@article{Brygadyrenko_Lieshchova_Bilan_Tishkina_Horchanok_2019, title={Effect of alcohol tincture of Aralia elata on the organism of rats and their gut microbiota against the background of excessive fat diet }, volume={10}, url={https://medicine.dp.ua/index.php/med/article/view/573}, DOI={10.15421/021973}, abstractNote={<p>Pharmacological effects of the medicinal plant <em>Aralia elata</em> (Miq.) Seem. 1868 (Araliaceae) are related to presence of over 150 secondary metabolites, including flavonoids, sterols, polysaccharides, terpenoid saponins and terpenoid acids, though the main biologically active substances of <em>A. elata</em> are saponins and flavonoids. Some clinical tests and experimental studies have proved the influence of <em>A. elata</em> on the organism through increase in physical work capacity, exerting an anti-stress effect against a broad range of harmful factors, including stress from cold, immobilization, ultraviolet radiation and low pressure. The literature also reports the anti-ulcer, anti-secretory, antifungal, anti-tumour, and antimicrobial activity of preparations from this plant. In&nbsp;our laboratory experiment, we determined that ethanolic tincture of <em>A. elata</em> has no effect on the intensity of growth of body weight of young rats against the background of excessive content of fat in their diet. Excessive fat-feeding of male rats leads mostly to disorders in the functioning of the liver and development of steatosis. This was accompanied by reduction in relative mass of the liver, increase in activity of hepatic enzymes, dysproteinemia, increase in the level of bilirubin and decrease in the level of urea. Additional use of 0.1% ethanol led to impaired functioning of the kidneys, reduction of their relative mass, signs of dehydration, increase in the level of creatinine and total calcium in the blood. Use of ethanolic tincture of <em>A. elata</em> mitigates negative excess of fat, is accompanied by normalization of indices of mass of the organs, less notable dysproteinemia, impairment in the level of creatinine, glucose, urea, cholesterol, bilirubin and total calcium. Ethanolic tincture of <em>A. elata</em> has a low immunosuppressive action, against the background of a high fat diet it leads to increase in the amount of typical <em>Escherichia сoli</em>, decrease in <em>Еnterococcus</em> spp. and <em>Enterobacter</em> spp., significant decrease and in high concentrations (0.1% ethanolic tincture of <em>A. elata</em>) elimination of bacteria of <em>Clostridium</em> and <em>Klebsiella</em> genera, and also various yeast fungi in the intestine. In the examined male rats, against the background of excess of fat in the diet, no serious changes in the composition of the normal gut microbiota (<em>Bifidobacterium</em> spp., <em>Lactobacillus</em> spp., <em>Proteus</em> spp., <em>Staphylococcus</em> spp., <em>Candida</em> spp.) was observed, nor were any lactose-negative enterobacteria (<em>Citrobacter</em> genus) found. Perspectives of further research include determining histological, histochemical and immune-histological changes in the organs of laboratory animals under the effect of ethanolic tincture of <em>A. elata</em> following excessive accumulation of fat.</p&gt;}, number={4}, journal={Regulatory Mechanisms in Biosystems}, author={Brygadyrenko, V. V. and Lieshchova, M. A. and Bilan, M. V. and Tishkina, N. M. and Horchanok, A. V.}, year={2019}, month={Oct.}, pages={497-506} }