Physicochemical quality indicators of honey: An evaluation in a Ukrainian socioecological gradient

  • A. Zhuk Yuriy Fedkovych Chernivtsi National University
  • I. Sytnikova Yuriy Fedkovych Chernivtsi National University
  • T. Fylypchuk Yuriy Fedkovych Chernivtsi National University
  • O. Bahlei Yuriy Fedkovych Chernivtsi National University
  • O. Shkrobanets Yuriy Fedkovych Chernivtsi National University
  • J. Danihlík Palacký University Olomouc
  • H. Moskalyk Yuriy Fedkovych Chernivtsi National University
  • I. Panchuk Yuriy Fedkovych Chernivtsi National University
  • V. Burkut Yuriy Fedkovych Chernivtsi National University
  • P. Angelstam Inland Norway University of Applied Sciences
  • M. Fedoriak Yuriy Fedkovych Chernivtsi National University
Keywords: polyfloral honey; private apiaries; honey quality; honey standards; Chernivtsi region; Ukraine


Physical and chemical quality indicators of 65 polyfloral honey samples from three administrative districts of Chernivtsi region in South Western Ukraine were studied. The chosen administrative districts were Putyla (representing the ‘Traditional villages’ stratum), Storozhynets (the ‘Intermediate’ stratum) and Khotyn (the ‘Intensive agriculture’ stratum), which reflect a steep gradient of social and ecological conditions such as land cover and land use, level of economic development, culture and demography. The quality of honey was determined in accordance with the requirements of the Ukrainian national standard and the EU Directive relating to honey (or Codex Alimentarius Honey Standard) by using the following indicators: reducing sugars and moisture content, diastase activity, free acidity, pH, electrical conductivity, hydroxymethylfurfural (HMF) and proline content. The profile of carbohydrates was analyzed, in particular glucose, fructose, sucrose, maltose, trehalose, melezitose, raffinose. The ratio of fructose to glucose (F/G) was determined. All tested samples complied with the international standards for the mass fraction of reducing sugars, diastase activity and hydroxymethylfurfural content. For physical and chemical parameters, the studied honey samples were of high quality. The content of the reducing sugars in the honey samples varied within the range of 66.0–97.6%. The fructose content ranged from 342 to 549 mg/g, and the glucose content variation ranged within 283–517 mg/g. The average fructose/glucose ratio was 1.2 for honey samples from the three studied districts. Besides fructose and glucose, some oligosaccharides, such as maltose, trehalose and melezitose, were found in the examined honey. Melezitose was detected in the honey samples from ‘Traditional village’ (21 samples) and ‘Intermediate’ (5 samples) districts. The total variability of HMF content in the studied 65 honey samples from apiaries in the three districts of Chernivtsi region ranged from 0.19 to 30.8 mg/kg. The minimum moisture content was found to be 16.2% (in the ‘Traditional village’ and ‘Intermediate’ strata), and 22.2% was the maximum (in the ‘Intermediate’ stratum). Our studies have shown that free acidity of the samples varied within the range 13.5 to 58.0 meq/kg. Proline content variability for the three studied geographical areas ranged from 82.3 to 1201.2 mg/kg. The studied samples of honey had a low pH level (~ 3.7), high content of proline (~ 513 mg/kg) and reducing sugars (~ 80%), which indicates its nutritional value and naturalness. Deviations from the honey standards in moisture content, acidity and electrical conductivity was revealed in 8% to 10% of all samples.


Acquarone, C., Buera, P., & Elizalde, B. (2007). Pattern of pH and electrical conductivity upon honey dilution as a complementary tool for discriminating geographical origin of honeys. Food Chemistry, 101(2), 695–703.

Adamchuk, L., Sukhenko, V., & Akulonok, O. (2019). Fermentatyvna aktyvnist akatsiievoho medu [Enzymatic activity of black locust honey]. Emerging Technologies, 10, 52–61 (in Ukrainian).

Al-Hatamleh, M. A., Hatmal, M. M. M., Sattar, K., Ahmad, S., Mustafa, M. Z., Bittencourt, M. D. C., & Mohamud, R. (2020). Antiviral and immunomodulatory effects of phytochemicals from honey against COVID-19: Potential mechanisms of action and future directions. Molecules, 25(21), 5017.

Alias, N., Ching, K. S., Ab Karim, A. N., Al-Rahmi, W. M., & Al-Maatouk, Q. (2018). Numerical performance of healthy processing for HMF content in honey. International Journal of Engineering and Technology, 7(4), 3687–3689.

Bálint, M., Ujvárosi, L., Theissinger, K., Lehrian, S., Mészáros, N., & Pauls, S. U. (2011). The Carpathians as a major diversity hotspot in Europe. In: Zachos, F. E., & Habel, J. C. (Eds.). Biodiversity hotspots. Springer, Berlin, Heidelberg. Pp. 189–205.

Bogdanov, S., & Gfeller, M. (2006). Classification of honeydew and blossom honeys by discriminant analysis. Agroscope Liebefeld Posieux, 500, 3–7.

Bogdanov, S., Jurendic, T., Sieber, R., & Gallmann, P. (2008). Honey for nutrition and health: A review. Journal of the American college of Nutrition, 27(6), 677–689.

Bogdanov, S., Lüllmann, C., Martin, P., von der Ohe, W., Russmann, H., Vorwohl, G., Oddo, L. P., Anna-Gloria Sabatini, A.-G., Marcazzan, G. L., Piro, R., Flamini, C. C., Morlot, M., Lhéritier, J., Borneck, R., Marioleas, P., Tsigouri, A., Kerkvliet, J., Ortiz, A., Ivanov, T., D’Arcy, B., Mossel, B., & Vit, P. (1999). Honey quality and international regulatory standards: Review by the International Honey Commission. Bee World, 80(2), 61–69.

Cervantes, M. R., Novelo, S. G., & Duch, E. S. (2000). Effect of the temporary thermic treatment of honey on variation of the quality of the same during storage. Apiacta, 35(4), 162–170.

Cherevatov, O. V., Melnik, E. O., & Volkov, R. A. (2020). Polimorfizm hena СоI u medonosnykh bdzhil z riznykh rehioniv Ukrainy [Polymorphism of CоI gene in honey bees from different regions of Ukraine]. The Bulletin of Vavilov Society of Geneticists and Breeders of Ukraine, 18, 22–28 (in Ukrainian).

Cherevatov, O. V., Panchuk, I. I., Kerek, S. S., & Volkov, R. A. (2019). Molekuliarne riznomanittia speisernoi dilianky СoI-СoII mitokhondrialnoho henomu ta pokhodzhennia Karpatskoi bdzholy [Molecular diversity of the CoI–CoII spacer region in the mitochondrial genome and the origin of the arpathian bee]. Cytology and Genetics, 53(4), 276–281 (in Ukrainian).

Codex Alimentarius Commission (2001). Revised Codex Standard for Honey Codex Stan 12-1981. Rev. 2. Codex Standard, 12, 1–7.

Cozmuta, A. M., Cozmuta, L. M., Varga, C., Marian, M., & Peter, A. (2011). Effect of thermal processing on quality of polyfloral honey. Romanian Journal of Food Science, 1(1), 45–52.

da Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: Chemical composition, stability and authenticity. Food Chemistry, 196, 309–323.

Dankevych, V., Dankevych, Y., & Pyvovar, P. (2018). Formuvannia koniunktury na svitovomu rynku medu: Suchasnyi stan ta perspektyvy ukrainskykh eksporteriv [Forming conjuncture in the world honey market: Current state and prospects for Ukrainian exporters]. Agricultural and Resource Economics, 4(2), 37–54 (in Ukrainian).

Fedoriak, M. M., Angelstam, P. K., Kulmanov, O. L., Tymochko, L. I., Rudenko, S. S., & Volkov, R. A. (2019). Ukraine is moving forward from ‘Undiscovered honey land’ to active participation in international monitoring of honey bee colony losses. Bee World, 96(2), 50–54.

Fedoriak, M., Kulmanov, O., Zhuk, A., Shkrobanets, O., Tymchuk, K., Moskalyk, G., Olendr, T., Yamelynets, T., & Angelstam, P. (2021). Stakeholders’ views on sustaining honey bee health and beekeeping: The roles of ecological and social system drivers. Landscape Ecology, 36(3), 763–783.

Gismondi, A., De Rossi, S., Canuti, L., Novelli, S., Di Marco, G., Fattorini, L., & Canini, A. (2018). From Robinia pseudoacacia L. nectar to Acacia monofloral honey: Biochemical changes and variation of biological properties. Journal of the Science of Food and Agriculture, 98, 4312–4322.

Halouzka, R., Tarkowski, P., & Cavar, Z. S. (2016). Characterisation of phenolics and other quality parameters of different types of honey. Czech Journal of Food Sciences, 34, 244–253.

Iqbal, A., Kamran, H., Khalid, S., Jabeen, S., & Aslam, M. (2020). Glycemic response of natural sweeteners like sugarcane juice, honey and jaggery in healthy individuals. EAS Journal of Humanities and Cultural Studies, 2(5), 38–41.

Isopescu, R. D., Josceanu, A. M., Minca, I., Colta, T., Postelnicescu, P., & Mateescu, C. (2014). Characterization of Romanian honey based on physicochemical properties and multivariate analysis. Revista de Chimie-Bucharest, 65, 381–385.

Karabagias, I. K., Badeka, A., Kontakos, S., Karabournioti, S., & Kontominas, M. G. (2014). Characterisation and classification of Greek pine honeys according to their geographical origin based on volatiles, physicochemical parameters and chemometrics. Food Сhemistry, 146, 548–557.

Kowalski, S., Łukasiewicz, M., & Berski, W. (2013). Applicability of physico-chemical parameters of honey for identification of the botanical origin. Acta Scientiarum Polonorum Technologia Alimentaria, 12(1), 51–59.

Lazareva, L. M. (2015). Pokaznyk vmistu prolinu yak kryterii otsinky yakosti medu riznoho botanichnoho pokhodzhennia [Indicator of proline content as a criterion for assessing the quality of honey different botanical origin]. Scientific and Technical Bulletin of the Research Center for Biosafety and Environmental Control of Agricultural Resources, 3(4), 97–101 (in Ukrainian).

Lazareva, L. M., & Postoienko, V. O. (2016). Yakist ta bezpechnist meda z soniashnyka [Quality and safety of honey with sunflower]. Scientific and Technical Bulletin of the Research Center for Biosafety and Environmental Control of Agricultural Resources, 4(2), 95–101 (in Ukrainian).

Nachychko, V., & Honcharenko, V. (2017). Botaniko-heohrafichna kharakterystyka vydiv rodu Thymus L. (Lamiaceae) u flori zakhidnykh rehioniv Ukrainy [Botanical-geographical characterization of Thymus L. (Lamiaceae) species in the flora of western regions of Ukraine]. Visnyk of the Lviv University, Biological series, 75, 35–47 (in Ukrainian).

Nousias, P., Karabagias, I. K., Kontakos, S., & Riganakos, K. A. (2017). Characterization and differentiation of Greek commercial thyme honeys according to geographical origin based on quality and some bioactivity parameters using chemometrics. Journal of Food Processing and Preservation, 41(4), e13061.

Partelow, S. (2018). A review of the social-ecological systems framework: Applications, methods, modifications, and challenges. Ecology and Society, 23(4), 36.

Pavlova, T., Stamatovska, V., Kalevska, T., Dimov, I., & Nakov, G. (2018). Quality characteristics of honey: A review. Proceedings of University of Ruse, 57, 32–37.

Postoienko, V. O., Lazareva, L. M., & Yaremchuk, O. S. (2019). Оsnovni pokaznyky otsinky yakosti i bezpechnosti medu bdzholynoho v Ukraini ta yikh harmonizatsiia z vymohamy ES [The main indicators to measure the quality and safety of honey bee in Ukraine and their harmonization with EU requirements]. East European Scientific Journal, 52, 14–21 (in Ukrainian).

Prazina, N., & Mahmutović, O. (2017). Analysis of biochemical composition of honey samples from Bosnia and Herzegovina. International Journal of Research in Applied, Natural and Social Sciences, 5(3), 73–78.

Primorac, L., Angelkov, B., Mandić, M. L., Kenjerić, D., Nedeljko, M., Flanjak, I., & Arapceska, M. (2009). Comparison of the Croatian and Macedonian honeydew honey. Journal of Central European Agriculture, 10(3), 263–264.

Ratiu, I. A., Al-Suod, H., Bukowska, M., Ligor, M., & Buszewski, B. (2020). Correlation study of honey regarding their physicochemical properties and sugars and cyclitols content. Molecules, 25(1), 34.

Rybak-Chmielewska, H., Szczęsna, T., Waś, E., Jaśkiewicz, K., & Teper, D. (2013). Characteristics of Polish unifloral honeys IV. Honeydew honey, mainly Abies alba L. Journal of Apicultural Science, 57(1), 51–59.

Shapla, U. M., Solayman, M., Alam, N., Khalil, M. I., & Gan, S. H. (2018). 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: Effects on bees and human health. Chemistry Central Journal, 12(1), 1–18.

Thrasyvoulou, A., Tananaki, C., Goras, G., Karazafiris, E., Dimou, M., Liolios, V., Kanelis, D., & Gounari, S. (2018). Legislation of honey criteria and standards. Journal of Apicultural Research, 57(1), 88–96.

Yücel, Y., & Sultanog, P. (2013). Characterization of honeys from Hatay Region by their physicochemical properties combined with chemometrics. Food Bioscience, 1, 16–25.

How to Cite
Zhuk, A., Sytnikova, I., Fylypchuk, T., Bahlei, O., Shkrobanets, O., Danihlík, J., Moskalyk, H., Panchuk, I., Burkut, V., Angelstam, P., & Fedoriak, M. (2022). Physicochemical quality indicators of honey: An evaluation in a Ukrainian socioecological gradient . Regulatory Mechanisms in Biosystems, 13(4), 354-361.