Differential species traits of Trichostrongylus tenuis (Nematoda, Trichostrongylidae)

  • V. O. Yevstafieva Poltava State Agrarian Academy
  • Y. S. Starodub Poltava State Agrarian Academy
  • V. M. Pisarenko Poltava State Agrarian Academy
  • O. V. Barabolia Poltava State Agrarian Academy
  • O. V. Nikiforova Kharkiv State Zooveterinary Academy
Keywords: trichostrongylosis; goose; nematodes; helminth eggs; differential diagnostics.


Trichostrongylus tenuis Mehlin, 1846 is a helminth species that parasitizes in the gastrointestinal tract of birds and causes trichostrongylosis. Research on the differential features of the pathogen at various stages of development facilitates timely finding of the foci of infection and providing recommendations for prevention and control. In the present study, the differential species traits of male and female Trichostrongylus nematodes parasitizing in the domestic goose were examined. The nematodes were obtained in helminthological dissection of the intestine of birds kept at farms and private households in Poltava region, Ukraine. It was revealed that the morphological features of T. tenuis females that should be considered in species identification include the structural specifics of the reproductive system and the tail end. 22 metric parameters were suggested for species identification, including the female body measurements for different parts, specifics of vulva and anus location, sizes of the ovijector, sphincter, ejector and vulval area. Differences were found in the metric parameters of the lateral crests of the cuticle in female nematodes in relation to the position of crests on the parasite’s bodies. The cuticular crests were absent at 0.1 mm from the head and tail ends, and the length of crests was 9.3 mm in total. The longest and widest crests were observed in the middle of the body, the shortest were found in the anus area, and the narrowest crests were from the anterior part of esophagus to its middle. The distance between crests varied 0.2–3.0 μm, the longest at the transition of esophagus into the intestine and the shortest in area between the vulva and the anus. The metric parameters of T. tenuis eggs obtained from bird feces and in the nematode uterus differed significantly by 5 characters. The collected data on the morphological and metric parameters of females and eggs of T. tenuis can be used in species identification and understanding of the taxonomic position of that species.


Afolabi, O. J., Simon-Oke, I. A., & Olasunkanmi, A. O. (2016). Intestinal parasites of domestic chicken (Gallus gallus domesticus) in Akure, Nigeria. Journal of Biomedical Science, 1(4), e9771.

Andrade, J. G., Kumsa, B., Ayana, D., Vieira, R., Santos, C. P., Iñiguez, A. M., & DaMatta, R. A. (2018). First record of the nematode Libyostrongylus dentatus Hoberg, Lloyd & Omar, 1995 (Trichostrongylidae) in ostriches (Struthio camelus Linnaeus, 1758) (Struthionidae) outside the Americas. Parasites and Vectors, 11(1), 243.

Araújo, A., Jansen, A. M., Bouchet, F., Reinhard, K., & Ferreira, L. F. (2003). Parasitism, the diversity of life, and paleoparasitology. Memorias do Instituto Oswaldo Cruz, 98(1), 5–11.

Atkinson, C. T., Thomas, N. J., & Hunter, D. B. (2008). Parasitic diseases of wild birds. Wiley-Blackwell, Ames, Iowa.

Bonadiman, S. F., Ederli, N. B., Soares, A. K., de Moraes Neto, A. H., Santos, C., & DaMatta, R. A. (2006). Occurrence of Libyostrongylus sp. (Nematoda) in ostriches (Struthio camelus Linnaeus, 1758) from the north region of the state of Rio de Janeiro. Brazilian Journal of Veterinary Parasitology, 137, 175–179.

Boyko, A. A., & Brygadyrenko, V. V. (2016). Influence of water infusion of medicinal plants on larvae of Strongyloides papillosus (Nematoda, Strongyloididae). Visnyk of Dnipropetrovsk University, Biology, Ecology, 24(2), 519–525.

Boyko, O. O., & Brygadyrenko, V. V. (2019). The impact of acids approved for use in foods on the vitality of Haemonchus contortus and Strongyloides papillosus (Nematoda) larvae. Helminthologia, 56(3), 202–210.

Carlson, C. J., Burgio, K. R., Dougherty, E. R., Phillips, A. J., Bueno, V. M., Clements, C. F., Castaldo, G., Dallas, T. A., Cizauskas, C. A., Cumming, G. S., Doña, J., Harris, N. C., Jovani, R., Mironov, S., Muellerklein, O. C., Proctor, H. C., & Getz, W. M. (2017). Parasite biodiversity faces extinction and redistribution in a changing climate. Science Advances, 3(9), e1602422.

Cervantes-Rivera, К., Villagómez-Cortés, J. А., Arroyo-Lara, А., & Landín-Grandvallet, L. A. (2016). A diagnostic survey of gastroenteric helminths in backyard poultry of a rural village in Mexican tropics. Journal of Agricultural and Biological Science, 11(12), 463–469.

Connan, R. M., & Wise, D. R. (1993). Development and survival at low temperature of the free living stages of Trichostrongylus tenuis. Research in Veterinary Science, 55(1), 20–24.

Cram, E., & Cuvillier, E. (1934). Observations on Trichostrongylus tenuis infestation in domestic and game birds in the United States. Parasitology, 26(3), 340–345.

Cram, E., & Wehr, E. (1934). The status of species of Trichostrongylus of birds. Parasitology, 26(3), 335–339.

Davidson, W. R., Kellogg, F. E., Doster, G. L., & Moore, C. T. (1991). Ecology of helminth parasitism in bobwhites from northern Florida. Journal of Wildlife Diseases, 27(2), 185–205.

De Jong, Y., Verbeek, M., Michelsen, V., Bjørn, P., de P., Los, W., Steeman, F., Bailly, N., Basire, C., Chylarecki, P., Stloukal, E., Hagedorn, G., Wetzel, F. T., Glöckler, F., Kroupa, A., Korb, G., Hoffmann, A., Häuser, C., Kohlbecker, A., Müller, A., Güntsch, A., Stoev, P., & Penev, L. (2014) Fauna Europaea – all European animal species on the web. Biodiversity Data Journal, 2, e4034.

Durette-Desset, M. C. (1985). Trichostrongyloid nematodes and their vertebrate hosts: Reconstruction of the phylogeny of a parasitic group. Advances in Parasitology, 24, 239–306.

Durette-Desset, M. C. (1992). Phylogeny of Trichostrongyloidea nematodes as seen through some of their vertebrate hosts. Parassitologia, 34, 1–16.

Durette-Desset, M. C., Chabaud, A. G., & Moore, J. (1993). Trichostrongylus cramae n. sp. (Nematoda), a parasite of bob-white quail (Colinus virginianus). Annales de Parasitologie, 68(1), 43–48.

Ederli, N. B., & Oliveira, F. C. (2014). Comparative morphology of the species of Libyostrongylus and Codiostomum, parasites from ostriches, Struthio camelus, with a identification key to the species. Revista Brasileira de Parasitologia Veterinaria, 23(3), 291–300.

Enigk, K., & Dey-Hazra, A. (1971). Propagation and treatment of the Trichostrongylus tenuis infestations. Berliner und Munchener tierarztliche Wochenschrift, 84(1), 11–14.

Ewald, P. W. (1995). The evolution of virulence: A unifying link between parasitology and ecology. Journal of Parasitology, 81(5), 659–669.

Giannetto, S. (2006). Biomorphology of gastrointestinal nematodes of small ruminants. Parassitologia, 48(3), 391–395.

Gibbons, L. M., & Khalil, L. F. (1982). A key for the identification of genera of the nematode family Trichostrongylidae Leiper, 1912. Journal of Helminthology, 56(3), 185–233.

Hoberg, E. P. (1996). Emended description of Mazamastrongylus peruvianus (Nematoda: Trichostrongylidae), with comments on the relationships of the genera Mazamastrongylus and Spiculopteragia. Journal of Parasitology, 82(3), 470–477.

Hoberg, E. P., & Lichtenfels, J. R. (1994). Phylogenetic systematic analysis of the Trichostrongylidae (Nematoda), with an initial assessment of coevolution and biogeography. Journal of Parasitology, 80(6), 976–996.

Hudson, P. J. (1986). The effect of a parasite nematode on the breeding production of red grouse. Journal of Animal Ecology, 55, 85–94.

Irungu, L. W., Kimani, R. N., & Kisia, S. M. (2004). Helminth parasites in the intestinal tract of indigenous poultry in parts of Kenya. Journal of the South African Veterinary Association, 75(1), 58–59.

Jacquiet, P., Cabaret, J., Cheikh, D., & Thiam, E. (1997). Identification of Haemonchus species in domestic ruminants based on morphometrics of spicules. Parasitology Research, 83(1), 82–86.

Kennedy, M. M., & Harnett, W. (2013). Parasitic nematodes: Molecular biology, biochemistry and immunology. CABI, United Kingdom.

Lee, D. L. (1965). The cuticle of adult Nippostrongylus brasiliensis. Parasitology, 55, 173–181.

Lichtenfels, J. R., & Pilitt, P. A. (2000). Synlophe patterns of the Haemonchinae of ruminants (Nematoda: Trichostrongyloidea). Journal of Parasitology, 86(5), 1093–1098.

Lichtenfels, J. R., Hoberg, E. P., Pilitt, P. A., & Craig, T. M. (1996). The synlophe and other structural characteristics of Sarwaria bubalis (Nematoda: Trichostrongyloidea) from cattle in Guyana. Journal of Parasitology, 82(1), 146–154.

Mukaratirwa, S., Cindzi, Z. M., & Maononga, D. B. (2004). Prevalence of Libyostrongylus douglassii in commercially reared ostriches in the highveld region of Zimbabwe. Journal of Helminthology, 78(4), 333–336.

O'connor, L. J., Walkden-Brown, S. W., & Kahn, L. P. (2006). Ecology of the free-living stages of major trichostrongylid parasites of sheep. Veterinary Parasitology, 142, 1–15.

Pesenti, T. C., Gallina, T., Langone, P. Q., Silva, M. A., Suárez, A., Silva, D. S., & Müller, G. (2015). Infecção por nematóides em Struthio camelus (Linnaeus, 1758) (avestruz) no sul do Brasil. Science of Animal Health, 3(1), 103–112.

Ryzhikov, К. М. (1967). Opredelitel’ gel’mintov domashnih vodoplavajushhih ptic [Key to the helminths of domestic water birds]. Nauka, Moscow (in Russian).

Rzayev, F. H., Seyidbeyli, M. I., Maharramov, S. H., & Gasimov, E. K. (2020). Formy i ul’trastrukturnye osobennosti lateral’nyh kryl’ev gel’minta Trichostrongylus tenuis Mehlis, 1846 (Nematoda: Trichostrongylidae) [Forms and ultrastructural features of the lateral alae of the helminth Trichostrongylus tenuis Mehlis, 1846 (Nematoda: Trichostrongylidae)]. Journal of V. N. Karazin Kharkiv National University, 34, 112–119 (in Russian).

Shaw, J. L., & Moss, R. (1990). Effects of the caecal nematode Trichostrongylus tenuis on egg-laying by captive red grouse. Research in Veterinary Science, 48(1), 59–63.

Skrjabin, K. I. (1928). Metod polnyh gel’mintologicheskih vskrytij pozvonochnyh, vkljuchaja cheloveka [The method of complete helminthological autopsy of vertebrates, including humans]. Moscow State University, Moscow (in Russian).

Skrjabin, K. I., Shikhobalova, N. P., & Shults, R. S. (1954). Osnovy nematodologii. Trihostrongilidy zhivotnyh i cheloveka [Essentials of nematology. Trichostrongylids of animals and man]. Nauka, Moscow (in Russian).

Souza, R. P., Souza, J. N., Menezes, J. F., Alcântara, L. M., Soares, N. M., & Aquino Teixeira, M. C. (2013). Human infection by Trichostrongylus spp. in residents of urban areas of Salvador city, Bahia, Brazil. Biomedica, 33(3), 439–445.

Sudhaus, W., & Fitch, D. (2001). Comparative studies on the phylogeny and systematics of the Rhabditidae (Nematoda). Journal of Nematology, 33(1), 1–70.

Sukee, T., Beveridge, I., & Jabbar, A. (2018). Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp. Parasites and Vectors, 11(1), 271.

Tuggle, B. N., & Crites, J. L. (1984). The prevalence and pathogenicity of gizzard nematodes of the genera Amidostomum and Epomidiostomum (Trichostrongylidae) in the lesser snow goose (Chen caerulescens caerulescens). Canadian Journal of Zoology, 62(9), 1849–1852.

Wascher, C. A. F., Bauer, A. C., Holtmann, A. R., & Kotrschal, К. (2012). Environmental and social factors affecting the excretion of intestinal parasite eggs in graylag geese. Behavioral Ecology, 23(6), 1276–1283.

Woog, F., Maierhofer, J., & Haag, Н. (2011). Endoparasites in the annual cycle of feral greylags Anser anser. Wildfowl, 61, 164–179.

Yevstafieva, V. А., Stybel, V. V., Melnychuk, V. V., Prijma, О. В., Yatsenko, I. V., Antipov, A. A., Bakhur, T. I., Goncharenko, V. P., Pidborska, R. V., Shahanenko, V. S., & Dzhmil, V. I. (2019). Morphological and biological characteristics of Amidostomum anseris (Nematoda, Amidostomatidae) from Anser anser domesticus. Vestnik Zoologii, 53(1), 65–74.

Yevstafieva, V., & Starodub, Y. (2020). Poshyrennja tryhostrongil’ozu gusej na terytoriji Poltavs’koji oblasti [Distribution of trichostrongylosis of geese on the territory of Poltava region]. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 97, 125–129 (in Ukrainian).

Yoshino, T., Uemura, J., Endoh, D., Kaneko, M., Osa, Y., & Asakawa, M. (2009). Parasitic nematodes of anseriform birds in Hokkaido, Japan. Helminthologia, 46(2), 117–122.

Zelmer, D. A. (1998). An evolutionary definition of parasitism. International Journal for Parasitology, 28(3), 531–533.

How to Cite
Yevstafieva, V. O., Starodub, Y. S., Pisarenko, V. M., Barabolia, O. V., & Nikiforova, O. V. (2020). Differential species traits of Trichostrongylus tenuis (Nematoda, Trichostrongylidae) . Regulatory Mechanisms in Biosystems, 11(3), 449–454. https://doi.org/10.15421/022069