Effect of Heracleum sosnowskyi extract aqueous solution on the Allium cepa root meristem

  • M. V. Smirnova Kola Science Center of the Russian Academy of Sciences
  • V. A. Kotelnikov Kola Science Center of the Russian Academy of Sciences
Keywords: genotoxicity; mitosis; plant extract; mitotic activity; lagging and sticking chromosomes; anaphase bridges; ring chromosomes; nuclear buds


Heracleum sosnowskyi (Apiaceae) contains a lot of useful chemical ingredients that can be used in industry, medicine and other fields as plant component extracts and as chemical compounds that have been extracted in different ways, which requires the last to be tested for chemical safety, including a genotoxic test in vivo. In the present paper, the 96-hour effect of the H. sosnowskyi extract aqueous solution at concentrations of 0.01, 0.05, 0.10, and 0.50 mL/L on the genetic apparatus and mitotic activity of the cells of the Allium cepa (Alliaceae) root meristem is discussed. Distilled water was applied as a negative control, and hydrogen peroxide 1% as a positive one. The extract was prepared from the plant’s fresh leaves by soaking them in acetone. It was then distilled at 57 ºС and diluted with distilled water to obtain the experimental concentrations. As extract content in the aqueous solution increased, a statistically significant decrease in mitotic activity, an increase in aberrant cell percentage and a concentration-dependent inhibition of root growth were observed. In the 0.5 mL/L solution, if compared against the other experimental concentrations, an increase in the metaphase, anaphase and telophase indices along with a decrease in the prophase index were observed. The most common aberrations for all the concentrations were lagging and sticking chromosomes, anaphase bridges, ring chromosomes and nuclear buds. The same solution and the positive control produced membrane damage; giant and ghost cells. The results of the experiment performed have demonstrated the extract’s aneugenic effect that causes spindle disturbance, mitodepression and inhibits the cells of the Allium cepa root meristem, prevails over its clastogenic effect.


Akinboro, A., & Bakare, A. A. (2007). Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa L. Journal Ethnopharmacology, 112(3), 470–475.

Akwu, N. A., Naidoo, Y., & Singh, M. (2019). Cytogenotoxic and biological evaluation of the aqueous extracts of Grewia lasiocarpa: An Allium cepa assay. South African Journal of Botany, 125, 371–380.

Bahadori, M. B., Dinparast, L., & Zengin, G. (2016). The genus Heracleum: A comprehensive review on its phytochemistry, pharmacology, and ethnobotanical values as a useful herb. Comprehensive Reviews in Food Science and Food Safety, 15(6), 1018–1039.

Bogucka-Kocka, A., Smolarz, H., & Kocki, J. (2008). Apoptotic activities of ethanol extracts from some Apiaceae on human leukemia cell lines. Fitoterapia, 79, 487–497.

Bonciu, E., Firbas, P., Fontanetti, C. S., Wusheng, J., Karaismailoğlu, M. C., Liu, D., Menicucci, F., Pesnya, D. S., Popescu, A., Romanovsky, A. V., Schiff, S., Ślusarczyk, J., De Souza, C. P., Srivastava, A., Sultan, A. O., & Papini, A. (2018). An evaluation for the standardization of the Allium cepa test as cytotoxicity and genotoxicity assay. Caryologia, 71(3), 191–209.

Christopher, H. B., & Kapoor, M. B. (1988). The cytogenetic effects of sodium Salicylate on the root meristem cells of Allium sativa L. Cytologia, 54, 203–209.

Cock, M. J. W., Eckstein, L., Evans, H. C., & Fröberg, L. (2007). Ecology and management of giant hogweed (Heracleum mantegazzianum). CABI, Institute of Botany, Academy of Sciences of the Czech Republic, город?.

De Abreu, J. C., De Santana, R. A., Ribeiro, G. R. H., Dantas, M. M., Guisso, L. F., Valente, L. L., Luiz Silva, W. S., Silva, D. R., Barbieri, R. S., & Da Costa, N. M. A. (2019). Effects cytotoxic and genotoxic of aqueous extract of fennel (Foeniculum vulgare var. vulgare Mill.). International Journal of Advanced Engineering Research and Science, 6(3), 230–236.

El-Ghamery, A. A., El-Nahas, A. I., & Mansour, M. M. (2000). The action of atrazine herbicide as an inhibitor of cell division on chromosomes and nucleic acids content in root meristems of Allium cepa L. and Vicia faba L. Cytologia, 65(3), 277–287.

Fenech, M., Kirsch-Volders, M., Natarajan, A. T., Surralles, J., Crott, J. W., Parry, J., Norppa, H., Eastmond, D. A., Tucker, J. D., & Thomas, P. (2011). Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis, 26(1), 125–132.

Fernandes, T. C. C., Mazzeo, D. E. C., & Marin-Morales, M. A. (2007). Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifluralin herbicide. Pesticide Biochemistry and Physiology, 88(3), 252–259.

Firuzi, O., Asadollahi, M., Gholami, M., & Javidnia, K. (2010). Composition and biological activities of essential oils from four Heracleum species. Food Chemistry, 122, 117–122.

Fiskesjö, G. (1985). The Allium-test as a standart in environmental monitoring. Hereditas, 102(1), 99–112.

Gao, Y., Liu, Y., Wang, Z., & Zhang, H. (2014). Chemical constituents of Heracleum dissectum and their cytotoxic activity. Phytochemistry Letters, 10, 276–280.

Gaulden, M. E. (1987). Hypothesis: Some mutagens directly alter specific chromosomal proteins (DNA topoisomerase 11 and peripheral proteins) to produce chromosome stickiness, which causes chromosome aberrations. Mutagenesis, 2, 357–365.

Georgievskij, V. P., Komissarenko, N. F., & Dmitruk, S. E. (1990). Biologicheski aktivnye veshhestva lekarstvennykh rastenij [Biologically active substances of medicinal plants]. Novosibirsk, Nauka (in Russian).

Gömürgen, A. N. (2005). Cytological effect of the potassium metabisulphite and potassium nitrate food preservative on root tip of Allium cepa L. Cytologia, 70(2), 119–128.

Grant, W. F. (1982). Chromosome aberration assays in Allium. Mutation Research, Fundamental and Molecular Mechanisms of Mutagenesis, 99, 273–291.

Grant, W. F. (1994). The present status of higher plant bioassays for the detection of environmental mutagens. Mutation Research, Fundamental and Molecular Mechanisms of Mutagenesis, 310(2), 175–185.

Hosseinzadeh, Z., Ramazani, A., & Razzaghi-Asl, N. (2019). Plants of the genus Heracleum as a source of coumarin and furanocoumarin. Journal of Chemical Reviews, 1(2), 78–98.

Jahodová, S., Trybush, S., Pysek P., Wade, M., & Karp, A. (2007). Invasive species of Heracleum in Europe: An insight into genetic relationships and invasion history. Diversity and Distributions, 13(1), 99–114.

Jakubska-Busse, A., Sliwinski, M., & Kobylka, M. (2013). Identification of bioactive components of essential oils in Heracleum sosnowskyi and Heracleum mantegazzianum (Apiaceae). Archives of Biological Sciences, 65(3), 877–883.

Karpova, S. S., Аl’-Khachami, F. R. H., Kalaev, V. N., Ignatova, I. V., & Slavskij, V. А. (2020). Tsitogeneticheskij polimorfizm semennogo potomstva derevjev orekha gretskogo (Juglans regia L.) pri introduktsii v Voronezhskoj oblasti. [Cytogenetic polymorphism of walnut trees (Juglans regia L.) seed progeny during introduction in the Voronezh region]. Ekologiya Urbanizirovannykh Territorij, 2, 21–29 (in Russian).

Kenne, K., Ljungquist, S., & Ringertz, N. R. (1986). Effects of asbestos fibers on cell division, cell survival and formation of thioguanine resistant mutants in Chinese hamster ovary cells. Environmental Research, 39, 448–464.

Kurás, L. (2004). Characterization of protei-DNA association in vivo by chromatin immunoprecipitation. Methods of Molecular Biology, 284, 147–162.

Laman, N. А., & Usik, А. V. (2020). Lokalizatsiya i sostav kumarinov v kornyakh borshhevika sosnovskogo (Heracleum sosnowskyi Manden.) [Localization and composition of coumarins in roots of Heracleum sosnowskyi Manden.]. Izvestiya Natsional’noj Akademii Nauk Belarusi, Seriya Biologicheskikh Nauk, 65(1), 71–75 (in Russian).

Leme, D. M., & Marin-Morales, M. A. (2009). Allium cepa test in environmental monitoring: A review on its application. Mutation Research, Reviews in Mutation Research, 682(1), 71–81.

Li, D., Huang, Q., Lu, M., Zhang, L., Yang, Z., Zong, M., & Tao, L. (2015). The organophosphate insecticide chlorpyrifos confers its genotoxic effects by inducing DNA damage and cell apoptosis. Chemosphere, 135, 387–393.

Madić, V., Jovanović, J., Stojilković, A., Jušković, M., & Vasiljević, P. (2017). Evaluation of cytotoxicity of “anti-diabetic” herbal preparation and five medicinal plants: An Allium cepa assay. Biologica Nyssana, 8(2), 151–158.

Madike, L. N., Takaidza, S., Ssemakalu, C., & Pillay, M. (2019). Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay. South African Journal of Science, 115, 1–6.

Maggi, F., Quassinti, L., Bramucci, M., Lupidi, G., Petrelli, D., Vitali, L. A., Papa, F., & Vittori, S. (2014). Composition and biological activities of hogweed Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt essential oil and its main components octyl acetate and octyl butyrate. Natural Product Research, 28, 1354–1363.

Mazzeo, D. E., Fernandes, T. C., & Marin-Morales, M. A. (2011). Cellular damages in the Allium cepa test system, caused by BTEX mixture prior and after biodegradation process. Chemosphere, 85, 13–18.

Medvedeva, M. Y., Bolsunovsky, A. Y., & Zotina, T. A. (2014). Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River. Contemporary Problems of Ecology, 7(4), 422–432.

Mishyna, M., Laman, N., Prokhorov, V., & Fujii, Y. (2015). Angelicin as the principal allelochemical in Heracleum sosnowskyi fruit. Natural Product Communications, 10(5), 767–770.

Moshafi, M. H., Sharififar, F., Dehghan, G. R., & Ameri, A. (2009). Bioassay screening of the essential oil and various extracts of fruits of Heracleum persicum Desf. and rhizomes of Zingiber officinale Rosc. using brine shrimp cytotoxicity assay. Iranian Journal of Pharmaceutical Research, 8(1), 59–63.

Musikhin, P. V., & Sigaev, А. А. (2006). Issledovanie fizicheskikh svojstv i khimicheskogo sostava borshhevika sosnovskogo i poluchenie iz nego voloknistogo polufabrikata [Investigation of physical properties and chemical composition of Heracleum sosnowskyi and production of fiber semi-product from it]. Sovremennye Naukoemkie Tekhnologii, 3, 65–67 (in Russian).

Musorina, T. А., Naumova, E. А., Shonina, E. V., Petrichenko, M. R., & Kukolev, M. I. (2019). Teplotekhnicheskie svojstva ehnergoehffektivnogo materiala na osnove rastitel’noj dobavki (sukhoj borshhevik) [Heat engineering properties of energy-efficient material based on plant additives (dried hogweed)]. Vestnik MGSU, 14(12), 1555–1571 (in Russian).

Nabeel, M., Abderrahman, S., & Papini, A. (2008). Cytogenetic effects of Arum maculatum extract on the bone marrow cells of mice. Caryologia, 61(4), 383–387.

O‘Hare, S., & Atterwill, C. K. (1995). In vitro toxicity testing protocols. Humana Press, Springer, Heidelberg.

Olorunfemi, D. I., Ogieseri, U. M., & Akinboro, A. (2011). Genotoxicity screening of industrial effluents using onion bulbs (Allium cepa L.). Journal of Applied Sciences and Environmental Management, 15(1), 211–216.

O'Neill, T., Johnson, J. A., Webster, D., & Gray, C. A. (2013). The Canadian medicinal plant Heracleum maximum contains antimycobacterial diynes and furanocoumarins. Journal of Ethnopharmacology, 147(1), 232–237.

Pesnya, D. S., Romanovsky, A. V., Serov, D. A., & Poddubnaya, N. Y. (2017). Genotoxic effects of Heracleum sosnowskyi in the Allium cepa test. Caryologia, 70(1), 55–61.

Prajitha,V., & Thoppil, J. E. (2016). Genotoxic and antigenotoxic potential of the aqueous leaf extracts of Amaranthus spinosus Linn. using Allium cepa assay. South African Journal of Botany, 102, 18–25.

Ribeiro, I. A. (2018). Allium test in enviromental monitoring and healh. Novas Edições Acadêmicas. OmniScriptum Publishing KS, Mauritius.

Serrano-Garcia, L., & Monteiro-Montoya, R. (2001). Micronuclei and chromatid buds are the result of related genotoxic events. Environmental and Molecular Mutagenesis, 38(1), 38–45.

Shakhmatov, E. G., Atukmaev, K. V., & Makarova, E. N. (2016). Structural characteristics of pectic polysaccharides and arabinogalactan proteins from Heracleum sosnowskyi Manden. Carbohydrate Polymers, 136, 1358–1369.

Sharma, S., & Vig, A. P. (2012). Genotoxicity of atrazine, avenoxan, diuron and quizalofop-P-ethyl herbicides using the Allium cepa root chromosomal aberration assay. Terrestrial Aquatic Environmental Toxicology, 6(2), 90–95.

Sharma, S., Panneerselvam, N., & Ma, T. H. (1990). Genetic toxicology of pesticides in higher plant systems. Critical Reviews in Plant Sciences, 9(5), 409–442.

Souri, E., Farsam, H., Sarkheil, P., & Ebadi, F. (2008). Antioxidant activity of some furanocoumarins isolated from Heracleum persicum. Pharmaceutical Biology, 42, 396–399.

Sudhakar, R., Ninge, G. K. N., & Venu, G. (2011). Mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa. Cytologia, 66(3), 235–239.

Sultan, A. O., & Celik, T. A. (2007). Cytotoxic and genotoxic effects of Lavandula stoechas aqueous extracts. Biologia, 62(3), 292–296.

Sultan, A. O., & Celik, T. A. (2010). Evaluation of cytotoxicity and genotoxicity of Inula viscosa leaf extracts with Allium test. Journal of Biomedicine and Biotechnology, 2010, 189252.

Tedesco, S. B., & Laughinghouse IV, H. D. (2012). Bioindicator of genotoxicity: the Allium cepa test. In: Srivastava. J. (Ed.). Environmental Contamination. InTech, Croatia.

Timoshevskij, V. А., & Nazarenko, S. А. (2006). Biologicheskaya indikatsiya mutagennykh vozdejstvij geneticheskoj nestabil’nosti u cheloveka putem ucheta chislovykh khromosomnykh narushenij [Biological indication of the mutagenic influences and genetic instability by aneuploidy detection]. Vestnik Genetiki i selekcii, 10(3), 530–539 (in Russian).

Tkachenko, K. G. (2015). Borshheviki (rod Heracleum (L.): Pro et contra [Giant hogweeds (genus Heracleum (L.): Pro et contra]. Biosfera, 7(2), 209–219 (in Russian).

Trott, J., Gerber, W., Hammes, S., & Ockenfels, H. M. (2008). The effectiveness of PUVA treatment in severe psoriasis is significantly increased by additional UV 308-nm excimer laser sessions. European Journal of Dermatology, 18(1), 55–60.

Ventura-Camargo, B. C., Angelis, D. F., & Marin-Morales, M. A. (2016). Assessment of the cytotoxic, genotoxic and mutagenic effects of the commercial black dye in Allium cepa cells before and after bacterial biodegradation treatment. Chemosphere, 161, 325–332.

Visockiene, J. S., Tumeliene, E., & Maliene, V. (2020). Identification of Heracleum sosnowskyi-invaded land using earth remote sensing data. Sustainability, 12(3), 759.

Walasek, M., Grzegorczyk, A., Malm, A., & Skalicka-Wozniak, K. (2015). Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography. Food Chemistry, 186, 133–138.

Wang, W., Gorsuch, J. W., & Hughes, J. S. (1997). Plants for environmental studies. CRC Lewis Publisher, Boca Raton, New York.

Wierzbicka, M., & Antosiewicz, D. (1988). Allium test-some questions. Acta Societatis Botanicorum Poloniae, 57(2), 201–215.

Yurlova, L. Y., Chernyak, D. M., & Kutovaya, O. P. (2013). Furokumariny Heracleum sosnowskyi i Heracleum moellendorffi [Furocoumarins of Heracleum sosnowskyi and Heracleum moellendorffi]. Tikhookeanskij Meditsinskij Zhurnal, 52, 91–93 (in Russian).

How to Cite
Smirnova, M. V., & Kotelnikov, V. A. (2021). Effect of Heracleum sosnowskyi extract aqueous solution on the Allium cepa root meristem . Regulatory Mechanisms in Biosystems, 12(3), 467-471. https://doi.org/10.15421/022164