@article{Drehval_Dreus_Cherevach_Sklyar_2018, title={Strains of soil microorganisms promising for the creation of a complex plant protection product against mycoses and harmful insects}, volume={9}, url={https://medicine.dp.ua/index.php/med/article/view/406}, DOI={10.15421/021809}, abstractNote={<p>We evaluated the antagonistic activity of 23 strains of <em>Bacillus</em> spр. against phytopathogenic fungi <em>Fusarium oxysporum</em>, <em>F. culmorum</em>, <em>F. moniliforme</em>, <em>Cladosporium herbarum</em>, <em>Alternaria alternata</em> and <em>Aspergillus niger</em>. The antagonistic activity was tested by agar diffusion (the method of blocks). For determining the influence of bacteria on barley plants , ardent seeds were treated by cultural liquid (dilution 1 : 10) for 2 hours and germinated in Petri dishes on moist filter paper. The fungistatic effect of <em>Bacillus</em> sрp. separately and in combination with entomopathogens (in equal ratio) was determined by the level of inhibition of the fungi <em>Fusarium</em> spp. on a solid nutrient medium with 5% of the culture liquid. Insecticidal activity of microorganisms was determined in the model experiments by the percentage of death of the caterpillar <em>Archips podana</em> Scop. Strains of <em>Bacillus</em> sp. KMB-3 and <em>Bacillus</em> sp. KMB-6 inhibited the growth of all test cultures (zones of growth inhibition 11.4–30.6 and 11.5–29.4 mm, respectively). We established the absence of antagonism between selected strains and entomopathogenic bacteria <em>Bacillus thuringiensis</em> IMB-7186, fungi <em>Beauveria bassiana</em> IMB-F-100043. We found that treatment of barley seeds with culture liquids of <em>Bacillus</em> sp. KMB-3 and <em>Bacillus</em> sp. KMB-6 didn’t have a negative effect on the morphometric indices and dry weight of seedlings. We&nbsp;established that the highest percentage of growth inhibition of <em>F.&nbsp;culmorum</em> IMB-F-50716 was provided by a complex of <em>Bacillus</em> sp. KMB-3, <em>B. bassiana</em> IMB-F-100043 and <em>B.&nbsp;thuringiensis</em> IMB-7186, whose action was at the same level as the action of monoculture <em>Bacillus</em> sp. KMB-3 (85.4% and 84.7%, respectively). The highest percentage inhibition of growth of <em>F. oxysporum</em> ІМВ-F-54201 was provided by a complex of strains of <em>Bacillus</em> sp. KMB-3 and <em>B. bassiana</em> IMB-F-100043, whose effect was slightly inferior to that of the monoculture <em>Bacillus</em> sp. KMB-3 (68.4% and 75.1%, respectively). The insecticidal activity of complexes <em>Bacillus</em> sp. KMB-3, <em>B. bassiana</em> IMB-F-100043, <em>B. thuringiensis</em> IMB-7186 or <em>Bacillus</em> sp. KMB-6, <em>B. bassiana</em> IMB-F-100043, <em>B.&nbsp;thuringiensis</em> IMB-7186 insignificantly differed from that of the complex entomopathogens <em>B. bassiana</em> IMB-F-100043 and <em>B. thuringiensis</em> IMB-7186 (71.1%, 73.3% death versus 80.0%). The&nbsp;selected microbial complexes can be considered as promising for the development of a preparation for the protection of plants against fungal diseases and harmful insects.</p&gt;}, number={1}, journal={Regulatory Mechanisms in Biosystems}, author={Drehval, O. A. and Dreus, A. A. and Cherevach, N. V. and Sklyar, T. V.}, year={2018}, month={Feb.}, pages={69-74} }