The role of neuro-specific dihydropyrimidinase-related protein 2 (dpyl2) in spatial memory formation in teleosts

  • D. V. Garina I. D. Papanin Institute for Biology of Inland Waters
  • V. V. Bol’shakov I. D. Papanin Institute for Biology of Inland Waters
  • I. Y. Toropygin V. N. Orekhovich Research Institute of Biomedical Chemistry
  • A. A. Mekhtiev A. I. Karayev Institute of Physiology, National Academy of Sciences of Azerbaijan
  • A. M. Andreeva A. I. Karayev Institute of Physiology, National Academy of Sciences of Azerbaijan
Keywords: teleosts; serotonin-modulating anticonsolidation protein; SMAP; learning; spatial memory

Abstract

This article presents the results of an experiment on the influence of serotonin-modulating anticonsolidation protein (SMAP) on the spatial memory formation of juvenile goldfish Carassius auratus (L.) in a maze with food reinforcement. Three experimental fish groups were formed: (1) intact animals, (2) experimental group (fish injected ICV with SMAP in 24 h before the beginning of training; 2 μl, 1.5 mg·ml–1), (3) active control group (fish injected ICV with inactivated SMAP). Goldfishes of the experimental group demonstrated the lowest capability for spatial recognition: the maximum level of performance of the task was on 4th day of the training – 38%, while the values of this index in fishes of the control and intact groups were 70% and 63% respectively. In general, throughout the period of the training the average value of task performance was 16% in the SMAP-injected fish (in the control and intact groups – 42% and 53%, respectively). By using Ds-Na-polyacrylamide gel electrophoresis SMAP compositeon has been revealed. It was found that it consists of 10–12 protein components, among which four proteins dominated. They were identified by mass spectrometry MALDI-TOF: spectrin, dihydropyrimidinase-related protein 2 (DPYL2), tubulin and actin. It has been suggested that the most likely candidate responsible for the negative effects of SMAP on fish memory formation is DPYL2. It was hypothesized that anticonsolidation effect of SMAP is caused by the effect of DPYL2 which blocks the growth of axons or its cytostatic activity which leads to disorders in formation of new neurons in the brain as a result of learning.

References

Bakhshalieva, R. R., Mekhtiev, A. A., & Kasimov, R. Y. (2010). Participation of serotonin-modulated anticonsolidation protein in mediation of action of adverse factors on lipid peroxidation level in tissues of sturgeon fry Acipenser güldenstädti persicus. Journal of Evolutionary Biochemistry and Physiology, 46(5), 442–446.


Broglio, C., Gómez, A., Durán, E., Ocaña, F. M., Jiménez-Moya, F., Rodríguez, F., & Salas, C. (2005). Hallmarks of a common forebrain vertebrate plan: Specialized pallial areas for spatial, temporal and emotional memory in actinopterygian fish. Brain Research Bulletin, 66(4–6), 277–281.


Broglio, C., Rodríguez, F., Gómez, A., Arias, J. L., & Salas, C. (2010). Selective involvement of the goldfish lateral pallium in spatial memory. Behavioural Brain Research, 210(2), 191–201.


Cognato, G. P., Bortolotto, J. W., Blazina, A. R., Christoff, R. R., Lara, D. R., Vianna, M. R., & Bonan, C. D. (2012). Y-maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods. Neurobiology of Learning and Memory, 98(4), 321–328.


Csányi, V., Csizmadia, G., & Miklosi, A. (1989). Long-term memory and recognition of another species in the paradise fish. Animal Behavior, 37(6), 908–911.


Deng, W., Aimone, J. B., & Gage, F. B. (2010). New neurons and new memories: How does adult hippocampal neurogenesis affect learning and memory? Nature Reviews Neuroscience, 11(5), 339–350.


Gaikwad, S., Stewart, A., Hart, P., Wong, K., Piet, V., Cachat, J., & Kalueff, A. V. (2011). Acute stress disrupts performance of zebrafish in the cued and spatial memory tests: The utility of fish models to study stress-memory interplay. Behavioral Processes, 87(2), 224–230.


Garina, D. V., & Mekhtiev, А. А. (2014). Effect of serotonin-modulated anticon-solidation protein on formation of long-term memory in the learning model of active avoidance in the carp (Cyprinus carpio). Journal of Evolutionary Biochemistry and Physiology, 50(1), 49–56.


Gasanov, G. G., & Mekhtiev, A. A. (1991). Detection of serotonin-modulating protein fraction and study of its participation in organization of the passive avoiding behavior. Bulletin of Experimental Biology and Medicine, 112(7), 5–7.


Guseinov, S. B., & Mekhtiev, A. A. (2013). Studies of the role of serotonin-modulating anticonsolidation protein in memory formation in rats in a shuttle box. Neuroscience and Behavioral Physiology, 43(5), 551–556.


Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophag. Nature, 227(4), 680–685.


Mekhtiev, A. A. (2000). Detection of protein with anticonsolidation properties in the rat brain. Bulletin of Experimental Biology and Medicine, 130(8), 739–742.


Mekhtiev, A. A., Gaisina, A. A., Palatnikov, G. M., & Kasimov, R. Y. (2006). Decrease in activity of the serotoninergic system during mutagenesis. Bulletin of Experimental Biology and Medicine, 142(6), 611–613.


Mekhtiev, A. A., Gaisina, A. A., Voronezhskaya, E. E., Khabarova, M. Y., Gudratov, N. O., & Huseynov, S. B. (2016). Uchastie serotonin-moduliruemogo anticonsolidatsionnogo belka v regulyatsii razvitiya embrionov bol’shogo prudovika (Lymnaea stagnalis) i sarcomy l’yuisa u myshej gibridnoji liniji (FL C57B2/6 X DBA) [Engagement of serotonin-modulating anticonsolidation protein in regulation of embryogenesis of Lymnaea stagnalis and Lewis sarcoma in hybrid mice (FL C57B2/6 X DBA)]. Rossijskij Fiziologicheskij Zhurnal imeni I. M. Sechenova, 102(4), 490–499 (in Russian).


Mekhtiev, A. A., Kozyrev, S. A., Nikitin, V. P., & Sherstnev, V. V. (2003). Izbiratel'noe vliianie antitel k belku SMP-69 na aktivnost' komandnykh nejronov oboronitel'nogo povedenija vinogradnykh ulitok [Selective effect of the antibody to protein SMP-69 on activity of the defence behavior command neurons in grape snail]. Rossijskij Fiziologicheskij Zhurnal imeni I. M. Sechenova, 89, 389–396 (in Russian).


Mekhtiev, A. A., Panahova, E. N., Rashidova, A. F., & Guseinov, S. B. (2015). Engagement of serotonin-modulating anticonsolidation protein in memory formation and suppression of drug addiction and epileptic seizures. In: Li, M. D. (Ed.). New developments in serotonin research. Nova Science Publishers, New York. pp. 123–143.


Movsum-Zadeh, S. K., Mekhtiev, A. A., Mekhtiev, K. S., Telford, W. G., Gaisina, А. А., & Zey’nalov, S. K. (2013). Detoxikatsionnye svojstva serotonin-moduliruemogo anticonsolidatsionnogo belka v otnoshenii toxinov khimicheskoj i bacterial’noj prirody [Detoxic properties of serotonin-modulating anticonsolidation protein to toxins of chemical and bacterial origin]. Izvestiya NAN Azerbajdzhana. Seriya Biologicheskie i Meditsinskie Nauki, 68(1), 24–29 (in Russian).


Nakamura, F., Kalb, R. G., & Strittmatter, S. M. (2000). Molecular basis of semaphorine-mediated axon guidance. Journal of Neurobiology, 44(2), 219–229.


Portavella, M., Vargas, J. P., Torres, B., & Salas, C. (2002). The effects of telencephalic pallial lesions on spatial, temporal, and emotional learning in goldfish. Brain Research Bulletin, 57(3–4), 397–399.


Rodriguez, F., Durán, E., Gómez, A., Ocaña, F. M., Ávarez, E., Jiménez-Moya, F., Broglio, C., & Salas, C. (2005). Cognitive and emotional functions of the teleost fish cerebellum. Brain Research Bulletin, 66(4–6), 365–370.


Schmidt, E. F., & Strittmatter, T. M. (2007). The CRMP family of proteins and their role in Sema3A signaling. Advances in Experimental Medicine and Biology, 600, 1–11.


Sherstnev, V. V., Gruden', M. A., Golubeva, O. N., Solov’eva, O. A., & Aleksandrov, Y. I. (2015). Long-lived newly formed neurons in the mature brain are involved in the support of learning and memory processes. Journal of Neurochemistry, 9(1), 13–19.


Sherstnev, V. V., Yurasov, V. V., Storozheva, Z. I., Gruden', M. A., & Proshin, A. T. (2010). Neurogenesis and apoptosis in the mature brain during formation and consolidation of long-term memory. Journal of Neurochemistry, 4(2), 109–115.


Yau, S., Li, A., & So, K.-F. (2015). Involvement of adult hippocampal neurogenesis in learning and forgetting. Neural Plasticity, Article ID 717958.


Zion, B., Karplus, I., Grinshpon, J., Rosenfeld, L., & Barki, A. (2011). Periodic reinforcement of acoustically conditioned behavior in St. Peter’s fish, Sarotherodon galilaeus, for ranching purposes. Aquaculture, 315(3–4), 394–399. 

Published
2018-01-30
How to Cite
Garina, D. V., Bol’shakov, V. V., Toropygin, I. Y., Mekhtiev, A. A., & Andreeva, A. M. (2018). The role of neuro-specific dihydropyrimidinase-related protein 2 (dpyl2) in spatial memory formation in teleosts. Regulatory Mechanisms in Biosystems, 9(1), 11-14. https://doi.org/10.15421/021802