Microscopic changes in the organs of broiler chickens with Ornithobacterium rhinotra-cheale infection

  • B. V. Borysevych National University of Life and Environmental Sciences of Ukraine
  • V. B. Dukhnytskyj National University of Life and Environmental Sciences of Ukraine
  • A. M. Tyshkivska National University of Life and Environmental Sciences of Ukraine
  • M. Y. Tyshkivsky Bila Tserkva National Agrarian University
  • N. V. Tyshkivska Bila Tserkva National Agrarian University
Keywords: ornithobacteriosis; respiratory diseases; broilers; microscopic changes; histological examinations; bacteriological research; sensitivity to antibiotics; Maldi Tof


Nowadays ornithobacteriosis is widespread among industrial poultry in Ukraine and the world. The disease leads to significant economic losses. The large variation in the serotypes of the bacteria complicates the treatment of the disease. The study of microscopic changes that occur in organs during ornithobacteriosis will help to better understand the pathogenesis of the disease development. Ornithobacteriosis was diagnosed in clinically sick broiler chickens by bacteriological studies using Maldi Tof mass-spectrometry. As a result of the antibiotic sensitivity test, the pathogen was found to be sensitive to doxycycline, tilmicosin, rifampicin, cefazolin, amoxiclav and benzylpenicillin. Histological studies of internal organs – trachea, lungs, heart, kidneys, liver and spleen – were carried out. It was found that with spontaneous ornithobacteriosis of broiler chickens, the most expressive microscopic changes occur in the respiratory organs – the lungs and trachea and are characterized by a decrease in the lumen of the parabronchials due to edema of their walls and infiltration with lymphocytes, the absence of epithelium on the surface of the parabronchials, narrowing of the air capillaries of the parabronchial complexes, hemorrhages in the parenchyma of the parabronchial complexes, vasodilatation of the lung vessels and overflow of their blood vessels with blood cells, edema around the epithelium trachea, destruction of part of the tracheal mucosa epithelial cells, edema of its submucosa, expansion and overflow of blood vessels of the mucous membrane with blood cells. In the spleen there was a uniform diffuse edema of the parenchyma and a decrease in the number of lymphocytes in the lymphoid follicles; in the kidneys – expansion and overflow of the stroma blood vessels, uneven edema of the glomeruli of one part of the renal corpuscles and destruction of the glomeruli of another, granular degeneration of the convoluted and straight tubules of the kidneys; in the liver – edema, hemorrhage, violation of the hepatic lobules’ structure, expansion of the hepatic veins, granular degeneration of hepatocytes or their destruction; in the heart – edema of the myocardial interstitium, muscle fibers’ granular dystrophy, fragmentation of muscle fibers as a result of their rupture. Histological research of ORT infected chickens will lead to a better understanding the mechanism of pathological changes at the microscopic level, which will facilitate the development of more effective methods of treatment and prevention of the disease.


Barbosa, E. V., Cardoso, C. V., Silva, R., Cerqueira, A., Liberal, M., & Castro, H. (2020). Ornithobacterium rhinotracheale: An update review about an emerging poultry pathogen. Veterinary Science, 7(1), 1–13.

Chu, J., Zhang, Q., Zuo, Z. J., El-Ashram, S., Guo, Y., Zhao, P., Huang, S., He, C., & Khan, A. (2017). Coinfection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens. Scientific Reports, 7(1), 1–10.

Ellakany, H. F., Elbestawy, A. R., Abd-Elhamid, H. S., Gado, A. R., Nassar, A. A., Abdel-Latif, M., Ghanima, I., El-Hack, M., Swelum, A., Saadeldin, I., Ba-Awadh, H., & Alowaimer, A. (2019). Effect of experimental Ornithobacterium rhinotracheale infection along with live infectious bronchitis vaccination in broiler chickens. Poultry Science, 98(1), 105–111.

Gavrilović, P., Gavrilović, A., Vidanović, D., Parunović, J., & Jovanović, M. (2015). Comparative pathomorphological, bacteriological and serological examination of broiler breeders and pheasants experimentally infected with Ornithobacterium rhinotracheale. Avian Pathology, 45(5), 513–519.

Hauck, R., Chin, R. P., & Shivaprasad, H. L. (2015). Retrospective study on the isolation of Ornithobacterium rhinotracheale from chickens and turkeys in central California: 294 cases (2000-12). Avian Diseases, 59(1), 130–137.

Kapakin, K., Büyük, F., Sahin, M., & Kapakin, S. (2013). Respiratory tract infection induced experimentally by Ornithobacterium rhinotracheale in quails: Effects on heat shock proteins and apoptosis. Revue de Médecine Vétérinaire, 164(3), 132–140.

Kempf, I., Reeve-Johnson, L., & Gesbert, F. (1997). Efficacy of tilmicosin in the control of experimental Mycoplasma gallisepticum infection in chickens. Avian Diseases, 4, 802–807.

Kilic, A., Timurkaan, N., Ertaş, A. A., & Yilmaz, F. (2009). Pathological examination and bacterial re-isolation by culture and PCR of experimental Ornithobacterium rhinotracheale infection in broiler chickens. Revue de Médecine Vétérinaire, 160(3), 140–144.

Kotsyumbas, G. I., & Blishch, G. I. (2015). Histopathological changes in chickens lund of Ornithobacterium rhinotracheale. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, Series Veterinary sciences, 61, 78–82.

Kotsyumbas, G. I., & Blishch, G. I. (2015). Histopathological changes in chicken-broilers spleen of Ornithobacterium rhinotracheale. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, Series Veterinary Sciences, 63, 66–70.

Kotsyumbas, G. I., Blishch, G. I., & Shchebentovska, O. M. (2015). Pathological changes in chickens of Ornithobacterium rhinotracheale. Veterinary Medicine, 100, 149–152.

Mayahi, M., Gharibi, D., Ghadimipour, R., & Talazadeh, F. (2016). Isolation, identification and antimicrobial sensitivity of Ornithobacterium rhinotracheale in broilers chicken flocks of Khuzestan, Iran. Veterinary Research Forum, 7(4), 341–346.

Morales-Erasto, V., Falconi-Agapito, F., Luna-Galaz, G. A., Saravia, L., Montalvan-Avalos, A., Soriano-Vargas, E., & Fernández-Díaz, M. (2016). Coinfection of Avibacterium paragallinarum and Ornithobacterium rhinotracheale in chick-ens from Peru. Avian Diseases, 60(1), 75–78.

Oca-Jimenez, R. M., Vega-Sanchez, V., Morales-Erasto, V., Salgado-Miranda, C., Blackall, P., & Soriano-Vargas, E. (2018). Phylogenetic relationship of Ornithobacterium rhinotracheale strains. The Journal of Veterinary Medical Science, 80(6), 869–873.

Rosa-Ramos, M., Muñoz-Solís, K., Palma-Zepeda, M., Gutierrez-Castillo, A., Villegas, E., Guerra-Infante, F., & Castro-Escarpulli, G. (2018). Adherence of Ornithobacterium rhinotracheale to chicken embryo lung cells as a pathogenic mechanism. Avian Pathology, 47(2), 172–178.

Szabó, R., Wehmann, E., Makrai, L., Nemes, C., Gyuris, É., Thuma, Á., & Magyar, T. (2017). Characterization of Ornithobacterium rhinotracheale field isolates from Hungary. Avian Pathology, 46(5), 506–514.

Wafaa, A., & El-Ghany, A. (2009). The in-vitro and in-vivo evaluation of tiamulin and tilmicosin for the treatment of Mycoplasma gallisepticum infected broiler chickens. International Journal of Poultry Science, 8(12), 1189–1198.

Xue, J., Lv, C., He, P., & Zhang, G. (2020). Serological investigation of Ornithobacterium rhinotracheale infection in China. Poultry Science, 99, 10.

How to Cite
Borysevych, B. V., Dukhnytskyj, V. B., Tyshkivska, A. M., Tyshkivsky, M. Y., & Tyshkivska, N. V. (2021). Microscopic changes in the organs of broiler chickens with Ornithobacterium rhinotra-cheale infection . Regulatory Mechanisms in Biosystems, 12(1), 27-32. https://doi.org/10.15421/022105