@article{Kovalchuk_Dovban_Kanga_Ushakovа_2015, title={Short-term effect of adrenalin on S-100b and N-CAM level in the different rat brain areas}, volume={6}, url={https://medicine.dp.ua/index.php/med/article/view/021522}, DOI={10.15421/021522}, abstractNote={<p class="00Annotation"><span lang="UK">The level of adrenalin </span><span lang="EN-US">grows</span><span lang="UK"> under stress condition</span><span lang="EN-US">s</span><span lang="UK">, sense of danger, anxiety, fear, trauma, burns and shock. In high concentrations adrenaline increases the speed of protein catabolism. Working through the circulatory system, adrenaline affects almost all the functions of organs, causing the body mobiliz</span><span lang="EN-US">ation </span><span lang="UK">to counter stressful situations. Due to ELISA the astrocytes-specific protein (S-100b) and neural cell adhesion molecule (N-CAM) were studied</span><span lang="EN-US">.</span><span lang="UK"> S</span><span lang="EN-US">-</span><span lang="UK">100b is produced mainly by astrocytes іn the brain and depend</span><span lang="EN-US">ing</span><span lang="UK"> on the concentration </span><span lang="EN-US">it </span><span lang="UK">cause</span><span lang="EN-US">s</span><span lang="UK"> trophic or toxic effect </span><span lang="EN-US">on </span><span lang="UK">the neurons and glial cells.</span><span lang="UK">Strong stress and ischemia induce re</span><span lang="EN-US">-</span><span lang="UK">distribution of calcium-binding protein S-100b and elevation of its level. Quantitative changes of S-100b </span><span lang="EN-US">under </span><span lang="UK">the influence of various factors on the body </span><span lang="EN-US">which </span><span lang="UK">lead to the metabolic disorder in the brain </span><span lang="EN-US">are</span><span lang="UK"> considered today as a sign of brain damage (cortical, ischemic</span><span lang="EN-US"> one</span><span lang="UK">, etc.). Fluctuations in the concentration of S-100b in the brain are not always accompanied by marked deterioration of the physical condition of animals, but </span><span lang="EN-US">they </span><span lang="UK">can also lead to a </span><span lang="EN-US">number</span><span lang="UK"> of violations of integrative functions of the brain depending on over</span><span lang="EN-US">-</span><span lang="UK">production of this protein. Most N-CAM are transmembrane proteins that cross the plasma membrane</span><span lang="UK">once</span><span lang="EN-US">; </span><span lang="UK">intracellular domains </span><span lang="EN-US">have </span><span lang="UK">different size and </span><span lang="EN-US">it is thought </span><span lang="UK">they are involved in binding to cytoskeleton or cell signaling. Violation of N-CAM functions leads to disruption of nerve sprouts. Data</span><span lang="EN-US"> o</span><span lang="UK">btained in our study showed no serio</span><span lang="EN-US">u</span><span lang="UK">s re</span><span lang="EN-US">-</span><span lang="UK">distribution of S-100b and N-CAM level in the different areas of rat brain (cerebral cortex, hippocampus and thalamus) under effect of adrenalin administered to the animals (under skin) in dosage of 0.45–0.60 mg per rat, 1 time per day during 10 days, probably because of the type of injection and/or short time of adrenalin action. </span><span lang="EN-US">Increased </span><span lang="UK">dosage of adrenaline 1 hour before decapitation lead</span><span lang="EN-US">s</span><span lang="UK"> to the decrease</span><span lang="EN-US"> of</span><span lang="UK"> level of total protein in membrane fraction extracted from brain tissue without changing </span><span lang="EN-US">the </span><span lang="UK">level of S-100b and N-CAM. </span></p&gt;}, number={2}, journal={Regulatory Mechanisms in Biosystems}, author={Kovalchuk, Y. P. and Dovban, O. O. and Kanga, A. M. and UshakovаG. A.}, year={2015}, month={Sep.}, pages={119-124} }