@article{Yuskiv_Yuskiv_2021, title={The synthesis of lipids and proteins in vitro in tissues of Cyprinus carpio infected with Bothriocephalus acheilognathi }, volume={12}, url={https://medicine.dp.ua/index.php/med/article/view/692}, DOI={10.15421/022112}, abstractNote={<p>The problem of the mechanisms of regulation of biochemical processes in carp <em>Cyprinus carpio</em> (Linnaeus, 1758) tissues and organs caused by infection with <em>Bothriocephalus</em> (<em>Schyzocotyle</em>) <em>acheilognathi</em> (Yamaguti, 1934) at different intensities of invasion remains practically unstudied. The purpose of this study was to dedetrmine the intensity of lipid and protein synthesis in vitro when [6-<sup>14</sup>C]glucose and [2-<sup>14</sup>C]lysine are used as their precursor in the tissues of the intestine, hepatopancreas and skeletal muscles of carp. The study was conducted on this-year carp with body weight 14.5–20.5 g, at different invasion rates of the helminth <em>B. acheilognathi</em>, which belongs to the family Bothriocephalidae of the Pseudophyllidae order of the Cestoda class of the Plathelminthes phylum. The examined carp were divided into three groups: 1st group of fish was free from intestinal helminths of <em>B. acheilognathi</em> (control); 2nd group of fish was weakly infected with helminths (intensity of invasion was 1–3 helminths per fish); the 3rd group of fish was highly infected (the invasion intensity was 4 worms and more per fish). Our results showed that in fish infected with the helminth <em>B. acheilognathi</em> in comparison to uninfected, the intensity of lipid synthesis in the intestinal wall, hepatopancreas, skeletal muscle was much lower when [6-<sup>14</sup>C]glucose was used as a predecessor than when [2-<sup>14</sup>C]lysine was used as a predecessor. In the examined tissues, significant decrease was observed in the synthesis of reserve lipids (mono-, di- and triacylglycerols) in comparison to the structural (phospholipids and cholesterol), which depends on the intensity of the <em>B. acheilognathi</em> invasion. In the metabolic processes in the wall of the intestine, hepatopancreas, skeletal muscle of this-year carp infectd with <em>B. acheilognathi</em> helminths, under in vitro conditions, [6-<sup>14</sup>C]glucose was used more than [2-<sup>14</sup>C]lysine. The intensity of protein synthesis in the intestinal wall, hepatopancreas, skeletal muscles of this-year carp infected with the helminth <em>B.&nbsp;acheilognathi</em> under <em>in vitro</em> conditions increased when [6-<sup>14</sup>C]glucose was added to the incubation medium, on average 7.1–28.3% and decreased when [2-<sup>14</sup>C]lysine was added, on average 7.8–25.7%.</p&gt;}, number={1}, journal={Regulatory Mechanisms in Biosystems}, author={Yuskiv, L. L. and Yuskiv, I. D.}, year={2021}, month={Mar.}, pages={78-85} }