Glutathione metabolism system under condition of transplantation of muscle tissue in rats

  • O. V. Kulibaba Mechnykov Odessa National University
  • S. M. Kozishkurt Mechnykov Odessa National University
  • O. O. Duzenko Odessa National Medical University
  • I. L. Vovchuk Mechnykov Odessa National University
  • S. A. Petrov Mechnykov Odessa National University
Keywords: transplantation, reduced glutathione, glutathione reductase, glutathione peroxidase


The paper presents the results of determining the amount of reduced glutathione, glutathione reductase and glutathione peroxidase activity at transplantation of muscle tissue taken from rats from the same litter. During the work 2 surgical interventions were carried out: transplantation of muscle tissue taken from the same litter of rats, and operation without replanting. Transplantation was performed on nonlinear white male rats weighing 180–300 g. The animals were taken out of the experiment by passing electric current through the medulla oblongata. For donor transplants males from the same litter were used. In adult donor rats abdominal muscle tissue was extracted and transplanted to homologous tissue of the recipient rat. The same procedure was carried out with femoral muscle tissue. Operation without replanting was used to compare the effects of changes in the number of investigated parameters of the surgical exposure. Tissue not subject to any surgery served as a control. Parameters under study were determined on the first, third and seventh day after transplantation. Glutathione levels in tissues were determined byE. Butler, A. Dyubona, B. Kelly. Determination of glutathione reductase is based on the reduction of NADPH registration. Glutathione peroxidase activity was determined by accumulation of oxidized glutathione. Surgery is known to lead to expression of oxidative stress in the organs operated. Special role in antioxidant protection of the body is given to thiols. Expressed hydrophilic properties provide their high content in water fraction of cells and ability to protect biologically important molecules (enzymes, nucleic acids, hemoglobin) from oxidative damage. Property of thiol compounds is known to inhibit both fermented and not fermented free radical oxidation. For the first time it was found that transplantation of muscle tissue taken from animals from the same litter lead to significant reduction of glutathione in the femoral muscle of the recipient and abdominal muscle tissue of the donor and recipient and to significant increase in glutathione reductase activity in the femoral muscle tissue of the donor and in the abdominal muscle tissue of the donor and recipient. 


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How to Cite
Kulibaba, O. V., Kozishkurt, S. M., Duzenko, O. O., Vovchuk, I. L., & Petrov, S. A. (2015). Glutathione metabolism system under condition of transplantation of muscle tissue in rats. Regulatory Mechanisms in Biosystems, 6(1), 23-27.