Influence of solution of lactoprotein with sorbitol on ultrastructural changes in lungs of rats in the condition of burn shock


Keywords: thermal damage; skin; infusion therapy; pulmonary injuries

Abstract

This article gives a theoretical substantiation and a new experimental solution of a scientific problem aimed at increasing the effectiveness of pharmacotherapy on the morphofunctional state of the lungs of rats under conditions of burn shock by using a combined colloid-hyperosmolar infusion solution – lacto-protein with sorbitol. The administration of the test solution at a dose of 10 ml/kg for 7 days in rats with modelled burn shock reduced ultrastructural changes in the lungs triggered by burn shock. It has been proved that in the conditions of shock, colloid-hyperosmolar infusion lacto-protein with sorbitol solution facilitates the restoration of vascular endothelium and fluid retention in the microcirculatory channel and improves the morphofunctional state of the aerohematic barrier of the lungs, stimulates the activity of the alveolar macrophages and the secretory function of the type II alveolocytes producing surfactant. At day 7 of burn shock, when 0.9% of NaCl was injected, significant changes were observed in the respiratory unit: part of the alveoli had considerably enhanced clearance of blood capillaries, which had platelets, neutrophils and altered forms of erythrocytes. At day 7 of burn shock in the lungs of the rats given an infusion of colloid-hyperosmolar solution – lactoprotein with sorbitol, the ultrastructure of the components of the lung cells had improved in comparison with 3 days. Luminosity of the hemocapillary parts was moderate, mainly with erythrocytes. The walls of endothelial cells had elongated nuclei with invaginations of nuclear membranes and clear contours. Their cytoplasmic regions were not widespread, with moderate electron densities. In type II alveolocytes, during this experiment, a lower degree of damage to the nucleus and organelles in the cytoplasm was established, and there were signs of a renewal of the secretory function of these cells. In the cytoplasm, hypertrophied mitochondria with clear crystals, different sizes of secretory granules, which had a different density, indicating their formation, were observed. According to the magnitude of the cytoprotective effect on lung cells under conditions of burn shock, the lactoprotein with sorbitol solution was shown to be superior in comparison with the physical solution (0.9% NaCl). The study of functional, biochemical and molecular genetic parameters that characterize the state of the aerohematic barrier under the conditions of using lactoprotein with sorbitol solution in the case of burn injuries of the skin will allow researchers to comprehensively evaluate the mechanisms of the pulmonary protective effect of this preparation and to experimentally substantiate the expediency of its use in clinical practice for pharmaco-correction of burn shock.

References

Abdullahi, A., & Jeschke, M. G. (2014). Nutrition and anabolic pharmacotherapies in the care of burn patients. Nutrition in Clinical Practice, 29(5), 621–630.


Abdullahi, A., Amini-Nik, S., & Jeschke, M. (2014). Animal models in burn research. Cellular and Molecular Life Sciences, 71(17), 3241–3255.


Cherkasov, V. G., Kovalchuk, А. I., Dzevulskaya, I. V., & Cherkasov, E. V. (2015). Evaluation of the effect of infusion of composite hyperosmolar solutions on the structure of the neuroimmunoendocrine system organs in burn disease. European International Journal of Science and Technology, 4(9), 51–61.


Chong, S. J., Wong, Y. C., Wu, J., Tan, M. H., Lu, J., & Moochhala, S. M. (2014). Parecoxib reduces systemic inflammation and acute lung injury in burned animals with delayed fluid resuscitation. International Journal of Inflammation, 2014, 972645.


Cox, R. A., Jacob, S., Andersen, C. R., Mlcak, R., Sousse, L., Zhu, Y., & Hawkins, H. K. (2015). Integrity of airway epithelium in pediatric burn autopsies: Association with age and extent of burn injury. Burns, 41(7), 1435–1441.


Directive 2010/63/EC of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (2010). Official Journal of the European Union, 276, 33–79.


Dong, Z. W., Chen, J., Ruan, Y. C., Zhou, T., Chen, Y., Chen, Y., & Peng, Y. Z. (2015). CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury. Scientific Reports, 5, 15946.


Fuzaylov, G., Anderson, R., Knittel, J., & Driscoll, D. N. (2015) Global health: Burn outreach program. Journal of Burn Care and Research, 36(2), 306–309.


Gamelli, L., Mykychack, I., Kushnir, A., Driscoll, D. N., & Fuzaylov, G. (2015) Targeting burn prevention in Ukraine: Evaluation of base knowledge in burn prevention and first aid treatment. Journal of Burn Care and Research, 36(1), 225–231.


Glik, J., Kawecki, M., Kitala, D., Klama-Baryła, A., Łabuś, W., Grabowski, M., & Kasperczyk, A. (2017). A new option for definitive burn wound closure-pair matching type of retrospective case-control study of hand burns in the hospitalised patients group in the Dr Stanislaw Sakiel Centre for Burn Treatment between 2009 and 2015. International Wound Journal, 14(5), 849–855.


Guminskiy, Y. I., Gunas, I. V., Ocheretna, N. P., & Bashinska, O. I. (2017). Micromorphometric changes in rats’ spleen in the first 7 days after skin burns and under application of infusion solutions. Reports of Morphology, 23(2), 240–244.


Guo, S.-X., Fang, Q., You, C.-G., Jin, Y.-Y., Wang, X.-G., Hu, X.-L., & Han, C.-M. (2015). Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation. Journal of Translational Medicine, 13, 183.


Herold, S., Gabrielli, N. M., & Vadász, I. (2013). Novel concepts of acute lung injury and alveolar-capillary barrier dysfunction. American Journal of Physiology – Lung Cellular and Molecular Physiology, 305(10), L665–L681.


Jacob, S., Zhu, Y., Kraft, R., Cotto, C., Carmical, J. R., Wood, T. G., & Cox, R. A. (2015). Physiologic and molecular changes in the tracheal epithelium of rats following burn injury. International Journal of Burns and Trauma, 5(1), 36–45.


Kaddoura, I., Abu-Sittah, G., Ibrahim, A., Karamanoukian, R., & Papazian, N. (2017). Burn injury: Review of pathophysiology and therapeutic modalities in major burns. Annals of Burns and Fire Disasters, 30(2), 95–102.


Nielson, C. B., Duethman, N. C., Howard, J. M., Moncure, M., & Wood, J. G. (2017). Burns: Pathophysiology of systemic complications and current management. Journal of Burn Care and Research, 38(1), e469–e481.


Perel, P., Roberts, I., & Ker, K. (2013). Colloids versus crystalloids for fluid resuscitation in critically ill patients. Cochrane Database of Systematic Reviews, 2(2), CD000567.


Porter, C., Tompkins, R. G., Finnerty, C. C., Sidossis, L. S., Suman, O. E., & Herndon, D. N. (2016). The metabolic stress response to burn trauma: Current understanding and therapies. Lancet, 388(10052), 1417–1426.


Regas, F. C., & Ehrlich, H. P. (1992). Elucidating the vascular response to burns with a new rat model. The Journal of Trauma, 32(5), 557–563.


Rose, L. F., & Chan, R. K. (2016). The burn wound microenvironment. Advances in Wound Care, 5(3), 106–118.


Rowan, M. P., Cancio, L. C., Elster, E. A., Burmeister, D. M., Rose, L. F., Natesan, S., & Chung, K. K. (2015). Burn wound healing and treatment: Review and advancements. Critical Care, 19, 243.


Shaver, C. M., & Bastarache, J. A. (2014). Clinical and biological heterogeneity in acute respiratory distress syndrome: Direct versus indirect lung injury. Clinics in Chest Medicine, 35(4), 639–653.


Snell, J. A., Loh, N.-H. W., Mahambrey, T., & Shokrollahi, K. (2013). Clinical review: The critical care management of the burn patient. Critical Care, 17(5), 241.


Sousse, L. E., Herndon, D. N., Andersen, C. R., Zovath, A., Finnerty, C. C., Mlcak, R. P., Cox, R. A., Traber, D. L., & Hawkins, H. K. (2015). Pulmonary histopathologic abnormalities and predictor variables in autopsies of burned pediatric patients. Burns, 41(3), 519–527.


Zhang, D., Chang, Y., Han, S., Yang, L., Hu, Q., Yu, Y., & Chai, J. (2018). The microRNA expression profile in rat lung tissue early after burn injury. Turkish Journal of Trauma and Emergency Surgery, 24(3), 191–198.

Published
2018-08-25
How to Cite
Ocheretnyuk, A. O., Palamarchuk, O. V., Lysenko, D. A., Vashchuk, G. I., & Stepanyuk, G. I. (2018). Influence of solution of lactoprotein with sorbitol on ultrastructural changes in lungs of rats in the condition of burn shock. Regulatory Mechanisms in Biosystems, 9(3), 440-445. https://doi.org/https://doi.org/10.15421/021866

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