Efficacy of hepatoprotectors in prophylaxis of hepatosis of laying hens

  • L. G. Slivinska Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • V. Y. Yaremchuk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • A. R. Shcherbatyy Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • H. O. Zinko Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
Keywords: metabolism; liver; blood serum; productivity; poultry survival; enzymes; proteins.


Hepatoses of laying hens are quite common in poultry farms as a result of improper practices of poultry maintenance consisting in excessive number of protein feeds for provision of high productivity. The objective of the study was preventive efficacy of Gep-A-Stress hepatoprotectors (carnitine hydrochloride, D L methionine, sorbitol, choline chloride, magnesium sulfate heptahydrate) and Hepasan VS (L-carnitine hydrochloride, sorbitol, choline chloride, magnesium sulfate hepahydrate, betaine hydrochloride, L-arginin) against hepatosis of laying hens. To determine the efficacy of Hep-A-Stres and Hepasan-VS hepatoprotectors during production tests (n = 4,500), we monitored the parameters of survival rate (the final number of individuals as percentage of the initial number) and egg productivity of laying hens. We determined that after 30 days of using the hepatoprotectors, the content of overall protein in blood serum of laying hens of the first and the second experimental groups decreased by 21.4% and 18.9% compared with the parameters prior to providing the hepatoprotectors and by 26.3% and 23.3% compared with the control group after receiving the drug. The urea contents in blood serum increased by 19.0% and 10.5%. Compared with the control, the activity of alanine aminotransferase decreased by 43.7% and 24.1% in the first experimental group and by 23.4% and 14.9% in the second. The activity of aspartate aminotransferase in blood serum of the experimental groups decreased by 10.7%. The cholesterol concentration decreased by 50% and 58.3%. The content of triaglycerols decreased by 24.1% and 8.9% respectively. The concentrations of high-density lipoproteins after 30 days of the experiment decreased by 33.3% and 77.8% respectively, the content of low-density lipoproteins decreased by 61.3% and 40.4% and 42.3%. Population maintenance equaled respectively 97.1%, 98.3% and 98.1%. At the end of the experiment, the egg productivity of laying hens of the first and second groups increased by 4% and 3.6% compared with the control. Therefore, intake of the hepaprotectors by laying hens stimulated their metabolism, positively influenced the blood parameters, survival and egg productivity. The conducted studies confirm the benefits of using hepatoprotectors for the prohylaxis of hepatosis of hens.


Avdosjeva, I. K., Regenchuk, V. V., & Melnychuk, I. L. (2013). Gepatoprotektory dlja ptahivnyctva [Hepatoprotectors for poultry]. Tvarynnyctvo Sogodni, 7, 62–65 (in Ukrainian).
Avdosjeva, I. K., Temnenko, S. M., Kalynovska, L. V., & Zdolyny, S. O. (2014). Efektyvnist’ gepatoprotektora Gepabial’karnityn pry vyroshhuvanni brojleriv [Efficacy of hepatoprotector hepabialcarnitine in broiler farming]. Naukovyj Visnyk L’vivs'kogo Nacional’nogo Universytetu Veterynarnoji Medycyny ta Biotehnologij Imeni S. Z. Gzhyckogo, 16(60), 3–11 (in Ukrainian).
Bashchenko, M. I., Boiko, О. V., Honchar, О. F., Gutyj, B. V., Lesyk, Y. V., Ostapyuk, A. Y., Kovalchuk, І. І., & Leskiv, K. Y. (2020). The effect of milk thistle, metiphen, and silimevit on the protein-synthesizing function of the liver of laying hens in experimental chronic cadmium toxicosis. Ukrainian Journal of Ecology, 10(6), 164–168.
Brezvyn, O. M., Guta, Z. A., Gutyj, B. V., Fijalovych, L. M., Karpovskyi, V. I., Shnaider, V. L., Farionik, T. V., Dankovych, R. S., Lisovska, T. O., Bushuieva, I. V., Parchenko, V. V., Magrelo, N. V., Slobodjuk, N. M., Demus, N. V., Leskiv, K. Y. (2021). The influence of HamekoTox on the morphological and biochemical indices of the blood of laying hens in spontaneous fumonisin toxicosis. Ukrainian Journal of Ecology, 11(2), 249–253.
Chowdhury, V. S., Ouchi, Y., Haraguchi, S., & Bungo, T. (2021). Liver metabolomic analysis in broiler chicks: Profiling the metabolites after oral administration of l-citrulline. Animal Science Journal, 92(1), e13609.
Dhawale, A. (2007). The liver: A big organ with a big role. World Poultry, 23(10), 34–36.
Dong, X. F., Zhai, Q. H., Tong, J. M. (2021). Dietary choline supplementation regulated lipid profiles of egg yolk, blood, and liver and improved hepatic redox status in laying hens. Poultry Science, 98(8), 3304–3312.
El-Katcha, M. I., Soltan, M. A., El-Naggar, K., El-Shobokshy, S. A., & El-Erian, M. A. (2019). Laying performance, fat digestibility and liver condition of laying hens supplemented with vitamin B12 or Biotin and/or bile acids in diet. Slovenian Veterinary Research, 56(22), 341–352.
Gao, S. T., Ma, L., Zhang, Y. D., Wang, J. Q., Loor, J. J., & Bu, D. P. (2021). Hepatic transcriptome perturbations in dairy cows fed different forage resources. BMC Genomics, 22(1), 35.
Gu, W., Wen, K., Yan, C., Li, S., Liu, T., Xu, C., Liu, L., Zhao, M., Zhang, J., Geng, T., & Gong, D. (2020). Maintaining intestinal structural integrity is a potential protective mechanism against inflammation in goose fatty liver. Poultry Science, 99(11), 5297–5307.
Gu, Y. F., Chen, Y. P., Jin, R., Wang, C., Wen, C., & Zhou, Y. M. (2021). Age-related changes in liver metabolism and antioxidant capacity of laying hens. Poultry Science, 100(12), 101478.
Gutyj, B. V., Ostapyuk, A. Y., Sobolev, O. I., Vishchur, V. J., Gubash, O. P., Kurtyak, B. M., Kovalskyi, Y. V., Darmohray, L. M., Hunchak, A. V., Tsisaryk, O. Y., Shcherbatyy, A. R., Farionik, T. V., Savchuk, L. B., Palyadichuk, O. R., & Hrymak, K. (2019). Cadmium burden impact on morphological and biochemical blood indicators of poultry. Ukrainian Journal of Ecology, 9(1), 235–239.
Jarosz, L., Marek, A., Gradzki, Z., Laskowska, E., & Kwiecien, M. (2019). Effect of zinc sulfate and zinc glycine chelate on concentrations of acute phase proteins in chicken serum and liver tissue. Biological Trace Element Research, 187(1), 258–272.
Kaneko, J. J., Harvey, W., & Bruss, M. L. (1997). Clinical biochemistry of domestic animals. Academmic Press, New York.
Karpenko, Y., Hunchak, Y., Gutyj, B., Hunchak, A., Parchenko, M., & Parchenko, V. (2022). Advanced research for physico-chemical properties and parameters of toxicity piperazinium 2-((5-(furan-2-YL)-4-phenyl-4H-1,2,4-triazol-3-yl)thio)acetate. ScienceRise: Pharmaceutical Science, 2(36), 18–25.
Laptjeva, K. A. (2012). Funkcional'nyj stan pechinky kurej-nesuchok pid vplyvom pljumbumu acetatu za alimentarnogo hronichnogo toksykozu [Functional state of the liver of laying hens under the influence of lead acetate in alimentary chronic toxicosis]. Biologija Tvaryn, 14, 321–327 (in Ukrainian).
Levchenko, V. I., Mel'nyk, A. J., & Moskalenko, V. P. (2017). Vplyv preparatu Gep-A-stres na obmin rechovyn u kurchat-brojleriv [The effect of Hep-A-stress on metabolic exchange in chicken broilers]. Naukovyj Visnyk Veterynarnoji Medycyny, 133, 48–55 (in Ukrainian).
Liu, T., Xing, Y., Fan, X., Chen, Z., Zhao, C., Liu, L., Zhao, M., Hu, X., Dong, B., Wang, J., Cui, H., Gong, D., & Geng, T. (2021). Fasting and overfeeding affect the expression of the immunity- or inflammation-related genes in the liver of poultry via endogenous retrovirus. Poultry Science, 100(2), 973–981.
Melnyk, A. J. (2017). Dejaki pokaznyky bilkovo-lipidnogo obminu ta funkcional’nyj stan pechinky v kurchat-brojleriv za vykorystannja preparatu “Abetka dlja tvaryn” [Some indicators of protein-lipid metabolism and functional status of the liver in broiler chickens with the use of the drug “Alphabet for animals”]. Naukovyj Visnyk Veterynarnoji Medycyny, 136, 69–78 (in Ukrainian).
Milroy, T. H. (1993). The formation of uric acid in birds. The Journal of Physiology, 30(1), 47–60.
Mylostyvyi, R., Lesnovskay, O., Karlova, L., Khmeleva, O., Кalinichenko, O., Orishchuk, O., Tsap, S., Begma, N., Cherniy, N., Gutyj, B., & Izhboldina, O. (2021). Brown Swiss cows are more heat resistant than Holstein cows under hot summer conditions of the continental climate of Ukraine. Journal of Animal Behaviour and Biometeorology, 9(4), 2134.
Mylostyvyi, R., Sejian, V., Izhboldina, O., Kalinichenko, O., Karlova, L., Lesnovskay, O., Begma, N., Marenkov, O., Lykhach, V., Midyk, S., Cherniy, N., Gutyj, B., & Hoffmann, G. (2021). Changes in the spectrum of free fatty acids in blood serum of dairy cows during a prolonged summer heat wave. Animals, 11(12), 3391.
Nishchemenko, M. P., Samorai, M. M., Prokopishyna, T. B., Poroshynska, O. A., & Stovbetska, L. S. (2013). Vplyv zghodovuvannia mikormu kurkam-nesuchkam na aktyvnist luzhnoi fosfatazy ta obmin kaltsiiu i neorhanichnoho fosforu v yikh orhanizmi [The effect of feeding micronutrients to laying hens on the activity of alkaline phosphatase and metabolism of calcium and inorganic phosphorus in their body]. Visnyk Sumskoho Natsionalnoho Ahrarnoho Universytetu, Seriia Veterynarna Medytsyna, 2(32), 3–6 (in Ukrainian).
Ostapyuk, A. Y., Holubieva, T. A., Gutyj, B. V., & Slobodian, S. O. (2021). The effect of sylimevit, metifen, and milk thistle on the intensity of the processes of peroxidation of lipids in the body of laying hens in experimental chronic cadmium toxicosis. Ukrainian Journal of Ecology, 11(4), 57–63.
Pu, S., Usuda, K., Nagaoka, K., Gore, A., Crews, D., & Watanabe, G. (2020). The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature. Poultry Science, 99(9), 4586–4597.
Qureshi, I. A., Khan, S. A., Chaudhry, Z. I., Mian, N. A., Tipu, M. Y., & Rai, M. F. (2004). Еffects of high dietary fat on serum cholesterol and fatty liver syndrome in broilers. Pakistan Veterinary Journal, 24(3), 153–154.
Shcherbatyy, A., & Slivinska, L. (2021). Overview: Prevalence and structure of metabolic diseases of laying chickens, their influence on egg quality and condition of young chickens. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, Series Veterinary Sciences, 23(104), 3–9.
Shini, A., Shini, S., & Bryden, W. L. (2019). Fatty liver haemorrhagic syndrome occurrence in laying hens: Impact of production system. Avian Pathology, 48(1), 25–34.
Sobolev, O., Gutyj, B., Petryshak, R., Pivtorak, J., Kovalskyi, Y., Naumyuk, A., Petryshak, O., Semchuk, I., Mateusz, V., Shcherbatyy, A., & Semeniv, B. (2019). Biological role of selenium in the organism of animals and humans. Ukrainian Journal of Ecology, 8(1), 654–665.
Surai, P. F., Kochish, I. I., Fisinin, V. I., & Velichko, O. A. (2018). Selenium in poultry nutrition: From sodium selenite to organic selenium sources (review). The Journal of Poultry Science, 55(2), 79–93.
Tao, C., Zhang, B. Y., Wei, X. T., Zhao, M., Sun, Z. J., Wang, S., Bi, J. W., Qi, D. S., Sun, L. H., & Zhang, N. Y. (2020). Effects of dietary cadmium supplementation on production performance, cadmium residue in eggs, and hepatic damage in laying hens. Environmental Science and Pollution Research, 27(26), 33103–33111.
Tilbrook, A. J., & Fisher, A. D. (2020). Stress, health and the welfare of laying hens. Animal Production Science, 61(10), 931–943.
Underwood, G., Andrews, D., & Phung, T. (2021). Advances in genetic selection and breeder practice improve commercial layer hen welfare. Animal Production Science, 61(10), 856–866.
Vasylyev, D., Priimenko, B., Aleksandrova, K., Mykhalchenko, Y., Gutyj, B., Mazur, I., Magrelo, N., Sus, H., Dashkovskyy, O., Vus, U., & Kamratska, O. (2021). Investigation of the acute toxicity of new xanthine xenobiotics with noticeable antioxidant activity. Ukrainian Journal of Ecology, 11(1), 315–318.
Xu, E., Zhang, L., Yang, H., Shen, L., Feng, Y., Ren, M., & Xiao, Y. (2019). Transcriptome profiling of the liver among the prenatal and postnatal stages in chickens. Poultry Science, 98(12), 7030–7040.
Yan, F., Murugesan, G., & Cheng, H. (2019). Effects of probiotic supplementation on performance traits, bone mineralization, cecal microbial composition, cytokines and corticosterone in laying hens. Animal, 13, 33–41.
Yaremchuk, V. Y., & Slivinska, L. G. (2020). Prevention of hepatosis in laying hens using hepatoprotectors Hep-A-Stress and Hepasan-VS. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(3), 8–14.
Zaefarian, F., Abdollahi, M. R., Cowieson, A., & Ravindran, V. (2019). Avian liver: the forgotten organ. Animals, 9(2), 63.
Zhao, M., Xing, Y., Liu, L., Fan, X., Liu, L., Geng, T., & Gong, D. (2020). GC-TOF-MS-Based metabolomics analyses of liver and intestinal contents in the overfed vs. normally-fed geese. Animals, 10(12), 2375.
How to Cite
Slivinska, L. G., Yaremchuk, V. Y., Shcherbatyy, A. R., Gutyj, B. V., & Zinko, H. O. (2022). Efficacy of hepatoprotectors in prophylaxis of hepatosis of laying hens . Regulatory Mechanisms in Biosystems, 13(3), 287-293. Retrieved from https://medicine.dp.ua/index.php/med/article/view/825

Most read articles by the same author(s)

> >>