Bile acids from bile of rats of different sexes under testosterone
Abstract
Among the various functions of the liver, the formation of bile plays an important role. The optimal physiological ratio of bile components and the content of testosterone in the blood depend on various factors that can cause biliary system dysfunction and secretion. In experiments on different-sex rats, changes in bile acid contents of bile under the influence of testosterone propionate, which was injected intramuscularly 0.7 mg/kg, for 5 days were investigated. With the method of thin-layer chromatography, the basic fractions of bile acids conjugated in the bile were defined – taurocholic, taurochenodeoxycholic and taurodeoxycholic, glycocholic, glycochenodeoxycholic and glycodeoxycholic and free – cholic, chenodeoxycholic and deoxycholic acids. Conjugation rates were calculated (the ratio of the sum of conjugated cholates to the amount of free ones) and hydroxylation (ratio of the sum of trihydroxycholate bile acids to the sum of dihydroxycholanic) bile acids. In the bile of female rats almost all concentrations of cholates increased, except glycochenodeoxycholic and glycodeoxycholic acids. The calculated conjugation index on the whole did not undergo significant changes, but the hydroxylation factor increased, which may indicate an intensification of bile acid biosynthesis by neutral means, which is realized by 7α-hydroxylation of cholesterol. Under the influence of the hormone in male rats, the content of conjugated bile acids increased, and as for the free ones – a multidirectional effect of testosterone is observed, in particular, the concentration of cholic acid significantly decreased, indicating the activation of the poly-enzyme systems providing its conjugation with glycine and taurine. In connection with the wide use of the drug testosterone propionate and in view of its identified effects on the bile acid contents of the course of intramuscular administration, it is advisable to investigate the effect of this drug on the productive capacity of the liver.References
Atamnah, S., Reshetnik, J., Levadjans'ka, J., Baranovs'kyj, V., Vesel's'kyj, S., & Janchuk, P. (2015). Vplyv serotoninu na kon’jugaciju ta gidroksyljuvannja zhovchnyh kyslot u pechinci shhuriv [Serotonin effects on conjugation and hydroxylation of bile acid in the rats liver]. Naukovyj Visnyk Shidno-Jevropejs'kogo Universytetu Imeni Lesi Ukrai'nky, 2, 124–127 (in Ukranian).
Borovec', O., Bened', V., Reshetnik, J., Vesel's'kyj, S., & Makarchuk, M. (2016). Zhovchnosekretorna funkcija pechinky samok shhuriv v umovah blokady estrogenovyh receptoriv tamoksyfenom [Bile secretion liver function in the female rats at estrogen receptor tamoxifen blockade conditions]. Naukovyj Visnyk Shidno-Jevropejs'kogo Universytetu Imeni Lesi Ukrai'nky, 7, 194–199 (in Ukranian).
Boyer, J. L. (2013). Bile formation and secretion. Comprehensive Physiology, 3(3), 1035–1078.
Gerbina, N. A., Ruban, О. A., Kutsenko, S. A., & Hontovaya, T. N. (2017). Perspektyvy zastosuvannja likars'koi' roslynnoi' syrovyny ta efirnyh olij pry patologijah biliarnogo traktu [Prospects of using medicinal herbs and essential oils for the treatment of the biliary tract]. Chasopys Fitoterapija, 1, 8–16 (in Ukranian).
Klymjuk, O., Bondzyk, O., Vesel's'kyj, S., & Makarchuk, M. (2010). Vplyv estronu na zovnishn'osekretornu funkciju pechinky shhuriv [Influence of estrone on the external secretion function of the liver of rats]. Visnyk Kyyivsʹkoho Natsionalʹnoho Universytetu Imeni Tarasa Shevchenka, 56, 59–61 (in Ukranian).
Levadjans'ka, J., Reshetnik, J., Vesel's'kyj, S., & Janchuk, P. (2017). Zhovchnokyslotnyj spektr zhovchi samciv shhuriv pry dii' L-cystei'nu [Spectrum of bile acids in the bile of male rats after the administration of L-cysteine]. Naukovyj Visnyk Shidno-Jevropejs'kogo Universytetu Imeni Lesi Ukrai'nky, 7, 221–226 (in Ukranian).
Reshetnik, J. (2012). Vplyv blokady opioidnyh receptoriv na spivvidnoshennja holativ u zhovchi shhuriv iz eksperymentalnymalkogolnym gepatytom [Effect of opioid receptor blockade on the relation of cholates in bile of rats with experimental alcoholic hepatitis]. Tavrycheskyj Medyko-Byologycheskyj Vestnіk, 15(3), 280–282 (in Ukranian).
Rozen, V. B., Mataradze, G. D., Smyrnova, O. B., & Smyrnov, A. N. (1991). Polovaja dyfferencyrovka funkcyj pecheny [Gender differentiation of liver function]. Medycyna, Moscow (in Russian).
Synel'nyk, T. B., Synel'nyk, O. D., & Rybal'chenko, V. K. (2003). Zhovchni kysloty v procesah utvorennja kanal'cevoi' zhovchi [Bile acids the processes of canalicular bile formation]. Fiziologichnyj Zhurnal, 49(6), 80–93 (in Ukranian).
Tomych, M. I. (2015). Docil'nist' vykorystannja gepatoprotektoriv u kompleksnomu likuvanni dobrojakisnyh dysgormonal'nyh dysplazij molochnyh zaloz [Advisability of use of hepatoprotectors is in the treatment dishormonal dysplasia оf breast]. Zdorov'e Zhenshhyny, 98, 101–103 (in Ukranian).
Vesel's'kyy, S. P., Lyashchenko, P. S., & Luk'janenko, Y. A. (1991). Sposob opredelenyja zhelchnyyh kyslot v byologycheskyh zhydkostjah [A method of determining bile acids in biological fluids]. Avtorskoe Svydetel'stvo 1624322 (in Ukranian).
Yin, L. J., & Wang, X. H. (2018). Research advance in the effects of androgen and its receptor on the development of obesity, obesity-related diseases and disorders of glucose and lipid metabolism. Sheng Li Xue Bao, 70(3), 319–328.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons «Attribution» 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.