Biochemical and hematological composition of blood of cattle fed with Chlorella

A. A. Bogdanova; E. A. Flerova

doi:10.15421/021836

A. A. Bogdanova Yaroslavl Research Institute of Livestock and Fodder Production – Affiliate of “FWRC FPA” http://orcid.org/0000-0002-6636-9287
E. A. Flerova The Yaroslavl Demidov State University http://orcid.org/0000-0002-9745-6746

Keywords: lactating cows; heifers; bioreactor; electrostatic field; fodder additive; leukogram

Abstract

We researched the influence of the additive Chlorella cultivated in a closed bioreactor under the influence of an electrostatistic field, on the biochemical and hematological parameters of blood in different age groups of cattle. The experiment was conducted on two groups of three month old heifers and two groups of lactating cows in the 7th month of lactation, with 15 individuals in each group; all animals in the groups were given the basic diet. During the 120-day experiment, the experimental group of heifers was fed on a suspension of Chlorella cultivated with the technology using an electrostatic field, in the following amounts: 1–30th day – 1.51 g, 31–60th day – 1.82 g, 61–90th day – 2.01 g, 91–120th day – 2.28 g of dry substance per individual daily; the group of lactating cows during 60 days was given the Chlorella additive to the amount of 6 g of dry substance per individual per day. Over the following 30 days the heifers and lactating cows were kept under observation to determine the “aftereffects” of the Chlorella suspension fodder additive. On the first day of the experiment and after every 30 days, blood was drawn from the animals’ jugular veins for determination of the biochemical and hematological parameters of the blood During the experiment, the experimental groups of heifers and lactating cows were observed to have a strengthened antioxidant system due to increase in the activity of superoxide dismutase and catalase, and also to have a strengthened hematopoietic function and intensified metabolic and redox processes. Due to the stimulating effect of the suspension of Chlorella cultivated using the intense technology involving using an electrostatic field on the erythro- and leukopoiesis of different age groups of the cattle, we determined a strengthening of the cellular element of the animals’ immune system. During the following 30 days, the heifers and lactating cows of the experimental groups were observed to exhibit “aftereffects” of the Chlorella suspension additive, namely increases in the values of biochemical, hematological and morphological indicators of the blood in comparison with these indicators for the animals in the control groups. As a result, we concluded that feeding different age groups of cattle with suspension of Chlorella cultivated in the conditions of a closed bioreactor under the influence of an electrostatic field, in the dosage of 155 ml per kg of dry substance of the animals’ diet, contributes to the strengthening of non-specific defence of their organism, forming a potential for increasing the productive indicators and maintenance of the livestock.

References

Aliahmat, N. S., Noor, M. R. M., Yusof, W. J. W., Makpol, S., Ngah, W. Z. W., & Yusof, Y. A. M. (2012). Antioxidant enzyme activity and malondialdehyde levels can be modulated by Piper betle, tocotrienol rich fraction and Chlorella vulgaris in aging C57BL/6 mice. Clinics, 67(12), 1447–1454.

Becker, E. W. (2007). Micro-algae as a source of protein. Biotechnology Advances, 25(2), 207–210.

Bishop, W. M., & Zubeck, H. M. (2012). Evaluation of microalgae for use as nutraceuticals and nutritional supplements. Journal of Nutrition and Food Sciences, 2(5), 1–6.

Bogdanov, N. I. (2004). Ispol'zovanie chlorelly v racione sel'skohozjajstvennyh zhivotnyh [Use of Chlorella in the diet of farm animals]. Rossijskaja Sel'skohozjajstvennaja Nauka, 1, 34–35 (in Russian).

Bogdanova, A. A., & Flerova, E. A. (2016). Vlijanie dobavki chlorelly na fiziologo-biohimicheskie i produktivnye pokazateli u telochek [Effect of chlorella on physiological, biochemical parameters and productive traits in heifers]. Problemy Biologii Produktivnyh Zhivotnyh, 2, 66–75 (in Russian).

Borowitzka, M. A. (1999). Commercial production of microalgae: Ponds, tanks, tubes and fermenters. Journal of Biotechnology, 70(1–3), 313–321.

Carvalho, A. P., Meireles, L. A., & Malcata, F. X. (2006). Microalgal reactors: A review of enclosed system designs and performances. Biotechnology Progress, 22(6), 1490–1506.

Chevari, S., CHaba, I., & Sekej, J. (1985). Rol' superoksiddismutazy v okislitel'nyh processah kletki i metod opredeleniya ee v biologicheskih materialah [The role of superoxide dismutase in the oxidative processes of the cell and the method of its determination in biological materials]. Laboratornoe Delo, 11, 678–680.

Christaki, E., Florou-Paneri, P., & Bonos, E. (2011). Microalgae: A novel ingredient in nutrition. International Journal of Food Sciences and Nutrition, 62(8), 794–799.

Costa, D. F. A., Quigley, S. P., Isherwood, P., McLennan, S. R., & Poppi, D. P. (2016). Supplementation of cattle fed tropical grasses with microalgae increases microbial protein production and average daily gain. Journal of Animal Science, 94(5), 2047–2058.

Del Campo, J. A., García-González, M., & Guerrero, M. G. (2007). Outdoor cultivation of microalgae for carotenoid production: Current state and perspectives. Applied Microbiology and Biotechnology, 74(6), 1163–1174.

Doucha, J., Lívanský, K., Kotrbáček, V., & Zachleder, V. (2009). Production of Chlorella biomass enriched by selenium and its use in animal nutrition: A review. Applied Microbiology and Biotechnology, 83(6), 1001–1008.

Flerova, E., & Bogdanova, A. (2014). The features of biochemical indices of strain Chlorella vulgaris IGF No. C-111, grown in closed system. The Journal of Microbiology, Biotechnology and Food Sciences, 3, 311.

Guccione, A., Biondi, N., Sampietro, G., Rodolfi, L., Bassi, N., & Tredici, M. R. (2014). Chlorella for protein and biofuels: From strain selection to outdoor cultivation in a Green Wall Panel photobioreactor. Biotechnology for Biofuels, 7(1), 84.

Hasegawa, T., Kimura, Y., Hiromatsu, K., Kobayashi, N., Yamada, A., Makino, M., Okuda, M., Sano, T., Nomoto, K., & Yoshikai, Y. (1997). Effect of hot water extract of Chlorella vulgaris on cytokine expression patterns in mice with murine acquired immunodeficiency syndrome after infection with Listeria monocytogenes. Immunopharmacology, 35(3), 273–282.

Huo, S., Wang, Z., Zhu, S., Zhou, W., Dong, R., & Yuan, Z. (2012). Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China. Bioresource Technology, 121, 76–82.

Karasuyama, H., Obata, K., Wada, T., Tsujimura, Y., & Mukai, K. (2011). Newly appreciated roles for basophils in allergy and protective immunity. Allergy, 66(9), 1133–1141.

Kim, Y. J., Jeong, S., Kwon, S., & Kim, M. K. (2009). Effect of Chlorella vulgaris intake on antioxidative capacity in rats oxidatively stressed with dietary cadmium. Food Science and Biotechnology, 18(5), 1055–1062.

Kondrahin, I. P. (2004). Metody veterinarnoj i klinicheskoj laboratornoj diagnostiki [Methods of veterinary and clinical laboratory diagnostics]. KolosS, Moscow (in Russian).

Korolyuk, M. A., Ivanova, L. K., Majorova, I. G., & Tokareva, V. A. (1988). Metod opredeleniya aktivnosti katalazy [Method for determination of catalase activity]. Klinicheskaya Laboratornaya Diagnostika, 4, 44–47.

Kotrbacek, V., Filka, J., Ingr, I., & Dvorak, J. (1995). The effect of application of the alga Chlorella vulgaris on the occurrence of the pork defects. Zivocisna Vyroba, 11(40), 519–522.

Krapivina, E. V., Ivanov, D. V., & Lifanova, J. V. (2011). Vlijanie raznyh doz probiotika “Tetralaktobakterin” na morfobiohimicheskie harakteristiki gomeostaza teljat [Influence of different doses of the probiotic "Tetralactobacterin" on the morphobiochemical characteristics of calves' homeostasis]. Vestnik Orlovskogo Gosudarstvennogo Agrarnogo Universiteta, 31(4), 41–44 (in Russian).

Krisanov, A. F., Gorbacheva, N. N., & Demin, V. V. (2014). Gematologicheskie pokazateli korov pri kruglogodovom odnotipnom kormlenii. [Hematological indices of cows with year-round feeding of the same type]. Vestnik Ul'janovskoj GSHA, 27, 107–109 (in Russian).

Lamminen, M., Halmemies-Beauchet-Filleau, A., Kokkonen, T., Simpura, I., Jaakkola, S., & Vanhatalo, A. (2017). Comparison of microalgae and rapeseed meal as supplementary protein in the grass silage based nutrition of dairy cows. Animal Feed Science and Technology, 234, 295–311.

Lee, S., Kang, H., Lee, H., Kang, M., & Park, Y. (2010). Six-week supplementation with Chlorella has favorable impact on antioxidant status in Korean male smokers. Nutrition, 26(2), 175–183.

Lee, Y. K. (2001). Microalgal mass culture systems and methods: Their limitation and potential. Journal of Applied Phycology, 13(4), 307–315.

Lum, K. K., Kim, J., & Lei, X. G. (2013). Dual potential of microalgae as a sustainable biofuel feedstock and animal feed. Journal of Animal Science and Biotechnology, 4(1), 53.

Makartsev, N. G. (2012). Kormlenie sel'skohozjajstvennyh zhivotnyh [Feeding of farm animals]. Noosfera, Kaluga (in Russian).

Nadarinskaya, M. A. (2004). Vlijanie razlichnyh urovnej selena na produktivnost' i gematologicheskie pokazateli korov s udoem 6–7 tys. kg za laktaciju [The effect of various levels of selenium on the productivity and haematological parameters of cows with milk yield of 6–7 thousand kg for lactation]. Zhivotnovodstvo, 8, 6–9 (in Russian).

Nikitin, V. N. (1949). Atlas kletok krovi sel'skohozjajstvennyh i laboratornyh zhivotnyh [Atlas of blood cells of agricultural and laboratory animals]. GISHL, Kharkov (in Russian).

Nisticò, G., Ciriolo, M. R., Fiskin, K., Iannone, M., de Martino, A., & Rotilio, G. (1992). NGF restores decrease in catalase activity and increases superoxide dismutase and glutathione peroxidase activity in the brain of aged rats. Free Radical Biology and Medicine, 12(3), 177–181.

Ogbonna, J. C., Masui, H., & Tanaka, H. (1997). Sequential heterotrophic/autotrophic cultivation – an efficient method of producing Chlorella biomass for health food and animal feed. Journal of Applied Phycology, 9(4), 359–366.

Oshurkova, Y. L., Fomina, L. L., Mekhanikova, M. V., & Soboleva, Y. N. (2015). Vlijanie kormovoj dobavki Chlorella na nekotorye pokazateli krovi teljat [Influence of fodder supplement of Chlorella on some parameters of calves' blood]. Molochnohozjajstvennyj Vestnik, 19, 47–52 (in Russian).

Panahi, Y., Darvishi, B., Jowzi, N., Beiraghdar, F., & Sahebkar, A. (2016). Chlorella vulgaris: A multifunctional dietary supplement with diverse medicinal properties. Current Pharmaceutical Design, 22(2), 164–173.

Panov, D. K., Patieva, A. M., & Koshchaev, A. G. (2016). Nekotorye biochipmicheskie pokazateli krovi molodnyaka krupnogo rogatogo skota pri vypojke suspenzii mikrovodorosli [Some biochemical indicators of the blood of young cattle during the digestion of a suspension of microalgae]. International Research Journal, 17, e85 (in Russian).

Pulz, O., & Gross, W. (2004). Valuable products from biotechnology of microalgae. Applied Microbiology and Biotechnology, 65(6), 635–648.

Ramos, A. L., Torello, C. O., & Queiroz, M. L. (2010). Chlorella vulgaris modulates immunomyelopoietic activity and enhances the resistance of tumor-bearing mice. Nutrition and Cancer, 62(8), 1170–1180.

Shibata, S., Natori, Y., Nishihara, T., Tomisaka, K., Matsumoto, K., Sansawa, H., & Nguyen, V. C. (2003). Antioxidant and anti-cataract effects of Chlorella on rats with streptozotocin-induced diabetes. Journal of Nutritional Science and Vitaminology, 49(5), 334–339.

Shibata, S., Oda, K., Onodera-Masuoka, N., Matsubara, S., Kikuchi-Hayakawa, H., Ishikawa, F., Iwabuchi, A., & Sansawa, H. (2001). Hypocholesterolemic effect of indigestible fraction of Chlorella regularis in cholesterol-fed rats. Journal of Nutritional Science and Vitaminology, 47(6), 373–377.

Shmigel', V. V., Fljorova, E. A., Bogdanova, A. A., & Suhovskij, N. A. (2015). Ustanovka dlja vyrashhivanija chlorelly [Installation for cultivation of Chlorella], Patent RF, No 2562867 (in Russian).

Shmigel', V. V., Fljorova, E. A., Bogdanova, A. A., & Suhovskij, N. A. (2015). Sposob vyrashhivanija chlorelly [Way of cultivation of Chlorella]. Patent RF, No 2558300 (in Russian).

Spolaore, P., Joannis-Cassan, C., Duran, E., & Isambert, A. (2006). Commercial applications of microalgae. Journal of Bioscience and Bioengineering, 101(2), 87–96.

Tanaka, K., Konishi, F., Himeno, K., Taniguchi, K., & Nomoto, K. (1984). Augmentation of antitumor resistance by a strain of unicellular green algae, Chlorella vulgaris. Cancer Immunology Immunotherapy, 17(2), 90–94.

Tanaka, K., Tomita, Y., Tsuruta, M., Konishi, F., Okuda, M., Himeno, K., & Nomoto, K. (1990). Oral administration of Chlorella vulgaris augments concomitant antitumor immunity. Immunopharmacology and Immunotoxicology, 12(2), 277–291.

Vijayavel, K., Anbuselvam, C., & Balasubramanian, M. P. (2007). Antioxidant effect of the marine algae Chlorella vulgaris against naphthalene-induced oxidative stress in the albino rats. Molecular and Cellular Biochemistry, 303(1–2), 39–44.

Viktorov, P. I., & Menkin, V. K. (1991). Metodika i organizacija zootehnicheskih opytov [Methods and organization of zootechnical experiments]. Agropromizdat, Moscow (in Russian).

Yan, L., Lim, S. U., & Kim, I. H. (2012). Effect of fermented Chlorella supplementation on growth performance, nutrient digestibility, blood characteristics, fecal microbial and fecal noxious gas content in growing pigs. Asian-Australasian Journal of Animal Sciences, 25(12), 1742.

Zheng, L., Oh, S. T., Jeon, J. Y., Moon, B. H., Kwon, H. S., Lim, S. U., & Kang, C. W. (2012). The dietary effects of fermented Chlorella vulgaris (CBT^®) on production performance, liver lipids and intestinal microflora in laying hens. Asian-Australasian Journal of Animal Sciences, 25(2), 261–266.