Glutathione metabolism system under condition of transplantation of muscle tissue in rats
AbstractThe paper presents the results of determining the amount of reduced glutathione, glutathione reductase and glutathione peroxidase activity at transplantation of muscle tissue taken from rats from the same litter. During the work 2 surgical interventions were carried out: transplantation of muscle tissue taken from the same litter of rats, and operation without replanting. Transplantation was performed on nonlinear white male rats weighing 180–300 g. The animals were taken out of the experiment by passing electric current through the medulla oblongata. For donor transplants males from the same litter were used. In adult donor rats abdominal muscle tissue was extracted and transplanted to homologous tissue of the recipient rat. The same procedure was carried out with femoral muscle tissue. Operation without replanting was used to compare the effects of changes in the number of investigated parameters of the surgical exposure. Tissue not subject to any surgery served as a control. Parameters under study were determined on the first, third and seventh day after transplantation. Glutathione levels in tissues were determined byE. Butler, A. Dyubona, B. Kelly. Determination of glutathione reductase is based on the reduction of NADPH registration. Glutathione peroxidase activity was determined by accumulation of oxidized glutathione. Surgery is known to lead to expression of oxidative stress in the organs operated. Special role in antioxidant protection of the body is given to thiols. Expressed hydrophilic properties provide their high content in water fraction of cells and ability to protect biologically important molecules (enzymes, nucleic acids, hemoglobin) from oxidative damage. Property of thiol compounds is known to inhibit both fermented and not fermented free radical oxidation. For the first time it was found that transplantation of muscle tissue taken from animals from the same litter lead to significant reduction of glutathione in the femoral muscle of the recipient and abdominal muscle tissue of the donor and recipient and to significant increase in glutathione reductase activity in the femoral muscle tissue of the donor and in the abdominal muscle tissue of the donor and recipient.
Adamszak, M., Zeiler, M., Dikow, R., 2002. Kidney and hypertension. Kidney Int. 61(80), 62–67. >> doi: 10.1046/j.1523-1755.61.s80.28.x
Anderson, O.K., Neldam, S., 1998. The antihypertensive effect and tolerability of candesartan cilexetil, a new generation angiotensin II antagonist in comparison with losartan. Blood Press. 7, 53–59.
Bulyakova, N.V., Azarova, V.S., 2009. Morfofynkcionalnue osobennosti timysa i mushechnuh regeneratov pri vozdeustvii lazernogo izlycheniya i alloplastiki myshechnoy tkani vzroslogo jivotnogo v oblast mushechnoy travmu [Morphological and functional characteristics of the thymus and muscle regenerates when exposed to laser radiation and alloplasty muscle tissue of adult animals to muscle injury]. Izvestiya FAH. Seriya Biologicheskaya 1, 18–26.
Ferdinand, K.C., 2001. Update in pharmacologic treatment of hypertension. Clin. Cardiol. 19, 601–621. >> doi: 10.1016/S0733-8651(05)70213-7
Ferrar, P., Hens, A., Marti, P., 2002. Additive antiproteinuric effect of combined ACE – inhibition and angiotensin II receptor blockade. J. Hypertens. 20(1), 125–130. >> doi:10.1097/00004872-200201000-00018
Goryachkovsky, A.M., 2005. Klinicheskaya biohimiya v laboratornoy diagnostice [Clinical biochemistry in laboratory diagnostics]. Medicina, Moscow (in Russian).
Havinson, V.H., Barinov, V.A., Arutynyan, A.V., Malinin, V.V., 2003. Svobodnoradikalnoe okislenie i starenie [Free radical oxidation and aging]. Nauka, Moscow (in Rassian).
Hebert, L.A., Wilmer, W.A., Falkenhain, M.E., 2001. Renoprotection: One or many therapies. Kidney Int. 59, 1211–1226. >> doi: 10.1046/j.1523-1755.2001.0590041211.x
Lebedeva, A.I., Muslimov, S.A., Musina, L.A., Shcherbakov, D.A., 2014. Regeneraciya skeletnoy mychechnoy tkani eksperimentalnih jivotnih, inducirovannaya biomaterialom Alloplant [Regeneration of skeletal muscle tissue of the experimental animals induced Alloplant biomaterial]. International Journal of Experimental Education 3, 68–71 (in Russian).
Lujnikov, E.A., Goldfarb, J.S., Musselnus, S.G., 2000. Detoksikacionnaya terapiya [Detoxification therapy]. Medicina, Moscow (in Russian).
Lujnikov, E.A., Dagaev, V.N., 1988. Kriticheskie rasstroystva gomeostaza pri ostruh otravleniyah [Critical disorder homeostasis in acute poisoning]. NII Skoroy Pomoshi 74, 5–14 (in Russian).
Menshikova, E.B., Lankin, V.Z., Zenkov, N.K., 2006. Okislitelniy stress. Prooksidantu i antioksidantu [Oxidative stress. Pro-oxidants and antioxidants]. Slovo, Moscow (in Russian).
Meerson, F.Z., 1981. Adaptaciya, stress i profilaktika [Adaptation, stress and prevention]. Nauka, Moscow (in Russian).
Pereslegina, I.A., 1989. Aktivnost antioksidantnuh fermentov sluni zdorovuh detey [The activity of antioxidant enzymes saliva of healthy children]. Laboratornoe Delo 11, 20–23 (in Russian).
Repin, V.S., 1996. Medicinskaya kletochnaya biologiya: Novue fundamentalnue i prikladnue issledovaniya [Medical cell biology: New fundamental and applied research]. Transplantaciya Fetalnih Tkaneu i Kletok Cheloveka 1, 19–27 (in Russian).
Rodionov, S.J., Plyaskin, K.P., Pak, N.A., Masucheva, V.I., 1996. Opyt primeneniya biologicheski aktivnyh soedineniy iz fetalnuh tkaney cheloveka v lechenii onkologicheskih zabolevaniy [Experience in the use of biologically active compounds from human fetal tissues in the treatment of cancer]. Transplantaciya Fetalnuh Tkaney i Kletok Cheloveka 1, 90–92 (in Russian).
Ruggenenti, P., Remuzzi, G., 2000. Nephropathy of type I and type II diabetes: Diverse pathophysiology, same treatment [Nefropatiya tipa I i diabeta II tipa: Razlichnaya patfiziologiya, takoy je rejum]. Nephrol. Dial. Transpl. 15, 1900–1902.
Wendell, P.Z., 1968. Distribution of glutathione reductase and detection of glutathione-cystine transhydrogenax in rat tissues. Biochim. Biophys. Acta 159, 179–181.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons «Attribution» 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.