Effects of duration and conditions of storage on germination of seeds of Pedicularis sceptrum-carolinum (Orobanchaceae)

  • A. G. Lapirov Papanin Institute for Biology of Inland Waters
  • E. A. Belyakov Papanin Institute for Biology of Inland Waters
  • O. A. Lebedeva Papanin Institute for Biology of Inland Waters
Keywords: rare species; hemiparasites; generative reproduction; germination; Avena sativa; morphological differences.


Choosing optimum conditions for plants of the Pedicularis genus to productively germinate and undergo the initial stages of development is currently a relevant problem in the search of solutions to successfully grow these taxa. For the experiments, seeds of Pedicularis sceptrum-carolinum L. (Lamiales, Orobanchaceae) were collected in the first decade of September in the vicinity of Chashnitskoe Lake. The study of specifics of germination of seeds and the initial stages of the development of P. sceptrum-carolinum was carried out in controlled laboratory conditions in a climate chamber with illumination (1,200–1,500 lux, photoperiod of 9/15, temperature of 23–25 °С). After a month-long storage of seeds in their fruit capsules in the laboratory conditions, the greatest germination (83.3–93.3%) was achieved after their subsequent dry maintenance (taken out of the fruits) in a refrigerator at the temperature of +2…+3 ºС for 3 or 6 months. Lower values of final germination were obtained after maintaining dry seeds at the temperature of –24…–28 ºС for 3 months. Increasing periods of such storage up to six months led to decrease in the final germination and energy of germination. After-ripening lasting different periods provided lower values of the two most important parameters – final germination and energy of germination, even in cases of quite long periods of dry storage in the laboratory. The initial stages of the development of plants from seeds of P. sceptrum-carolinum, which had undergone 3-month stratification in a refrigerator, were studied during the period of 2.0 (2.5) months in different conditions: Petri dishes on moistened filter paper, and in glass vessels with settled tap water, in soil in a plastic container (pure groups of sowed seeds) and also in soil sown together with seeds of Avena sativa L., with seeds put singly into a plastic block of 9 cassettes. The study revealed morphological differences in plants that had developed over the two-months growth, in each variant of the experiment. We recorded fragmentary development of haustorial hairs on the lateral roots of the plants in the pure sown group and also the haustorium in the group sown together with common oat. We achieved no further development and the plants died. The plants grew for a longer period (2.5 months) in the pure sown groups, which then died as well. The study we performed may be a basis for preparing successful introduction and cultivation of P. sceptrum-carolinum, which would be an important source of preservation of a species that raises concerns on account of the rapid decreases in its populations, narrowing of its range and rare occurrence.


Belaeva, T. N., Prokopyev, A. S., Butenkova, A. N., & Astafurova, T. P. (2017). Pollination ecology and seed production of some species of the genus Pedicularis L. in the highlands of Altai. International Journal of Environmental Studies, 74(5), 744–751.

Belyakov, E. A., & Lapirov, A. G. (2015). Fruit germination of some representatives of the family Sparganiaceae Rudolphi under laboratory conditions. Inland Water Biology, 8(1), 33–37.

Bruce, W. R., Gladys, C., Frank, T., & Kilpatrick, S. (2015). Phylogeny and biogeography of North American and Asian Pedicularis (Orobanchaceae). Systematic Botany, 40(1), 229–258.

Cerabolini, B., De Andreis, R., Ceriani, R. M., Pierce, S., & Raimondi, B. (2004). Seed germination and conservation of endangered species from the Italian Alps: Physoplexis comosa and Primula glaucescens. Biological Conservation, 117(3), 351–356.

Copenhaver, M. D., & Holland, B. (1988). Computation of the distribution of the maximum studentized range statistic with application to multiple significance testing of simple effects. Journal of Statistical Computation and Simulation, 30(1), 1–15.

Danylevska, O. M., & Futorna, O. A. (2016). Morfologіchnі osoblyvostі nasіnyn vydіv rodu Pedicularis (Orobanchaceae) flory Ukrajiny [Morphological features of seeds of the Pedicularis (Orobanchaceae) species in the flora of Ukraine]. Ukrainian Botanical Journal, 73(5), 492–502 (in Ukrainian).

Finch-Savage, W. E., & Leubner-Metzge, G. (2006). Seed dormancy and the control of germination. New Phytologist, 171(3), 501–523.

Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2001). PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 4(1), 1–9.

Holub, J., & Procházka, F. (2000). Red List of vascular plants of the Czech Republic – 2000. Preslia, 72, 187–230.

Jensen, K. (2004). Dormancy patterns, germination ecology, and seed-bank types of twenty temperate fen grassland species. Wetlands, 24(1), 152–166.

Kaye, T. N., Liston, A., Love, R. M., Luoma, D. L., Meinke, R. J., & Wilson, M. V. (1997). Seed dormancy in high elevation plants: Implications for ecology and restoration. Conservation and Management of Native Plants and Fungi. In: Proceedings of an Oregon Conference on the Conservation and Management of Native Vascular Plants, Bryophytes, and Fungi. Native Plant Society of Oregon Corvallis. Pp. 115–120.

Kirillova, N. R. (2018). Razvitie Pedicularis palustris L. (Orobanchaceae) na travyanyh bolotah Murmanskoj oblasti [Development of Pedicularis palustris L. (Orobanchaceae) on grassy bogs of the Murmansk region]. Vestnik Murmanskogo Gosudarstvennogo Tekhnicheskogo Universiteta, 21(2), 199–206 (in Russian).

Kirmizi, S., Güleryüz, G., Arslan, H. F., & Sakar, S. (2010). Effects of moist chilling, gibberellic acid, and scarification on seed dormancy in the rare endemic Pedicularis olympica (Scrophulariaceae). Turkish Journal of Botany, 34, 225–232.

Li, A.-R., Guan, K.-Y., & Probert, R. J. (2007). Effects of light, scarification, and gibberellic acid on seed germination of eight Pedicularis species from Yunnan, China. HortScience, 42(5), 1259–1262.

Li, A.-R., Li, Y.-J., Smith, S. E., Smith, F. A., & Guan, K.-Y. (2013). Nutrient requirements differ in two Pedicularis species in the absence of a host plant: Implication for driving forces in the evolution of host preference of root hemiparasitic plants. Annals of Botany, 112(6), 1099–1106.

Liu, M.-L., Yu, W.-B., Li, D.-Z., Mill, R. R., & Hong, W. (2013). Seed morphological diversity of Pedicularis (Orobanchaceae) and its taxonomic significance. Plant Systematics and Evolution, 299(9), 1645–1657.

Maglocký, Š., & Feráková, V. (1993). Red list of ferns and flowering plants (Pteridophyta and Spermatophyta) of the flora of Slovakia (the second draft). Biologia, 48(4), 361–385.

McDonough, W. T. (1970). Germination of 21 species collected from a high-elevation rangeland in Utah. The American Midland Naturalist, 84(2), 551–554.

Nikolaeva, M. G., Lyanguzova, I. V., & Pozdova, L. M. (1999). Biologiya semyan [Biology of Seeds]. Research Institute of Chemistry of Saint-Petersburg State University, Saint-Petersburg (in Russian).

Peregrym, O. M., & Peregrym, M. M. (2014). Poshyrennia vydіv rodu Pedicularis (Orobanchaceae) v Ukrajinі [Distribution of species of the genus Pedicularis (Orobanchaceae) in Ukraine]. Ukrainian Botanical Journal, 71(5), 573–580 (in Ukrainian).

Petrova, S. E., & Pavlenko, E. V. (2017). Biologiya i morfologo-anatomicheskie osobennosti Pedicularis sceptrum-carolinum (Scrophulariaceae) s. l. (Hibiny) [Biology and morphological and anatomical features of Pedicularis sceptrum-carolinum (Scrophulariaceae) s.l. (Khibiny)]. Botanical Journal, 102(4), 526–539 (in Russian).

Petrů, M. (2005). Year-to-year oscillations in demography of the strictly biennial Pedicularis sylvatica and effects of experimental disturbances. Plant Ecology, 181(2), 289–298.

Probert, R. J. (2000). The role of temperature in the regulation of seed dormancy and germination. In: Seeds. The ecology of regeneration in plant communities. CABI International, Wallingford. Pp. 261–292.

Ree, R. H. (2005). Phylogeny and the evolution of floral diversity in Pedicularis (Orobanchaceae). International Journal of Plant Sciences, 166(4), 595–613.

Ren, Y.-Q., & Guan, K.-Y. (2008). Effects of moist-chilling and GA3 applications on seed germination of three Pedicularis species from Yunnan, China. Seed Science and Technology, 36(1), 225–229.

Serap, K., Gürcan, G., Hülya, A., & Selcen, S. F. (2010). Effects of moist chilling, gibberellic acid, and scarification on seed dormancy in the rare endemic Pedicularis olympica (Scrophulariaceae). Turkish Journal of Botany, 34(3), 225–232.

Seregin, A. P. (2011). Pedicularis palustris i P. sceptrum-carolinum (Orobanchaceae) vo Vladimirskoj oblasti i v Srednej Rossii: Dinamika i prichiny vymiraniya [Pedicularis palustris and P. sceptrum-carolinum (Orobanchaceae) in the Vladimir Region and Central Russia: Dynamics and causes of extinction]. Botanical Journal, 96(12), 1561–1574 (in Russian).

Shipley, B., & Parent, M. (1991). Germination responses of 64 wetland species in relation to seed size, minimum time to reproduction and seedling relative growth rate. Functional Ecology, 5(1), 111–118.

Sorokin, A. S. (2016). Mytnik skipetrovidnyj [Pedicularis sceptrum-carolinum L.]. In: Orlov, S. V., Sokolov, D. L., Perova, I. S., Kokina, O. M., Turilov, A. V., & Vedernikova, E. S. (ed.). The Red Book of Tver region. Tver Printing Yard, Tver. P. 123 (in Russian).

Stachurska-Swakon, A., Wołkowycki, D., & Kucharczyk, M. (2014). EN – Pedicularis sceptrum-carolinum L. – Gnidosz królewski. In: Polish Red Data Book of Plants. Pteridophytes and flowering plants. Institute of Nature Conservation Polish Academy of Sciences, Krakow. Pp. 454–456.

Van Assche, J., Van Nerum, D., & Darius, P. (2002). The comparative germination ecology of nine Rumex species. Plant Ecology, 159(2), 131–142.

Voronin, L. V. (2015). Mytnik skipetrovidnyj [Pedicularis sceptrum-carolinum L.]. In: Nyankovsky, M. A. (Ed.). The Red Book of Yaroslavl region. Academy 76, Yaroslavl. P. 198 (in Russian).

Wróblewska, A. (2013). Genetic diversity within populations of Pedicularis sceptrum-carolinum. Annales Botanici Fennici, 50(5), 289–299.

Wróblewska, A., & Mirski, P. (2018). From past to future: impact of climate change on range shifts and genetic diversity patterns of circumboreal plants. Regional Environmental Change, 18(2), 409–424.

How to Cite
Lapirov, A. G., Belyakov, E. A., & Lebedeva, O. A. (2021). Effects of duration and conditions of storage on germination of seeds of Pedicularis sceptrum-carolinum (Orobanchaceae) . Regulatory Mechanisms in Biosystems, 12(2), 234-239. https://doi.org/10.15421/022132