68016-1

Isakova, O.P, Tarasevych, Y., Yuzjuk, Y., 2009. Processing and visualization of data with the physical experiments by usage Origin package. Libkom, Moscow.

Ivančić, I., Degobbis, D., 1984. An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method. Wat. Res. 18, 1143–1147. >> doi: 10.1016/0043-1354(84)90230-6

Kosinska, K., Miskiewicz, T., 1999. Upgrading the efficiency of dissimalotory sulphate reduction by Desulfovibrio desulfuricans via adjustment of the C0D/S04 ratio. Biotechnol. Lett. 21, 299–302.

Marietou, A., Richardson, D.J., Cole, J.S., 2005. Nitrate reduction by Desulfovibrio desulfuricans: A periplasmic nitrate reductase system that lacks NapB, but includes a unique tetraheme c-type cytochrome, NapM. FEMS Microbiol. Lett. 248, 217–225.

Marietou, А., Griffits, L., 2008. Preferential reduction of the thermodynamically less favorable electron acceptor, sulfate, by nitrate-reducing strain of the sulfate-reducing bacterium Desulfovibrio desulfuricans 27774. J. Biol. 191(3), 882–889.

Mohanakrishnan, J., Kofoed, M.V., Barr, J., Yuan, Z., Schramm, A., Meyer, R.L., 2011. Dynamic microbial response of sulfidogenic wastewater biofilm to nitrate. Appl. Microbiol. Biotechnol. 91(6), 164–757. >> doi: 10.1007/s00253-011-3330-3

Okabe, S., Ito, T., Satoh, H., 2003. Sulphate-reducing bacterial community structure and their contribution to carbon mineralization in a wastewater biofilm growing under microaerophilic conditions. Appl. Microbiol. Biotechnol. 63, 322–334.

Peretjatko, T.B., Galushka, A.A., Gudz', S.P., 2009. Vykorystannja metaliv jak kincevyh akceptoriv elektroniv sul'fatvidnovljuval'nymy bakterijamy [The use of metals as final acceptor of electrons sulphate-reducing bacteria]. Biologichni Studii 3(3), 141–158 (in Ukrainian).

Peretyatko, T.B., Galushka, A.A., Hnatush, S.O., 2006. Vykorystannya orhanichnyh spoluk sul’fatvidnovlyu-val’nymy bakteriyamy rodu Desulfovibrio [Using organic compounds sulfate-reducing bacteria of the genus Desulfovibrio]. Naukovyj visnyk Uzhhorods’koho Universytetu 18, 157–160 (in Ukrainian).

Plugge, C.M., Weiwen, Z., Johannes, C.M., Alfons, J.M., 2011. Metabolic flexibility of sulfate-reducing bacteria. Front. Microbiol. 81(2), 1–8. >> doi: 10.3389/fmicb.2011.00081

Pochvy. Metod opredelenie ionov sulfata v vodnoji vyiityazhke [Soil. Method for determination of sulfate ions in water extract]. GOST 26426-85. Izdatelstvo Standartov, Moskow (in Russian).

Polanco, F.F, Polanco, M.F., Uruena, M.A., Garcia, P.A., Villaverde, S., 2001. Combining the biological nitrogen and sulphur cycles in anerobic conditions. Wat. Sci. Tech. 44(8), 77–84.

Postgate, J.R., 1984. The sulfate-reducing bacteria. 2nd ed. Cambridge Univ. Press, Cambridge.

Sholyak, K.V., Peretyatko, T.B., Gudz’, S.P., 2013. Sul’fatvidnovlyuval’ni bakteriyi, stijki do pidvyshhenyx koncentracij shestyvalentnoho xromu [Sulfate-reducing bacteria resistant to high concentration of hexavalent chromium]. Mikrobiolohiya i Biotechnolohiya 2, 66–76 (in Ukrainian).

Sugiyama, M., 2002. Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen sulfide. United States Patent. № 6340596.

Szewzyk, R., Pfennig, N., 1987. Complete oxidation of catechol by the strictly anaerobic sulfate-reducing Desulfobacterium catecholicum sp. nov. Arch. Microbiol. 147, 163–168. >> doi: 10.1007/BF00415278

Tarasova, N.B., Gorshkov, O.V., Petrova, O.E., 2009. Nitratreduktaznaya aktivnost’ Desulfovibrio desulfuricans BKM 1388 [Nitrate reductase activity of Desulfovibrio desulfuricans BKM 1388]. Mikrobioligiya 78(2), 192–196 (in Russian).

How to Cite
Dorosh, L. S., Peretyatko, T. B., & Gudz, S. P. (2015). The patterns of utilization of sulfate and nitrate ions by bacteria Desulfomicrobium sp. CrR3 and Desulfovibrio desulfuricans Ya-11. Regulatory Mechanisms in Biosystems, 6(2), 156-160.