Biological activity of soybean seed lectin at the spraying of Glycine max plants against the background of seed treatment with pesticide containing fipronil, thiophanate-methyl, pyraclostrobin as active substances and rhizobial bacterization

  • O. V. Kyrychenko Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine
  • S. Y. Kots Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine
  • A. V. Khrapova Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine
  • S. V. Omelchuk Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine
Keywords: soybean-rhizobial symbiosis; Bradyrhizobium japonicum 634b; fungicide; lectin; nitrogenase activity; photosynthetic pigments; grain productivity

Abstract

Preparations for protecting plants, particularly those with fungicidal activity, continue to be relevant in agricultural production. They are used to effectively combat phytopathogens and ensure high yield of cultivated plants. However, they are among the anthropogenic factors which impose a heavy chemical load on ecosystems. Data about the effects of pesticides on physiological parameters of plants are essential for understanding the main regulatory mechanisms as preconditions to the phytotoxic state of compounds, as well as greater substantial understanding of the functional condition and implementation of adaptive potential of plants during and post stress. An important and relevant task – both practically and theoretically in the conditions of action of fungicide on seeds - is studying the possibilities of application of phytolectines as biologically active compounds with broad spectrum of action, including fungicidal effect, for spraying legumes in order to stabilize their development, ensure effective functioning of legume-rhizobial symbioses and cause fuller realization of productive potential against the background of decrease in chemical pressure on agrocenoses. Therefore, we aimed to evaluate the biological activity of soybean seed lectin (according to the parameters of productivity and functional activity of soybean-rhizobial symbiosis) at the spraying of Glycine max (L.) Merr. plants against the background of seed treatment of pesticide, Standak Top with fungicidal and insecticidal actions on the day of sowing and inoculation with Bradyrhizobium japonicum 634b. We used physiological, biochemical, microbiological and statistical methods of studies. We determined that Standak Top, applied on soybeans that were afterwards inoculated with rhizobia, exerted negative tendency on formation of vegetative mass by plants at the beginning of vegetation, though in the following phases of ontogenesis, their development and productivity reached the level of the control. Nitrogenase activity of symbiosis and the condition of photosynthetic pigment complex (content of chlorophyll and carotenoids and their ratio) were at the level or significantly lower than in the control plants. After spraying soybeans with lectin (without use of the fungicide) in the phase of development of two true leaves, there occurred significant increase in functional activity of the symbiotic system (according to total nitrogenase activity of symbiosis, higher by 1.91 and 1.79 times compared with the controls with inoculation and inoculation + fungicide) and the content of photosynthetic pigments (chlorophylls were higher by 1.12–1.45 times, carotenoids by 1.14–1.39 times) and development of strong leaf apparatus (by 1.33–1.42 times). This caused highest level of realization (by 13.9% and 10.1% higher compared with the controls with inoculation and inoculation + fungicide) of productive potential of cultivated plants. After spraying plants with soybean lectin against the background of use of fungicide, notable and reliable increases occurred in the level of absorption of molecular nitrogen (by 1.72 and 1.52 times according to total activity of symbiosis, compared with the controls with inoculation and inoculation + fungicide), content of chlorophyll (1.25–1.64 times) and carotenoids (1.12–1.42 times) in leaves of soybean, and also plants were actively developing during vegetation (1.12–1.40 times), producing yield that exceeded by 12.8% and 9.1% the controls with inoculation and inoculation + fungicide. Therefore, use of soybean seed lectin for spraying plants against the background of seed treatment of pesticide Standak Top on day of sowing can stabilize and even increase the level of realization of symbiotic and productive potential of soybean-rhizobial symbiosis compared both with the control (inoculation with rhizobia) and the variant with treatment of seeds (rhizobia + fungicide). This indicates on the perspectives of further studies of biological activity of phytolectins aiming at decreasing chemical pressure on ecosystems by leveling out or decreasing the negative impact of chemical means of protection on the plants and symbiosis.

References

Ahmed, A. M., Heikal, M. D., & Hindawy, O. S. (1983). Side effects of benomyl (fungicide) treatments on sunflower, cotton and cowpea plants. Phyton, 23(2), 185–195.

Araujo, R. S., Cruz, S. P. D., Souchie, E. L., Martin, T. N., Nakatani, A. S., Nogueira, M. A., & Hungria, M. (2017). Preinoculation of soybean seeds treated with agrichemicals up to 30 days before sowing: Technological innovation for large-scale agriculture. International Journal of Microbiology, 2017, 5914786.

Biliavska, L. H., & Prysiazhniuk, O. I. (2018). A model of early-maturing soybean variety. Novitni Agrotehnologiji, 6, 1–15 (in Ukrainian).

Carvalho, E. R., Rocha, D. K., Andrade, D. B. D., Pires, R. M. D. O., Penido, A. C., & Reis, L. V. (2020). Phytotoxicity in soybean seeds treated with phytosanitary products at different application times. Journal of Seed Science, 42, e202042036.

Chehova, І. V. (2021). Formuvannja ta rozvytok rynku olіjnykh kul’tur: Teorіja, metodologіja, praktika [Formation and development of the market of oilseeds: Theory, methodology, practice]. Agrarna Nauka, Kyiv (in Ukrainian).

Ciampitti, I. A., & Salvagiotti, F. (2018). New insights into soybean biological nitrogen fixation. Agronomy Journal, 110(4), 1185–1196.

Dias, M. (2012). Phytotoxicity: An overview of the physiological responses of plants exposed to fungicides. Journal of Botany, 2012, 135479.

Erohin, A. I. (2019). Effektivnost’ primenenija preparata na osnove lektinov zernobobovyh kul’tur v predposevnoj obrabotke semian i vegetirujushhih rastenij goroha [The effectiveness of the preparation on the basis of lectins on leguminous crops in presowing treatment of seeds and vegetating pea plants]. Legumes and Groat Crops, 30, 48–53 (in Russian).

Fernаndez-Ortuno, D., Tores, J. A., De Vicente, A., & Perez-Garcia, A. (2008). Mechanisms of resistance to QoI fungicides in phytopathogenic fungi. International Microbiology, 11(1), 1–9.

Garcia, P. C., Rivero, R. M., Ruiz, J. M., & Romeo, L. (2003). The role of fungicides in the physiology of higher plants: Implications for defense responses. Botanical Review, 69(2), 162–172.

Gorshkov, A. P., Tsyganova, A. V., Vorobiev, M. G., & Tsyganov, V. E. (2020). The fungicide tetramethylthiuram disulfide negatively affects plant cell walls, infection thread walls, and symbiosomes in pea (Pisum sativum L.) symbiotic nodules. Plants, 9(11), 1488.

Guo, Y., Chen, J., Ren, D., Du, B., Wu, L., Zhang, Y., Wang, Z., & Qian, S. (2021). Synthesis of osthol-based botanical fungicides and their antifungal application in crop protection. Bioorganic and Medicinal Chemistry, 40, 116184.

Gupta, R. C. (2018). Toxicity of fungicides, veterinary toxicology. Elsevier, London.

Hardy, R. W. F., Holsten, R. D., Jackson, E. K., & Burns, R. C. (1968). The acetylene-ethylene assay for nitrogen fixation: Laboratory and field evaluation. Plant Physiology, 43(8), 1185–1207.

Hiscox, J., & Israelstam, R. (1979). А method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57(12), 1332–1334.

Hungria, M., Nogueira, M. A., Campos, L. J. M., Menna, P., Brandi, F., & Ramos, Y. G. (2020). Seed pre-inoculation with Bradyrhizobium as time-optimizing option for large-scale soybean cropping systems. Agronomy Journal, 112(6), 5222–5236.

Ilkiv, L. A., & Stepanusko, Y. V. (2021). Ekonomichni aspekty vyrobnyctva soji v Ukrajini [Economic aspects of soybean production in Ukraine]. Colloquium Journal, 120, 6–8 (in Ukrainian).

Jakl, M., Kovac, I., Zeljkovic, S. C., & Dytrtova, J. J. (2021). Triazole fungicides in soil affect the yield of fruit, green biomass, and phenolics production of Solanum lycopersicum L. Food Chemistry, 351(2021), 129328.

Jiang, S., Ma, Z., & Ramachandran, S. (2010). Evolutionary history and stress regulation of the lectin superfamily in higher plants. BMC Evolutionary Biology, 10(79), 79–103.

Kandelinskaya, O. L., Grischenko, E. R., Ripinskaya, K. J., Aleschenkova, Z. M., Kartizhova, L. E., Kuptsov, V. N., & Kuptsov, N. S. (2015). Rol’ lektinov v reguliacii effektivnosti bobovo-rizobial’nogo simbyoza u liupina [Role of lectins in regulation of legume-rhizobium symbiosis efficiency in lupin]. Botanika, Minsk, 44, 283–290 (in Russian).

Kaplaushenko, A., Knysh, E. H., Panasenko, O. I., Sameliuk, Y. H., Kucheriavyi, Y. M., Shcherbak, M. O., Kaplaushenko, T. M., Rud, A. M., & Hulina, Y. S. (2016). Praktychne znachennia ta zastosuvannia pohidnykh 1,2,4-triazolu [Practical value and use of 1,2,4-triazole derivatives]. ZDMU, Zaporizhzhia (in Ukrainian).

Kots, S., Kiriziy, D., Pavlyshche, A., & Rybachenko, L. (2022). Peculiarities of formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic apparatus in relation to photosynthetic activity under the influence of seed protectant. Journal of Microbiology, Biotechnology and Food Sciences, 2022, e3128.

Kyrychenko, E. V. (2014). Lektyny i diazotrofy – polifunkcional’ni komponenty kompleksnyh biologichnykh kompozycij [Phytolectins and diazotrophs are the polyfunctional components of the complex biological compositions]. Biotechnologia Acta, 7(1), 40–53 (in Ukrainian).

Kyrychenko, O. V., Kots, S. Y., & Pukhtaievych, P. P. (2021). The effectiveness of phytolectins and lectin compositions application for spraying plants during vegetation. Biotechnologia Acta, 14(1), 57–68.

Lagarda-Diaz, I., Guzman-Partida, A., & Vazquez-Moreno, L. (2017). Legume lectins: Proteins with diverse applications. International Journal of Molecular Sciences, 18(6), 1242.

Lamichhane, J. R., You, M. P., Laudinot, V., Barbetti, M. J., & Aubertot, J. (2020). Revisiting sustainability of fungicide seed treatments for field crops. Plant Disease, 104(3), 610–623.

Li, S., Li, X., Zhang, H., Wang, Z., & Xu, H. (2021). The research progress in and perspective of potential fungicides: Succinate dehydrogenase inhibitors. Bioorganic and Medicinal Chemistry, 50, 116476.

Mazur, V. A., Honcharuk, I. V., Didur, I. M., Pantsyreva, H. V., Telekalo, N. V., & Kupchuk, I. M. (2021). Innovacijni aspekty tehnologij vyroshhiuvannia, zberigannia i pererobky zernobobovykh kul’tur [Innovative aspects of technologies for growing, storing and processing legumes]. Nilan-LTD, Vinnytsia (in Ukrainian).

Melnichuk, F. S., Marchenko, O. A., & Retman, M. S. (2015). Cytotoksychna dija fungicydnyh protrujnykiv na prorostky soji [Cytotoxic effect of fungicide seed treatments on soybean seedlings]. Scientific Reports of NULES of Ukraine, 54, 1–13 (in Ukrainian).

Melnikova, E. S. (2020). Ispol’zovanije fungicidnykh protravitelej na kartofele v usloviyakh Central’'nogo Chernozem’ja [The use of fungicidal disinfectants on potatoes in the conditions of the Central Chernozem Region]. Zashchita Kartofelia, 1, 9–10 (іn Russian).

Mishra, G., Kumar, N., Giri, K., & Pandey, S. (2013). In vitro interaction between fungicides and beneficial plant growth promoting Rhizobacteria. Africal Journal of Agricultural Research, 8(45), 5630–5633.

Mohamed, H. I., El-Beltagi, H. S., Aly, A. A., & Latif, H. H. (2018). The role of systemic and non-systemic fungicides on the physiological and biochemical parameters in plant: Implications for defense responses. Fresenius Environmental Bulletin, 27(12), 8585–8593.

Molin, C., Ribeiro, N. R., Matusomoto, M. N., Lütkemeyer, A. J., Bordignon, K. B., Ferreira, M. L. B., Barbieri, M., Deuner, C. C., & Huzar-Novakowiski, J. (2021). Seed treatment for controlling damping-off caused by Globisporangium irregulare and Globisporangium ultimum var. sporangiiferum in soybean from southern Brazil. Crop Protection, 149, 105782.

Morgun, V. V., Priadkina, G. A., Stasik, O. O., & Zborivska, O. V. (2019). Relationships between canopy assimilation surface capacity traits and grain productivity of winter wheat genotypes under drought stress. Agricultural Science and Practice, 6(2), 18–28.

Mostoviak, I. I., & Kravchenko, O. V. (2019). Symbiotychnyj aparat soji na foni vykorystannia riznykh vydiv fungicydiv ta mikrobnogo preparatu [Symbiotic apparatus of soya under the application of different types of fungicides and microbial preparation]. Taurida Scientific Herald, 108(12), 72–77 (in Ukrainian).

Nason, M. A., Farrar, J., & Bartlett, D. (2007). Strobilurin fungicides induce changes in photosynthetic gas exchange that do not improve water use efficiency of plants grown under conditions of water stress. Pest Management Science, 63(12), 1191–1200.

Nleye, T., Sexton, P., Gustafson, K., & Miller, J. M. (2019). Soybean growth stages. іGrow Soybean: Best Management Practices for Soybean Production. SDSU Extension, Brookings.

Pavlovskaya, N. E., & Gagarina, I. N. (2017). Funkcional’naja rol’ lektinov rastenij kak predposylka dlia ikh primenenija v biotehnologii [The physiological properties of plant lectins as a prerequisite for their application in biotechnology]. Himija Rastitelnogo Syrja, 1, 21–35 (in Russian).

Pavlyshche, A. V., Yakimchuk, R. A., Omelchuk, S. V., Zhemoyda, A. V., & Kots, S. Y. (2018). Symbiotic properties and seed productivity of soybean in field conditions under various methods of seeds treatment with fungicides. Plant Physiology and Genetics, 50(4), 358–368 (in Ukrainian).

Petit, A., Fontaine, F., Vatsa, P., Clement, C., & Vaillant-Gaveau, N. (2012). Fungicide impacts on photosynthesis in crop plants. Photosynthesis Reseach, 111(3), 315–326.

Priadkіna, H. A., Shadchyna, T. M., Stasіk, O. O., & Kіrіzіy, D. A. (2015). Fotosintez i produktivnost’ rastenij [Photosynthesis and productivity of plants]. Vol. 3. Logos, Kiev (іn Russian).

Rodrigues, T. F., Bender, F. R., Sanzovo, A. W. S., Ferreira, E., Nogueira, M. A., & Hungria, M. (2020). Impact of pesticides in properties of Bradyrhizobium spp. and in the symbiotic performance with soybean. World Journal of Microbiology and Biotechnology, 36(11), 1–16.

Roman, D. L., Voiculescu, D. I., Filip, M., Ostafe, V., & Isvoran, A. (2021). Effects of triazole fungicides on soil microbiota and on the activities of enzymes found in soil: A review. Agriculture, 11(9), 893.

Saha, A., & Mandal, S. (2019). Nutritional benefit of soybean and its advancement in research. Sustainable Food Production, 5, 6–16.

Saladin, G., Magné, C., & Clément, C. (2003). Effects of fludioxonil and pyrimethanil, two fungicides used against Botrytis cinerea, on carbohydrate physiology in Vitis vinifera L. Pest Management Science, 59(10), 1083–1092.

Sartori, F. F., Pimpinato, R. F, Tornisielo, V. L., Engroff, T. D., Jaccoud‐Filho, D. S, Menten, J. O., Dorrance, A. E., & Dourado‐Neto, D. (2020). Soybean seed treatment: How do fungicides translocate in plants? Pest Management Science, 76(7), 2355–2359.

Sergienko, V. G., Kyrychenko, O. V., & Perkovska, G. Y. (2009). Method of using plant lectins to protect vegetable crops from diseases. Patent UA No 41723 A01N 63/00, A01C 1/06 / zayavnyk i patentovlasnyk Institute of Plant Protection of Ukrainian Academy of Agrarian Sciences. u200812612. Zayavl. 28.10.2008. Оpubl. 10.06.2009. Byul. No 11 (in Ukrainian).

Shahid, M., Khan, M. S., & Zaidi, A. (2020). Fungicide toxicity to legumes and its microbial remediation: A current perspective. Pesticides in Crop Production: Physiological and Biochemical Action, 339(686), 1452–1477.

Silva, K., Silva, E. E., Farias, E. D. N. C., Silva, C. J., Albuquerque, C. N. B., & Cardoso, C. (2018). Agronomic efficiency of Bradyrhizobium pre-inoculation in association with chemical treatment of soybean seeds. African Journal of Agricultural Research, 13(14), 726–732.

Singh, G., & Sahota, H. K. (2018). Impact of benzimidazole and dithiocarbamate fungicides on the photosynthetic machinery, sugar content and various antioxidative enzymes in chickpea. Plant Physiology and Biochemistry, 132, 166–173.

Sytnikov, D. M., Kots, S. Y., Malichenko, S. M., & Kirizii, D. A. (2006). Photosynthetic rate and lectin activity of soybean leaves after inoculation with rhizobia together with homologous lectin. Russian Journal of Plant Physiology, 53(2), 169–175.

Tarnovskyi, M. H., & Yankovskyi, Y. Y. (2012). Optychni metody analizu fiziologichnogo stanu roslyn dlja zadach sil’s’kogo gospodarstva ta ekologichnogo monitoryngu [Optical methods for the analysis of the physiological state of plants for the tasks of agriculture and ecological monitoring]. Optoelectronic Information – Power Technologies, 23(1), 127–130 (in Ukrainian).

Tkalich, Y. I., Tsyliuryk, O. І., Rudakov, Y. M., & Kozechko, V. І. (2021). Efficiency of post-emergence (“insurance”) herbicides in soybean crops of the Northern Steppe of Ukraine. Agrology, 4(4), 165–173.

Turenko, V. P. (2019). Current fungicides in the protection of cereals. Agronom, 4(12), 1–2 (in Ukrainian).

Vozniuk, S. V., Tytova, L. V., Liaska, S. I., & Iutynska, H. O. (2015). Vplyv fungicydiv ta kompleksnogo inokuljantu Ekovital na ryzosfernyj mikrobiocenoz, stijkist’ do zakhvoriuvan’ ta produktyvnist’ soji [Effect of fungicides and complex inoculant Ekovital on rhizospheric microbiocenosis, disease resistance and productivity]. Microbiological Journal, 77(4), 8–14 (іn Ukrainian).

Vyshnivskyi, P. S., & Furman, O. V. (2020). Soybean productivity depending on elements of growing technology in the right-bank forest-steppe of Ukraine. Plant and Soil Science, 11(1), 13–22.

Wellburn, A. (1994). The spectral determination of chlorophylls a and b as well as total carotenoids using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144(3), 307–313.

Yashchuk, V. U., Vashchenko, V. M., Koretskyi, A. P., Kryvosheia, R. M., & Chaikovska V. V. (2016). Perelik pestytsydiv i ahrokhimikativ, dozvolenykh do vykorystannia v Ukraini [List of pesticides and agrochemicals approved for use in Ukraine]. Iunivest Media, Kyiv (in Ukrainian).

Zhang, C., Zhou, T., Xu, Y., Du, Z., Li, B., Wang, J., Wang, J., & Zhu, L. (2020). Ecotoxicology of strobilurin fungicides. Science of the Total Environment, 742(2020), 140611.

Published
2022-04-05
How to Cite
Kyrychenko, O. V., Kots, S. Y., Khrapova, A. V., & Omelchuk, S. V. (2022). Biological activity of soybean seed lectin at the spraying of Glycine max plants against the background of seed treatment with pesticide containing fipronil, thiophanate-methyl, pyraclostrobin as active substances and rhizobial bacterization . Regulatory Mechanisms in Biosystems, 13(2), 105-113. https://doi.org/10.15421/022215