Genotype-mutagenic interaction in the cytogenetic variability of winter wheat for a new ecogenetic factor
Keywords:
cereals; chromosomal abnormalities; sodium azide; pollen sterility; cytogenetic analyze; site-specific activity; mutagenesis.
Abstract
The study of cytogenetic parameters of the activity of new mutagenic factors aims to reliably establish the possibilities of these factors in terms of variability depending on the subject of mutagenic action, the optimal use of certain factor sizes, the combination of the selected protocol with optimization of the yield of mutant forms in the future. Seeds of eight varieties of winter wheat (Balaton, Borovytsia, Zeleny Gai, Zoloto Ukrainy, Kalancha, Niva Odeska, Polyanka, Pochayna) were treated by SA (sodium azide) at concentrations of 0.010%, 0.025%, 0.05%, 0.10%. They were soaked in water solution for 24 hours. Cytogenetic activity was evaluated by pollen sterility, evaluation of general rates and indicators of spectra of chromosomal abnormalities at medium phases of cell mitosis according to wheat variety and chemical agent concentrations. As a result of the study, the key importance of the genotype-mutagenic interaction was demonstrated within the limits of variability of the main indicators of the frequency and spectrum of chromosomal aberrations. It has been established that in the future it will be more optimal to use two varieties whose genotype-mutagenic specificity indicators are significantly higher and one should expect a more significant yield of promising mutant forms from them in the future, especially in combination with SA concentrations in the range of 0.025% and 0.05%. It is demonstrated that the main parameters that reflect genetically determined possibilities in susceptibility to the ecogenetic factor are pollen fertility, the overall frequency of chromosome aberrations, and the number of induced fragments. The use of other parameters displays the trend only partially or does not display it at all, as is the case with the use of rarer types of chromosomal rearrangements. The least promising forms have also been identified for use as starting material in treatments with this substance. It is demonstrated that, in general, this agent is characterized by the same patterns in the induction of cytogenetic activity as for other chemical supermutagens, with some variations depending on the starting material. In the future, it is planned to link the obtained data with the frequency and quality of the resulting hereditary changes, primarily complex biochemical and physiological ones, in order to improve the quality of plant products and various types of plant tolerance to adverse environmental conditions.References
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Beiko, V., & Nazarenko, M. (2022). Early depressive effects of epimutagen in the first generation of winter wheat varieties. Agrology, 5(2), 43–48.
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Hase, Y., Satoh, K., Seito, H., & Oono, Y. (2020). Genetic consequences of acute/ chronic gamma and carbon ion irradiation of Arabidopsis thaliana. Frontiers in Plant Science, 11, 336.
Hintzsche, H., Hemmann, U., Poth, A., Utesch, D., Lott, J., & Stopper, H. (2017). Fate of micronuclei and micronucleated cells. Mutation Research, 771, 85–98.
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Horshchar, V., & Nazarenko, M. (2022). Inhibition of mutagenic effect in winter wheat as a result of ethylmethansulfonat action. Agrology, 5(3), 75–80.
Jalal, A., Oliveira, J., Ribeiro, J., Fernandes, G., Mariano, G., Trindade, V., & Reis, A. (2021). Hormesis in plants: Physiological and biochemical responses. Ecotoxicology and Environmental Safety, 207, 111225.
Navid, S., Soufizadeh, S., Jahansuz, M., & Eskandari, A. (2021). Gamma radiation influence on germination characteristics of barley. Dysona-Applied Science, 2(1), 8–12.
Nazarenko, M. (2020). Induction of winter wheat plant structure mutations by chemomutagenesis. Agrology, 3(1), 57–65.
Nazarenko, M., & Izhboldin, O. (2017). Chromosomal rearrangements caused by gamma-irradiation in winter wheat cells. Biosystems Diversity, 25(1), 25–28.
Nazarenko, M., Izhboldin, O., & Izhboldina, O. (2022). Study of variability of winter wheat varieties and lines in terms of winter hardness and drought resistance. AgroLife Scientific Journal, 11(2), 116–123.
Nazarenko, M., Mykolenko, S., & Chernysky, V. (2019). Modern ukrainian winter wheat varieties grain productivity and quality at ecological exam. Agriculture and Forestry, 65(1), 127–136.
Pekol, S., Baloglu, M., & Celik, A. (2016). Evaluation of genotoxic and cytologic effects of environmental stress in wheat species with different ploidy levels. Turkish Journal of Biology, 40, 580–588.
Ram, H., Soni, P., Salvi, P., Gandass, N., Sharma, A., Kaur, A., & Sharma, T. (2019). Insertional mutagenesis approaches and their use in rice for functional genomics. Plants, 8(9), 310.
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Shabani, M., Alemzadeh, A., Nakhoda, B., Razi, H., Houshmandpanah, Z., & Hildebrand, D. (2022). Optimized gamma radiation produces physiological and morphological changes that improve seed yield in wheat. Physiology Molecular Biology Plants, 28(8), 1571–1586.
Spencer-Lopes, M., Forster, B., & Jankuloski, L. (2018). Manual on mutation breeding. Third edition. Food and Agriculture Organization of the United Nations, Rome.
Von Well, E., Booyse, M., & Fossey, A. (2023). Gamma irradiation as tool for mutation breeding in wheat. In: Wanyera, R. O., & Wamalwa, M. (Eds.). Wheat. IntechOpen, London.
Von Well, E., Fossey, A., & Booyse, M. (2022). Effect of gamma irradiation on nucleolar activity, an indicator of metabolic activity, in root tip cells of tetraploid Triticum turgidum ssp. durum L. Protoplasma, 259, 453–468.
Von Well, E., Fossey, A., & Booyse, M. (2023). The relationship of the efficiency of energy conversion into growth as an indicator for the determination of the optimal dose for mutation breeding with the appearance of chromosomal abnormalities and incomplete mitosis after gamma irradiation of kernels of Triticum turgidum ssp. durum L. Radiation and Environmental Biophysics, 62, 195–212.
Zhao, H., Zhuang, Y., Li, R., Liu, Y., Mei, Z., He, Z., Zhou, F., & Zhou, Y. (2019). Effects of different doses of X-ray irradiation on cell apoptosis, cell cycle, DNA damage repair and glycolysis in HeLa cells. Oncology Letters, 17, 42–45.
Aly, A., Maraei, R., & Aldrussi, I. (2019). Changes in peroxidase and polyphenol oxidase activity and transcript levels of related genes in two egyptian bread wheat cultivars (Triticum aestivum L.) affected by gamma irradiation and salinity stress. Bangladesh Journal of Botany, 48, 177–186.
Amri-Tiliouine, W., Laouar, M., Abdelguerfi, A., Jankowicz-Cieslak, J., Jankuloski, L., & Till, B. J. (2018). Genetic variability induced by gamma rays and preliminary results of low-cost Tilling on M2 generation of Chickpea (Cicer arietinum L.). Frontiers in Plant Science, 9, 1568.
Balkan, A., Bilgin, O., Başer, I., Göçmen, D., Demirkan, A., & Deviren, B. (2019). Improvement of grain yield and yield associated traits in bread wheat (Triticum aestivum L.) genotypes through mutation breeding using gamma irradiation. Journal of Tekirdag Agricultural Faculty, 16, 103–111.
Beiko, V., & Nazarenko, M. (2022). Early depressive effects of epimutagen in the first generation of winter wheat varieties. Agrology, 5(2), 43–48.
Beyaz, R., Telci Kahramanogullari, C., Yildiz, C., Darcin, E. S., & Yildiz, M. (2016). The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions. Journal of Environmental Radioactivity, 162–163, 129–133.
Bhat, T., & Wani, A. (Eds.). (2017). Chromosome structure and aberrations. Springer, New Delhi.
Bilgın, O., Sarier, S., Başer, İ., & Balkan, A. (2022). Enhancement of androgenesis and plant regeneration from wheat anther culture by seed pre-sowing gamma irradiation. Journal of Tekirdag Agricultural Faculty, 19, 354–365.
Bondarenko, M., & Nazarenko, M. (2020). French breeding wheat varieties adaptabiliy for the Ukrainian North Steppe conditions. Agrology, 3(4), 193–198.
Chernysky, V., & Gumentyk, M. (2020). Innovative principles of selection of valuable genotypes in the system of competitive strain testing. Agrology, 3(4), 219–224.
Dwinanda, P., Syukur, S., & Suliansyah, I. (2020). Induction of mutations with gamma ray radiation to improve the characteristics of wheat (Triticum aestivum L.) genotype IS-Jarissa. IOP Conference Series: Earth and Environmental Science, 497, 012013.
El-Mouhamady, A., & Ibrahim, H. (2020). Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation. Bulletin of the National Research Centre, 44, 108.
Ergün, N., Akdoğan, G., Ünver İkincikarakaya, S., & Aydoğan, S. (2023). Determination of optimum gamma ray irradiation doses for hulless barley (Hordeum vulgare var. nudum L. Hook. f.) genotypes. Yuzuncu Yil University Journal of Agricultural Sciences, 33, 219–230.
Gupta, S., Datta, A. K., Pramanik, A., Biswas, J., & Karmakar, R. (2019). X-ray and gamma irradiation induced chromosomal aberrations in plant species as the consequence of induced mutagenesis – an overview. Plant Archives, 19(2), 1973–1979.
Handa, H., Kanamori, H., Tanaka, T., Murata, K., Kobayashi, F., Robinson, S., Koh, C., Pozniak, C., Sharpe, A., Paux, E., Wu, J., & Nasuda, S. (2018). Structural features of two major nucleolar organizer regions (NORs), nor-B1 and nor-B2, and chromosome-specific rRNA gene expression in wheat. The Plant Journal, 96, 1148–1159.
Hase, Y., Satoh, K., Seito, H., & Oono, Y. (2020). Genetic consequences of acute/ chronic gamma and carbon ion irradiation of Arabidopsis thaliana. Frontiers in Plant Science, 11, 336.
Hintzsche, H., Hemmann, U., Poth, A., Utesch, D., Lott, J., & Stopper, H. (2017). Fate of micronuclei and micronucleated cells. Mutation Research, 771, 85–98.
Hong, M., Kim, D., Jo, Y., Choi, H.-I., Ahn, J.-W., Kwon, S.-J., Kim, S., Seo, Y., & Kim, J.-B. (2022). Biological effect of gamma rays according to exposure time on germination and plant growth in wheat. Applied Sciences, 12(6), 3208.
Horshchar, V., & Nazarenko, M. (2022). Cytogenetic effects of low-damaging chemical supermutagen action on winter wheat samples. Agrology, 5(4), 116–121.
Horshchar, V., & Nazarenko, M. (2022). Inhibition of mutagenic effect in winter wheat as a result of ethylmethansulfonat action. Agrology, 5(3), 75–80.
Jalal, A., Oliveira, J., Ribeiro, J., Fernandes, G., Mariano, G., Trindade, V., & Reis, A. (2021). Hormesis in plants: Physiological and biochemical responses. Ecotoxicology and Environmental Safety, 207, 111225.
Navid, S., Soufizadeh, S., Jahansuz, M., & Eskandari, A. (2021). Gamma radiation influence on germination characteristics of barley. Dysona-Applied Science, 2(1), 8–12.
Nazarenko, M. (2020). Induction of winter wheat plant structure mutations by chemomutagenesis. Agrology, 3(1), 57–65.
Nazarenko, M., & Izhboldin, O. (2017). Chromosomal rearrangements caused by gamma-irradiation in winter wheat cells. Biosystems Diversity, 25(1), 25–28.
Nazarenko, M., Izhboldin, O., & Izhboldina, O. (2022). Study of variability of winter wheat varieties and lines in terms of winter hardness and drought resistance. AgroLife Scientific Journal, 11(2), 116–123.
Nazarenko, M., Mykolenko, S., & Chernysky, V. (2019). Modern ukrainian winter wheat varieties grain productivity and quality at ecological exam. Agriculture and Forestry, 65(1), 127–136.
Pekol, S., Baloglu, M., & Celik, A. (2016). Evaluation of genotoxic and cytologic effects of environmental stress in wheat species with different ploidy levels. Turkish Journal of Biology, 40, 580–588.
Ram, H., Soni, P., Salvi, P., Gandass, N., Sharma, A., Kaur, A., & Sharma, T. (2019). Insertional mutagenesis approaches and their use in rice for functional genomics. Plants, 8(9), 310.
Rozman, L. (2015). The effect of gamma radiation on seed germination of barley (Hordeum vulgare L.). Acta Agriculturae Slovenica, 103(2), 307–311.
Shabani, M., Alemzadeh, A., Nakhoda, B., Razi, H., Houshmandpanah, Z., & Hildebrand, D. (2022). Optimized gamma radiation produces physiological and morphological changes that improve seed yield in wheat. Physiology Molecular Biology Plants, 28(8), 1571–1586.
Spencer-Lopes, M., Forster, B., & Jankuloski, L. (2018). Manual on mutation breeding. Third edition. Food and Agriculture Organization of the United Nations, Rome.
Von Well, E., Booyse, M., & Fossey, A. (2023). Gamma irradiation as tool for mutation breeding in wheat. In: Wanyera, R. O., & Wamalwa, M. (Eds.). Wheat. IntechOpen, London.
Von Well, E., Fossey, A., & Booyse, M. (2022). Effect of gamma irradiation on nucleolar activity, an indicator of metabolic activity, in root tip cells of tetraploid Triticum turgidum ssp. durum L. Protoplasma, 259, 453–468.
Von Well, E., Fossey, A., & Booyse, M. (2023). The relationship of the efficiency of energy conversion into growth as an indicator for the determination of the optimal dose for mutation breeding with the appearance of chromosomal abnormalities and incomplete mitosis after gamma irradiation of kernels of Triticum turgidum ssp. durum L. Radiation and Environmental Biophysics, 62, 195–212.
Zhao, H., Zhuang, Y., Li, R., Liu, Y., Mei, Z., He, Z., Zhou, F., & Zhou, Y. (2019). Effects of different doses of X-ray irradiation on cell apoptosis, cell cycle, DNA damage repair and glycolysis in HeLa cells. Oncology Letters, 17, 42–45.
Published
2023-07-30
How to Cite
Horshchar, V., & Nazarenko, M. (2023). Genotype-mutagenic interaction in the cytogenetic variability of winter wheat for a new ecogenetic factor . Regulatory Mechanisms in Biosystems, 14(3), 370-377. https://doi.org/10.15421/10.15421/022355
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