The experimental invasion of rats with Eustrongylides excisus (Nematoda, Dioctophymatidae) larvae during the acute course of infection
AbstractTen laboratory rats were experimentally invaded per os with the Eustrongylides excisus (Jäegerskiold, 1909) nematode larvae (L3–L4) received from Perca fluviatialis (Linnaeus, 1758), caught in the water area of the Dnipro-Buh Estuary, Ukraine. The aim of the experimental study was to measure the pathogenic effect of the given parasite on the mammals’ organisms by means of the histological analysis of the affected organs of the infected rats. The researched animals were divided into two groups. The experiment lasted 15 days; the second group was a control group. The histological analysis showed purulent-serous and purulent-fibrinous peritonitis, acute circulatory disorders in brain tissues (signs of hypoxic-ischemic lesions of the brainstem in the form of neuronal changes) and the heart (partial spasm of arteries and edema of interstitial myocardial tissue); pathological changes in the lungs (acute interstitial purulent pneumonia, acute focal emphysema of the lungs), liver (acute exudative hepatitis), kidneys (signs of shock reaction) and intestines (acute serous-purulent enteritis). Formation of non-specific granulomas was noticed, which contained the fragments of the parasitic larvae. The acute course of the purulent-serous and purulent-fibrinous inflammatory processes in the organs of the invaded rats was noticed; signs of systemic inflammatory response syndrome with pronounced distributive vascular leukocytosis in the studied organs.
Barros, L. A., Tortelly, R., Pinto, R. M., & Gomes, D. C. (2004). Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882) Baylis, 1920 in rabbits. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, 56, 325–332.
Boyko, A. A., & Brygadyrenko, V. V. (2017). Changes in the viability of the eggs of Ascaris suum under the influence of flavourings and source materials approved for use in and on foods. Biosystems Diversity, 25(2), 162–166.
Boyko, O. O., & Brygadyrenko, V. V. (2019). Nematocidial activity of aqueous solutions of plants of the families Cupressaceae, Rosaceae, Asteraceae, Fabaceae, Cannabaceae and Apiaceae. Biosystems Diversity, 27(3), 227–232.
Branciari, R., Ranucci, D., Miraglia, D., Valiani, A., Veronesi, F., & Urbani, E. (2016). Occurrence of parasites of the genus Eustrongylides spp. (Nematoda: Dioctophymatidae) in fish caught in Trasimeno Lake, Italy. Italian Journal of Food Safety, 6130, 206–209.
Bruschi, F., & Chiumiento, L. (2011). Trichinella inflammatory myopathy: Host or parasite strategy? Parasit and Vectors, 4, 42.
Cole, R. A. (1999). Eustrongylidosis. In: Field manual of wildlife diseases: General field procedures and diseases of birds. U.S. Department of the interior fish and wildlife service, Washington. Pp. 223–228.
Deardorff, T. L., & Overstreet, R. M. (1991). Sea food transmitted zoonoses in the United States: The fishes, the dishes, and the worms. In: Ward, D. R., & Hackney, C. R. (Eds.). Microbiology of marine food products. Van Nostrand Reinhold, New York. Pp. 211–265.
Dezfuli, B. S., Manera, M., Lorenzoni, M., Pironi, F., Shinn, A. P., & Giari, L. (2015). Histopathology and the inflammatory response of European perch, Perca fluviatilis muscle infected with Eustrongylides sp. (Nematoda). Parasit and Vectors, 8, 227.
Eberhard, M. L., & Ruiz-Tiben, E. (2014). Cutaneous emergence of Eustrongylides in two persons from South Sudan. American Journal of Tropical Medicine and Hygiene, 90, 315–317.
Eberhard, M. L., Hurwitz, H., Sun, A. M., & Coletta, D. (1989). Intestinal perforation caused by larval Eustrongylides (Nematoda: Dioctophymatoidae) in New Jersey. American Journal of Tropical Medicine and Hygiene, 40, 648–650.
El-Dakhly, K. M., El-Nahass, E., Uni, S., Tuji, H., Sakai, H., & Yanai, T. (2012). Levels of infection of gastric nematodes in a flock of great cormorants (Phalacrocorax carbo) from Lake Biwa, Japan. Journal of Helminthology, 86, 54–63.
Fedorov, N. M., Firsov, N. F., & Solovyov, N. A. (2014). Veterinary and sanitary examination of river perch with eustrongylidosis [Veterinarno-sanitarnaya ekspertiza rechnogo okunya pri eustrongilidoze]. Veterinarnaya Patologiya, 3–4, 68–73 (in Russian).
Franson, J. C., & Cluste, T. W. (1994). Eustronglyidosis in wading birds from colonies in California, Texas, and Rhode Island, USA. Colonial Waterbirds, 17(2), 168–172.
Goncharov, S. L., Soroka, N. M., Pashkevich, I. Y., Dubovyi, A. I., & Bondar, A. O. (2018). Infection of predatory fish with larvae of Eustrongylides excisus (Nematoda, Dioctophymatidae) in the delta of the Dnipro River and the Dnipro-Buh Estuary in Southern Ukraine. Vestnik Zoologii, 52(2), 137–144.
Guagliardo, S., Viozzi, G., & Norma, B. (2019). Pathology associated with larval Eustrongylides sp. (Nematoda: Dioctophymatoidea) infection in Galaxias maculatus (Actinopterygii: Galaxiidae) from Patagonia, Argentina. International Journal for Parasitology: Parasites and Wildlife, 10, 113–116.
Guerin, P. F., Marapendi, S., & Grail, S. L. (1982). Intestinal perforation caused by larval Eustrongylides – Maryland. Morbidity and Mortality Weekly Report, 31, 383–384.
Gunby, P. (1982). One worm in the minnow equals too many in the gut. Journal American Medical Association, 248, 163.
Guseva, E. Y., Zhuravleva, Y. A., & Zotova, N. V. (2019). Correlation of immunity evolution and inflammation in vertebrates [Korrelyaciya evolyucii immuniteta i vospaleniya u pozvonochnyh]. Uspehi Sovremennoj Biologii, 139(1), 1–16 (in Russian).
Honcharov, S. L. (2020). Pathological anatomic changes among laboratory rats in case of experimental infection with the larvae of the nematode Eustrongylides excisus (Nematoda: Dioctophymatidae). Animal Biology, 22(1), 30–37.
Jägerskiöld, L. A. (1909). Zur Kenntnis der Nematoden-Gattungen Eustrongylides und Hystrichis. Nova Acta Regiae Societatis Scientiarum Upsaliensis, 4(2), 3 (in German).
Karmanova, E. M. (1968). Dioctophymidea of animals and man and the diseases caused by them [Dioktifimidei zhivotnyh i cheloveka i vyzyvaemye imi zabolevaniya]. Nauka, Moscow (in Russian).
Lichtenfels, J. R., & Stroup, C. F. (1985). Eustrongylides sp. (Nematoda: Dioctophymatoidea): First report of an invertebrate host (Oligochaeta: Tubificidae) in North America. Proceedings of the Helminthological Society of Washington, 52(2), 320–323.
Ljubojevica, D., Novakov, N., Djordjevicc, V., Radosavljevicd, V., & Pelica, M. (2015). Potential parasitic hazards for humans in fish meat. Procedia Food Science, 5, 172–175.
McDougald, L. R. (2019). Internal parasites. In: Swayne, D. E. et al. (Eds.). Diseases of poultry. Wiley. Pp. 1157–1191.
Melo, F. T., Melo, C. S., & Nascimento, L. C. (2015). Morphological characterization of Eustrongylides ssp. larvae (Nematoda, Dioctophymatoidea) parasite of Rhinella marina (Amphibia: Bufonidae) from Eastern Amazonia. Brazilian Journal of Veterinary Parasitology, 25(2), 7–12.
Narr, L. L., O’Donnell, J. G., Libster, B., Alessi, P., & Abraham, D. (1996). Eustrongylidiasis – a parasitic infection acquired by eating live minnows. Journal of the American Osteopathic Association, 96, 400–402.
Noei, M. R., Ibrahimov, S., & Sattari, M. (2015). Parasitic worms of the Persian sturgeon, Acipenser persicus Borodin, 1897 from the southwestern shores of the Caspian Sea. Iranian Journal of Ichthyology, 2, 287–295.
Novakov, N., Bjelic-Cabrilo, O., Circovic, M., Jubojevik, D., & Lujic, J. (2013). Eustrongylidosis of european catfish (Siluris glaris). Bulgarian Journal of Agricultural Science, 1, 72–76.
Panesar, T. S., & Beaver, P. C. (1979). Morphology of the advanced-stage larva of Eustrongylides wenrichi Canavan 1929, occurring encapsulated in the tissues of amphiuma in Louisiana. Journal of Parasitology, 65(1), 96–104.
Pazooki, J., Masoumian, M., Yahyazadeh, M., & Abbasi, J. (2007). Metazoan parasites from freshwater fishes of northwest Iran. Journal of Agriculture, Science and Technology, 9, 25–33.
Rohlenová, K., Morand, S., Hyršl, P., Tolarová, S., Flajšhans, M., & Šimková, A. (2011). Are fishimmune systems really affected by parasites? An immunoecological study of common carp (Cyprinus carpio). Parasit and Vectors, 4, 120.
Shirazian, D., Schller, E. L., Glaser, C. A., & Vondeerfecht, S. L. (1984). Pathology of larval Eustrongylides in the rabbit. Journal of Parasitology, 70(5), 803–806.
Soylu, E. (2013). Metazoan parasites of perch Perca fluviatilis L. From Lake Sığırcı, Ipsala, Turkey. Pakistan Journal of Zoology, 45(1), 47–52.
Spalding, M. G., Bancroft, G. T., & Forrester, D. J. (1993). The epizootiology of eustrongylidosis in wading birds (Ciconiiformes) in Florida. Journal of Wildlife Diseases, 29, 237–249.
Ventura, A. S., Ishikawa, M. M., Gabriel, A. M. A., Silbiger, H. L. N., Cavichiolo, F., & Takemoto, R. M. (2016). Histopathology from liver of tuvira (Gymnotus spp.) parasitized by larvae of nematodes. Ciencia Rural, 47, 1233–1239.
Wittner, M., Turner, J. W., Jacquette, G., Ash, R. L., Salgo, M. P., & Tanowitz, H. B. (1989). Eustrongylidiasis – a parasitic infection acquired by eating sushi. The England Journal of Medicine, 320, 1124–1126.
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