Analysis of the genetic structure of a population of Lebedyn cattle by microsatellite markers

Keywords: polymorphism; microsatellites; genotyping; Lebedyn breed; gene pool.

Abstract

Microsatellites – a separate class of molecular genetic markers, are widely used for the study of genetic variability, in particular in populations of animals bred by artificial selection under the influence of microevolutionary processes. The object of study is the gene pool of a population of animals of the Lebedyn cattle breed, which is under threat of extinction. The sample comprised 30 individuals from the farm "Komyshans'ke" in Sumy region. The analysis of population genetic structure was performed using 10 microsatellite loci recommended by FAO-ISAG: ETH225, BM2113, ETH3, BM1818, BM1824, ILSTS006, INRA023, TAGLA053, TAGLA12, ETH10. Amplification products were separated in polyacrylamide gels of different concentrations (5–8%), both native and denaturing. All studied loci were polymorphic. The number of detected alleles per locus ranged from 4 to 8 (on average 5 alleles per locus), the size of which ranged from 115 bp (ETH3) to 307 bp (ILSTS006). The majority of the investigated loci (except ETH3) belonged to valuable informative markers (PIC > 0.5). The most polymorphic TGLA053 (8 alleles), BM2113 (6) and ETH3 (6) loci have been identified. In general, the minimum number of alleles (4) was fixed in 50% loci. The main population genetic parameters for the studied loci have been calculated. The highest values of heterozygosity (He), and effective number of alleles (ne) was characterized for loci BM2113, ILSTS006, TGLA053 and ETH225. With the exception of ETH3 and VM1818 loci, the experimental group of animals is in a state of genetic equilibrium. The average value of the Wright fixation index indicates a tendency to increase in the number of homozygous individuals (inbreeding). Comparative analysis of genetic structure of breeds that have a common origin (Lebedyn (PJSC "Mykhaylivka"), Ukrainian grey (DPDG "Polyvanivka"), Red steppe (DPPR "Stepne"), etc.) has been carried out. The obtained results give grounds to assert that in the experimental population of the Lebedyn breed there are processes that lead to a decrease in genetic diversity. In order to overcome the negative effects of artificial reproduction in the gene pool of small populations of cattle, which include Lebedyn cattle, it is appropriate to use microsatellite markers in the selection and breeding work.

References

Al-Samarai, F. R., & Al-Kazaz, A. A. (2015). Molecular markers and its applica tions in animal breeding: A review. American Journal of Applied Scientific Research, 1(1), 1–5.

Baranovs'kyy, D. I., Herasymov, V. I., & Nahayevych, V. M. (2005). Henofond sviys'kykh tvaryn Ukrayiny [The gene pool of domestic animals of Ukraine]. Espada, Kharkiv (in Ukrainian).

Basovs'kyy, D. M. (2013). Problemy zberezhennya henofondu lebedyns'koyi po rody [Problems of preserving the gene pool of Lebedyn cattle]. Rozvedennya i henetyka tvaryn. Animal Breeding and Genetics, 47, 145–151 (in Ukrainian).

Debrauwere, H., Gendrel, C. G., Lechat, S., & Dutreix, M. (1997). Differences and similarities various tandem repeat sequences: Minisatellites and microsa tellites. Biochimie, 79, 577–586.

FAO (2011). Molecular genetic characterization of animal genetic resources. FAO animal production and health guidelines. No. 9. Rome, Italy.

Glazko, V. I. (2011). Genomnaya selektsiya krupnogo rogatogo skota: issledova tel'skie i prikladnye zadachi [Genomic selection of cattle: Research and applied tasks]. Izvestiya TSKhA, 5, 126–135 (in Russian).

Guseev, Y. V., Мtlyyk, О. V., Gladyr, E. A., & Zinovieva, N. A. (2016). The polymorphism of five microsatellite DNA loci in the study of grey Ukrainian and Bulgarian grey cattle breeds. Animal Breeding and Genetics, 52, 202–211.

Hladiy, M. V., Polupan, Y. P., Basovs'kyy, D. M., & Vyshnevs'kyy, L. V. (2018). Prohrama zberezhennya henofondu lokal'nykh i znykayuchykh porid sil's'ko hospodars'kykh tvaryn v Ukrayini na 2017–2025 [Program for preserving the gene pool of local and endangered breeds of farm animals in Ukraine for 2017–2025]. Sumy (in Ukrainian).

Khedrik, F. (2003). Genetika populyatsiy [Genetics of populations]. Tekhnosfera, Moscow (in Russian).

Khlestkina, E. K. (2014). Molecular markers in genetic studies and breeding. Russian Journal of Genetics: Applied Research, 4(3), 236–244.

Kramarenko, A. S., Gladyr, E. A., Kramarenko, S. S., Pidpala, T. V., Strikha, L. A., & Zinovieva, N. A. (2018). Genetic diversity and bottleneck analysis of the Red Steppe cattle based on microsatellite markers. Ukrainian Journal of Ecology, 8(2), 12–17.

Kulibaba, R. A., & Liashenko, Y. V. (2016). Influence of the PCR artifacts on the genotyping efficiency by the microsatellite loci using native polyacrylamide gel electrophoresis. Cytology and Genetics, 50(3), 162–167.

Kuznetsov, V. M. (2014). F-statistiki Rayta: Otsenka i interpretatsiya [Wright's F-statistics: Evaluation and interpretation). Problems of Biology of Productive Animals, 4, 80–104 (in Russian).

Merkur'eva, E. K. (1977). Geneticheskie osnovy selektsii v skotovodstve [Genetic principles of selective breeding in cattle breeding]. Kolos, Moscow (in Russian).

Mishra, S. P., Mishra, C., Mishra, D. P., Rosalin, B. P., & Bhuyan, C. (2017). Application of advanced molecular marker technique for improvement of animal: A critical review. Journal of Entomology and Zoology Studies, 5(5), 1283–1295.

Naqvi, A. N. (2007). Application of molecular genetic technologies in livestock production: Potentials for developing countries. Advances in Biological Research, 1(3–4), 72–84.

Prokhorenko, P. (2013). Golshtinskaya poroda i ee vliyanie na geneticheskiy progress produktivnosti cherno-pestrogo skota evropeyskikh stran i rossiy skoy federatsii [Holstein breed and its influence on the genetic progress of the productivity of Black-and-White cattle in European countries and the Russian Federation]. Molochnoe i Myasnoe Skotovodstvo, 2, 2–6 (in Russian).

Schmid, M., Saitbekova, N., Gaillard, C., & Dolf, G. (1999). Genetic diversity in Swiss cattle breeds. Journal of Animal Breeding and Genetics, 116, 1–8.

Senan, S., Kizhakayil, D., Sasikumar, B., & Sheeja, T. E. (2014). Methods for development of microsatellite markers: An overview. Notulae Scientia Biologicae, 6(1), 1–13.

Shel'ov, A. V. (2015). Polimorfizm mikrosatelitnykh lokusiv DNK u riznykh vydiv sil's'kohospodars'kykh tvaryn [Polymorphism of microsatellite DNA loci in different species of farm animals]. Animal Breeding and Genetics, 50, 183–190 (in Ukrainian).

Shel'ov, A. V., Kopylov, K. V., Kramarenko, S. S., & Kramarenko, O. S. (2017). Analysis of population-genetic processes in different cattle breeds by micro satellite loci of DNA. Agricultural Science and Practice, 4(1), 74–78.

Shkavro, N. M., Radko, A., Slota, E., & Rossokha, V. I. (2010). Polimorfizm mikrosatelitnykh markeriv DNK dvokh porid velykoyi rohatoyi khudoby [Polymorphism of microsatellite DNA markers two breeds of cattle]. Visnyk Kharkivs'koho Natsional'noho Universytetu imeni V. N. Karazina. Seriya Biolohiya, 905(11), 120–126 (in Ukrainian).

Shkavro, N., Blyzniuk, O., Pomitun, I., & Babicz, M. (2018). Evaluation of the genetic structure and main productive traits of Lebedyn cattle based on genetic markers polymorphism. Journal of Animal Science Biology and Bioeconomy, 36(2), 17–26.

Shuplyk, V. V., Savchuk, O. V., & Huzyev, I. V. (2013). Henofond porid sil's'ko hospodars'kykh tvaryn Ukrayiny [Genetic pool of breeds of farm animals in Ukraine]. Vydavets' PP Zvoleyko D. H., Kam’yanets’-Podil’s’kyy (in Ukrainian).

Sirats'kyy, Y. Z. (Ed.). (2001). Bura khudoba v Ukrayini [Brown Cattle in Ukraine]. Naukovyy Svit, Kyiv (in Ukrainian).

Stolpovskiy, Y. A., & Zakharov-Gezekhus, I. A. (2017). Problema sokhraneniya genofondov domestitsirovannykh zhivotnykh [The problem of preserving the gene pools of domesticated animals]. Vavilov Bulletin of Genetics and Breeding, 21(4), 477–486 (in Ukrainian).

Sulimova, G. E. (2004). DNK-markery v geneticheskikh issledovaniyakh: Tipy markerov, ikh svoystva i oblasti primeneniya [DNA markers in genetic studies: Types of markers, their properties and areas of application]. Succes ses of Modern Biology, 124, 260–271 (in Russian).

Teneva, A., Todorovska, E., Tyufekchiev, N., Atanassov, A., Foteva, S., Ralcheva, S., & Zlatarev, S. (2005). Molecular characterization of Bulgarian livestock genetic resources [Genetic diversity in Bulgarian grey cattle as revealed by microsatellite markers]. Biotechnology in Animal Husbandry, 21(5–6), 35–41.

Tisdell, C. (2003). Socioeconomic causes of loss of animal genetic diversity: Analysis and assessment. Ecological Economics, 45(3), 365–376.

Yatsenko, A. E. (1997). Lebedinskaya poroda krupnogo rogatogo skota [Lebedyn breed of cattle]. BMT, Kyiv (in Russian).

Published
2019-02-23
How to Cite
LadykaV. І., Khmelnychyi, L. M., Lyashenko, Y. V., & Kulibaba, R. O. (2019). Analysis of the genetic structure of a population of Lebedyn cattle by microsatellite markers . Regulatory Mechanisms in Biosystems, 10(1), 45-49. https://doi.org/10.15421/021907