Prophylaxis of microelementosis in rabbits using a mixture of glauconite, succinic, humic and fulvic acids and minerals

  • Y. V. Korniichuk National University of Life and Environmental Sciences of Ukraine
  • N. H. Grushanska National University of Life and Environmental Sciences of Ukraine
  • V. M. Kostenko National University of Life and Environmental Sciences of Ukraine
  • T. A. Paliukh National University of Life and Environmental Sciences of Ukraine
  • I. F. Makovska National University of Life and Environmental Sciences of Ukraine
Keywords: microelements; blood of rabbits; New Zealand white rabbits; atomic emission spectrometry; TBA-active product; catalase.

Abstract

Impaired metabolism of mineral substances in the conditions of industrial rabbit breeding may cause decrease in increment of live mass, reduction of immunity, mass morbidity and death of animals. In our experiment, we studied the efficiency of using a mineral mixture to prevent the disorders in the metabolism of rabbits according to changes in morphologic, biochemical parameters and antioxidant status of blood, chemical composition of blood plasma and increments in body weight compared to the control group of animals. For the studies, we formed four groups of white New Zealand rabbits, each comprising six individuals aged 70 days with mean body weight equaling 1.99 kg. In group I, the rabbits received an aqueous form of a mixture of glauconite, succinic acid, humic and fulvic acids and lactates of zinc, manganese, cuprum, cobalt and iron with water, rabbits of group II were given a dry form of a mixture of glauconite, succinic acid, humic and fulvic acids and lactates of zinc, manganese, cuprum, cobalt and iron with fodder, and the rabbits of group III were intramuscularly injected with butanol fraction of humic acids. The experiment lasted for 21 days. The results of the experiment indicate that the most effective prophylaxis of malfunctions of mineral metabolism in white New Zealand rabbits aged 70–95 was dry mixture of glauconite, succinic acid, humic and fulvic acids and lactates of zinc, manganese, cuprum, cobalt and iron with fodder (group II), which was given once a day for 21-day period. We determined a positive effect of biologically active supplement on the parameters of hematopoiesis(1.25 times significantly higher level of hemoglobin and 1.14 times higher number of erythrocytes), metabolism of proteins (1.54 times significantly higher content of albumins), mineral substances (significantly higher content of inorganic phosphorus – by 1.17 times, calcium by 2.18 times, manganese by 1.39 times, zinc by 1.50 times, iron by 1.39 times and cuprum by 1.49 times), functional condition of the liver (2.04 times lower activity of gamma-glutamyltransferase), the state of the antioxidant system (lowest catalase activity) and energy of rabbits’ growth (1.20-fold increment in body weight). The results of our study indicate that using a dry form of the mixture of glauconite, succinic acid, humic and fulvic acids and lactates of zinc, manganese, cuprum, cobalt and iron with fodder is an efficient method of preventing malfunctioning of mineral metabolism in rabbits.

References

Abd El-Rahim, M. I. (2017). The role of nutrition in immunity and diseases resistance in rabbits. Egyptian Journal of Rabbit Science, 27(2), 171–195.

Abdel-Azeem, S., Basyony, M. M., & Abu Hafsa, S. (2019). Feed intake, antioxidant properties and litter performance affected by multi-nutrient block additive of rabbit does during prevailing heat stress in Egypt. Journal Animal and Poultry Production, 10(5), 133–139.

Abdel-Azeem, S., Hassan, A. A., & Abu Hafsa, S. (2019). Body weight response, milk production and lipid peroxidation of rabbit does to multi-nutrient block supplementation during summer conditions. Journal Animal and Poultry Production, 10(5), 157–163.

Abdel-Hamid, T. M., & Farahat, M. H. (2016). Effect of dietary mannan-olygosaccharides on some blood biochemical, haematological parameters and carcass traits in purebred New Zealand White and crossbred rabbits. Animal Production Science, 56, 2133–2139.

Al-Sagheer, A. A., Abdel-Rahman, G., Ayyat, M. S., Gabr, H. A., & Elsisi, G. F. (2020). Productive performance response of growing rabbits to dietary protein reduction and supplementation of pyridoxine, protease, and zinc. Annals of the Brazilian Academy of Sciences, 92(3), e20180989.

Brechka, N., Bondarenko, V., Morozenko, D., Grushanska, N., Sharandak, P., Selukova, N., & Danilchenco, S. (2019). The state of prooxidant-antioxidant balance in prostate gland of rats with cryo-trauma and its correction with drugs of natural origin. Georgian Medical News, 296, 91–95.

Carpenter, J. W., & Marion, C. J. (2018). Exotic animal formulary. Fifth edition. Elsevier, Boston.

Casado, C., Moya, V. J., Pascual, J. J., Blas, E., & Cervera, C. (2011). Effect of oxidation state of dietary sunflower oil and dietary zinc and α-tocopheryl acetate supplementation on performance of growing rabbits. World Rabbit Science, 19, 191–202.

Clauss, M., & Hatt, J. M. (2017). Evidence-based rabbit housing and nutrition. Exotic Animal Practice, 20(3), 871–884.

Cobanova, K., Chrastinova, L., Chrenkova, M., Polacikova, M., Formelova, Z., Ivanisinova, O., Ryzner, M., & Gresakova, L. (2018). The effect of different dietary zinc sources on mineral deposition and antioxidant indices in rabbit tissues. World Rabbit Science, 26, 241–248.

Dychok, A. Z., Lesyk, J. V., & Tsap, M. M. (2018). Rezystentnist’ organizmu kroliv za dii spoluk sulfuru [The resistance of rabbit organism for the effect of sulfur complex]. The Animal Biology, 20(3), 16–23 (in Ukrainian).

Ebeid, T. A., Zeweil, H. S., Basyony, M. M., Dosoky, W. M., & Badry, H. (2013). Fortification of rabbit diets with vitamin E or selenium affects growth performance, lipid peroxidation, oxidative status and immune response in growing rabbits. Livestock Science, 155, 323–331.

Elokil, A. A., Imbabi, T. A., Mohamed, H. I., Abouelezz, K. F. M., Ahmed-Farid, O., Shishay, G., Sabike, I. I., & Liu, H. (2019). Zinc and copper with new triazine hydrazone ligand: Two novel organic complexes enhanced expression of peptide growth factors and cytokine genes in weaned v-line rabbit. Animals, 9(12), 1134.

Fedorchenko, M. M. (2016). Dejaki pokaznyky antyoksydantnogo zahystu u plazmi krovi ta pechinci kroliv [Some indicators of antioxidant protection in blood plasma and liver of rabbits]. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 65, 147–152 (in Ukrainian).

Fedoruk, R. S., & Lesyk, J. V. (2009). Osoblyvosti zhyvlennia kroliv za suchasnyh metodiv vedennia krolivnyctva [Features of rabbit nutrition with modern methods of rabbit breeding]. The Animal Biology, 11, 91–103 (in Ukrainian).

Filippova, O. B., Kijko, E. I., & Maslova, N. I. (2019). Sorbcija metallov na glaukonite v uslovijah zheludochno-kishechnogo trakta teljat [Sorption of metals on glauconite in conditions of the gastrointestinal tract of calves]. Russian Agricultural Science, 5, 44–48 (in Russian).

Gidenne, T. (2015). Dietary fibres in the nutrition of the growing rabbit and recommendations to preserve digestive health: A review. Animal, 9(2), 227–242.

Gidenne, T., Fortun-Lamothe, L., Bannelier, C., Molette, C., Gilbert, H., Chemit, M. L., Segura, M., Benitez, F., Richard, F., Garreau, H., & Drouilhet, L. (2017). Direct and correlated responses to selection in two lines of rabbits selected for feed efficiency under ad libitum and restricted feeding: III. Digestion and excretion of nitrogen and minerals. Journal Animal Science, 95, 1301–1312.

Grushanska, N. H. (2017). Vmist vazhkykh metaliv u shersti koriv pivnichno-skhidnoji bioheokhimichnoji zony [The content of heavy metals in the hair of cows in the northern-eastern biogeochemical zone]. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, Veterinary Sciences, 73, 154–158 (in Ukrainian).

Hall, E. R. (1952). Investigations on the microbiology of cellulose utilization in domestic rabbits. Journal Microbiology, 7, 350–357.

Hassan, F., Mobarez, S., Mohamed, M., Attia, Y., Mekawy, A., & Mahrose, K. (2021). Zinc and/or selenium enriched Spirulina as antioxidants in growing rabbit diets to alleviate the deleterious impacts of heat stress during summer season. Animals, 11(3), 756.

Jain, N. C. (1986). Schalm’s veterinary hematology. 4th edition. Lea & Febiger, Philadelphia.

Kamel, D., Abdel-Khalek, A., & Gabr, S. (2020). Effect of dietary zinc-oxide or nanj-zinc oxide on growth performance, oxidative stress, and immunity of growing rabbits under hot climate conditions. Journal of Animal and Poultry Production, 11(12), 565–571.

Kiwull-Schöne, H., Kalhoff, H., Manz, F., & Kiwull, P. (2005). Food mineral composition and acid-base balance in rabbits. European Journal of Nutrition, 44(8), 499–508.

Kunkel, H. O., & Pearson, P. B. (1948). Magnesium in the nutrition of the rabbit. The Journal of Nutrition, 36(6), 657–666.

Lieshchova, M. A., Bilan, M. V., Bohomaz, A. A., Tishkina, N. M., & Brygadyrenko, V. V. (2020). Effect of succinic acid on the organism of mice and their intestinal microbiota against the background of excessive fat consumption. Regulatory Mechanisms in Biosystems, 11(2), 153–161.

Maha, S. A. Salama, Wael, A. M. Morsy, Radi, A. Mohamed, & Samy, A. El-Midany. (2019). Effect of some feed-additives on the growth performance, physiological response and histopatological changes of rabbits subjected to ochratoxin-A feed contamination. Slovenian Veterinary Research, 56(22), 499–508.

Mattioli, S., Rosignoli, P., D’Amato, R., Fontanella, M. C., Regni, L., Castellini, C., Proietti, P., Elia, A. C., Fabiani, R., Beone, G. M., Businelli, D., & Dal Bosco, A. (2020). Effect of feed supplemented with selenium-enriched olive leaves on plasma oxidative status, mineral profile, and leukocyte DNA damage in growing rabbits. Animals, 10(2), 274.

Mista, D., Rzasa, A., Szmanko, T., Zawadzki, W., Styczynska, M., Pintal, A., & Kroliczewska, B. (2012). The effect of humic-fatty acid preparation on production parameters and meat quality of growing rabbits. Annals of Animal Science, 12(1), 117–126.

Pedan, L. R. (2013). Profilaktyka vplyvu chynnykiv navkolyshnogo seredovyshha na zdorov’ja za dopomogoju mikroelementu margancju (ogljad literatury) [Prevention of the impact of environmental factors on health with the help of the trace element manganese (literature review)]. Gigiene of Settlements, 62, 326–345 (in Ukrainian).

Proença, L. M., & Mayer, J. (2014). Prescription diets for rabbits. Exotic Animal Practice, 17(3), 485–502.

Raafat, B. M., El-Barbary, A., Tousson, E., & Aziz, S. W. (2011). Di-mercapto succinic acid (DMSA) and vitamin C chelating potency in lead intoxication, regarding oxidative stress and apoptotic related proteins in rabbits. Journal of Genetic Engineering and Biotechnology, 9(2), 121–131.

Romanchuk, L. D., & Annamuhamedova, O. O. (2002). Vplyv mikroelementnyh dobavok na okremi pokaznyky fiziologichnogo statusu vidgodivel’nogo molodnjaka v umovah dovgotryvalogo radiacijnogo zabrudnennja u malyh dozah [Influence of microelement additives on separate indicators of physiological status of fattening young in the conditions of long-term radiation pollution in small doses]. Bulletin of the State Agroecological University (Zhytomyr), 2, 90–94 (in Ukrainian).

Shtapenko, O. V., Gevkan, I. I., Slyvchuk, Y. I., Dzen, Y. O., Syrvatka, V. Y., & Matvienko, N. M. (2018). Effect of organic microelements in liposomal form on fertilizing ability and the level of antioxidant reactions of female rabbits. Biotechnologia Acta, 11(4), 50–56.

Sychov, M. J., Holubieva, T. A., Pozniakovskyi, J. V., Andriienko, L. M., & Holubiev, M. I. (2018). Produktyvnist’ molodnjaku kroliv za riznyh rivniv metioninu v kombikormah [Productivity of young rabbits at different levels of methionine in feed]. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 84, 60–64 (in Ukrainian).

Tsekhmistrenko, S. I., & Fedorchenko, M. M. (2015). Vplyv vitaminno-mineral’noji dobavky na pokaznyky peroksydnogo okyslennja lipidiv v organizmi kroliv [The effect of vitamin and mineral supplements on lipid peroxidation in rabbits]. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 16, 249–255 (in Ukrainian).

Yan, J. Y., Zhang, G. W., Zhang, C., Tang, L., & Kuang, S. Y. (2017). Effect of dietary organic zinc sources on growth performance, incidence of diarrhoea, serum and tissue zinc concentrations, and intestinal morphology in growing rabbits. World Rabbits Science, 25, 43–49.

Zadnipryany, I. V., Sataieva, T. P., Tretiakova, O. S., & Zukow, W. (2019). Miocardial interstitial matrix as novel target for succinic acid treatment strategies during experimental hypobaric hypoxia. Russian Open Medical Journal, 8(2), e0201.

Published
2021-09-02
How to Cite
Korniichuk, Y. V., Grushanska, N. H., Kostenko, V. M., Paliukh, T. A., & Makovska, I. F. (2021). Prophylaxis of microelementosis in rabbits using a mixture of glauconite, succinic, humic and fulvic acids and minerals . Regulatory Mechanisms in Biosystems, 12(3), 571-579. https://doi.org/10.15421/022178

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