Metabolic processes in the organism of animals under the action of plant extract

  • O. Buchko Institute of Animal Biology, National Academy of Sciences of Ukraine
  • V. Havryliak Lviv Polytechnic National University
  • O. Yaremkevych Lviv Polytechnic National University
  • R. Konechna Lviv Polytechnic National University
  • N. Ohorodnyk Lviv National Agrarian University
Keywords: Urtica dioica L.; nettle extract; adrenalin stress; rat; antioxidant system; free radical processes.

Abstract

The possibility of increasing metabolism and resistance in an organism of animals under the influence of biologically active substances of natural origin is considered in the paper. In the current study, we investigated the effect of 40% ethanolic extract of common nettle (Urtica dioica L.) on hematologic indexes (hemoglobin concentration, erythrocytes and leukocytes content), protein metabolism (total protein, aspartate aminotransferase (AST), alanine aminotransferase activities (ALT)), glutathione metabolism (peroxidase (GP), glutathione reductase (GR) and reduced glutathione content (GSH)), antioxidant defense systems (superoxide dismutase (SOD), catalase (CAT)), and indicators of free radical processes (lipid hydroperoxidation products (LHP), TBA-active products and carbonyl groups of protein (CP)) in the blood and tissues of normal and adrenalin-stressed rats. White Wistar rats (male) with body weight 180–200 g were used in the experiments. The animals were divided into 4 groups (control and 3 experimental), each containing 7 animals. The animals of experimental groups D2 and D3 received 40% ethanolic extract of common nettle in a dose of 5 mL/kg of body weight during 4 weeks. After 29 days of the experiment, the animals of D1 and D3 groups were intramuscularly administered 0.1% solution of adrenaline hydrochloride at a dose of 1 mg/kg of body weight. One day after the administration of adrenaline, the animals were decapitated under ether anesthesia. The objects of the study were blood and homogenates of liver, heart, and kidneys of the rats. Our results have shown that adrenaline stress causes a decrease in the concentration of hemoglobin, LHP, SOD activity and increase in activities of AST, GP, GR, and GSH content in the blood of rats. Stress induced by adrenaline was accompanied by decrease in CP content, activities of SOD, CAT, and increase in the glutathione chain of antioxidant defense system in the liver of the animals while in the heart and kidneys, there was an increase in oxidative stress parameters and decrease in antioxidant enzyme activities (SOD, CAT, GP, GR). The administration of ethanolic extract of nettle caused an increase within the physiological range in the erythrocytes and leucocytes content, hemoglobin concentration, and normalization of protein metabolism (decrease in the activity of AST and increase in protein concentration) in the blood of the stressed rats. The activation of the antioxidant defense system (increase in activities of SOD, GP, CAT, GR) and inhibition of free radical processes (decrease in concentration of LHP, TBA-active products and CP) was observed in the tissues of animals of D2 and D3 groups. Our results suggested that using a complex of biologically active substances of the common nettle ethanolic extract prevents metabolic disorders and stresses of different etiology through the activation of the antioxidant defense system, the normalization of protein metabolism and stimulation of hematopoiesis both in normal and stressed rats.

References

Abuajah, C. I., Ogbonna, A. C., & Osuji, C. M. (2015). Functional components and medicinal properties of food: A review. Journal of Food Science and Technology, 52(5), 2522–2529.

Ahmed, A. A., Zain, U., Abjuluziz, M. A., Rius, U., Iubul, H., & Muhammad, T. (2012). Evaluation of the chemical composition and element analysis of Urtica dioica. African Journal of Pharmacy and Pharmacology, 21, 1555–1558.

Ahn, K. (2017). The worldwide trend of using botanical drugs and strategies for developing global drugs. Biochemistry and Molecular Biology Reports, 50(3), 111–116.

Birben, E., Sahiner, U. M., Sackesen, C., Erzurum, S., & Kalayci, O. (2012). Oxidative stress and antioxidant defense. World Allergy Organization Journal, 5(1), 9–19.

Buchko, O., Iaremkevych, O., & Konechna, R. (2016). Antyoksydantna aktyvnist' kropyvy dvodomnoi' (Urtica dioica L.) [Antioxidant activity of nettle (Urtica dioica L.)]. Visnyk of the Lviv University, Biology, 73, 3–7 (in Ukrainian).

Cai, Z., & Yan, L. J. (2013). Protein oxidative modifications: Beneficial roles in disease and health. Journal of Biochemical and Pharmacological Research, 1(1), 15–26.

Carr, A. C., & Lykkesfeldt, J. (Eds.). (2017). Vitamin C in health and disease. Nutrients, MDPI.

Chendey, T. V., Rishko, M. V., & Boyko, N. V. (2013). Vplyv spozhyvannja kropyvy na endotelial'nu funkciju ta markery sercevo-sudynnogo ryzyku v grupah vysokogo ryzyku: Randomizovane kontrol'ovane doslidzhennja [Effects of consumption of nettle on endothelial function and cardiovascular risk markers in at-risk groups: A randomized controlled trial]. Scientific Bulletin of the Uzhgorod University, Medicine, 46, 51–56 (in Ukrainian).

Choi, D. Y., Lee, Y. J., & Hong, J. T. (2012). Antioxidant properties of natural polyphenols and their therapeutic potentials for Alzheimer’s disease. Brain Research Bulletin, 87(2–3), 144–153.

De Vico, G., Guida, V., & Carella, F. (2018). Urtica dioica (Stinging nettle): A neglected plant with emerging growth promoter/immunostimulant properties for farmed fish. Frontiers in Physiology, 285, 1–5.

Gaballu, F. A., Gaballu, Y. A., Khyavy, O. M., Mardomi, A., Ghahremanzadeh, K., Shokouhi, B., & Mamandy, H. (2015). Effects of a triplex mixture of Peganum harmala, Rhus coriaria, and Urtica dioica aqueous extracts on metabolic and histological parameters in diabetic rats. Pharmaceutical Biology, 53(8), 1104–1109.

Ghasemi, S., Moradzadeh, M., Hosseini, M., Beheshti, F., & Sadeghnia, H. R. (2018). Beneficial effects of Urtica dioica on scopolamine-induced memory impairment in rats: Protection against acetylcholinesterase activity and neuronal oxidative damage. Drug and Chemical Toxicology, 10, 1–9.

Gorovaya, N. Y., Plaksen, N. V., & Ustinova, L. V. (2015). Podtverzhdenie effektivnosti i bezopasnosti zhidkogo ekstrakta iz list'ev eleuterokokka [Proof of the efficacy and safety of the liquid extract from the leaves of Eleutherococcus]. Pacific Medical Journal, 2, 66–68 (in Russian).

Güler, E. R. (2013). Investigation of chemopreventive properties of Urtica dioica L., in MCF-7 and MDA 231 breast cancer cell lines. The New England Journal of Medicine, 30, 50–53.

Gupta, R. K., Patel, A. K., & Kumar, R. (2012). Interactions between oxidative stress, lipid profile and antioxidants in breast cancer: A case control study. Asian Pacific Journal of Cancer Prevention, 13(12), 6295–6298.

Gupta, R. K., Patel, A. K., Shah, N., Choudhary, K. A., Jha, U. K., Yadav, U. C., Gupta, P. K., & Pakuwal, U. (2014). Oxidative stress and antioxidants in disease and cancer: A review. Asian Pacific Journal of Cancer Prevention, 15(11), 4405–4409.

Hea, L., Hea, T., Farrarb, S., Ji, L., Liua, T., & Ma, X. (2017). Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cellular Physiology and Biochemistry, 44, 532–553.

Jakubczyk, K., Janda, K., Szkyrpan, S., Gutowska, I., & Wolska, J. (2015). Stinging nettle (Urtica dioica L.) – botanical characteristics, biochemical composition and health benefits. Pomeranian Journal of Life Sciences, 61(2), 191–198.

Johnson, T. A., Sohn, J., Inman, W. D., Bjeldanes, L. F., & Rayburn, K. (2013). Lipophilic stinging nettle extracts possess potent antiinflammatory activity, are not cytotoxic and may be superior to traditional tinctures for treating inflammatory disorders. Phytomedicine, 20(2), 143–147.

Joshi, B. C., Mukhija, M., & Kalia, A. N. (2014). Pharmacognostical review of Urtica dioica L. International Journal of Green Pharmacy, 8(4), 201–209.

Joshi, B. C., Prakash, A., & Kalia, A. N. (2015). Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl4 challenged rats. Toxicology Reports, 2, 1101–1110.

Juma, K. K., Maina, S. G., Muriithi, J. N., Mwangi, B. M., Mworia, J. K., Mwonjoria, M. J., Ngeranwa, J. J. N., & Mburu, D. N. (2015). Protective effects of Urtica dioica and cimetidine on liver function following acetaminophen induced hepatotoxicity in mice. Journal of Developing Drugs, 4(2), 1–7.

Kaledaite, R., & Bernatoniene, J. (2011). Investigation of antiradical activity of Salvia officinalis L., Urtica dioica L., and Thymus vulgaris L. extracts as potential candidates for a complex therapeutic preparation. Journal of Medicinal Plants Research, 5(25), 6090–6096.

Kandis, H., Karapolat, S., Yildirim, U., Saritas, A., Gezer, S., & Memisogullari, R. (2010). Effects of Urtica dioica on hepatic ischemia-reperfusion injury in rats. Clinics (Sao Paulo), 65(12), 1357–1361.

Khariv, М. (2016). Dynamika aktyvnosti aminotransferaz syrovatky krovi shhuriv za oksydacijnogo stresu ta dii' liposomal'nogo preparatu [Dynamics of indices of aminotransferase activity in the blood serum of rats under conditions of oxidative stress and effect of liposomal medicinal product]. Visnyk of Dnipropetrovsk University, Biology, Medicine, 7(1), 3–7 (in Ukrainian).

Khavrona, O. (2015). Porushennja funkcionuvannja glutationovoi' lanky antyoksydantnogo zahystu v slyzovij obolonci shlunka, pechinci ta erytrocytah shhuriv pry eksperymental'nij vyrazkovij hvorobi [Disturbances of function of the glutathione antioxidant system in gastric mucosa, liver and erythrocytes of rats with experimental gastric lesions]. Experimental and Clinical Physiology and Biochemistry, 69(1), 26–31 (in Ukrainian).

Kopyt’ko, Y. F., Lapinskaya, E. S., & Sokol’skaya, T. A. (2011). Primenenie, himicheskij sostav i standartizacija syr'ja i preparatov Urtica (Obzor) [Application, chemical composition, and standardization of nettle raw material and related drugs]. Chemical-Pharmaceutical Journal, 45(10), 32–41 (in Russian).

Lindblad, M., Tveden-Nyborg, P., & Lykkesfeldt, J. (2013). Regulation of vitamin C homeostasis during deficiency. Nutrients, 5(8), 2860–2879.

Mirtaghi, S. M., Torbati Nejad, P. T., Mazandarani, M., Livani, F., & Bagheri, H. (2016). Evaluation of antibacterial activity of Urtica dioica L. leaf ethanolic extract using agar well diffusion and disc diffusion methods. Medical Laboratory Journal, 10(5), 15–21.

Mizutani, T., & Masaki, H. (2014). Anti-photoaging capability of antioxidant extract from Camellia japonica leaf. Experimental Dermatology, 23(1), 23–26.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.

Namazi, F., Shomali, T., Taghikhani, P., & Nazifi, S. (2018). Protective effect of Urtica dioica leaf hydro alcoholic extract against experimentally-induced atherosclerosis in rats. Avicenna Journal of Phytomedicine, 8(3), 254–262.

Oltjen, J. W., Kebreab, E., & Lapierre, H. (2013). Energy and protein metabolism and nutrition in sustainable animal production. EAAP Scientific Series, 134.

Paidi, M. D., Schjoldager, J. G., Lykkesfeldt, J., & Tveden-Nyborg, P. (2014). Prenatal vitamin C deficiency results in differential levels of oxidative stress during late gestation in foetal guinea pig brains. Redox Biology, 2, 361–367.

Qaid, A. (2016). Role of insulin and other related hormones in energy metabolism – A review. Cogent Food and Agriculture, 2, 1–18.

Rabasa, C., & Dickson, S. L. (2016). Impact of stress on metabolism and energy balance. Current Opinion in Behavioral Sciences, 9, 71–77.

Rutto, L. K., Xu, Y., Ramirez, E., & Brandt, M. (2013). Mineral properties and dietary value of raw and processed stinging nettle (Urtica dioica L.). International Journal of Food Science, 2013, 1–9.

Salih, N. A. (2015). Effect of nettle (Urtica dioica) extract on gentamicin induced nephrotoxicity in male rabbits. Asian Pacific Journal of Tropical Biomedicine, 5(9), 756–760.

Seliya, M., & Kothiyal, P. (2014). Urtica dioica (stinging nettle): A review of its chemical, pharmacological, toxicological and ethnomedical properties. International Journal of Pharmacy, 4(1), 270–277.

Shkurashivska, S. V., & Erstenyuk, H. M. (2015). Dynamika zmin pokaznykiv lipidnogo obminu v organah i tkanynah eksperymental'nyh tvaryn za umov adrenalinovogo stresu [Dynamics of changes in lipid metabolism indices in organs and tissues of experimental animals under adrenaline stress]. Medical and Clinical Chemistry, 17(4), 34–37 (in Ukrainian).

Spatafora, C., & Tringali, C. (2012). Natural-derived polyphenols as potential anticancer agents. Anti-Cancer Agents in Medicinal Chemistry, 12(8), 902–918.

Teloa, S., Halifeoglub, I., & Ozercan, I. H. (2017). Effects of stinging nettle (Urtica dioica L.) on antioxidant enzyme activities in rat model of mammary gland cancer. Iranian Journal of Pharmaceutical Research, 16, 164–170.

Vlizlo, V. V. (Ed.). (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory methods of investigation in biology, stock-breeding and veterinary]. Spolom, Lviv (in Ukrainian).

Wolska, J., Janda, K., Szkyrpan, S., & Gutowska, I. (2015). The influence of stinging nettle (Urtica dioica L.) extracts on the activity of catalase in THP1 monocytes/macrophages. Pomeranian Journal of Life Sciences, 61(3), 315–318.

Woo, Y., Lee, H., Jeong, Y.-S., Shin, G. Y., Oh, J. G., Kim, J.-S., & Oh, J. (2017). Antioxidant potential of selected Korean edible plant extracts. BioMed Research International, 17, 1–9.

Yakimova, T. V., Nasanova, O. N., & Vengerovsky, A. I. (2015). Vlijanie ekstraktov lekarstvennyh rastenij na metabolicheskie narushenija pri modeli saharnogo diabeta i insulinorezistentnosti [Influence of herbal extracts on metabolic disturbances in diabetes mellitus and insulin resistance model]. Bulletin of Siberian Medicine, 14(2), 75–81 (in Russian).

Yang, E. J., Lee, J.-S., Song, B. B., Yun, C.-Y., Kim, D.-H., & Kim, I. S. (2011). Anti-inflammatory effects of ethanolic extract from Lagerstroemia indica on airway inflammation in mice. Journal of Ethnopharmacology, 136(3), 422–427.

Zagayko, A. L., Krasilnikova, O. A., Kravchenko, G. B., & Kochubey, Y. I. (2017). Vyvchennja gepatoprotektornoi' aktyvnosti roslynnyh polifenoliv na modeli eksperymental'noi' insulinorezystentnosti [Hepatoprotective activity study of plant polyphenols under the experimental insulin resistance]. World of Medicine and Biology, 59, 117–121 (in Ukrainian).

Zemmouri, H., Sekioua, O., Ammar, S., Feki, A. E., Bouaziz, M., Messaraha, M., & Boumendjel, A. (2017). Urtica dioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model. Pharmaceutical Biology, 55(1), 1561–1568.

Zhang, Y.-J., Gan, R.-Y., & Lietal, S. (2015). Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules, 20(12), 21138–21156.

Published
2019-05-19
How to Cite
Buchko, O., Havryliak, V., Yaremkevych, O., Konechna, R., & Ohorodnyk, N. (2019). Metabolic processes in the organism of animals under the action of plant extract . Regulatory Mechanisms in Biosystems, 10(2), 149-158. https://doi.org/10.15421/021922