The role of neuro-specific dihydropyrimidinase-related protein 2 (dpyl2) in spatial memory formation in teleosts
AbstractThis article presents the results of an experiment on the influence of serotonin-modulating anticonsolidation protein (SMAP) on the spatial memory formation of juvenile goldfish Carassius auratus (L.) in a maze with food reinforcement. Three experimental fish groups were formed: (1) intact animals, (2) experimental group (fish injected ICV with SMAP in 24 h before the beginning of training; 2 μl, 1.5 mg·ml–1), (3) active control group (fish injected ICV with inactivated SMAP). Goldfishes of the experimental group demonstrated the lowest capability for spatial recognition: the maximum level of performance of the task was on 4th day of the training – 38%, while the values of this index in fishes of the control and intact groups were 70% and 63% respectively. In general, throughout the period of the training the average value of task performance was 16% in the SMAP-injected fish (in the control and intact groups – 42% and 53%, respectively). By using Ds-Na-polyacrylamide gel electrophoresis SMAP compositeon has been revealed. It was found that it consists of 10–12 protein components, among which four proteins dominated. They were identified by mass spectrometry MALDI-TOF: spectrin, dihydropyrimidinase-related protein 2 (DPYL2), tubulin and actin. It has been suggested that the most likely candidate responsible for the negative effects of SMAP on fish memory formation is DPYL2. It was hypothesized that anticonsolidation effect of SMAP is caused by the effect of DPYL2 which blocks the growth of axons or its cytostatic activity which leads to disorders in formation of new neurons in the brain as a result of learning.
Bakhshalieva, R. R., Mekhtiev, A. A., & Kasimov, R. Y. (2010). Participation of serotonin-modulated anticonsolidation protein in mediation of action of adverse factors on lipid peroxidation level in tissues of sturgeon fry Acipenser güldenstädti persicus. Journal of Evolutionary Biochemistry and Physiology, 46(5), 442–446.
Broglio, C., Gómez, A., Durán, E., Ocaña, F. M., Jiménez-Moya, F., Rodríguez, F., & Salas, C. (2005). Hallmarks of a common forebrain vertebrate plan: Specialized pallial areas for spatial, temporal and emotional memory in actinopterygian fish. Brain Research Bulletin, 66(4–6), 277–281.
Cognato, G. P., Bortolotto, J. W., Blazina, A. R., Christoff, R. R., Lara, D. R., Vianna, M. R., & Bonan, C. D. (2012). Y-maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods. Neurobiology of Learning and Memory, 98(4), 321–328.
Gaikwad, S., Stewart, A., Hart, P., Wong, K., Piet, V., Cachat, J., & Kalueff, A. V. (2011). Acute stress disrupts performance of zebrafish in the cued and spatial memory tests: The utility of fish models to study stress-memory interplay. Behavioral Processes, 87(2), 224–230.
Garina, D. V., & Mekhtiev, А. А. (2014). Effect of serotonin-modulated anticon-solidation protein on formation of long-term memory in the learning model of active avoidance in the carp (Cyprinus carpio). Journal of Evolutionary Biochemistry and Physiology, 50(1), 49–56.
Gasanov, G. G., & Mekhtiev, A. A. (1991). Detection of serotonin-modulating protein fraction and study of its participation in organization of the passive avoiding behavior. Bulletin of Experimental Biology and Medicine, 112(7), 5–7.
Guseinov, S. B., & Mekhtiev, A. A. (2013). Studies of the role of serotonin-modulating anticonsolidation protein in memory formation in rats in a shuttle box. Neuroscience and Behavioral Physiology, 43(5), 551–556.
Mekhtiev, A. A., Gaisina, A. A., Palatnikov, G. M., & Kasimov, R. Y. (2006). Decrease in activity of the serotoninergic system during mutagenesis. Bulletin of Experimental Biology and Medicine, 142(6), 611–613.
Mekhtiev, A. A., Gaisina, A. A., Voronezhskaya, E. E., Khabarova, M. Y., Gudratov, N. O., & Huseynov, S. B. (2016). Uchastie serotonin-moduliruemogo anticonsolidatsionnogo belka v regulyatsii razvitiya embrionov bol’shogo prudovika (Lymnaea stagnalis) i sarcomy l’yuisa u myshej gibridnoji liniji (FL C57B2/6 X DBA) [Engagement of serotonin-modulating anticonsolidation protein in regulation of embryogenesis of Lymnaea stagnalis and Lewis sarcoma in hybrid mice (FL C57B2/6 X DBA)]. Rossijskij Fiziologicheskij Zhurnal imeni I. M. Sechenova, 102(4), 490–499 (in Russian).
Mekhtiev, A. A., Kozyrev, S. A., Nikitin, V. P., & Sherstnev, V. V. (2003). Izbiratel'noe vliianie antitel k belku SMP-69 na aktivnost' komandnykh nejronov oboronitel'nogo povedenija vinogradnykh ulitok [Selective effect of the antibody to protein SMP-69 on activity of the defence behavior command neurons in grape snail]. Rossijskij Fiziologicheskij Zhurnal imeni I. M. Sechenova, 89, 389–396 (in Russian).
Mekhtiev, A. A., Panahova, E. N., Rashidova, A. F., & Guseinov, S. B. (2015). Engagement of serotonin-modulating anticonsolidation protein in memory formation and suppression of drug addiction and epileptic seizures. In: Li, M. D. (Ed.). New developments in serotonin research. Nova Science Publishers, New York. pp. 123–143.
Movsum-Zadeh, S. K., Mekhtiev, A. A., Mekhtiev, K. S., Telford, W. G., Gaisina, А. А., & Zey’nalov, S. K. (2013). Detoxikatsionnye svojstva serotonin-moduliruemogo anticonsolidatsionnogo belka v otnoshenii toxinov khimicheskoj i bacterial’noj prirody [Detoxic properties of serotonin-modulating anticonsolidation protein to toxins of chemical and bacterial origin]. Izvestiya NAN Azerbajdzhana. Seriya Biologicheskie i Meditsinskie Nauki, 68(1), 24–29 (in Russian).
Portavella, M., Vargas, J. P., Torres, B., & Salas, C. (2002). The effects of telencephalic pallial lesions on spatial, temporal, and emotional learning in goldfish. Brain Research Bulletin, 57(3–4), 397–399.
Rodriguez, F., Durán, E., Gómez, A., Ocaña, F. M., Ávarez, E., Jiménez-Moya, F., Broglio, C., & Salas, C. (2005). Cognitive and emotional functions of the teleost fish cerebellum. Brain Research Bulletin, 66(4–6), 365–370.
Sherstnev, V. V., Gruden', M. A., Golubeva, O. N., Solov’eva, O. A., & Aleksandrov, Y. I. (2015). Long-lived newly formed neurons in the mature brain are involved in the support of learning and memory processes. Journal of Neurochemistry, 9(1), 13–19.
Sherstnev, V. V., Yurasov, V. V., Storozheva, Z. I., Gruden', M. A., & Proshin, A. T. (2010). Neurogenesis and apoptosis in the mature brain during formation and consolidation of long-term memory. Journal of Neurochemistry, 4(2), 109–115.
Zion, B., Karplus, I., Grinshpon, J., Rosenfeld, L., & Barki, A. (2011). Periodic reinforcement of acoustically conditioned behavior in St. Peter’s fish, Sarotherodon galilaeus, for ranching purposes. Aquaculture, 315(3–4), 394–399.
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