Impact of gibberelic acid and tebuconazole on formation of the leaf system and functioning of donor – acceptor plant system of solanaceae vegetable crops

  • V. H. Kuryata Mykhailo Kotsiubynskyi Vinnytsia State Pedagogical University
  • V. V. Rogach Вінницький державний педагогічний університет імені Михайла Коцюбинського
  • O. I. Buina Mykhailo Kotsiubynskyi Vinnytsia State Pedagogical University
  • O. V. Kushnir Mykhailo Kotsiubynskyi Vinnytsia State Pedagogical University
  • O. V. Buinyi Mykhailo Kotsiubynskyi Vinnytsia State Pedagogical University
Keywords: donor – acceptor system, gibberellins, retardants, photosynthesis, Capsicum annuum L., Lycopersicum esculentum L.

Abstract

We studied the comparable effect of gibberelic acid and tebuconazole on morphogenesis, mesostructure formation and redistribution of flows in sweet peppers and tomatoes. It has been found that the use of gibberelic acid and tebuconazole retardant during budding leads to increased plant productivity due to optimization of the structure and operation of the plants’ leaf apparatus. It was established that both gibberelic and antigibberelic tebuconazole drug stimulated the formation and functioning of the photosynthetic apparatus of peppers and tomatoes, but the mechanisms of this regulation were different. Increased photosynthetic activity of plants under the influence of gibberellin was determined primarily by the formation of more leaves and total leaf surface. When using tebuconazole retardant there was a significant restructuring of the organization of leaf mezostructure: the leaves were thickened by chlorenchyma proliferation, there was an increase in the volume of columnar parenchyma cells and linear dimensions of spongy parenchyma leaf cells. The surface density of leaves significantly increased, the chlorophyll content and nitrogen content (especially protein) also increased, compared with control variants and variants using gibberelin. Such a profound restructuring of the photosynthetic apparatus in plants under the actions of tebuconazole led to a significant increase in donor leaves function of peppers and tomatoes, which is an indicator of the growth of net productivity of photosynthesis – the highest among all the variants of the experiment. The results also show that increasing the chlorophyll phytocenotic index was more significant than the increase of leaf index: the tomatoes under the action of tebuconazole had a lower leaf index than in control options, but due to a higher chlorophyll index the crop productivity increased.Since during the fruiting period the costs of assimilates to the growth of vegetative organs are greatly reduced, optimization of photosynthetic apparatus in pepper and tomato plants led to the laying of more fruit per plant and increasing crop yield. The analysis of the mass ratio of the researched vegetative and fruit plants shows that the mass fraction of fruit (an acceptor sphere of plants during fruiting) under the action of both drugs increased. Thus in both variants of the experiment both the mass fraction and donor assimilates of leaves were higher. Apart from the main source of assimilates – the processes of photosynthesis, which intensified due to the formation of a larger area of leaf surface (variant with gibberelin) or optimization of mesostructure (variant with tebuconazole) it is probable that the formation and growth of the embryo occurred in part due to reutilization of carbohydrates from the vegetative plant organs in carpogenesis processes. 

References

Khan, A. L., Hamayun, M., Kang, S. M., Kim, Y. H., Jung, H. Y., Lee, J. H., & Lee, I. J. (2012). Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: An example of Paecilomyces formosus LHL10. BMC Microbiology, 12(1), 3.

Kumar, S., Ghatty, S., Satyanarayana, J., & Guha, A. (2012). Paclobutrazol treatment as a potential strategy for higher seed and oil yield in field-grown Camelina sativa L. Crantz. BSK Research Notes, 5, 1–13.

Kuryata, V. G., Rohach, V. V., Rohach, T. I., & Khranovska, T. V. (2016). The use of antigibberelins with different mechanisms of action on morphogenesis and production process regulation in the plant Solanum melongena (Solanaceae). Visnyk of Dnipropetrovsk University. Biology, Ecology, 24(1), 230–234.

Matysiak, K., & Kaczmarek, S. (2013). Effect of chlorocholine chloride and triazoles – tebuconazole and flusilazole on winter oilseed rape (Brassica napus var. oleifera L.) in response to the application term and sowing density. Journal of Plant Protection Research, 53(1) 79–88.

Mohammad, N. K., & Mohammad, F. (2013). Effect of GA3, N and P ameliorate growth, seed and fibre yield by enhancing photosynthetic capacity and carbonic anhydrase activity of linseed. Integrative Agriculture, 12(7), 1183–1194.

Muhammad, I., & Muhammad, A. (2013). Gibberellic acid mediated induction of salt tolerance in wheat plants: Growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environmental and Experimental Botany, 86, 76–85.

Panyapruek, S., Sinsiri, W., Sinsiri, N., Arimatsu, P., & Polthanee, A. (2016) Effect of paclobutrazol growth regulator on tuber production and starch quality of cassava (Manihot esculenta Crantz). Asian Journal of Plant Sciences, 15(1-2), 1–7.

Pavlista, A. D. (2013). Influence of foliar-applied growth retardants on russet burbank potato tuber production. American Journal of Potato Research, 90, 395–401.

Pobudkiewicz, A. (2014). Influence of growth retardant on growth and development of Euphorbia pulcherrima Willd. ex Klotzsch. Acta Agrobotanica, 67(3), 65–74.

Poprotska, I. V., & Kuryata, V. G. (2017). Features of gas exchange and use of reserve substances in pumpkin seedlings in conditions of skoto- and photomorphogenesis under the influence of gibberellin and chlormequat-chloride. Regulatory Mechanisms in Biosystems, 8(1), 71–76.

Ribeiro, D. M., Araújo, W. L., Fernie, A. R., Schippers, J. H. M., & Mueller-Roeber, B. (2012). Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis. Journal of Experimental Botany, 63(7), 2769–2786.

Rogach, V. V., & Rogach, T. I., 2015. Vplyv syntetychnyh stymuljatoriv rostu na morfofiziologichni harakterystyky ta biologichnu produktyvnist’ kul’tury kartopli. Visnyk of Dnipropetrovsk University. Biology, Ecology, 23(2), 221–224 (in Ukrainian).

Rogach, V. V., Poprotska, I. V., & Kuryata, V. G. (2016). Diya giberelinu ta retardantiv na morfogenez, fotosyntetychnyj aparat і produktyvnist’ kartopli [Effect of gibberellin and retardants on morphogenesis, photosynthetic apparatus and productivity of the potato]. Visnyk of Dnipropetrovsk University. Biology, Ecology, 24(2), 416–419 (in Ukrainian).

Saeidi-Sar, S., Abbaspour, H., Afshari, H., & Yaghoobi, S. R. (2013). Effects of ascorbic acid and gibberellin A3 on alleviation of salt stress in common bean (Phaseolus vulgaris L.) seedlings. Acta Physiologiae Plantarum, 35(3), 667–677.

Sardoei, A. S., Yazdi, M. R., & Shshdadneghad, M. (2014). Effect of cycocel on growth retardant cycocel on reducing sugar, malondialdehyde and other aldehydes of Cannabis sativa L. International Journal of Biosciences, 4(6), 127–133.

Sugiura, D., Sawakami, K., Kojim, M., Sakakibara, H., Terashima, I., & Tateno, M. (2015). Roles of gibberellins and cytokinins in regulation of morphological and physiological traits in Polygonum cuspidatum responding to light and nitrogen availabilities. Functional Plant Biology, 42(4), 397–409.

Sukul, А., Sukul, N. C., Sen, P., Bhattacharya, A., & Sukul, S. (2014). Homeopathic potencies alter photosynthesis of cowpea. Homeopathy, 103(1), 91–94.

Tae-Yun, K., & Jung-Hee, H. (2012). Effects of hexaconazole on growth and antioxidant potential of cucumber seedlings under UV-B radiation. Environmental Sciences, 21(12), 1435–1447.

Van Emden, H. F. (2008). Statistics for terrified biologists. Blackwell, Oxford.

Wang, X., Han, F., Yang, M., Yang, P., & Shen, S. (2013). Exploring the response of rice (Oryza sativa) leaf to gibberellins: A proteomic strategy. Rice, 6(1), 17.

Wang, Y., Gu, W., Xie, T., Li, L., Sun, Y., Zhang, H., Li, J., & Wei, S. (2016). Mixed compound of DCPTA and CCC increases maize yield by improving plant morphology and up-regulating photosynthetic capacity and antioxidants. Plos One, 1, 25.

Yan, W., Yanhong, Y., Wenyu, Y., Taiwen, Y., Weiguo, L., & Wang, X. (2013). Responses of root growth and nitrogen transfer metabolism to uniconazole, a growth retardant, during the seedling stage of soybean under relay strip. Communications in Soil Science and Plant Analysis Intercropping System, 44(22), 3267–3280.

Yan, Y., Wan, Y., Liu, W., Wang, X., Yong, T., & Yang, W. (2015). Influence of seed treatment with uniconazole powder on soybean growth, photosynthesis, dry matter accumulation after flowering and yield in relay strip intercropping system. Plant Production Science, 18(3), 295–301.

Yang, L., Yang, D., Yan, X., Cui, L., Wang, Z., & Yuan, H. (2016). The role of gibberellins in improving the resistance of tebuconazole-coated maize seeds to chilling stress by microencapsulation. Scientific Reports, 60, 1–12.

Yu, S. M., Lo, S. F., & Ho, T. D. (2015) Source-sink communication: Regulated by hormone, nutrient, and stress cross-signaling. Trends in Plant Science, 20(12), 844–857.

Zhang, W., Xu, F., Hua, C., & Cheng, S. (2013). Effect of chlorocholine chloride on chlorophyll, photosynthesis, soluble sugar and flavonoids of Ginkgo biloba. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 97–103.

Published
2017-04-29
How to Cite
Kuryata, V. H., Rogach, V. V., Buina, O. I., Kushnir, O. V., & Buinyi, O. V. (2017). Impact of gibberelic acid and tebuconazole on formation of the leaf system and functioning of donor – acceptor plant system of solanaceae vegetable crops. Regulatory Mechanisms in Biosystems, 8(2), 162-168. https://doi.org/10.15421/021726