Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan)
AbstractA total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantitative traits were noted as per IPGRI, 1994 descriptor. Among the quantitative parameters, a high level of genetic variability index was noted in seeds per spike at 79.9% of coefficient of variance followed by biomass per plant which shows 37.4% variance, while minimum variance in quantitative traits was noted in days to germination at 5.4% followed by days to maturity at 3.1% with average mean genetic variation in all quantitative traits at 97.6%. Assay of total seed protein in these exotic accessions was analogue through polyacrylamide gel electrophoresis. A high level of variation was noted in loci (bands) B26 (0.98%) followed by B25 (0.89%), B24 (0.78%),B23 (0.69%) and B01 (0.52%). A similarly low level of variation was detected in B03 (0.16%) followed by B06 (0.18%), B13 (0.19%), B12 (0.21%), B11 (0.23%), B05 (0.24%), B07 (0.25%), B21 (0.34%), B20 (0.35%), B17 (0.39%). The results indicate that the mean value of variation in these accessions is 97.6%. Further assessments and exploration were suggested for these genotypes in multi-climatic zones to satisfy farmers’ need, breeders’ interest and malt-industrial requirements.
Baik, B.-K., & Ullrich, S. E. (2008). Barley for food: Characteristics, improvement, and renewed interest. Journal of Cereal Science, 48(2), 233–242.
Baloch, A. W., Ali, M., Baloch, A. M., Mangan, B.-U.-N., & Song, W. (2014). Genetic diversity and structure analysis based on hordein protein polymorphism in barley landrace populations from Jordan. Pakistan Journal of Botany, 46(4), 1397–1402.
Derbew, S., Mohammed, H., & Urage, E. (2013). Phenotypic diversity for qualitative characters of barley (Hordeum vulgare L.) landrace collections from Southern Ethiopia. International Journal of Science and Research, 2(9), 34–40.
Ebrahim, S., Shiferaw, E., & Hailu, F. (2015). Evaluation of genetic diversity in barley (Hordeum vulgare L.) from Wollo high land areas using agromorphological traits and hordein. African Journal of Biotechnology, 14(22), 1886–1896.
Ferreira, J. R., Pereira, J. F., Turchetto, C., Minella, E., Consoli, L., & Delatorre, C. A. (2016). Assessment of genetic diversity in Brazilian barley using SSR markers. Genetics and Molecular Biology, 39(1), 86–96.
Hassan, N., Wadood, S., Ullah, H., Zahoor, M., Khan, M., Khan, A., & Nisar, M. (2016). Estimation of genetic diversity in Cupressus sempervirens growing in different ecological zones of Malakand Division, KP, Pakistan. International Journal of Biosciences, 8, 15–21.
Hua, W., Zhang, X., Zhu, J., Shang, Y., Wang, J., Jia, Q., Li, C., & Yang, J. (2014). A study of genetic diversity of colored barley (Hordeum vulgare L.) using SSR markers. Genetic Resources and Crop Evolution, 62(3), 395–406.
Ivandic, V., Thomas, W. T. B., Nevo, E., Zhang, Z., & Forster, B. P. (2003). Associations of simple sequence repeats with quantitative trait variation including biotic and abiotic stress tolerance in Hordeum spontaneum. Plant Breeding, 122(4), 300–304.
Khodayari, H., Saeidi, H., Roofigar, A. A., Rahiminejad, M. R., Pourkheirandish, M., & Komatsuda, T. (2012). Genetic diversity of cultivated barley landraces in Iran measured using microsatellites. International Journal of Bioscience, Biochemistry and Bioinformatics, 2, 287–290.
Ladizinsky, G., & Hymowitz, T. (1979). Seed protein electrophoresis in taxonomic and evolutionary studies. Theoretical and Applied Genetics, 54(4), 145–151.
Ledovskoy, Y, Abugalieva, S., & Turuspekov, Y. (2010). Comparative assessment of the genetic variation in wild and cultivated barley based on SSR markers. Asian and Australasian Journal of Plant Science and Biotechnology, 4, 21–26.
Mzid, R., Chibani, F., Ayed, R. B., Hanana, M., Breidi, J., Kabalan, R., El-Hajj, S., Machlab, H., Rebai, A., & Chalak, L. (2016). Genetic diversity in barley landraces (Hordeum vulgare L. subsp. vulgare) originated from Crescent Fertile region as detected by seed storage proteins. Journal of Genetics, 95(3), 733–739.
Nandha, P. S., & Singh, J. (2013). Comparative assessment of genetic diversity between wild and cultivated barley using gSSR and EST-SSR markers. Plant Breeding, 133(1), 28–35.
Sipahi, H., Akar, T., Yıldız, M. A., & Sayım, İ. (2010). Determination of genetic variation and relationship in Turkish barley cultivars by hordein and RAPD markers. Turkish Journal of Field Crops, 15(2), 108–113.
Toivonen, M., Herzon, I., & Helenius, J. (2013). Environmental fallows as a new policy tool to safeguard farmland biodiversity in Finland. Biological Conservation, 159, 355–366.
Tsen, C. C. (1985). Amino acid composition and biological value of cereal germs. In: Amino acid composition and biological value of cereal proteins, Springer. Pp. 453–466.
Vapa, L., & Radović, D. (1998). Genetics and molecular biology of barley hordeins. Cereal Research Communications, 26, 31–38.
Weber, B., Collins, C., Robbins, C., Magenis, R. E., Delaney, A. D., Gray, J. W., & Hayden, M. R. (1990). Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n. Nucleic Acids Research, 18(11), 3353–3361.
Yahiaoui, S., Igartua, E., Moralejo, M., Ramsay, L., Molina-Cano, J. L., Ciudad, F. J., Lasa, J. M., Gracia, M. P., & Casas, A. M. (2007). Patterns of genetic and eco-geographical diversity in Spanish barleys. Theoretical and Applied Genetics, 116(2), 271–282.
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