Antagonistic properties of a probiotic preparation containing bacteria of the genera Bacillus and Enterococcus

  • O. M. Chechet State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • V. L. Kovalenko State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • O. I. Horbatyuk State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • O. S. Gaidei State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • O. L. Kravtsova State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • V. O. Andriyashchuk State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • I. V. Musiets State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
  • D. O. Ordynska State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination
Keywords: probiotic cultures; Bacillus subtilis; Bacillus licheniformis; Bacillus coagulans; Enterococcus faecium; symbiotic action


Complex probiotic preparations with spore-forming microorganisms of the genus Bacillus and other types of bacteria in their composition have a high biological activity and are of scientific and practical interest. In our laboratory experiments, we determined the level of antagonistic activity of the newly developed probiotic preparation containing strains of bacteria B. subtilis, B. licheniformis, B. coagulans in a complex with Enterococcus faecium, products of their own fermentation, other natural substances and a sorbent made of natural aluminosilicates. The antagonistic properties of the probiotic preparation were examined by diffusion methods of delayed antagonism and agar blocks. In order to identify the antagonistic action of the experimental probiotic preparation, we used Gram-negative and Gram-positive test cultures of bacteria from the Museum of Strains of Microorganism Cultures of the Research Bacteriology Department. The results of laboratory studies indicated the antagonistic effectiveness of the experimental probiotic preparation in its interaction with test microorganisms. This was confirmed by the indicators of the diameters of the growth inhibition zones of the tested bacteria, ranging 27–36 and more and corresponding to high and very high levels of antagonistic activity of the experimental probiotic. The use of the developed probiotic preparation in poultry farming would ensure the correct formation of microbiocenosis in gthe astrointestinal tract of poultry from hatching, increase local immunity, support a stable epizootic situation by preventing bacterial infections among poultry through biological means, and therefore contribute to production of ecologically high-quality and safe products of poultry farming.


Arena, M. P., Capozzi, V., Spano, G., & Fiocco, D. (2017). The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms. Applied Microbiology and Biotechnology, 101(7), 2641–2657.

Ashraf, R., & Shah, N. P. (2014). Immune system stimulation by probiotic icroorganisms. Critical Reviews in Food Science and Nutrition, 54(7), 938–956.

Balasubramanian, B., Li, T., & Kim, I. H. (2016). Effects of supplementing growing-finishing pig diets with Bacillus spp. probiotic on growth performance and meat-carcass grade qualitytraits. Revista Brasileira de Zootecnia, 45, 93–100.

Chukhrai, I. L. (2019). Probiotyky z pozytsiyi dokazovoyi medytsyny [Probiotics from the standpoint of evidence-based medicine]. Farmakolohichnyi chasopys, 3, 102–110 (in Ukrainian).

Dyshlyuk, N. V., & Orlova, A. V. (2017). Structure’s features of esophagus and it’s immune formations of quails. Scientific Messenger LNUVMBT named after S. Z. Gzhytskyj, 77, 3–6 (in Ukrainian).

Fadeenko, H. D., Hrydnev, A. E., & Dubrov, K. Y. (2018). Novye napravleniya v lechenii sindroma razdrazhennogo kishechnika: Aktsent na mikrobiom [New directions in the treatment of irritable bowel syndrome: Emphasis on microbiota]. Suchasna Hastroenterolohiia, 104, 56–61 (in Ukrainian).

Haldar, L., & Gandhi, D. N. (2016). Effect of oral administration of Bacillus coagulans B37 and Bacillus pumilus B9 strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model. Veterinary World, 9(7), 766–772.

Hedayati, M., & Manafi, M. (2018). Evaluation of anherbal compound, a commercial probiotic, and an antibiotic growth promoter on the performance, intestinal bacterial population, antibody titers, and morphology of the jejunum and ileum of broilers. Revista Brasileira de Ciência Avícola, 20(2), 305–316.

Hu, S., Mahfuz, S. U., Nahar, M. J., Mo, C., Ganf, Z., & Zhongju, L. (2017). Inclusion of probiotic on chicken performance and immunity: A review. International Journal of Poultry Science, 16(9), 328–335.

Hubska, O. Y., Kuzminets, A. A., & Koliada, O. K. (2019). Stan kyshkovoho mikrobiomu v patsiientiv z osteoartrytom [The state of the intestinal microbiome in patients with osteoarthritis]. Suchasna Hastroenterolohiia, 5, 18–25 (in Ukrainian).

Hudzenko, T. V., Konup, I. P., Voliuvach, O. V., Horshkova, O. H., Beliaieva, T. O., & Chaban, M. M. (2019). Vyluchennia fenolu z vody bakteriiamy Bacilus subtilis ONU551, adhezovanymy na nosiiakh riznoyi pryrody [Extraction of phenol from water by bacteria Bacilus subtilis ONU551 adhered to different types of noses]. Mikrobiolohiia i Biotekhnolohiia, 1, 36–47 (in Ukrainian).

Iegorov, В., Kananykhina, О., & Turpurova, Т. (2022). Probiotychni kormovi dobavky v hodivli silskohospodarskykh tvaryn [Probiotic feed additives in the years of agricultural animals]. Grain Products and Mixed Fodder’s, 21(4), 25–31 (in Ukrainian).

Ivchenko, V. M. (2004). Dovidnyk sanitarno-mikrobiolohichnykh metodiv doslidzhennia kharchovykh produktiv ta obiektiv dovkillia [Handbook of sanitary and microbiological methods of research of food products and environmental objects]. Bila Tserkva (in Ukrainian).

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Aktyvnist T- ta V-systemy klitynnoho imunitetu tvaryn v umovakh okysnoho stresu ta dii liposomalnoho preparatu [Activity of T- and B-systems of cellular immunity of animals in conditions of oxidative stress and action of liposomal preparation]. Ukrainian Journal of Ecology, 7(4), 536–541 (in Ukrainian).

Khyzhniak, O. S., & Krasnopolskyi, Y. M. (2012). Biotekhnolohicheskie aspekty sozdaniya preparatov na osnove probiotikov [Biotechnological aspects of creating preparations based on probiotics]. Vestnyk NTU KPI, Novye resheniya v sovremennykh tekhnolohiyakh, 44(950), 72–78 (in Ukrainian).

Kim, Y.-J., Park, J.-H., & Seo, K.-H. (2017). Comparison of the loads and antibiotic-resistance profiles of Enterococcus species from conventional and organic chicken carcasses in South Korea. Poultry Science, 97(1), 271–278.

Klaenhammer, T. R., Kleerebezem, M., Kopp, M. V., & Rescigno, M. (2012). The impact of probiotics and prebiotics on the immune system. Nature Reviews Immunology, 12, 728–734.

Krysenko, O. V., Skliar, T. V., Vinnikov, A. I., Slipetska, A. V., & Kudenko, S. S. (2010). Mikrobiolohichni aspekty probiotychnykh preparativ [Microbiological aspects of probiotic preparations]. Visnyk of Dnipropetrovsk University, Biology, Ecology, 18(2), 19–24 (in Ukrainian).

Kucheruk, M. D., Bilyk, R. I., & Ihnatovska, M. V. (2018). Eksperymentalne zastosuvannia probiotychnoho preparatu dlia orhanichnoho vyroshchuvannia kurei [Experimental use of a probiotic preparation for organic chicken farmin]. Theoretical and Applied Veterinary Medicine, 6(3), 12‒17 (in Ukrainian).

Kucheruk, M. D., Zasiekin, D. A., & Dymko, R. O. (2018). Mikrobiolohichne ta sanitarno-hihiienichne znachennia eubiozu kyshechnyka produktyvnykh tvaryn [Microbiological and sanitary-hygienic significance of intestinal eubiosis of productive animals]. Ukrainian Journal of Ecology, 8(2), 287–293 (in Ukrainian).

Lutgendorff, F., Nijmeijer, R. M., Sandström, P. A., Trulsson, L. M., Magnusson, K. E., Timmerman, H. M., van Minnen, L. P., Rijkers, G. T., Gooszen, H. G., & Akkermans, L. M. (2009). Probiotics prevent intestinal barrier dysfunction in acute pancreatitis in rats via induction of ileal mucosal glutathione biosynthesis. PLoS One, 4, 4512.

Macelline, W. H. D. S. P., Cho, H. M., Awanthika, H. K. T., Wickramasuriya, S. S., Jayasena, D. D., Tharangani, R. M. H., Song, Z., & Heo, J. M. (2017). Determination of the growth performances and meat quality of broilers fed saccharomyces cerevisiaeas a probiotic in two different feeding intervals. Korean Journal of Poultry Science, 44(3), 161–172.

Mingmongkolchai, S., & Panbangre, W. (2018). Bacillus probiotics: An alternative to antibiotics for livestock production. Journal of Applied Microbiology, 124(6), 1334–1346.

Mousavi, S. M. A. A., Hosseini, H. M., & Mirhosseini, S. A. (2018). A review of dietary probiotics in poultry. Journal of Applied Biotechnology Reports, 5(2), 48–54.

Romanovych, M. M. (2018). Dynamika humoralnykh faktoriv zakhystu u kurchat-broileriv za umov zastosuvannia probiotychnykh preparativ [Dynamics of humoral protective factors in broiler chickens under the conditions of probiotic preparations]. Naukovyi Visnyk LNUVMB imeni S. Z. Gzhytskoho, 20(83), 264–267 (in Ukrainian).

Shkromada, O. I., & Dudchenko, Y. A. (2021). Doslidzhennia antymikrobnoyi aktyvnosti probiotychnykh shtamiv Bacillus [Study of the antimicrobial activity of probiotic strains of Bacillus]. Visnyk Sumskoho Natsionalnoho Ahrarnoho Universytetu, Seriia Veterynarna Medytsyna, 55, 38–43 (in Ukrainian).

Shkromada, O. I., Fotina, T. I., Fotina, H. A., Nechyporenko, O. L., Petrov, R. V., & Fotin, A. I. (2022). Vplyv Bacillus subtilis na porosiat na vidluchenni [Effects of Bacillus subtilis on piglets at weaning]. Visnyk Sumskoho Natsionalnoho Ahrarnoho Universytetu, Seriia Veterynarna Medytsyna, 56, 51–57 (in Ukrainian).

Skrypnyk, I. M., Kryvoruchko, I. H., Hopko, O. F., & Prykhodko, N. P. (2020). Suchasni mozhlyvosti korektsiyi vistseralnoyi hiperchutlyvosti u khvorykh na syndrom razdraznenogo kishechnika [Modern possibilities of correction of visceral hypersensitivity in patients with irritable bowel syndrome]. Suchasna Hastroenterolohiia, 2, 37–44 (in Ukrainian).

Stoianovskyi, V. H., Kolomiiets, I. A., Harmata, L. S., & Kamratska, O. I. (2018). Zminy morfofunktsionalnoho stanu orhaniv endokrynnoji ta imunnoji system perepeliv promyslovoho vyroshchuvannia za diji stresu [Changes in the morphofunctional state of the endocrine and immune systems of quails in industrial cultivation under stress]. Fiziolohichnyi Zhurnal, 64(1), 25–33 (in Ukrainian).

Sychevskyi, M. L., & Danylenko, S. H. (2016). Doslidzhennia vplyvu funktsionalnoyi dobavky Bk-ptytsia na fizyko-khimichni pokaznyky miazovoyi tkanyny kurchat-broileriv [Study of the effect of the functional additive Bk-bird on the physicochemical parameters of the muscle tissue of broiler chickens]. Tekhnologii Pyshchevoy, lehkoy i khimicheskoy promyshlennosti, 30, 56–60 (in Ukrainian).

Tang, X., Liu, X., & Liu, H. (2021). Effects of dietary probiotic (Bacillus subtilis) supplementation on carcass traits, meat quality, amino acid, and fatty acid profile of broiler chickens. Frontiers in Veterinary Specience, 8, 767–802.

Tochylyna, A. H., Belova, Y. V., & Soloveva, Y. V. (2014). Izuchenie biolohicheskikh svoistv shtammov roda Lactobacillus [Study of biological properties of strains of the genus Lactobacillus]. Sovremennye Problemy Nauky i Obrazovaniya, 12, 45–51 (in Ukrainian).

Trufanov, O. V., Kotyk, A. M., & Bozhok, L. V. (2008). Efektyvnist’ probiotychnoho preparatu na osnovi Bacillus subtilis (BPS-44) pry eksperymentalnykh mikotoksykozakh kurchat [Effectiveness of a probiotic preparation based on Bacillus subtilis (BPS-44) in experimental mycotoxicosis of chickens]. Mikrobiolohichnyi Zhurnal, 70(1), 52–58 (in Ukrainian).

Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., Zazharska, N. M., & Brygadyrenko, V. V. (2020). Antibacterial and fungicidal activities of ethanol extracts of 38 species of plants. Biosystems Diversity, 28(3), 281–289.

How to Cite
Chechet, O. M., Kovalenko, V. L., Horbatyuk, O. I., Gaidei, O. S., Kravtsova, O. L., Andriyashchuk, V. O., Musiets, I. V., & Ordynska, D. O. (2022). Antagonistic properties of a probiotic preparation containing bacteria of the genera Bacillus and Enterococcus . Regulatory Mechanisms in Biosystems, 13(4), 362-366.